Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,474,495	A→G	D92G (GAC→GGC)	ydbC →	putative NAD(P)‑binding oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,474,495	0	A	G	100.0%	178.6 / NA	52	D92G (GAC→GGC)	ydbC	putative NAD(P)‑binding oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/23);  total (29/23)

AGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCACGTCACCAATCAGATTATCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGACGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACGTAATCTCGGGCTGGACGTGCTGGATGTGGTTAACCTGCGCGTTATGATGGGGGATGGTCATGGCCCAGCGGAAGGATCGATT  >  NC_000913/1474351‑1474640                                                                                                                                                 |                                                                                                                                                  aGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCACGTCACCAATCAGATTATCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATc                                                                                                                                             >  1:648601/1‑151 (MQ=255)    cgcTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCACGTCACCAATCAGATTATCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTg                                                                                                                                          >  2:85760/1‑151 (MQ=255)    cgcTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCACGTCACCAATCAGATTATCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTg                                                                                                                                          >  2:829659/1‑151 (MQ=255)    cgcTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCACGTCACCAATCAGATTATCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTg                                                                                                                                          <  2:747813/151‑1 (MQ=255)    cgcTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCACGTCACCAATCAGATTATCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTg                                                                                                                                          >  1:340947/1‑151 (MQ=255)             tGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCACGTCACCAATCAGATTATCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGc                                                                                                                                 >  2:654971/1‑151 (MQ=255)               ggCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCACGTCACCAATCAGATTATCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCAt                                                                                                                               >  1:44085/1‑151 (MQ=255)                    cAATCATATTGATACCAGCGACTTTTATGGTCCGCACGTCACCAATCAGATTATCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTtct                                                                                                                          >  1:472995/1‑151 (MQ=255)                     aaTCATATTGATACCAGCGACTTTTATGGTCCGCACGTCACCAATCAGATTATCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTtctc                                                                                                                         >  1:267299/1‑151 (MQ=255)                           atTGATACCAGCGACTTTTATGGTCCGCACGTCACCAATCAGATTATCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCa                                                                                                                    <  2:906863/150‑1 (MQ=255)                           atTGATACCAGCGACTTTTATGGTCCGCACGTCACCAATCAGATTATCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCa                                                                                                                    <  2:346265/150‑1 (MQ=255)                               aTACCAGCGACTTTTATGGTCCGCACGTCACCAATCAGATTATCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCt                                                                                                               >  1:111308/1‑151 (MQ=255)                                  ccAGCGACTTTTATGGTCCGCACGTCACCAATCAGATTATCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCa                                                                                                            <  1:301787/151‑1 (MQ=255)                                      cGACTTTTATGGTCCGCACGTCACCAATCAGATTATCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCaaaa                                                                                                         >  2:643512/1‑150 (MQ=255)                                       gACTTTTATGGTCCGCACGTCACCAATCAGATTATCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCaaaa                                                                                                         >  2:618667/1‑149 (MQ=255)                                       gACTTTTATGGTCCGCACGTCACCAATCAGATTATCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAg                                                                                                       <  1:342306/151‑1 (MQ=255)                                       gACTTTTATGGTCCGCACGTCACCAATCAGATTATCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAg                                                                                                       <  1:493037/151‑1 (MQ=255)                                          ttttCTGGTCCGCACGTCACCAATCAGATTATCCGCGAAGCTCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAACATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCgg                                                                                                    >  2:279408/1‑151 (MQ=255)                                           tttATGGTCCGCACGTCACCAATCAGATTATCCGCGAAGCGCTTTATCCTTTCTCTTACGACCTGACTATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCgg                                                                                                    <  1:196364/150‑1 (MQ=255)                                            ttATGGTCCGCACGTCACCAATCAGATTATCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTg                                                                                                  >  1:47122/1‑151 (MQ=255)                                             tATGGTCCGCACGTCACCAATCAGATTATCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTg                                                                                                  <  2:775717/150‑1 (MQ=255)                                              aTGGTCCGCACGTCACCAATCAGATTATCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCa                                                                                                <  2:210882/151‑1 (MQ=255)                                               tGGTCCGCACGTCACCAATCAGATTATCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCAc                                                                                               >  2:175727/1‑151 (MQ=255)                                                     gCACGTCACCAATCAGATTATCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAAt                                                                                         <  1:672015/151‑1 (MQ=255)                                                       aCGTCACCAATCAGATTATCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCt                                                                                       >  1:267458/1‑151 (MQ=255)                                                       aCGTCACCAATCAGATTATCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCt                                                                                       <  2:76832/151‑1 (MQ=255)                                                            aCCAATCAGATTATCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACGTa                                                                                  <  1:103157/151‑1 (MQ=255)                                                                       tATCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACGTAATCTCGGGCTg                                                                       >  1:247475/1‑151 (MQ=255)                                                                        aTCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACGTAATCTCGGGCTg                                                                       <  1:882224/150‑1 (MQ=255)                                                                          ccGCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACGTAATCTCGGGCTGGAc                                                                    <  2:718883/151‑1 (MQ=255)                                                                           cgcgAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACGTAATCTCGGGCTGGACg                                                                   <  2:173371/151‑1 (MQ=255)                                                                                aGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACGTAATCTCGGGCTGGACGTGCTg                                                              >  2:211933/1‑151 (MQ=255)                                                                                       tATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACGTAATCTCGGGCTGGACGTGCTGGATGTgg                                                       >  1:93577/1‑151 (MQ=255)                                                                                          ccTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACGTAATCTCGGGCTGGACGTGCTGGATGTGGTTa                                                    <  2:429396/151‑1 (MQ=255)                                                                                              aCTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACGTAATCTCGGGCTGGACGTGCTGGATGTGGTTAACCt                                                >  2:746173/1‑151 (MQ=255)                                                                                                   gacgacCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACGTAATCTCGGGCTGGACGTGCTGGATGTGGTTAACCTGcgcg                                           <  2:162763/151‑1 (MQ=255)                                                                                                           gACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACGTAATCTCGGGCTGGACGTGCTGGATGTGGTTAACCTGCGCGTTatgatg                                   >  2:745129/1‑151 (MQ=255)                                                                                                             cAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACGTAATCTCGGGCTGGACGTGCTGGATGTGGTTAACCTGCGCGTTATGATggg                                 >  2:39432/1‑151 (MQ=255)                                                                                                             cAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACGTAATCTCGGGCTGGACGTGCTGGATGTGGTTAACCTGCGCGTTATGATggg                                 <  1:348318/151‑1 (MQ=255)                                                                                                             cAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACGTAATCTCGGGCTGGACGTGCTGGATGTGGTTAACCTGCGCGTTATGATggg                                 >  2:100692/1‑151 (MQ=255)                                                                                                             cAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACGTAATCTCGGGCTGGACGTGCTGGATGTGGTTAACCTGCGCGTTATGATggg                                 <  1:790158/151‑1 (MQ=255)                                                                                                                 tGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACGTAATCTCGGGCTGGACGTGCTGGATGTGGTTAACCTGCGCGTTATGATGGGGGAt                             >  1:126233/1‑151 (MQ=255)                                                                                                                   tCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACGTAATCTCGGGCTGGACGTGCTGGATGTGGTTAACCTGCGCGTTATGATGGGGGATgg                           >  2:373559/1‑151 (MQ=255)                                                                                                                        aaaaTTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACGTAATCTCGGGCTGGACGTGCTGGATGTGGTTAACCTGCGCGTTATGATGGGGGATGGTCAt                       >  1:35561/1‑150 (MQ=255)                                                                                                                        aaaaTTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACGTAATCTCGGGCTGGACGTGCTGGATGTGGTTAACCTGCGCGTTATGATGGGGGATGGTCATg                      <  1:211959/151‑1 (MQ=255)                                                                                                                           aTTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACGTAATCTCGGGCTGGACGTGCTGGATGTGGTTAACCTGCGCGTTATGATGGGGGATGGTCATGGcc                   >  2:565368/1‑151 (MQ=255)                                                                                                                              ggTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACGTAATCTCGGGCTGGACGTGCTGGATGTGGTTAACCTGCGCGTTATGATGGGGGATGGTCATGGCCCa                 <  1:604231/150‑1 (MQ=255)                                                                                                                                   gcggcgTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACGTAATCTCGGGCTGGACGTGCTGGATGTGGTTAACCTGCGCGTTATGATGGGGGATGGTCATGGCCCAGCGGa            >  2:63649/1‑150 (MQ=255)                                                                                                                                        gTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACGTAATCTCGGGCTGGACGTGCTGGATGTGGTTAACCTGCGCGTTATGATGGGGGATGGTCATGGCCCAGCGGAAGGATc      >  1:232168/1‑151 (MQ=255)                                                                                                                                        gTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACGTAATCTCGGGCTGGACGTGCTGGATGTGGTTAACCTGCGCGTTATGATGGGGGATGGTCATGGCCCAGCGGAAGGATc      <  2:729085/151‑1 (MQ=255)                                                                                                                                           gagaGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACGTAATCTCGGGCTGGACGTGCTGGATGTGGTTAACCTGCGCGTTATGATGGGGGATGGTCATGGCCCAGCGGAAGGATCGAt   >  2:361708/1‑151 (MQ=255)                                                                                                                                            agagGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACGTAATCTCGGGCTGGACGTGCTGGATGTGGTTAACCTGCGCGTTATGATGGGGGATGGTCATGGCCCAGCGGAAGGATCGAtt  <  1:352108/151‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                  AGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCACGTCACCAATCAGATTATCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGACGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACGTAATCTCGGGCTGGACGTGCTGGATGTGGTTAACCTGCGCGTTATGATGGGGGATGGTCATGGCCCAGCGGAAGGATCGATT  >  NC_000913/1474351‑1474640

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑