Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	233,559	T→C	T133A (ACG→GCG)	mltD ←	putative membrane‑bound lytic murein transglycosylase D

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	233,559	0	T	C	100.0%	218.9 / NA	64	T133A (ACG→GCG)	mltD	putative membrane‑bound lytic murein transglycosylase D
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (36/28);  total (36/28)

TTGTTCAGACGCTGCATCATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGTTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGTTACGTTTTTTAACTTGCCCTGCTATCCAGTACATATACGGCTCTGCCCGTAAAGTTACATCGTGGAGATAGCTCTTATTGCGTAA  >  NC_000913/233411‑233703                                                                                                                                                     |                                                                                                                                                 ttGTTCAGACGCTGCATCATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTg                                                                                                                                                >  1:143291/1‑151 (MQ=255)  tGTTCAGACGCTGCATCATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGc                                                                                                                                               >  2:64741/1‑151 (MQ=255)    ttCAGACGCTGCATCATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCg                                                                                                                                              >  2:64299/1‑150 (MQ=255)     tCAGACGCTGCATCATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGt                                                                                                                                             <  1:719462/150‑1 (MQ=255)      cAGACGCTGCATCATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGa                                                                                                                                           <  1:463987/151‑1 (MQ=255)       aGACGCTGCATCATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTg                                                                                                                                            >  1:641361/1‑149 (MQ=255)        gACGCTGCATCATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAgg                                                                                                                                         <  1:534572/151‑1 (MQ=255)          cGCTGCATCATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGAt                                                                                                                                       >  2:386528/1‑151 (MQ=255)            cTGCATCATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATc                                                                                                                                      >  2:280722/1‑150 (MQ=255)             tGCATCATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCa                                                                                                                                     >  2:672650/1‑150 (MQ=255)              gCATCATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCaa                                                                                                                                    <  1:370059/150‑1 (MQ=255)                atcatATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAg                                                                                                                                 <  1:750689/151‑1 (MQ=255)                  catATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAgcg                                                                                                                               >  1:55753/1‑151 (MQ=255)                   atatTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAgcgc                                                                                                                              >  1:41294/1‑151 (MQ=255)                     atTCAGCGCGGCAGTTATTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATCGGCGCCAGACGCTGCGTGAGGATCAAAAGCGgtc                                                                                                                            >  2:635181/1‑151 (MQ=255)                           cgcgGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACt                                                                                                                      >  2:655090/1‑151 (MQ=255)                              ggCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATg                                                                                                                   >  1:497358/1‑151 (MQ=255)                               gCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATgg                                                                                                                  >  1:656351/1‑151 (MQ=255)                                cAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATACTTGCGGGTCTGTTTCACACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATggg                                                                                                                 >  2:143827/1‑151 (MQ=255)                                    tgttgAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGgtagt                                                                                                             >  1:535989/1‑151 (MQ=255)                                         aaGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTAc                                                                                                        >  1:837757/1‑151 (MQ=255)                                             aacaacATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGt                                                                                                    >  1:259930/1‑151 (MQ=255)                                              acaacaTCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGtt                                                                                                   >  1:729765/1‑151 (MQ=255)                                                   aTCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATa                                                                                              <  2:321479/151‑1 (MQ=255)                                                     cgcgACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAgg                                                                                            >  1:14399/1‑151 (MQ=255)                                                       cgACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGATGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGc                                                                                           >  1:278040/1‑150 (MQ=255)                                                           gcgcgTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGtt                                                                                      <  2:744989/151‑1 (MQ=255)                                                            cgcgTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGTTa                                                                                     <  2:346417/151‑1 (MQ=255)                                                             gcgTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGTTAc                                                                                    >  2:465315/1‑151 (MQ=255)                                                              cgTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGTTACg                                                                                   <  1:64321/151‑1 (MQ=255)                                                                 cATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGTTACGttt                                                                                >  2:674897/1‑151 (MQ=255)                                                                  aTAACTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGTTACGtttt                                                                               >  2:748435/1‑151 (MQ=255)                                                                   tAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGTTACGttttt                                                                              >  2:8834/1‑151 (MQ=255)                                                                    aaTTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGTGTGATGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGTTACGtttttt                                                                             <  2:599256/151‑1 (MQ=255)                                                                    aaTTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGTTACGttttt                                                                              <  1:228043/150‑1 (MQ=255)                                                                    aaTTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGTTACGttttt                                                                              <  1:168758/150‑1 (MQ=255)                                                                     aTTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGTTACGTTTTTTa                                                                            <  1:72280/151‑1 (MQ=255)                                                                         cGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGTTACGTTTTTTAACt                                                                         <  2:127496/150‑1 (MQ=255)                                                                          gggTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGTTACGTTTTTTAACTTg                                                                       <  2:699307/151‑1 (MQ=255)                                                                                gTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGTTACGTTTTTTAACTTGCCCt                                                                   <  1:852714/149‑1 (MQ=255)                                                                                 tttCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGTTACGTTTTTTAACTTGCCCTGCt                                                                >  2:375334/1‑151 (MQ=255)                                                                                    cAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGTTACGTTTTTTAACTTGCCCTGCTAt                                                              <  2:187907/150‑1 (MQ=255)                                                                                    cAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGTTACGTTTTTTAACTTGCCCTGCTATc                                                             >  1:452897/1‑151 (MQ=255)                                                                                     aaaCCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGTTACGTTTTTTAACTTGCCCTGCTATcc                                                            >  2:289737/1‑151 (MQ=255)                                                                                     aaaCCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGTTACGTTTTTTAACTTGCCCTGCTATcc                                                            >  2:806131/1‑151 (MQ=255)                                                                                     aaaCCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGTTACGTTTTTTAACTTGCCCTGCTATcc                                                            <  1:655139/151‑1 (MQ=255)                                                                                       aCCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGTTACGTTTTTTAACTTGCCCTGCTATcc                                                            >  1:811069/1‑149 (MQ=255)                                                                                          aTAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGTTACGTTTTTTAACTTGCCCTGCTATCCAGTAc                                                       <  1:556084/151‑1 (MQ=255)                                                                                            aaTTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGTTACGTTTTTTAACTTGCCCTGCTATCCAGTACa                                                      <  2:443817/150‑1 (MQ=255)                                                                                             aTTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGTTACGTTTTTTAACTTGCCCTGCTATCCAGTACat                                                     >  1:27155/1‑150 (MQ=255)                                                                                                 cgcCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGTTACGTTTTTTAACTTGCCCTGCTATCCAGTACATATACg                                                <  1:15903/151‑1 (MQ=255)                                                                                                    cccGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGTTACGTTTTTTAACTTGCCCTGCTATCCAGTACATATACGGct                                             <  2:47520/151‑1 (MQ=255)                                                                                                    cccGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGTTACGTTTTTTAACTTGCCCTGCTATCCAGTACATATACGGct                                             >  2:799173/1‑151 (MQ=255)                                                                                                            cGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGTTACGTTTTTTAACTTGCCCTGCTATCCAGTACATATACGGCTCTGCCCg                                      <  2:811004/150‑1 (MQ=255)                                                                                                              gAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGTTACGTTTTTTAACTTGCCCTGCTATCCAGTACATATACGGCTCTGCCCGTaa                                   <  2:385487/151‑1 (MQ=255)                                                                                                                    tCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGTTACGTTTTTTAACTTGCCCTGCTATCCAGTACATATACGGCTCTGCCCGTAAAGTTAc                             >  2:308507/1‑151 (MQ=255)                                                                                                                       gCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGTTACGTTTTTTAACTTGCCCTGCTATCCAGTACATATACGGCTCTGCCCGTAAAGTTACa                            <  1:243416/149‑1 (MQ=255)                                                                                                                       gCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGTTACGTTTTTTAACTTGCCCTGCTATCCAGTACATATACGGCTCTGCCCGTAAAGTTACa                            <  2:314618/149‑1 (MQ=255)                                                                                                                            aTGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGTTACGTTTTTTAACTTGCCCTGCTATCCAGTACATATACGGCTCTGCCCGTAAAGTTACATCGTgg                      >  1:144291/1‑150 (MQ=255)                                                                                                                                cTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGTTACGTTTTTTAACTTGCCCTGCTATCCAGTACATATACGGCTCTGCCCGTAAAGTTACATCGTGGAGAt                  >  1:554253/1‑150 (MQ=255)                                                                                                                                          ggCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGTTACGTTTTTTAACTTGCCCTGCTATCCAGTACATATACGGCTCTGCCCGTAAAGTTACATCGTGGAGATAGCTCTTATTg       >  1:610754/1‑151 (MQ=255)                                                                                                                                           gcgcCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGTTACGTTTTTTAACTTGCCCTGCTATCCAGTACATATACGGCTCTGCCCGTAAAGTTACATCGTGGAGATAGCTCTTATTGc      >  1:145036/1‑151 (MQ=255)                                                                                                                                              ccAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGTTACGTTTTTTAACTTGCCCTGCTATCCAGTTCATATACGGCTCTGCCCGTAAAGTTACATCGTGGAGATAGCTCTTAtt        <  2:904010/146‑1 (MQ=255)                                                                                                                                               cAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGTTACGTTTTTTAACTTGCCCTGCTATCCAGTACATATACGGCTCTGCCCGTAAAGTTACATCGTGGAGATAGCTCTTATTGCGTaa  <  2:5209/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                 TTGTTCAGACGCTGCATCATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGTTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGTTACGTTTTTTAACTTGCCCTGCTATCCAGTACATATACGGCTCTGCCCGTAAAGTTACATCGTGGAGATAGCTCTTATTGCGTAA  >  NC_000913/233411‑233703

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑