Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,373,362 T→C N47N (AAT→AAC)  ycjO → putative sugar ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,373,3620TC100.0% 166.3 / NA 45N47N (AAT→AAC) ycjOputative sugar ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/16);  total (29/16)

AATCCGCAATGAACAGGCTTTTTTCAGGTCGTTCCGATATGCCCTTTGCGCTGCTGCTTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAATCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCTGGCATTCGCTGTGGATGACGGTCTGGTATACCGCGCTGGTGGTGGCGGGCAGCACCGTGCTCGGGCTGGCGGTGGCG  >  NC_000913/1373214‑1373506
                                                                                                                                                    |                                                                                                                                                
aaTCCGCAATGAACAGGCTTTTTTCAGGTCGTTCCGATATGCCCTTTGCGCTGCTGCTTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAAccc                                                                                                                                                >  2:324199/1‑151 (MQ=255)
aaTCCGCAATGAACAGGCTTTTTTCAGGTCGTTCCGATATGCCCTTTGCGCTGCTGCTTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAAccc                                                                                                                                                >  1:829098/1‑151 (MQ=255)
   ccGCAATGAACAGGCTTTTTTCAGGTCGTTCCGATATGCCCTTTGCGCTGCTGCTTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAACCCCa                                                                                                                                              >  2:128055/1‑150 (MQ=255)
      cAATGAACAGGCTTTTTTCAGGTCGTTCCGATATGCCCTTTGCGCTGCTGCTTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAACCCCAACa                                                                                                                                           <  2:88152/150‑1 (MQ=255)
         tGAACAGGCTTTTTTCAGGTCGTTCCGATATGCCCTTTGCGCTGCTGCTTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAACCCCAACATCg                                                                                                                                        >  1:393148/1‑150 (MQ=255)
           aaCAGGCTTTTTTCAGGTCGTTCCGATATGCCCTTTGCGCTGCTGCTTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAACCCCAACATCGAGt                                                                                                                                     >  1:648615/1‑151 (MQ=255)
               ggCTTTTTTCAGGTCGTTCCGATATGCCCTTTGCGCTGCTGCTTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAACCCCAACATCGAGTCAAc                                                                                                                                 >  1:271863/1‑151 (MQ=255)
               ggCTTTTTTCAGGTCGTTCCGATATGCCCTTTGCGCTGCTGCTTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAACCCCAACATCGAGTCAAc                                                                                                                                 <  1:72905/151‑1 (MQ=255)
                    ttttCAGGTCGTTCCGATATGCCCTTTGCGCTGCTGCTTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAACCCCAACATCGAGTCAACGTttg                                                                                                                            >  2:145071/1‑151 (MQ=255)
                           gTCGTTCCGATATGCCCTTTGCGCTGCTGCTTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAACCCCAACATCGAGTCAACGTTTGTTgggg                                                                                                                      >  2:37963/1‑150 (MQ=255)
                             cGTTCCGATATGCCCTTTGCGCTGCTGCTTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTg                                                                                                                    >  1:843253/1‑150 (MQ=255)
                             cGTTCCGATATGCCCTTTGCGCTGCTGCTTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTGa                                                                                                                   <  1:672938/151‑1 (MQ=255)
                              gTTCCGATATGCCCTTTGCGCTGCTGCTTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTg                                                                                                                    >  1:192808/1‑149 (MQ=255)
                                 ccGATATGCCCTTTGCGCTGCTGCTTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCaa                                                                                                               <  1:434015/151‑1 (MQ=255)
                                   gATATGCCCTTTGCGCTGCTGCTTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACt                                                                                                             >  2:402084/1‑151 (MQ=255)
                                         cccTTTGCGCTGCTGCTTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGc                                                                                                       <  1:765178/151‑1 (MQ=255)
                                         cccTTTGCGCTGCTGCTTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGGCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGc                                                                                                       <  2:306780/151‑1 (MQ=255)
                                          ccTTTGCGCTGCTGCTTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCg                                                                                                      >  2:279279/1‑151 (MQ=255)
                                             ttGCGCTGCTGCTTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTa                                                                                                    <  2:267757/150‑1 (MQ=255)
                                               gcgcTGCTGCTTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATcc                                                                                                 <  1:621635/151‑1 (MQ=255)
                                                cgcTGCTGCTTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCct                                                                                                <  2:192916/151‑1 (MQ=255)
                                                 gctgctgctTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCctc                                                                                               >  2:605299/1‑151 (MQ=255)
                                                   tgctgctTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCtctc                                                                                             >  1:485644/1‑151 (MQ=255)
                                                              gcgcCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCt                                                                                  >  2:7197/1‑151 (MQ=255)
                                                                   cAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCTgg                                                                             <  2:497584/151‑1 (MQ=255)
                                                                    aGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCTGGc                                                                            >  2:785376/1‑151 (MQ=255)
                                                                      cTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCTGGCAt                                                                          >  2:208245/1‑151 (MQ=255)
                                                                       ttattaCTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCTGGCAtt                                                                         >  2:601043/1‑151 (MQ=255)
                                                                            aCTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCTGGCATTCGCt                                                                     <  1:667373/150‑1 (MQ=255)
                                                                                  gggCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCTGGCATTCGCTGTGGa                                                                <  1:332594/149‑1 (MQ=255)
                                                                                         cTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCTGGCATTCGCTGTGGATGACGGTCt                                                       >  2:508014/1‑151 (MQ=255)
                                                                                           ggtggCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCTGGCATTCGCTGTGGATGACGGTc                                                        <  2:226804/148‑1 (MQ=255)
                                                                                              ggcgtggcCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCTGGCATTCGCTGTGGATGACGGTCTGGtat                                                  <  2:648136/151‑1 (MQ=255)
                                                                                                     ccGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCTGGCATTCGCTGTGGATGACGGTCTGGTATACCgcgc                                           >  1:399415/1‑151 (MQ=255)
                                                                                                             gtCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCTGGCATTCGCTGTGGATGACGGTCTGGTATACCGCGCtggtggtg                                   >  2:478724/1‑151 (MQ=255)
                                                                                                               cGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCTGGCATTCGCTGTGGATGACGGTCTGGTATACCGCGCTGGTGGTGGc                                 >  1:335257/1‑151 (MQ=255)
                                                                                                                gAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCTGGCATTCGCTGTGGATGACGGTCTGGTATACCGCGCTGGTGGTGGCg                                >  2:572278/1‑151 (MQ=255)
                                                                                                                 aaTATCGAAATCAGTTTTTTACGTCTGCCGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCTGGCATTCGCTGTGGATGACGGTCTGGTATACCGCGCTGGTGGTGGCgg                               >  1:2429/1‑151 (MQ=255)
                                                                                                                      cGAAATCAGTTTTTTACGTCTGCCGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCTGGCATTCGCTGTGGATGACGGTCTGGTATACCGCGCTGGTGGTGGCGGgc                             >  2:644188/1‑148 (MQ=255)
                                                                                                                       gAAATCAGTTTTTTACGTCTGCCGCTCAACCCCAACATCGAGTCAACGTTTGGTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCTGGCATTCGCTGTGGATGACGGTCTGGTATACCGCGCTGGTGGTGGCGGgcagc                          >  2:159798/1‑150 (MQ=255)
                                                                                                                         aaTCAGTTTTTTACGTCTGCCGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCTGGCATTCGCTGTGGATGACGGTCTGGTATACCGCGCTGGTGGTGGCGGGCAGCAcc                       >  2:192891/1‑151 (MQ=255)
                                                                                                                           tCAGTTTTTTACGTCTGCCGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCTGGCATTCGCTGTGGATGACGGTCTGGTATACCGCGCTGGTGGTGGCGGGCAGCACCg                      >  2:291593/1‑150 (MQ=255)
                                                                                                                                       gTCTGCCGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCTGGCATTCGCTGTGGATGACGGTCTGGTATACCGCGCTGGTGGTGGCGGGCAGCACCGTGCTCGGGCTGGc         <  2:61293/151‑1 (MQ=255)
                                                                                                                                         cTGCCGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCTGGCATTCGCTGTGGATGACGGTCTGGTATACCGCGCTGGTGGTGGCGGGCAGCACCGTGCTCGGGCTGGCgg       >  2:313163/1‑151 (MQ=255)
                                                                                                                                           gCCGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCTGGCATTCGCTGTGGATGACGGTCTGGTATACCGCGCTGGTGGTGGCGGGCAGCACCGTGCTCGGGCTGGCg        <  1:128077/148‑1 (MQ=255)
                                                                                                                                            ccGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCTGGCATTCGCTGTGGATGACGGTCTGGTATACCGCGCTGGTGGTGGCGGGCAGCACCGTGCTCGGGCTGGCGGTgg    >  1:557655/1‑151 (MQ=255)
                                                                                                                                              gCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCTGGCATTCGCTGTGGATGACGGTCTGGTATACCGCGCTGGTGGTGGCGGGCAGCACCGTGCTCGGGCTGGCGGTGGCg  >  2:128774/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
AATCCGCAATGAACAGGCTTTTTTCAGGTCGTTCCGATATGCCCTTTGCGCTGCTGCTTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAATCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCTGGCATTCGCTGTGGATGACGGTCTGGTATACCGCGCTGGTGGTGGCGGGCAGCACCGTGCTCGGGCTGGCGGTGGCG  >  NC_000913/1373214‑1373506

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑