Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,582,048	A→G	G159G (GGT→GGC)	ydeN ←	putative Ser‑type periplasmic non‑aryl sulfatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,582,048	0	A	G	100.0%	195.8 / NA	53	G159G (GGT→GGC)	ydeN	putative Ser‑type periplasmic non‑aryl sulfatase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/23);  total (30/23)

GTGAAGTTGTCATGATAGTCACGCGTTTGTTTATCTTCCGGTACCGGCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCATCCATTAATGAAAGGA  >  NC_000913/1581902‑1582190                                                                                                                                                   |                                                                                                                                               gtgAAGTTGTCATGATAGTCACGCGTTTGTTTATCTTCCGGTACCGGCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCAt                                                                                                                                            <  2:304437/151‑1 (MQ=255) gtgAAGTTGTCATGATAGTCACGCGTTTGTTTATCTTCCGGTACCGGCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCAt                                                                                                                                            <  2:580535/151‑1 (MQ=255)        tGTCATGATAGTCACGCGTTTGTTTATCTTCCGGTACCGGCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGc                                                                                                                                     >  2:799777/1‑151 (MQ=255)               aTAGTCACGCGTTTGTTTATCTTCCGGTACCGGCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTa                                                                                                                              <  1:538166/151‑1 (MQ=255)                   tCACGCGTTTGTTTATCTTCCGGTACCGGCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTgg                                                                                                                          >  1:381222/1‑151 (MQ=255)                    cACGCGTTTGTTTATCTTCCGGTACCGGCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTgg                                                                                                                          <  2:35394/150‑1 (MQ=255)                       gcgTTTGTTTATCTTCCGGTACCGGCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAAt                                                                                                                       >  1:890330/1‑150 (MQ=255)                             gtttATCTTCCGGTACCGGCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACAcc                                                                                                                <  1:547239/151‑1 (MQ=255)                               ttATCTTCCGGTACCGGCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCa                                                                                                               <  1:632848/150‑1 (MQ=255)                                   cTTCCGGTACCGGCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAgcg                                                                                                          >  2:820551/1‑151 (MQ=255)                                     tCCGGTACCGGCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAg                                                                                                            <  2:381196/147‑1 (MQ=255)                                      ccGGTACCGGCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAgcgggc                                                                                                       <  2:176959/151‑1 (MQ=255)                                        ggTACCGGCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAgcgggcgg                                                                                                     >  2:889610/1‑151 (MQ=255)                                         gTACCGGCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGcgggcggg                                                                                                    >  2:617598/1‑151 (MQ=255)                                            ccGGCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGc                                                                                                 <  2:223727/151‑1 (MQ=255)                                             cGGCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGa                                                                                                   >  1:831483/1‑148 (MQ=255)                                               gCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCg                                                                                              <  2:881512/151‑1 (MQ=255)                                               gCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCg                                                                                              >  1:846661/1‑151 (MQ=255)                                                cacaTTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGa                                                                                             <  2:818044/151‑1 (MQ=255)                                                   aTTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCg                                                                                          <  1:781970/151‑1 (MQ=255)                                                         gATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCatt                                                                                    <  1:279876/151‑1 (MQ=255)                                                              ttGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTgc                                                                               >  2:552450/1‑151 (MQ=255)                                                              ttGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTg                                                                                <  2:385899/150‑1 (MQ=255)                                                               tGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTgc                                                                               <  1:257971/150‑1 (MQ=255)                                                                 aCAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTgcggc                                                                            >  1:854788/1‑151 (MQ=255)                                                                    aGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGcgcg                                                                         >  1:739480/1‑151 (MQ=255)                                                                     gTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCgg                                                                        >  2:904209/1‑151 (MQ=255)                                                                      tGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCggg                                                                       >  2:221564/1‑151 (MQ=255)                                                                          ttttACGTACTGCTGTAGTGCAATAACCATGATTCTGGAATAATTTATGGAAGTAAGTTTTTGTTGGTGTAATGCCAACCGGGGAATCGGTAATGGAATAGAAACCAAAGCGGACGGGAGCTCGACCGGTCATTATTgcg                                                                              >  2:590378/2‑140 (MQ=255)                                                                                   ctgctgCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGaaa                                                           <  1:503763/150‑1 (MQ=255)                                                                                            tgtAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGc                                                 >  1:657131/1‑151 (MQ=255)                                                                                              taataaCCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGcc                                                >  1:456971/1‑150 (MQ=255)                                                                                                ataaCCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCcac                                              >  1:145421/1‑150 (MQ=255)                                                                                                   aCCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACAt                                            <  2:660046/149‑1 (MQ=255)                                                                                                      aTGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCg                                       >  1:461572/1‑151 (MQ=255)                                                                                                         aTTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGtt                                     >  1:483888/1‑150 (MQ=255)                                                                                                           tCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAg                                   <  2:41984/150‑1 (MQ=255)                                                                                                             tGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGATTGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTa                                 >  1:95829/1‑150 (MQ=255)                                                                                                                    atTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTAc                         >  2:299549/1‑151 (MQ=255)                                                                                                                    atTCAGGCAAGAAAGTTTCTGTTAGCGGAATGACATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTAc                         >  1:51821/1‑151 (MQ=255)                                                                                                                       cAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGATCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGc                       <  1:858676/150‑1 (MQ=255)                                                                                                                          gCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGTCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTc                   <  2:200033/151‑1 (MQ=255)                                                                                                                          gCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTc                   <  1:436846/151‑1 (MQ=255)                                                                                                                            aaGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCAt                 >  1:203443/1‑151 (MQ=255)                                                                                                                               aaaGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCATcc               >  2:335141/1‑150 (MQ=255)                                                                                                                               aaaGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCATcc               >  1:446157/1‑150 (MQ=255)                                                                                                                               aaaGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCATcc               >  1:898185/1‑150 (MQ=255)                                                                                                                                  gTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCATCCAtt            >  2:703345/1‑150 (MQ=255)                                                                                                                                   tttCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCATCCATTa           >  1:578823/1‑150 (MQ=255)                                                                                                                                        gTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCATCCATTAATGaa      >  1:503886/1‑150 (MQ=255)                                                                                                                                           aGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCATCCATTAATGAAAGGa  <  1:687665/151‑1 (MQ=255)                                                                                                                                              ggAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGt                                  >  1:738580/1‑116 (MQ=255)                                                                                                                                              ggAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGt                                  <  2:738530/116‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                               GTGAAGTTGTCATGATAGTCACGCGTTTGTTTATCTTCCGGTACCGGCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCATCCATTAATGAAAGGA  >  NC_000913/1581902‑1582190

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑