Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,260,849	G→A	intergenic (‑46/+79)	dauA ← / ← prs	C4‑dicarboxylic acid transporter/phosphoribosylpyrophosphate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,260,849	0	G	A	98.6%	226.1 / NA	69	intergenic (‑46/+79)	dauA/prs	C4‑dicarboxylic acid transporter/phosphoribosylpyrophosphate synthase
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (31/37);  total (31/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATCAGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCAT  >  NC_000913/1260703‑1260993                                                                                                                                                   |                                                                                                                                                 aTCAGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAAGTGa                                                                                                                                              <  2:906761/151‑1 (MQ=255) aTCAGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAAGTGa                                                                                                                                              <  2:300233/151‑1 (MQ=255)   cAGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAAGTGaa                                                                                                                                             <  2:95314/150‑1 (MQ=255)   cAGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAAGTGa                                                                                                                                              >  2:280002/1‑149 (MQ=255)      gTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAAGTGAagg                                                                                                                                           >  2:68415/1‑149 (MQ=255)        cACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAAGTGAaggagg                                                                                                                                        <  2:593810/150‑1 (MQ=255)         aCGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAAGTGAAGGAGGCa                                                                                                                                      <  1:843968/151‑1 (MQ=255)               aaaCCGTGCGGCAGTATATTTTTCTTTCCAGCAAGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAAGTGAAGGAGGCATAGGTc                                                                                                                                <  2:112596/151‑1 (MQ=255)                 aCCGTGCGGCAGTATATTTTTCTTTCCAGCAAGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAAGTGAAGGAGGCATAGGTCAt                                                                                                                              >  2:865471/1‑151 (MQ=255)                  ccGTGCGGCAGTATATTTTTCTTTCCAGCAAGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAAGTGAAGGAGGCATAGGTCATa                                                                                                                             <  1:732462/151‑1 (MQ=255)                   cGTGCGGCAGTATATTTTTCTTTCCAGCAAGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAAGTGAAGGAGGCATAGGTCATAc                                                                                                                            >  1:741549/1‑151 (MQ=255)                    gTGCGGCAGTATATTTTTCTTTCCAGCAAGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAAGTGAAGGAGGCATAGGTCATACa                                                                                                                           <  1:840428/151‑1 (MQ=255)                      gCGGCAGTATATTTTTCTTTCCAGCAAGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAAGTGAAGGAGGCATAGGTCATACaa                                                                                                                          <  1:274032/150‑1 (MQ=255)                        ggCAGTATATTTTTCTTTCCAGCAAGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAAGTGAAGGAGGCATAGGTCATACAAATg                                                                                                                       >  2:601594/1‑151 (MQ=255)                          cAGTATATTTTTCTTTCCAGCAAGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAAGTGAAGGAGGCATAGGTCATACAAATGGa                                                                                                                     <  1:554721/151‑1 (MQ=255)                           aGTATATTTTTCTTTCCAGCAAGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAAGTGAAGGAGGCATAGGTCATACAAATGGat                                                                                                                    >  1:65295/1‑151 (MQ=255)                              atatTTTTCTTTCCAGCAAGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAAGTGAAGGAGGCATAGGTCATACAAATGGatat                                                                                                                  <  1:799163/150‑1 (MQ=255)                              atatTTTTCTTTCCAGCAAGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAAGTGAAGGAGGCATAGGTCATACAAATGGATAtt                                                                                                                 >  1:13516/1‑151 (MQ=255)                                atTTTTCTTTCCAGCAAGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAAGTGAAGGAGGCATAGGTCa                                                                                                                               >  2:1478/1‑135 (MQ=255)                                atTTTTCTTTCCAGCAAGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAAGTGAAGGAGGCATAGGTCa                                                                                                                               <  1:1478/135‑1 (MQ=255)                                atTTTTCTTTCCAGCAAGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAAGTGAAGGAGGCATAGGTCATACAAATGGATATTAc                                                                                                               <  2:744374/151‑1 (MQ=255)                                  ttttCTTTCCAGCAAGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAAGTGAAGGAGGCATAGGTCATACAAATGGATATTACa                                                                                                              <  1:770730/150‑1 (MQ=255)                                        ttcCAGCAAGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAAGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACaaa                                                                                                        >  1:37389/1‑150 (MQ=255)                                           cAGCAAGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAAGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACaaaaaa                                                                                                     >  2:551016/1‑150 (MQ=255)                                            aGCAAGCGTCGATCACAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAAGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAAc                                                                                                   >  1:234118/1‑151 (MQ=255)                                             gCAAGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAAGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAAcc                                                                                                  <  1:687385/151‑1 (MQ=255)                                             gCAAGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAAGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAAcc                                                                                                  <  2:794995/151‑1 (MQ=255)                                              cAAGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAAGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAAccc                                                                                                 <  2:294423/151‑1 (MQ=255)                                                    tCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAAGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCcgccgc                                                                                            >  2:626639/1‑150 (MQ=255)                                                     cGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAAGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCcgccgc                                                                                            >  2:465496/1‑149 (MQ=255)                                                          agagCGCGAAAAGGCATCACATGGGAGAAAAATATTTTGTTCAAAAAAATGTTACATCCGTGAGCGAATCATCTGTCAACAAAAGGGCAAGTGAAGGGGGCATAGGTCATACAAATGGATATTACAGAAAAAAAAACCCGCCGCAGCggg                                                                                      <  2:61061/150‑1 (MQ=255)                                                              cgcgGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAAGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCttt                                                                                 >  2:435447/1‑151 (MQ=255)                                                                    aaGCCATCACATTGGAGAAAAATATTTTGTTAACAATAATGTTTCACCCGTGAGCGAATCATCTGTCAACTAAATGGAAAGTGAGGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAACCCCGCCGCAGCGGGTCTTTGAGCCg                                                                           <  2:63749/151‑1 (MQ=255)                                                                         aTCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAAGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTc                                                                      >  2:582581/1‑151 (MQ=255)                                                                          tCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAAGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCg                                                                     >  1:735615/1‑151 (MQ=255)                                                                             caTGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAAGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGAtt                                                                  >  2:356584/1‑151 (MQ=255)                                                                              aTGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAAGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGAtt                                                                  >  1:833400/1‑150 (MQ=255)                                                                                  gAGGAAAATATTTTGTTCAAAAAAATGTTTCATCCGTGAGCGCATCATCTGTCAACAAAAGGGCAAGTGAAGGAGGCATGGGTCATACAAAGGGATATTAAAGAAAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTa                                                                 <  2:328188/147‑1 (MQ=255)                                                                                   agaaaaaTATTTTGTTCACAATAATTTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGACAAGAGAAGGGGGCATAGGTCATACAAAGGGATATTAAAGAAAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAgtgt                                                             <  2:167946/147‑1 (MQ=255)                                                                                      aaaaTATTTTGTTCAAAAAATTGTTTCATCCGTGGGCGGTGCATGTGCAAACAAAATGGCAAGGGAAGGGGGATTAGGCACGACAAGTGGATATTAAAGAAAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTc                                                           <  2:248013/149‑1 (MQ=255)                                                                                       aaaTATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAAGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGa                                                         >  1:221343/1‑150 (MQ=255)                                                                                         atatTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAAGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAAc                                                       >  2:6215/1‑150 (MQ=255)                                                                                            ttttttTCAAAAAAATGTTTCTTCCGTGGGCGGATCATCTGCAAACAAAATGGCAAGTGAAGGAGTCAAAGGTCTTAAAAATGTATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACAt                                                     <  2:908094/149‑1 (MQ=255)                                                                                                 ttCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAAGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCag                                                >  2:232797/1‑149 (MQ=255)                                                                                                 ttCAAAATAATTTTTCATCCGTGAGCGCATCATCTGTAAAATAAAGGGCAAGTGAAGGAGGCATGGGTCATAAAAATGGATATTACAGAAAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCaga                                               <  2:735581/150‑1 (MQ=255)                                                                                                   cacaATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAAGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCag                                                >  2:430965/1‑147 (MQ=255)                                                                                                    acaATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAAGGGCAAGTGAAGGAGGCATAGGTCATAAAAAGGGATATTACAGAAAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATc                                           <  1:182817/151‑1 (MQ=255)                                                                                                      aataatGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAAGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGAC‑AAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGAt                                        >  1:724319/1‑151 (MQ=255)                                                                                                        taatGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAAGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGGCAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGAtt                                       >  2:543115/1‑151 (MQ=255)                                                                                                        taatGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAAGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGAtt                                       >  1:438955/1‑151 (MQ=255)                                                                                                             tttCATCCGTGGGCGCATCATCTGTCAAATAAATGGCAAGTGAAGGAGGCATAGGTCATACAAATGGATATTACAAAAAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGAttcttc                                   <  1:468308/150‑1 (MQ=255)                                                                                                             tttCATCCGTGAGCGCATCATCTGTAAACTAAATGGCAAGTGAAGGAGGCATAGCTCATAAAAATGGATATTACAGACAAAAAACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGAttctt                                    <  1:520491/149‑1 (MQ=255)                                                                                                                  cccGTGAGCGCTTCATCTGTCAACTAAAGGGAAAGTGAAGGAGGCATAGGTCAAACAAATGGATATTCCAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCt                             <  2:104313/150‑1 (MQ=255)                                                                                                                   ccGTGAGCGAATCATCTGTCAACAAAATGGCAAGGGAAGGAGGCATAGGTCATAAAAATGGATATTACAAACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCt                             <  2:497160/150‑1 (MQ=255)                                                                                                                     gTGAGCGCATCATCTGTCAACAAAATGGCAAGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGa                           <  2:41829/150‑1 (MQ=255)                                                                                                                     gTGAGCGCATCATCTGTAAACTAAATGGCAAGGGAAGGAGGAATAGGTCATACAAATGGATATTACAGACAAAAAACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGAt                          <  1:651968/151‑1 (MQ=255)                                                                                                                      tGAGCGCATCATCTGTCAACTAAATGGCAAGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGAt                          >  1:109767/1‑150 (MQ=255)                                                                                                                        aGCGCATCATCTGTCAACTAAATGGCAAGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATacg                       >  1:669837/1‑151 (MQ=255)                                                                                                                                atcTGTCAACAAAATGGCAAGTGAAGGAGGCATAGGTCATACAAAGGAATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGaa                  <  1:68437/148‑1 (MQ=255)                                                                                                                                 tcTGTCAACAAAAGGGAAAGTGAAGGGGGAATAGGTCATAAAAAGGGATATTACAGAAAAAAAACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATc                <  2:169164/149‑1 (MQ=255)                                                                                                                                   tGTCAACTAAATGGCAAGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCtt            >  2:559746/1‑151 (MQ=255)                                                                                                                                    gTCAACTAAATGGCAAGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGAAAAAAAACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCg               <  1:372000/147‑1 (MQ=255)                                                                                                                                    gTCAACAAAATGGCAAGTGAAGGAGGAATAGGTAATAAAAATGGATATTACAGAAAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCt             <  1:490537/149‑1 (MQ=255)                                                                                                                                     tCAACTAAATGGCAAGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCg          >  2:541719/1‑151 (MQ=255)                                                                                                                                       aaCTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGAATATTCCAAAAAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCg               <  1:180484/144‑1 (MQ=255)                                                                                                                                       aaCTAAATGGAAAGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCg          <  1:6226/149‑1 (MQ=255)                                                                                                                                        aCTAAATGGAAAGTGAAGGAGGCATAGGTCATACAAATGGATATTACAAAAAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGcc       <  1:356610/151‑1 (MQ=255)                                                                                                                                         cTAAATGGCAAGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGcc       >  1:823858/1‑150 (MQ=255)                                                                                                                                              tGGCAAGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCAt  >  2:737809/1‑150 (MQ=255)                                                                                                                                                   |                                                                                                                                                 ATCAGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCAT  >  NC_000913/1260703‑1260993

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑