Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,302,933 C→T A12V (GCG→GTG)  oppB → oligopeptide ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,302,9330CT98.6% 255.0 / NA 69A12V (GCG→GTG) oppBoligopeptide ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (26/42);  total (27/42)
Fisher's exact test for biased strand distribution p-value = 3.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGATCATGAC  >  NC_000913/1302786‑1303080
                                                                                                                                                   |                                                                                                                                                   
ccGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGAt                                                                                                                                                  >  1:727260/1‑151 (MQ=255)
 cGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGAtt                                                                                                                                                 <  1:248727/151‑1 (MQ=255)
 cGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGAtt                                                                                                                                                 >  1:820229/1‑151 (MQ=255)
  ggAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTc                                                                                                                                                >  1:294874/1‑151 (MQ=255)
   gAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTcc                                                                                                                                               <  1:660487/151‑1 (MQ=255)
   gAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTcc                                                                                                                                               <  2:209366/151‑1 (MQ=255)
     atatGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCg                                                                                                                                              <  1:699612/150‑1 (MQ=255)
     atatGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGa                                                                                                                                             <  1:881539/151‑1 (MQ=255)
     atatGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGa                                                                                                                                             <  2:138785/151‑1 (MQ=255)
     atatGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGa                                                                                                                                             <  2:465222/151‑1 (MQ=255)
       atGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGAc                                                                                                                                            <  1:437262/150‑1 (MQ=255)
       atGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGAc                                                                                                                                            <  2:250392/150‑1 (MQ=255)
       atGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACg                                                                                                                                           <  1:766808/151‑1 (MQ=255)
       atGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACg                                                                                                                                           <  1:559757/151‑1 (MQ=255)
       atGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACg                                                                                                                                           <  2:266746/151‑1 (MQ=255)
           aCATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACGCtat                                                                                                                                       <  1:669356/151‑1 (MQ=255)
            cATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACGCtatt                                                                                                                                      <  2:379044/151‑1 (MQ=255)
            cATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACGCtatt                                                                                                                                      <  2:181316/151‑1 (MQ=255)
            cATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACGCtatt                                                                                                                                      >  2:524659/1‑151 (MQ=255)
            cATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACGCtatt                                                                                                                                      <  2:765122/151‑1 (MQ=255)
             aTTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACGCtattt                                                                                                                                     <  1:445123/151‑1 (MQ=255)
             aTTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACGCtattt                                                                                                                                     <  2:68053/151‑1 (MQ=255)
                      cACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACGCTATTTATTCttatt                                                                                                                            >  2:716656/1‑151 (MQ=255)
                       aCTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACGCTATTTATTCttatt                                                                                                                            >  2:136556/1‑150 (MQ=255)
                        cTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACGCTATTTATTCTTATTAc                                                                                                                          >  2:296391/1‑151 (MQ=255)
                           aTGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACGCTATTTATTCTTATTACTag                                                                                                                       <  2:798954/151‑2 (MQ=255)
                           aTGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACGCTATTTATTCTTATTACTAt                                                                                                                       >  2:295414/1‑151 (MQ=255)
                           aTGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACGCTATTTATTCTTATTACTAt                                                                                                                       >  2:307470/1‑151 (MQ=255)
                           aTGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACGCTATTTATTCTTATTACTAt                                                                                                                       <  1:118684/151‑1 (MQ=255)
                              gCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACGCTATTTATTCTTATTACTATTTc                                                                                                                    <  1:391126/151‑1 (MQ=255)
                               cAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACGCTATTTATTCTTATTACTATTTCg                                                                                                                   <  1:375673/151‑1 (MQ=255)
                                   aCGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCt                                                                                                               <  2:564878/151‑1 (MQ=255)
                                         ggCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTatg                                                                                                          >  2:252781/1‑150 (MQ=255)
                                          gCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTatgat                                                                                                        <  2:77431/151‑1 (MQ=255)
                                           cAGGAGTGTCCTGCGCCACGGTGGCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTATACGTCGATGTCTGGAAGTGATTCCGACGCTATTTAATCTTATTACTATTTCGTTCTTTatgatg                                                                                                       <  2:144606/151‑1 (MQ=255)
                                            aGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATgc                                                                                                      >  1:660307/1‑151 (MQ=255)
                                             ggAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATgcg                                                                                                     >  2:186315/1‑151 (MQ=255)
                                                  gtCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTcg                                                                                                <  1:103771/151‑1 (MQ=255)
                                                     cTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCgcgc                                                                                             >  2:289560/1‑151 (MQ=255)
                                                                 gtCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCtt                                                                                 >  2:908859/1‑151 (MQ=255)
                                                                     gATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCttt                                                                                <  2:758925/148‑1 (MQ=255)
                                                                        ttttATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCg                                                                           >  2:245375/1‑150 (MQ=255)
                                                                           tATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCg                                                                        <  2:822709/150‑1 (MQ=255)
                                                                                 aTTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTAc                                                                 >  1:817957/1‑151 (MQ=255)
                                                                                     cAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTa                                                             <  1:65044/151‑1 (MQ=255)
                                                                                        aaGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCg                                                          >  1:398362/1‑151 (MQ=255)
                                                                                         aGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGc                                                         >  2:478992/1‑151 (MQ=255)
                                                                                           gCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGcc                                                        >  2:206018/1‑150 (MQ=255)
                                                                                               aGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGa                                                     >  1:821757/1‑149 (MQ=255)
                                                                                                   cAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGAt                                               >  2:58797/1‑151 (MQ=255)
                                                                                                   cAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGAt                                               <  1:557975/151‑1 (MQ=255)
                                                                                                     gAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATgg                                             <  2:667155/151‑1 (MQ=255)
                                                                                                        ggTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCa                                          <  2:269584/151‑1 (MQ=255)
                                                                                                           aGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAat                                        <  2:379274/150‑1 (MQ=255)
                                                                                                           aGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAat                                        <  2:367077/150‑1 (MQ=255)
                                                                                                            gggCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAatat                                      >  1:527364/1‑151 (MQ=255)
                                                                                                            gggCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAatat                                      >  1:635729/1‑151 (MQ=255)
                                                                                                               cAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCg                                    >  1:480987/1‑150 (MQ=255)
                                                                                                                   gTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGc                                <  2:396665/150‑1 (MQ=255)
                                                                                                                    ttAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCg                               <  1:865693/150‑1 (MQ=255)
                                                                                                                          tttATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATatc                        >  1:517089/1‑151 (MQ=255)
                                                                                                                              ttCTACGTCGCTGTCTGGAAGTGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTa                                                                  >  1:321008/1‑105 (MQ=255)
                                                                                                                              ttCTACGTCGCTGTCTGGAAGTGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTa                                                                  <  2:320982/105‑1 (MQ=255)
                                                                                                                                   cGTCGCTGTCTGGAAGTGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATg               >  2:620386/1‑151 (MQ=255)
                                                                                                                                    gTCGCTGTCTGGAAGTGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGa              <  2:839427/151‑1 (MQ=255)
                                                                                                                                      cGCTGTCTGGAAGTGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGAt             <  1:312857/150‑1 (MQ=255)
                                                                                                                                          gTCTGGAAGTGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGAt        <  1:854643/151‑1 (MQ=255)
                                                                                                                                               gAAGTGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGATCATGa   <  1:808259/151‑1 (MQ=255)
                                                                                                                                                aaGTGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGATCATGac  <  2:480940/151‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGATCATGAC  >  NC_000913/1302786‑1303080

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: