Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,604,698	A→G	T218A (ACG→GCG)	lsrD →	autoinducer 2 import system permease protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,604,698	0	A	G	100.0%	210.3 / NA	59	T218A (ACG→GCG)	lsrD	autoinducer 2 import system permease protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (37/22);  total (37/22)

TATCTTCCTGATATGTCTCCTCGTTTTCTGGCTCTGGCTGCATAAAACCCATGCCGGACGTAATGTGTTTTTGATTGGGCAAAGCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGACGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTT  >  NC_000913/1604550‑1604839                                                                                                                                                     |                                                                                                                                              tATCTTCCTGATATGTCTCCTCGTTTTCTGGCTCTGGCTGCATAAAACCCATGCCGGACGTAATGTGTTTTTGATTGGGCAAAGCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGGCg                                                                                                                                             >  2:453480/1‑151 (MQ=255) tATCTTCCTGATATGTCTCCTCGTTTTCTGGCTCTGGCTGCATAAAACCCATGCCGGACGTAATGTGTTTTTGATTGGGCAAAGCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGGCg                                                                                                                                             >  1:623569/1‑151 (MQ=255)  aTCTTCCTGATATGTCTCCTCGTTTTCTGGCTCTGGCTGCATAAAACCCATGCCGGACGTAATGTGTTTTTGATTGGGCAAAGCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGGCgg                                                                                                                                            >  1:340997/1‑151 (MQ=255)   tCTTCCTGATATGTCTCCTCGTTTTCTGGCTCTGGCTGCATAAAACCCATGCCGGACGTAATGTGTTTTTGATTGGGCAAAGCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGGCggg                                                                                                                                           >  2:239211/1‑151 (MQ=255)    cTTCCTGATATGTCTCCTCGTTTTCTGGCTCTGGCTGCATAAAACCCATGCCGGACGTAATGTGTTTTTGATTGGGCAAAGCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGGCgggg                                                                                                                                          >  1:9099/1‑151 (MQ=255)     ttCCTGATATGTCTCCTCGTTTTCTGGCTCTGGCTGCATAAAACCCATGCCGGACGTAATGTGTTTTTGATTGGGCAAAGCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGGCgggg                                                                                                                                          >  2:56413/1‑150 (MQ=255)          gATATGTCTCCTCGTTTTCTGGCTCTGGCTGCATAAAACCCATGCCGGACGTAATGTGTTTTTGATTGGGCAAAGCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGGCGGGGCTGGCg                                                                                                                                    >  1:85146/1‑151 (MQ=255)            tatGTCTCCTCGTTTTCTGGCTCTGGCTGCATAAAACCCATGCCGGACGTAATGTGTTTTTGATTGGGCAAAGCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGGCGGGGCTGGCGt                                                                                                                                   >  1:854347/1‑150 (MQ=255)            tatGTCTCCTCGTTTTCTGGCTCTGGCTGCATAAAACCCATGCCGGACGTAATGTGTTTTTGATTGGGCAAAGCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGGCGGGGCTGGCGt                                                                                                                                   >  2:477102/1‑150 (MQ=255)                tctcCTCGTTTTCTGGCTCTGGCTGCATAAAACCCATGCCGGACGTAATGTGTTTTTGATTGGGCAAAGCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGGCGGGGCTGGCGTCTGCg                                                                                                                              >  1:468359/1‑151 (MQ=255)                 ctcctcGTTTTCTGGCTCTGGCTGCATAAAACCCATGCCGGACGTAATGTGTTTTTGATTGGGCAAAGCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGGCGGGGCTGGGGTCTGCgg                                                                                                                             >  2:651212/1‑151 (MQ=255)                 ctcctcGTTTTCTGGCTCTGGCTGCATAAAACCCATGCCGGACGTAATGTGTTTTTGATTGGGCAAAGCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGGCGGGGCTGGCGTCTGCgg                                                                                                                             >  1:224927/1‑151 (MQ=255)                     tcGTTTTCTGGCTCTGGCTGCATAAAACCCATGCCGGACGTAATGTGTTTTTGATTGGGCAAAGCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGGCGGGGCTGGCGTCTGCGGTcgc                                                                                                                         >  2:193658/1‑151 (MQ=255)                        ttttCTGGCTCTGGCTGCATAAAACCCATGCCGGACGTAATGTGTTTTTGATTGGGCAAAGCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGGCGGGGCTGGCGTCTGCGGTcgccg                                                                                                                       >  1:618446/1‑150 (MQ=255)                          ttCTGGCTCTGGCTGCATAAAACCCATGCCGGACGTAATGTGTTTTTGATTGGGCAAAGCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGGCGGGGCTGGCGTCTGCGGTCGCCGCt                                                                                                                     >  2:665941/1‑150 (MQ=255)                                ctctggctGCATAAAACCCATGCCGGACGTAATGTGTTTTTGATTGGGCAAAGCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGGCGGGGCTGGCGTCTGCGGTCGCCGCtgtg                                                                                                                  >  2:63572/1‑147 (MQ=255)                                ctctggctGCATAAAACCCATGCCGGACGTAATGTGTTTTTGATTGGGCAAAGCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGGCGGGGCTGGCGTCTGCGGTCGCCGCTGtgctg                                                                                                               >  2:728380/1‑150 (MQ=255)                                    ggctGCATAAAACCCATGCCGGACGTAATGTGTTTTTGATTGGGCAAAGCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGGCGGGGCTGGCGTCTGCGGTCGCCGCTGTgctgct                                                                                                             <  1:174168/148‑1 (MQ=255)                                    ggctGCATAAAACCCATGCCGGACGTAATGTGTTTTTGATTGGGCAAAGCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGGCGGGGCTGGCGTCTGCGGTCGCCGCTGTGctgctg                                                                                                            <  2:21075/149‑1 (MQ=255)                                          aTAAAACCCATGCCGGACGTAATGTGTTTTTGATTGGGCAAAGCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGGCGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGt                                                                                                     >  2:219945/1‑150 (MQ=255)                                          aTAAAACCCATGCCGGACGTAATGTGTTTTTGATTGGGCAAAGCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGGCGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTa                                                                                                    <  2:860824/151‑1 (MQ=255)                                              aaCCCATGCCGGACGTAATGTGTTTTTGATTGGGCAAAGCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGGCGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTAtttt                                                                                                <  1:269183/151‑1 (MQ=255)                                                  cATGCCGGACGTAATGTGTTTTTGATTGGGCAAAGCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGGCGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGtt                                                                                            <  2:546957/151‑1 (MQ=255)                                                   aTGCCGGACGTAATGTGTTTTTGATTGGGCAAAGCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGGCGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTc                                                                                           <  1:278399/151‑1 (MQ=255)                                                     gCCGGACGTAATGTGTTTTTGATTGGGCAAAGCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGGCGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCa                                                                                          <  2:263921/150‑1 (MQ=255)                                                     gCCGGACGTAATGTGTTTTTGATTGGGCAAAGCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGGCGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAg                                                                                         <  2:733477/151‑1 (MQ=255)                                                     gCCGGACGTAATGTGTTTTTGATTGGGCAAAGCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGGCGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAg                                                                                         <  2:897182/151‑1 (MQ=255)                                                     gCCGGACGTAATGTGTTTTTGATTGGGCAAAGCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGGCGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAg                                                                                         <  1:221715/151‑1 (MQ=255)                                                        ggACGTAATGTGTTTTTGATTGGGCAAAGCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGGCGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCAc                                                                                      >  1:865969/1‑151 (MQ=255)                                                         gACGTAATGTGTTTTTGATTGGGCAAAGCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGGCGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACg                                                                                     >  1:351474/1‑151 (MQ=255)                                                         gACGTAATGTGTTTTTGATTGGGCAAAGCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGGCGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACg                                                                                     <  1:864228/151‑1 (MQ=255)                                                            gTAATGTGTTTTTGATTGGGCAAAGCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGGCGGGGCTGGCGTCTGCGGGCGCCGCTGTGCTGCTGGGATCGTATTTTGGTTCAGCACGTTc                                                                                  >  1:412678/1‑151 (MQ=255)                                                              aaTGTGTTTTTGATTGGGCAAAGCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGGCGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTcc                                                                                 <  1:56435/150‑1 (MQ=255)                                                                 gtgtTTTTGATTGGGCAAAGCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGGCGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCg                                                                                >  1:673107/1‑148 (MQ=255)                                                                    tttttGATTGGGCAAAGCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGGCGGGGCTGGCGTCTGCGGTCGCCGCTGGGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGAtctc                                                                           >  2:142061/1‑150 (MQ=255)                                                                                cAAAGCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGGCGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGGGCGTCGttt                                                               >  2:258247/1‑150 (MQ=255)                                                                                cAAAGCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGGCGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGGATCGTATTTTGGTTCAGCCCGTTCCGATCTCGGTGCGTCGttt                                                               >  2:216758/1‑150 (MQ=255)                                                                                   aGCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGGCGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGa                                                           <  2:778482/151‑1 (MQ=255)                                                                                    gCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGGCGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTTGTTCAGCACGTTCCGATCTCGGTGCGGCGTTTCTGAt                                                          >  2:730432/1‑151 (MQ=255)                                                                                                 gcTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGGCGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCAcc                                             >  1:97429/1‑151 (MQ=255)                                                                                                   tttATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGGCGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCCCGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATTAcc                                             >  2:511972/1‑149 (MQ=255)                                                                                                     tataGCGCGATTCCAGTTAACCGTACCTTATGCGCGCTCTATGCCATGGCGGGGCTGGCGTCTGCGGTCGCCGCTGGGCTGCTGGTATCCTACTTTGGTTCAGCACGTTCCGATCCCGGCGCGGCGTTTccggatacc                                                      >  1:580430/1‑130 (MQ=255)                                                                                                               ttCCATTTAACCGTACCTTATGTGCGCTCTATGCCATGGCGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCtt                                 <  2:749077/149‑1 (MQ=255)                                                                                                               ttCCAGTTACCCGTCCCTTATGTGCGCTCTATGCCATGGCGGGGCTGGCTTCTGCGGTCGCCGCTGTGCTGCTGGTTTCGTATTTTGGTTCAGCCCGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTg                                <  2:759082/150‑1 (MQ=255)                                                                                                               ttCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGGCGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTgg                               >  2:278656/1‑151 (MQ=255)                                                                                                               ttCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGGCGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTg                                <  2:747390/150‑1 (MQ=255)                                                                                                                  cAGTTAACCGTACCTTATGTGCGCTCTATGCCATGGCGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCgg                            <  1:127735/151‑1 (MQ=255)                                                                                                                   aGTTAACCGTACCTTATGTGCGCTCTATGCCATGGCGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCggg                           >  1:60695/1‑151 (MQ=255)                                                                                                                      tAACCGTACCTTATGTGCGCTCTATGCCATGGCGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGcc                        >  2:430971/1‑151 (MQ=255)                                                                                                                       acccGTACTTTATGTGCGTTTTATGCCAGGGCGGGGCTGGCGTCTGCGTTCGCCGCTGTGCTGCTGTTATCGTATTTTGGTTCAGCACGTTCCGATCTGGGTGGGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGc                         <  2:5565/147‑1 (MQ=255)                                                                                                                            tACCTTGTTGGCGCTCGATGCCATGGCGGGGCGGGCGTCTGGGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTCCACCCCGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAatat                   <  2:608415/150‑1 (MQ=255)                                                                                                                                ttATGTGCGCTCTATGCCATGGCGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTAt               >  1:10851/1‑150 (MQ=255)                                                                                                                                 tATGTGCGCTCTATGCCATGGCGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTAtgg             >  1:827687/1‑151 (MQ=255)                                                                                                                                     tgCGCTCTATGCCATGGCGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGGATCGTATTTTTGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCCTCACCGCCGGGGTGCTTGGCGGGGCCAATATTTTTggtggt         >  2:756305/1‑151 (MQ=255)                                                                                                                                       cgcTCTATGCCATGGCGGGGCTGGCGTCTGCGGCCGCCGCTGTGCTCCGGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTggtt        <  1:138037/150‑1 (MQ=255)                                                                                                                                          tctATGCCATGGCGGGGCTGGCCTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTTTTTTGGTTCAGCACGTTCCTATCTCGGTGCGTCTTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGTCAATATTTATGGTggttcc      <  1:863694/149‑1 (MQ=255)                                                                                                                                            tATGCAATGGCGGGGCTGGCGTCTGGGGTGGCCGCTGTGCGTCTGTTATCGTTTTTTGGTTCAGCCCGTTCCGATCTGGGGGGGGCTTTTCTGAGGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTggttccg     <  2:483050/148‑1 (MQ=255)                                                                                                                                              tGCCATGGCGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGGGCTTGGCGGGGCCCATATTTATGGTgggtccgggt  >  2:135828/1‑147 (MQ=255)                                                                                                                                              tGCCATGGCGGGGCTGGCGTCCGCGGGCGCCGCTGTGCTGCTGGGATCGGATTTTGGGTCAGCACGGTCCGATCTCGGGGCGCCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGGCCATATTTTTGGTggtttcgg    >  2:22500/1‑147 (MQ=255)                                                                                                                                                     |                                                                                                                                              TATCTTCCTGATATGTCTCCTCGTTTTCTGGCTCTGGCTGCATAAAACCCATGCCGGACGTAATGTGTTTTTGATTGGGCAAAGCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGACGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTT  >  NC_000913/1604550‑1604839

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑