Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,232,002	C→T	R273W (CGG→TGG)	umuC →	translesion error‑prone DNA polymerase V subunit; DNA polymerase activity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,232,002	0	C	T	100.0%	224.9 / NA	57	R273W (CGG→TGG)	umuC	translesion error‑prone DNA polymerase V subunit; DNA polymerase activity
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/30);  total (27/30)

CGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGAACCTTATTACGGCAATAGCGCGTCGGTAAAACTGCTGACGCCCACTCAGGACAGCAGGGAT  >  NC_000913/1231859‑1232148                                                                                                                                                |                                                                                                                                                   cGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCTGggcggc                                                                                                                                             >  2:725530/1‑151 (MQ=255)   gTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCTGGGcggcgg                                                                                                                                           >  2:878110/1‑151 (MQ=255)    tGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCTGGGcggcgg                                                                                                                                           >  1:855568/1‑150 (MQ=255)          aaCTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCTGGGCGGCGGAAAAACt                                                                                                                                    <  1:504563/151‑1 (MQ=255)           aCTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCTGGGCGGCGGAAAAACtt                                                                                                                                   <  2:855501/151‑1 (MQ=255)               cgcgGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCTGGGCGGCGGAAAAACTTCGCa                                                                                                                               <  1:132277/151‑1 (MQ=255)               cgcgGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCTGGGCGGCGGAAAAACTTCGCa                                                                                                                               <  2:271988/151‑1 (MQ=255)                     gAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCTGGGCGGCGGAAAAACTTCGCAGCGAGc                                                                                                                         <  2:560116/151‑1 (MQ=255)                      aaCCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCTGGGCGGCGGAAAAACTTCGCAGCGAGc                                                                                                                         >  2:117797/1‑150 (MQ=255)                       aCCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCTGGGCGGCGGAAAAACTTCGCAGCGAGCAt                                                                                                                       <  2:657661/151‑1 (MQ=255)                        cccTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCTGGGCGGCGGAAAAACTTCGCAGCGAGCATc                                                                                                                      >  1:761857/1‑151 (MQ=255)                         ccTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCTGGGCGGCGGAAAAACTTCGCAGCGAGCATCa                                                                                                                     <  1:235760/151‑1 (MQ=255)                               tGCAACTGGAAGAGTTTGCACCGACGAAGCAGGCAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCTGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATAtt                                                                                                                >  2:818054/1‑150 (MQ=255)                                gCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCTGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTg                                                                                                               <  2:519922/150‑1 (MQ=255)                                      ggAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCTGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGttt                                                                                                        >  2:122897/1‑151 (MQ=255)                                       gAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCTGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGttt                                                                                                        >  2:747720/1‑150 (MQ=255)                                       gAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCTGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGttt                                                                                                        >  1:568776/1‑150 (MQ=255)                                       gAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCTGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTa                                                                                                       <  1:793423/151‑1 (MQ=255)                                        aaGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCTGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTAt                                                                                                      <  2:559204/151‑1 (MQ=255)                                            gTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCTGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTcc                                                                                                  >  1:439034/1‑151 (MQ=255)                                            gTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCTGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTcc                                                                                                  <  1:21532/151‑1 (MQ=255)                                                gCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCTGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGt                                                                                              >  1:668173/1‑151 (MQ=255)                                                 cACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCTGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGtt                                                                                             <  1:488185/151‑1 (MQ=255)                                                     gacgaAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCTGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTttatt                                                                                         <  2:520236/151‑1 (MQ=255)                                                       cgaAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCTGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTttatta                                                                                        >  1:84951/1‑150 (MQ=255)                                                         aaGCAGTAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCTGTGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGGCGGTTTATCTCCGCGTTTATTgaga                                                                                     <  2:221181/151‑1 (MQ=255)                                                               gAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCTGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCAc                                                                               <  2:518878/151‑1 (MQ=255)                                                                aaaTTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCTGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCAcc                                                                              >  2:582357/1‑151 (MQ=255)                                                                   ttATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCTGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCAtt                                                                           >  1:468578/1‑151 (MQ=255)                                                                   ttATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCTGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCAtt                                                                           >  2:144929/1‑151 (MQ=255)                                                                    tATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCTGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCAttt                                                                          >  2:153304/1‑151 (MQ=255)                                                                    tATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCTGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCAtt                                                                           <  1:22992/150‑1 (MQ=255)                                                                     aTCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCTGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCAttt                                                                          <  1:46828/150‑1 (MQ=255)                                                                         gTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCTGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTg                                                                         <  2:702640/147‑1 (MQ=255)                                                                                  cGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCTGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGaa                                                              <  2:678253/149‑1 (MQ=255)                                                                                  cGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCTGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGAAcc                                                            >  1:14416/1‑151 (MQ=255)                                                                                       ggTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCTGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGAACCttatt                                                       <  1:423890/151‑1 (MQ=255)                                                                                         tGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCTGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGAACCTTATTAc                                                     >  1:582511/1‑151 (MQ=255)                                                                                             cGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCTGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGAACCTTATTACGGc                                                  >  2:896957/1‑150 (MQ=255)                                                                                               cATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCTGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGAACCTTATTACGGCAAt                                               >  2:29357/1‑151 (MQ=255)                                                                                                       aTTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCTGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGAACCTTATTACGGCAATAGCGCGTc                                       <  1:772426/151‑1 (MQ=255)                                                                                                         tATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCTGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGAACCTTATTACGGCAATAGCGCGTCgg                                     >  1:569036/1‑151 (MQ=255)                                                                                                          aTCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCTGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGAACCTTATTACGGCAATAGCGCGTCGGt                                    <  2:468531/151‑1 (MQ=255)                                                                                                              gTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCTGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGAACCTTATTACGGCAATAGCGCGTCGGTaaaa                                <  2:413507/151‑1 (MQ=255)                                                                                                                cGATGCGGCAGGCCATTTGTAGTTACGCTGCCTGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGAACCTTATTACGGCAATAGCGCGTCGGTAAAAct                              <  1:317800/151‑1 (MQ=255)                                                                                                                 gATGCGGCAGGCCATTTGTAGTTACGCTGCCTGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGAACCTTATTACGGCAATAGCGCGTCGGTAAAAct                              >  1:354113/1‑150 (MQ=255)                                                                                                                  aTGCGGCAGGCCATTTGTAGTTACGCTGCCTGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGAACCTTATTACGGCAATAGCGCGTCGGTAAAActgc                            >  2:771576/1‑151 (MQ=255)                                                                                                                    gCGGCAGGCCATTTGTAGTTACGCTGCCTGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGAACCTTATTACGGCAATAGCGCGTCGGTAAAActgct                           <  1:529257/150‑1 (MQ=255)                                                                                                                            ccATTTGTAGTTACGCTGCCTGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTc                                                                                                             >  2:459840/1‑60 (MQ=255)                                                                                                                            ccATTTGTAGTTACGCTGCCTGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTc                                                                                                             <  1:459887/60‑1 (MQ=255)                                                                                                                            ccATTTGTAGTTACGCTGCCTGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGAACCTTATTACGGCAATAGCGCGTCGGTAAAACTGCTGACGCCCAc                  >  1:173107/1‑151 (MQ=255)                                                                                                                              aTTTGTAGTTACGCTGCCTGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGAACCTTATTACGGCAATAGCGCGTCGGTAAAACTGCTGACGCCCACTc                >  1:434694/1‑151 (MQ=255)                                                                                                                                  gTAGTTACGCTGCCTGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGAACCTTATTACGGCAATAGCGCGTCGGTAAAACTGCTGACGCCCACTCAGGa            <  2:419952/151‑1 (MQ=255)                                                                                                                                    aGTTACGCTGCCTGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGAACCTTATTACGGCAATAGCGCGTCGGTAAAACTGCTGACGCCCACTCAGGAca          <  1:192623/151‑1 (MQ=255)                                                                                                                                    aGTTACGCTGCCTGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGAACCTTATTACGGCAATAGCGCGTCGGTAAAACTGCTGACGCCCACTCAGGAc           >  1:21692/1‑150 (MQ=255)                                                                                                                                         cGCTGCCTGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGAACCTAATTACGGCAATAGCGCGTCTTTAAAACTGCTGACGCCCACTCAGGCCAGCAgg     <  2:332103/151‑1 (MQ=255)                                                                                                                                             gCCTGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGAACCTTATTACGGCAATAGCGCGTCGGTAAAACTGCTGACGCCCACTCAGGACAGCAGGGat  <  2:173083/150‑1 (MQ=255)                                                                                                                                                |                                                                                                                                                   CGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGAACCTTATTACGGCAATAGCGCGTCGGTAAAACTGCTGACGCCCACTCAGGACAGCAGGGAT  >  NC_000913/1231859‑1232148

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑