Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	241,939	A→G	C455C (TGT→TGC)	fadE ←	acyl coenzyme A dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	241,939	0	A	G	100.0%	193.3 / NA	53	C455C (TGT→TGC)	fadE	acyl coenzyme A dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/31);  total (22/31)

GCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCGGTGCGCCCTGGTAAGCACGCGCCAGGAAGTTGCTTTGCCCGAGCATAATGCCTTTACCGCCGGTAATATCCATCGCATCAATAATCGACTGCTGCCCGCGGTGGGTACAGTGATACTTAACGATAGCCGACAGCACGGCAGGTTTTTCGCCGAGCATAATGCCGTAGGTAATCAGCGATGCCGCAGCATCCATCACGTAGGCATTACCGGCAATACGCGCCAGCGGCTCTTCAATCCCTTCCATCTTACCAATA  >  NC_000913/241792‑242086                                                                                                                                                    |                                                                                                                                                    gcGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCGGTGCGCCCTGTTAAGCACGCGCCAGGAAGTTGCTTTGCCCGAGCATAATGCCTTTACCGCCGGTAATATCCATCGCATCAATAATCGACTGCTGCCCGCGGTGGGTGCa                                                                                                                                                   <  1:832744/150‑1 (MQ=255) gcGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCGGTGCGCCCTGGTAAGCGCGCGCCAGGAAGTTGCTTTGCCCGAGCATAATGCCTTTACCGCCGGTAATATCCATCGCATCAATAATCGACTGCTGCCCGCGGTGGGTGCAg                                                                                                                                                  <  2:195912/151‑1 (MQ=255)  cGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCGGTGCGCCCTGGTAAGCACGCGCCAGGAAGTTGCTTTGCCCGAGCATAATGCCTTTACCGCCGGTAATATCCATCGCATCAATAATCGACTGCTGCCCGCGGTGGGTGCAGt                                                                                                                                                 >  1:227151/1‑151 (MQ=255)    ggTCAGAATGTTAGCCCCTTCAACGGGGAGGGCAATCGGTGCGCCCTGGTAAGCACGCGCCAGGAATTTGCTTTGCCCGAGCATAATGCCTTTACCGCCGGTAATATCCATCGCATCAATAATCGACTGCTGCCCGCGGTGGGTGCAGt                                                                                                                                                 <  2:441112/149‑1 (MQ=255)        aGAATGTTAGCCCCTTCAACGGTGATGGCAATCGGTGCGCCCTGGTAAGCACGCGCCAGGAAGTTGCTTTGCCCGAGCATAATGCCTTTACCGCCGGTAATATCCATCGCATCAATAATCGACTGCTGCCCGCGGTGGGTGCAGTGATACt                                                                                                                                           >  1:58227/1‑151 (MQ=255)               tAGCCCCTTCAACGGTGATGGCAATCGGTGCGCCCTGGTAAGCACGCGCCAGGAAGTTGCTTTGCCCGAGCATAATGCCTTTACCGCCGGTAATATCCATCGCATCAATAATCGACTGCTGCCCGCGGTGGGTGCAGTGATACTTAACGAt                                                                                                                                    >  1:775849/1‑151 (MQ=255)                aGCCCCTTCAACGGTGATGGCAATCGGTGCGCCCTGGTAAGCACGCGCCAGGAAGTTGCTTTGCCCGAGCATAATGCCTTTACCGCCGGTAATATCCATCGCATCAATAATCGACTGCTGCCCGCGGTGGGTGCAGTGATACTTAACGAt                                                                                                                                    >  1:907596/1‑150 (MQ=255)                  ccccTTCAACGGTGATGGCAATCGGTGCGCCCTGGTAAGCACGCGCCAGGAAGTTGCTTTGCCCGAGCATAATGCCTTTACCGCCGGTAATATCCATCGCATCAATAATCGACTGCTGCCCGCGGTGGGTGCAGTGATACTTAACGATAg                                                                                                                                  <  2:124139/150‑1 (MQ=255)                    ccTTCACCGGTGATGGCAATCGGTGCGCCCTGTTAGGCACGCGCCAGGAAGTGGCTTTGCCCGAGCATAATGCCTTTACCGCCGGTAATATCCATCGCATCAATAATCGACTGCTGCCCGCGGTGGGTGCAGTGATACTTAACGATAGcc                                                                                                                                <  2:700229/150‑1 (MQ=255)                       tCAACGGTGATGGCAATCGGTGCGCCCTGGTAAGCACGCGCCAGGAAGTTGCTTTGCCCGAGCATAATGCCTTTACCGCCGGTAATATCCATCGCATCAATAATCGACTGCTGCCCGCGGTGGGTGCAGTGATACTTAACGATAGCCGa                                                                                                                              <  2:28921/149‑1 (MQ=255)                           cGGTGATGGCAATCGGTGCGCCCTGGTAAGCACGCGCCAGGAAGTTGCTTTGCCCGAGCATAATGCCTTTACCGCCGGTAATATCCATCGCATCAATAATCGACTGCTGCCCGCGGTGGGTGCAGt                                                                                                                                                 >  2:810298/1‑126 (MQ=255)                           cGGTGATGGCAATCGGTGCCCCCTGGTAAGCACGCGCCAGGAAGTTGCTTTGCCCGAGCATAATGCCTTTACCGCCGGTAATATCCATCGCATCAATAATCGACTGCTGCCCGCGGTGGGTGCAGt                                                                                                                                                 <  1:810363/126‑1 (MQ=255)                                 tGGCAATCGGTGCGCCCTGGTAAGCACGCGCCAGGAAGTTGCTTTGCCCGAGCATAATGCCTTTACCGCCGGTAATATCCATCGCATCAATAATCGACTGCTGCCCGCGGTGGGTGCAGTGATACTTAACGATAGCCGACAGCACGGCAg                                                                                                                   <  2:511063/150‑1 (MQ=255)                                  ggCAATCGGTGCCCCCTGGTAAGCACGCGCCAGGAAGTTGTTTTGCCCGAGCATAATGCTTTTACCGCCGGTAATATCCATCGCATCAATAATCGACTGCTGCCCGCGGTGGGTGCAGTGATACTTAACGATAGCCGACAGCACGGCAGGt                                                                                                                 <  2:875786/151‑1 (MQ=255)                                   gCAATCGGTGCGCCCTGGTAAGCACGCGCCAGGAAGTTGCTTTGCCCGAGCATAATGCCTTTACCGCCGGTAATATCCATCGCATCAATAATCGACTGCTGCCCGCGGTGGGTGCAGTGATACTTAACGATAGCCGACAGCACGGCAg                                                                                                                   <  1:567469/148‑1 (MQ=255)                                       tCGGTGCGCCCTGGAAAGCACGCGCCAGAAAGTTGCTTTGCCCGAGCATAATGCCTTTACCGCCGGTAATATCCATCGCATCAATAATCGACTGCTGCCCGCGGTGGGTGCAGTGATACTTAACGATAGCCGACAGCACGGCAGGttttt                                                                                                             <  1:610467/150‑1 (MQ=255)                                             cgcCCTGGTAAGCCCGCGCCAGGAAGTTGCTTTGCCCGAGCATAAGGCCTTTACCGCCGGTAATATCCATCGCATCAATAATCGACTGCTGCCCGCGGTGGGTGCAGTGATACTTAACGATAGCCGACAGCACGGCAGGTTTTTCGCCGa                                                                                                       <  2:672682/150‑1 (MQ=255)                                                ccTGGTAAGCACGCGCCAGGAAGTTGCTTTGCCCGAGCATAATGCCTTTACCGCCGGTAATATCCATCGCATCAATAATCGACTGCTGCCCGCGGTGGGTGCAGTGATACTTAACGATAGCCGACAGCACGGCAGGTTTTTCGCCGAGCAt                                                                                                   >  2:489281/1‑151 (MQ=255)                                                  tGGAAAGCACGGGCCAGAAATTTCCTTTGCCCGAGCATAATGCCTTTACCGCCGGTAATATCCATCGCATCAATAATCGACTGCTGCCCGCGGTGGGTGCAGTGATACTTAACGATAGCCGACAGCACGGCAGGTTTTTCGCCGAGCa                                                                                                    <  2:760196/148‑1 (MQ=255)                                                     tAAGCACGCGCCAGGAAGTTGCTTTGCCCGAGCATAATGCCTTTACCGCCGGTAATATCCATCGCATCAATAATCGACTGCTGCCCGCGGTGGGTGCAGTGATACTTAACGATAGCCGACAGCACGGCAGGTTTTTCGCCGAGCATAATGc                                                                                              >  1:16730/1‑151 (MQ=255)                                                                cAGGAAGTTGCTTTGCCCGAGCATAATGCCTTTACCGCCGGTAATATCCATCGCATCAATAATCGACTGCTGCCCGCGGTGGGTGCAGTGATACTTAACGATAGCCGACAGCACGGCAGGTTTTTCGCCGAGCATAATGCCGTAGGTAATc                                                                                   <  2:230928/151‑1 (MQ=255)                                                                  ggAAGTTGTTTTGCCCGAGCATAATGCCTTTACCGCCGGTAATATCCATCGCATCAATAATCGACTGCTGCCCGCGGTGGGTGCAGTGATACTTAACGATAGCCGACAGCACGGCAGGTTTTTCGCCGAGCATAATGCCGTAGGTAATCAg                                                                                 <  2:548067/151‑1 (MQ=255)                                                                    aaGTTGCTTTGCCCGAGCATAATGCCTTTACCGCCGGTAATATCCATCGCATCAATAATCGACTGCTGCCCGCGGTGGGTGCAGTGATACTTAACGATAGCCGACAGCACGGCAGGTTTTTCGCCGAGCATAATGCCGTAGGTAATCAGc                                                                                <  1:220687/150‑1 (MQ=255)                                                                    aaGTTGCTTTGCCCGAGCATAATGCCTTTACCGCCGGTAATATCCATCGCATCAATAATCGACTGCTGCCCGCGGTGGGTGCAGTGATACTTAACGATAGCCGACAGCACGGCAGGTTTTTCGCCGAGCATAATGCCGTAGGTAATCAGCg                                                                               >  2:456543/1‑151 (MQ=255)                                                                         gCTTTGCCCAAACAAAATGCCTTTGCCGCCGGTAATATCCATCGCATCAATAATCGACTGCTGCCCGCGGTGGGTGCAGTGATACTTAACGATAGCCGACAGCACGGCAGGTTTTTCGCCGAGCATAATGCCGTAGGTAATCAGCGAt                                                                             <  2:463648/148‑1 (MQ=255)                                                                                 cGAGCATAATGCCTTTACCGCCGGTAATATCCATCGCATCAATAATCGACTGCTGCCCGCGGTGGGTGCAGTGATACTTAACGATAGCCGACAGCACGGCAGGTTTTTCGCCGAGCATAATGCCGTAGGTAATCAGCGATGCCGCAGcatc                                                                  >  2:575286/1‑151 (MQ=255)                                                                                     cATAATGCCTTTACCGCCGGTAATATCCATCGCATCAATAATCGACTGCTGCCCGCGGTGGGTGCAGTGATACTTAACGATAGCCGACAGCACGGCAGGTTTTTCGCCGAGCATAATGCCGTAGGTAATCAGCGATGCCGCAGcatccatc                                                              >  2:715317/1‑151 (MQ=255)                                                                                     cATAATGCCTTTACCGCCGGTAATATCCATCGCATCAATAATCGACTGCTGCCCGCGGTGGGTGCAGTGATACTTAACGATAGCCGACAGCACGGCAGGTTTTTCGCCGAGCATAATGCCGTAGGTAATCAGCGATGCCGCAGcatccatc                                                              >  1:800957/1‑151 (MQ=255)                                                                                        aagccttgtACCGCCGGTAATATCCATCGCATCAATAATCGACTGCTGCCCGCGGTGGGTGCAGTGATACTTAACGATAGCCGACAGCACGGCAGGTTTTTCGCCGAGCATAATGCCGTAGGTAATCAGCGATGCCGCAGCATCCATCa                                                             <  2:801945/141‑1 (MQ=255)                                                                                               ttACCGCCGGTAATATCCATCGCATCAATAATCGACTGCTGCCCGCGGTGGGTGCAGTGATACTTAACGATAGCCGACAGCACGGCAGGTTTTTCGCCGAGCATAATGCCGTAGGTAATCAGCGATGCCGCAGCATCCATCACGTAGGCAt                                                    >  1:400148/1‑151 (MQ=255)                                                                                                 aCCGCCGGTAATATCCATCGCATCAATAATCGACTGCTGCCCGCGGTGGGTGCAGTGATACTTAACGATAGCCGACAGCACGGCAGGTTTTTCGCCGAGCATAATGCCGTAGGTAATCAGCGATGCCGCAGCATCCATCACGTAGGCAtt                                                   <  2:243323/150‑1 (MQ=255)                                                                                                    gccgGTAATATCCATCGCATCAATAATCGACTGCTGCCCGCGGTGGGTGCAGTGATACTTAACGATAGCCGACAGCACGGCAGGTTTTTCGCCGAGCATAATGCCGTAGGTAATCAGCGATGCCGCAGCATCCATCACGTAGGCATTACCg                                               >  2:696112/1‑151 (MQ=255)                                                                                                     ccgGTAATATCCATCGCATCAATAATCGACTGCTGCCCGCGGTGGGTGCAGTGATACTTAACGATAGCCGACAGCACGGCAGGTTTTTCGCCGAGCATAATGCCGTAGGTAATCAGCGATGCCGCAGCATCCATCACGCAGGCATTACCgg                                              >  2:247093/1‑151 (MQ=255)                                                                                                         tAATATCCATCGCATCAATAATCGACTGCTGCCCGCGGTGGGTGCAGTGATACTTAACGATAGCCGACAGCACGGCAGGTTTTTCGCCGAGCATAATGCCGTAGGTAATCAGCGATGCCGCAGCATCCATCACGTAGGCATTACCGGCAAt                                          >  1:871686/1‑151 (MQ=255)                                                                                                           atatCCATCGCATCAATAATCGACTGCTGCCCGCGGTGGGTGCAGTGATACTTAACGATAGCCGACAGCACGGCAGGTTTTTCGCCGAGCATAATGCCGTAGGTAATCAGCGATGCCGCAGCATCCATCACGTAGGCATTACCGGCAATAc                                        >  2:902773/1‑151 (MQ=255)                                                                                                                cATCGCATCAATAATCGACTGCTGCCCGCGGTGGGTGCAGTGATACTTAACGATAGCCGACAGCACGGCAGGTTTTTCGCCGAGCATAATGCCGTAGGTAATCAGCGATGCCGCAGCATCCATCACGTAGGCATTACCGGCAATACGCGcc                                   >  2:28305/1‑151 (MQ=255)                                                                                                                cATCGCATCAATAATCGACTGCTGCCCGCGGTGGGTGCAGTGATACTTAACGATAGCCGACAGCACGGCAGGTTTTTCGCCGAGCATAATGCCGTAGGTAATCAGCGATGCCGCAGCATCCATCACGTAGGCATTACCGGCAATACGCGcc                                   >  2:396843/1‑151 (MQ=255)                                                                                                                   cGCATCAATAATCGACTGCTGCCCGCGGTGGGTGCAGTGATACTTAACGATAGCCGACAGCACGGCAGGTTTTTCGCCGAGCATAATGCCGTAGGTAATCAGCGATGCCGCAGCATCCATCACGTAGGCATTACCGGCAATACGCGCCAGc                                <  2:870708/151‑1 (MQ=255)                                                                                                                        cAATAATCGACTGCTGCCCGCGGTGGGTGCAGTGATACTTAACGATAGCCGACAGCACGGCAGGTTTTTCGCCGAGCATAATGCCGTAGGTAATCAGCGATGCCGCAGCATCCATCACGTAGGCATTACCGGCAATACGCGCCAGCGGctc                           >  2:629865/1‑151 (MQ=255)                                                                                                                          ataatCGACTGCTGCCCGCGGTGGGTGCAGTGATACTTAACGATAGCCGACAGCACGGCAGGTTTTTCGCCGAGCATAATGCCGTAGGTAATCAGCGATGCCGCAGCATCCATCACGTAGGCATTACCGGCAATACGCGCCAGCGGCTCtt                         <  1:907065/151‑1 (MQ=255)                                                                                                                               cGACTGCTGCCCGCGGTGGGTGCAGTGATACTTAACGATAGCCGACAGCACGGCAGGTTTTTCGCCGAGCATAATGCCGTAGGTAATCAGCGATGCCGCAGCATCCATCACGTAGGCATTACCGGCAATACGCGCCAGCGGCTCTTCAAt                     <  2:330006/150‑1 (MQ=255)                                                                                                                               cGACTGCTGCCCGCGGTGGGTGCAGTGATACTTAACGATAGCCGACAGCACGGCAGGTTTTTCGCCGAGCATAATGCCGTAGGTAATCAGCGATGCCGCAGCATCCATCACGTAGGCATTACCGGCAATACGCGCCAGCGGCTCTTCAATc                    >  1:111794/1‑151 (MQ=255)                                                                                                                                gACTGCTGCCCGCGGTGGGTGCAGTGATACTTAACGATAGCCGACAGCACGGCAGGTTTTTCGCCGAGCATAATGCCGTAGGTAATCAGCGATGCCGCAGCATCCATCACGTAGGCATTACCGGCAATACGCGCCAGCGGCTCTTCAATcc                   >  1:511932/1‑151 (MQ=255)                                                                                                                                    gctgcCCGCGGTGGGTGCAGTGATACTTAACGATAGCCGACAGCACGGCAGGTTTTTCGCCGAGCATAATGCCGTAGGTAATCAGCGATGCCGCAGCATCCATCACGTAGGCATTACCGGCAATACGCGCCAGCGGCTCTTCAATCCCTTc               <  1:839938/151‑1 (MQ=255)                                                                                                                                       gcCCGCGGTGGGTGCAGTGATACTTAACGATAGCCGACAGCACGGCAGGTTTTTCGCCGAGCATAATGCCGTAGGTAATCAGCGATGCCGCAGCATCCATCACGTAGGCATTACCGGCAATACGCGCCAGCGGCTCTTCAATCCCTTCCAt            <  2:800640/151‑1 (MQ=255)                                                                                                                                          cgcgGTGGGTGCAGTGATACTTAACGATAGCCGACAGCACGGCAGGTTTTTCGCCGAGCATAATGCCGTAGGTAATCAGCGATGCCGCAGCATCCATCACGTAGGCATTACCGGCAATACGCGCCAGCGGCTCTTCAATCCCTTCCATCtt         <  2:335268/151‑1 (MQ=255)                                                                                                                                          cgcgGTGGGTGCAGTGATACTTAACGATAGCCGACAGCACGGCAGGTTTTTCGCCGAGCATAATGCCGTAGGTAATCAGCGATGCCGCAGCATCCATCACGTAGGCATTACCGGCAATACGCGCCAGCGGCTCTTCAATCCCTTCCATCtt         <  1:413738/151‑1 (MQ=255)                                                                                                                                          cgcgGTGGGTGCAGTGATACTTAACGATAGCCGACAGCACGGCAGGTTTTTCGCCGAGCATAATGCCGTAGGTAATCAGCGATGCCGCAGCATCCATCACGTAGGCATTACCGGCAATACGCGCCAGCGGCTCTTCAATCCCTTCCATCtt         <  1:503093/151‑1 (MQ=255)                                                                                                                                              gTGGGTGCAGTGATACTTAACGATAGCCGACAGCACGGCAGGTTTTTCGCCGAGCATAATGCCGTAGGTAATCGGCGATGCCGCAGCATCCATCACGTAGGCATTACCGGCAATACGCGCCAGCGGCTCTTCAATCCCTTCCATCTTAcc      >  2:90348/1‑150 (MQ=255)                                                                                                                                              gTGGGTGCAGTGATACTTAACGATAGCCGACAGCACGGCAGGTTTTTCGCCGAGCATAATGCCGTAGGTAATCAGCGATGCCGCAGCATCCATCACGTAGGCATTACCGGCAATACGCGCCAGCGGCTCTTCAATCCCTTCCATCTTACCa     <  2:831523/151‑1 (MQ=255)                                                                                                                                                gggTGCAGTGATACTTAACGATAGCCGACAGCACGGCAGGTTTTTCGCCGAGCATAATGCCGTAGGTAATCAGCGATGCCGCAGCATCCATCACGTAGGCATTACCGGCAATACGCGCCAGCGGCTCTTCAATCCCTTCCATCTTACCaa    <  1:309287/150‑1 (MQ=255)                                                                                                                                                gggTGCAGTGATACTTAACGATAGCCGACAGCACGGCAGGTTTTTCGCCGAGCATAATGCCGTAGGTAATCAGCGATGCCGCAGCATCCATCACGTAGGCATTACCGGCAATACGCGCCAGCGGCTCTTCAATCCCTTCCATCTTACCaa    <  2:907523/150‑1 (MQ=255)                                                                                                                                                 ggTGCAGTGATACTTAACGATAGCCGACAGCACGGCAGGTTTTTCGCCGAGCATAATGCCGTAGGTAATCAGCGATGCCGCAGCATCCATCACGTAGGCATTACCGGCAATACGCGCCAGCGGCTCTTCAATCCCTTCCATCTTACCAATa  <  2:227125/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                    GCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCGGTGCGCCCTGGTAAGCACGCGCCAGGAAGTTGCTTTGCCCGAGCATAATGCCTTTACCGCCGGTAATATCCATCGCATCAATAATCGACTGCTGCCCGCGGTGGGTACAGTGATACTTAACGATAGCCGACAGCACGGCAGGTTTTTCGCCGAGCATAATGCCGTAGGTAATCAGCGATGCCGCAGCATCCATCACGTAGGCATTACCGGCAATACGCGCCAGCGGCTCTTCAATCCCTTCCATCTTACCAATA  >  NC_000913/241792‑242086

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑