Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,266,451	A→G	T120A (ACC→GCC)	prmC →	RF‑1 and RF‑2 N5‑glutamine methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,266,451	0	A	G	100.0%	151.0 / NA	44	T120A (ACC→GCC)	prmC	RF‑1 and RF‑2 N5‑glutamine methyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/26);  total (18/26)

TAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCGACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGTCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAGCGACTG  >  NC_000913/1266305‑1266596                                                                                                                                                   |                                                                                                                                                  tAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCGACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTGCCg                                                                                                                                                <  2:121494/150‑1 (MQ=255)      ggggTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCGACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCATATTCTCGATCTCGGGACGGGTGCCGGGGCg                                                                                                                                           >  1:575413/1‑150 (MQ=255)       gggTGCGAGAATTCTTGGCGTTGCCGTTATTTGTTTCGCCAGCGGCCTTAATTCCGGGCCCGGATACGGGGTTTCTGGTGGAGGAGGCACTGGCGGGGTTGGCTGAACAACCTTGCCCTTTTCCCGATCCCGGGACGGGTGCCGGGGCGAt                                                                                                                                         <  2:628119/151‑1 (MQ=255)              agaATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCGACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTGCCGGGGCGAttgcgctt                                                                                                                                  <  2:900642/151‑1 (MQ=255)              agaATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCGACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTGCCGGGGCGAttgcgctt                                                                                                                                  >  2:511438/1‑151 (MQ=255)              agaATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCGACCTTAACTCCGCGCCCGCATGCGGAGAGTGTGGAGGAGCAGGCACTGGCGCGCGTGCCGGAACAACCGTGCCGTCTTCTCGCTCTCGGGGTGGGGGCCGGGGGGAttgcgctt                                                                                                                                  >  2:248214/1‑151 (MQ=255)                      ggTCGTTGCCGTTATTTGTTTCGCCAGCGACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGc                                                                                                                          <  1:43004/151‑1 (MQ=255)                         cGTTGCCGTTATTTGTTTCGCCAGCGACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTag                                                                                                                       <  2:846307/151‑1 (MQ=255)                                     ttGTTTCGCCAGCGACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCgg                                                                                                            >  2:453431/1‑150 (MQ=255)                                         ttCGCCAGCGACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTg                                                                                                        <  2:438700/150‑1 (MQ=255)                                         ttCGCCAGCGACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTg                                                                                                        <  2:909890/150‑1 (MQ=255)                                               aGCGACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAAtt                                                                                                 <  2:579419/151‑1 (MQ=255)                                                  gACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATc                                                                                              >  2:470200/1‑151 (MQ=255)                                                  gACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATc                                                                                              >  1:178680/1‑151 (MQ=255)                                                             cgcgCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGt                                                                                         >  2:53546/1‑145 (MQ=255)                                                             cgcgCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCg                                                                                   <  1:560800/151‑1 (MQ=255)                                                                    ggATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCt                                                                            >  1:129357/1‑151 (MQ=255)                                                                     gATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTg                                                                           >  1:369250/1‑151 (MQ=255)                                                                       tACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGcc                                                                             >  2:221445/1‑147 (MQ=255)                                                                          ggAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCt                                                                      <  1:323004/151‑1 (MQ=255)                                                                               gtCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGtctc                                                                 <  1:768690/151‑1 (MQ=255)                                                                                   ggtggAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGTCTccc                                                               <  1:567042/149‑1 (MQ=255)                                                                                      ggAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGTCTCCCTGGc                                                           >  2:731476/1‑150 (MQ=255)                                                                                          cAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGTCTCCCTGGCACAAc                                                      >  2:564322/1‑151 (MQ=255)                                                                                                   gcgcGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGTCTCCCTGGCACAACGTAATGccc                                             <  2:172773/151‑1 (MQ=255)                                                                                                        gTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGTCTCCCTGGCACAACGTAATGCCCAGCAt                                        >  1:351625/1‑151 (MQ=255)                                                                                                             cTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGTCTCCCTGGCACAACGTAATGCCCAGCATCTgg                                    >  2:508256/1‑150 (MQ=255)                                                                                                              tGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGTCTCCCTGGCACAACGTAATGCCCAGCATCTGGCg                                  >  2:407118/1‑151 (MQ=255)                                                                                                               gAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGTCTCCCTGGCACAACGTAATGCCCAGCATCTGGCg                                  <  2:200459/150‑1 (MQ=255)                                                                                                               gAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGTCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGa                                 <  1:807864/151‑1 (MQ=255)                                                                                                                        tGCCGTATTCTCGATCTCGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGTCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCaaaaa                          <  2:532848/149‑1 (MQ=255)                                                                                                                        tGCCGTATTCTCGATCTCGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGTCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAat                         <  1:161391/150‑1 (MQ=255)                                                                                                                         gCCGTATTCTCGATCTCGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGTCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAatat                       <  2:526069/151‑1 (MQ=255)                                                                                                                              aTTCTCGATCTCGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGTCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCcac                   <  2:324886/150‑1 (MQ=255)                                                                                                                                 ctcGATCTCGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGTCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTc               >  2:333844/1‑151 (MQ=255)                                                                                                                                   cGATCTCGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGTCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACAtt                <  1:810773/148‑1 (MQ=255)                                                                                                                                   cGATCTCGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGTCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACAtt                <  1:453478/148‑1 (MQ=255)                                                                                                                                         cGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGTCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTg             <  2:563896/145‑1 (MQ=255)                                                                                                                                         cGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGTCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCaa          <  2:306230/148‑1 (MQ=255)                                                                                                                                         cGGGACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGTCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAg        <  2:515594/150‑1 (MQ=255)                                                                                                                                          gggACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGTCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTg             >  2:648217/1‑144 (MQ=255)                                                                                                                                          gggACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGTCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTg             <  1:648266/144‑1 (MQ=255)                                                                                                                                          gggACGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGTCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAGCg      >  1:455672/1‑151 (MQ=255)                                                                                                                                              cGGGTGCCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGTCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAGCGACTg  <  1:615631/151‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                  TAACCGGGGTGCGAGAATTCTGGTCGTTGCCGTTATTTGTTTCGCCAGCGACCTTAATTCCGCGCCCGGATACGGAGTGTCTGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGTCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAGCGACTG  >  NC_000913/1266305‑1266596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑