| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,435,623 | A→G | intergenic (‑4/+137) | uspF ← / ← ompN | stress‑induced protein, ATP‑binding protein/outer membrane pore protein N, non‑specific |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,435,623 | 0 | A | G | 96.0% | 165.8 / NA | 50 | intergenic (‑4/+137) | uspF/ompN | stress‑induced protein, ATP‑binding protein/outer membrane pore protein N, non‑specific |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (18/30); minor base C (0/2); total (18/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.30e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
AGGCATAGTAGGGCAGTGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGA > NC_000913/1435475‑1435769 | atgCATAGTAGGGCAGTGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGcc < 2:509768/149‑1 (MQ=255) gtagGGCAGTGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGtt > 1:85572/1‑151 (MQ=255) agGGCAGTGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGtttt < 1:502855/151‑1 (MQ=255) cAGTGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGt > 2:859833/1‑150 (MQ=255) aGTGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTAt > 1:197095/1‑151 (MQ=255) gAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCg < 2:315861/151‑1 (MQ=255) ccGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAgtgt < 2:730734/151‑1 (MQ=255) aGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGc < 1:839861/151‑1 (MQ=255) ggAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGcc < 2:902598/151‑1 (MQ=255) aaaTGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGcc > 2:722622/1‑149 (MQ=255) aaCCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGac < 1:339261/151‑1 (MQ=255) aCCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGaca < 1:159866/151‑1 (MQ=255) ccTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGacac > 1:128184/1‑151 (MQ=255) gCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACttttt < 2:900323/151‑1 (MQ=255) gCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACtttt < 1:15663/150‑1 (MQ=255) gCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACtttt < 2:555968/150‑1 (MQ=255) gCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACtt < 1:98008/148‑1 (MQ=255) atcatcAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTG‑TTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTtgtg < 2:682504/151‑1 (MQ=255) ttGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACgg > 2:853108/1‑150 (MQ=255) ccTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGtt < 2:816830/151‑1 (MQ=255) ctgccTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATAGCGATAGGGACATGAAGCGTTCTGTTCATAAAGCCTGCTGTTTTATTGTCCGCATAAAGGGTAACGCCAGATGACGCTTTTTGTGTAATGACGGAGtta < 2:247674/146‑2 (MQ=255) ctTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTc < 2:706939/149‑1 (MQ=255) ttCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTcac > 1:204416/1‑150 (MQ=255) tCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTcaca < 2:31784/150‑1 (MQ=255) tCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTca < 2:909762/148‑1 (MQ=255) gCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAg < 1:640120/151‑1 (MQ=255) aaTCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGa < 2:902337/149‑1 (MQ=255) aaTCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATc < 1:263650/151‑1 (MQ=255) aTCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATc > 2:97427/1‑150 (MQ=255) tCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCa > 1:219529/1‑150 (MQ=255) cACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCaa < 1:92547/150‑1 (MQ=255) cgcgTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAagg > 2:632380/1‑151 (MQ=255) tAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAAt > 2:616838/1‑150 (MQ=255) aaTTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATaa < 1:811732/151‑1 (MQ=255) aTTCTGAATCTGGAATTTCGATAGTGACAAGAATCGTTCTGTTCATAAAGCCACCTGTTTTAGTATCCGTATAAAGTGTAACTCCAGATGACACTTTTTGTTTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGTAGGAAGAATaa < 2:746688/150‑1 (MQ=255) gAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGa < 1:655867/150‑1 (MQ=255) aaTCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGaaa < 1:632429/151‑1 (MQ=255) aaTCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGaaa < 1:180151/151‑1 (MQ=255) aTCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGaaaa < 1:701562/151‑1 (MQ=255) cGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATGAAGTGTAACGCCAGATGACACGTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGTGGAAGAGTAAGCACaaaaa < 2:492394/150‑1 (MQ=255) atCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGcc > 2:671012/1‑150 (MQ=255) atCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGcc > 2:523548/1‑150 (MQ=255) ggACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCg > 2:636204/1‑151 (MQ=255) aaGAATCTTTCTGTTCATAAACCCTCTTTTTTTAGTACCCGAAAAAAGTGAACCGCCAGATGACATTTTTTGTGTAATGACGGATTTCACTTTTTTAATTAAGAAAAAAGGAGAAAAAAAAAGAAAAAAAAGCCCGCCATAACAGCGGGCa < 2:802743/151‑1 (MQ=255) cGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCaggagg > 1:185827/1‑150 (MQ=255) ttCTGTTCATAAAGCCCCTTTTTTTAGTATCCTAATAAAGTGTACCGCAAGATGACATTTTTTGTGTAAGGCGGGAGTTCTCATTTTTAATTAAAACAAAAGGAGAAAGAATAAGAAAAAAAAGCCCGCCATAACAGCGGGCaggagg < 2:864787/148‑1 (MQ=255) gTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGAttt > 1:660488/1‑148 (MQ=255) gTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGAttt > 1:260425/1‑148 (MQ=255) ttCATAAACCCTCTTGTTTTAGTATCCGAATAAAGTGTAACGCAAGATGACATTTTTTGTGTAATGAGGGAGTTCACATTTTTAATTAAGAAAAAAGGAGGAAAAAAAAGCAAAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGa < 2:128162/150‑1 (MQ=255) tCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGaa > 2:401513/1‑150 (MQ=255) aaagCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGa > 1:694887/1‑150 (MQ=255) | AGGCATAGTAGGGCAGTGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGA > NC_000913/1435475‑1435769 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |