Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,812,7920CA42.5% 70.6 / 22.9 47T21N (ACC→AAC) dfpfused 4'‑phosphopantothenoylcysteine decarboxylase/phosphopantothenoylcysteine synthetase, FMN‑binding
Reads supporting (aligned to +/- strand):  ref base C (7/20);  new base A (20/0);  total (27/20)
Fisher's exact test for biased strand distribution p-value = 9.79e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01

CAAAGTGAATCTCAGGTGAAGGCACGACAACAAAAGTATTGTGATAAAATCGCCAACTTCTGGTGTCACCCTACAGGAAAAATCATCATGAGCCTGGCCGGTAAAAAAATCGTTCTCGGCGTTAGCGGCGGTATTGCTGCCTAT‑AAAACCCCTGAACTGGTGCGTCGTTTGCGCGATCGCGGGGCCGACGTCCGCGTAGCCATGACCGAAGCGGCAAAAGCCTTTATCACCCCACTTAGCTTG‑CAGGCGGTTTCTGGTTATCCCGTTTCCGACAGTCTGCTGGACCCGGCAGCCG  >  NC_000913/3812644‑3812938
                                                                                                                                                     |                                                                                                                                                   
cAAAGTGAATCTCAGGTGAAGGCACGACAACAAAAGTATTGTGATAAAATCGCCAACTTCTGGTGTCACCCTACAGGAAAAATCATCATGAGCCTGGCCGGTAAAAAAATCGTTCTCGGCGTTAGCGGCGGTATTTCTGCCTAT‑AAAAacc                                                                                                                                                   >  1:585893/1‑151 (MQ=255)
     tGAATCTCAGGTGAAGGCACGACAACAAAAGTATTGTGATAAAATCGCCAACTTCTGGTGTCACCCTACAGGAAAAATCATCATGAGCCTGGCCGGTAAAAAAATCGTTCTCGGCGTTAGCGGCGGTATTGCTGCCTAT‑AAAAACCCCGa                                                                                                                                               >  1:89380/1‑150 (MQ=255)
            cAGGTGAAGGCACGACAACAAAAGTATTGTGATAAAATCGCCAACTTCTGGTGTCACCCTACAGGAAAAATCATCATGAGCCTGGCCGGTAAAAAAATCGTTCTCGGCGTTAGCGGCGGTATTTCTGCCCAT‑AAAAACCCCGAACTgg                                                                                                                                          >  1:289129/1‑148 (MQ=255)
              ggTGAAGGCACGACAACAAAAGTATTGTGATAAAATCGCCAACTTCTGGTGTCACCCTACAGGAAAAATCATCATGAGACTGGCCGGTAAAAAAATCGTTCTCGGCGTTAGCGGCGGTATTGCTGCCTAT‑AAAACCCCTGAACTGGTGcgt                                                                                                                                     <  1:445813/151‑1 (MQ=255)
                      cACGACAACAAAAGTATTGTGATAAAATCGCCAACTTCTGGTGTCACCCTACAGGAAAAATCATCATGAGCCTGGCCGGTAAAAAAATCGTTCTCGGCGTTAGCGGCGGTATTGCTGCCTAT‑AAAAACCCCGAACTGGTGGGTCGttt                                                                                                                                >  1:520413/1‑148 (MQ=255)
                           caacaaAAGTATTGTGATAAAATCGCCAACTTCTGGTGTCACCCTACAGGAAAAATCATCATGAGCCTGGCCGGTAAAAAAATCGTTCTCGGCGTTAGCGGCGGTGTTGCTCCCCAT‑TAAACCCCTCAAATTGTGGGTCGCTTgcgc                                                                                                                            >  1:470688/1‑147 (MQ=255)
                                  aGTATTGTGATAAAATCGCCAACTTCTGGTGTCACCCTACAGGAAAAATCATCATGAGCCTGGCCGGTAAAAAAATCGTTCTCGGCGTTAGCGGCGGTATTGCTGCCTAT‑AAAAAACCTGAAATGGTGGGTCGTTTGCGCGATCGCgggg                                                                                                                  >  1:633181/1‑150 (MQ=255)
                                   gTATTGTGATAAAATCGCCAACTTCTGGTGTCACCCTACAGGAAAAATCATCATGAGCCTGGCCGGTAAAAAAATCGTTCTCGGCGTTAGCGGCGGTATTGCTGCCTAT‑AAAACCCCTGAACTGGTGCGTCGTTTGCGCGATCGCGGGGcc                                                                                                                <  2:666592/151‑1 (MQ=255)
                                     aTTGTGATAAAATCGCCAACTTCTGGTGTCACCCTACAGGAAAAATCATCATGAGCCTGGCCGGTAAAAAAATCGTTCTCGGCGTTAGCGGCGGTATTTCTGCCCAT‑AAAAACCCCGAAAAGGGGCGTCGTTTTCGCGCTAGCGGGGcc                                                                                                                >  2:262238/1‑149 (MQ=255)
                                         tgATAAAATCGCCAACTTCTGGTGTCACCCTACAGGAAAAATCATCATGAGCCTGGCCGGTAAAAAAATCGTTCTCGGCGTTAGCGGCGGCATTGGTGCCTAT‑AAAAACCCCCAACAGGTGCGTCGTTTTGGCGAGCGCGGGGCCgcc                                                                                                             >  2:61581/1‑146 (MQ=255)
                                          gATAAAATCGCCAACTTCTGGTGTCACCCTACAGGAAAAATCATCATGAGCCTGGCCGGTAAAAAAATCGTTCTCGGCGTTAGCGGCGGTATTGCTGCCTAT‑AAAACCCCTGAACTGGTGCGTCGTTTGCGCGATCGCGGGGCCGACGTc                                                                                                          <  1:419012/150‑1 (MQ=255)
                                           aTAAAATCGCCAACTTCTGGTGTCACCCTACAGGAAAAATCATCATGAGCCTGGCCGGTAAAAAAATCGTTCTCGGCGTTAGCGGCGGTATTGCTGCCTAT‑AAAAACCCTGAACTTGGGCGTCGGTTGCGGGATCGCGGGGCCGACGTcc                                                                                                         >  1:61210/1‑150 (MQ=255)
                                             aaaaTCGCCAACTTCTGGTGTCACCCTACAGGAAAAATCATCATGAGCCTGGCCGGTAAAAAAATCGTTCTCGGCGTTAGCGGCGGTATTTCTGCCTAT‑AAAAACCCTGAACTGGTGCGTCGTTTGCGCGATCGCGGGGGCCACGGCcgc                                                                                                       >  1:31995/1‑150 (MQ=255)
                                              aaaTCGCCAACTTCTGGTGTCACCCTACAGGAAAAATCATCATGAGCCTGGCCGGTAAAAAAATCGTTCTCGGCGTTAGCGGCGGGATTGCTTCCCAT‑AAAAACCCCGAACTTGGGGGTCGGTTTCGCGGGTGCGGGGCCGGCCTCcacg                                                                                                      >  2:516190/1‑150 (MQ=255)
                                                  cGCCAACTTCTGGTGTCACCCTACAGGAAAAATCATCATGAGCCTGGCCGGTAAAAAAATCGTTCTCGGCGTTAGCGGCGGTATTGCTGCCTCT‑AAAACCCCTCCACTTGGGCGTCGTTTGCGCGCTCGCGGGGGCGGCGGCCCCGTAGcc                                                                                                 >  2:616223/1‑151 (MQ=255)
                                                     cAACTTCTGGTGTCACCCTACAGGAAAAATCATCATGAGCCTGGCCGGTAAAAAAATCGTTCTCGGCGTTAGCGGCGGGATTTCTGCCTAT‑AAAAACCCCGAAATGGTGCGTCGTTTGCGCGAGCGCGGGGGCGACGGCCGCGTAGCCATg                                                                                              >  1:297582/1‑151 (MQ=255)
                                                      aaCTTCTGGTGTCACCCTACAGGAAAAATCATCATGAGCCTGGCCGGTAAAAAAATCGTTCTCGGCGTTAGCGGCGGTATTGCTGCCTAT‑AAAACCCCTGAACTGGTGCGTCGTTTGCGCGATCGCGGGGCCGACGTCCGCGTAGCCATGa                                                                                             <  1:348968/151‑1 (MQ=255)
                                                      aaCTTCTGGTGTCACCCTACAGGAAAAATCATCATGAGCCTGGCCGGTAAAAAAATCGTTCTCGGCGTTAGCGGCGGTATTGCTGCCTAT‑AAAACCCCTGAACTGGTGCGTCGTTTGCGCGATCGCGGGGCCGACGTCCGCGTAGCCATGa                                                                                             <  1:499024/151‑1 (MQ=255)
                                                        cTTCTGGTGTCACCCTACAGGAAAAATCATCATGAGCCTGGCCGGTAAAAAAATCGTTCTCGGCGTTAGCGGCGGTATTGCTGCCTAT‑AAAAACCCTGAACTGGTGCGTCGTTTGCGCGGTCGCGGGGGCGACGACCGCGTAGCCCTGAcc                                                                                           >  1:463721/1‑151 (MQ=255)
                                                            tGGTGTCACCCTACAGGAAAAATCATCATGAGCCTGGCCGGTAAAAAAATCGTTCTCGGCGTTAGCGGCGGTATTGCTGCCTCT‑AAAAACCCCGAAATGGGGCGTCGTTTGCGCGATCGCGGGGCCGACGGCCGCGTAGCCATGAcc                                                                                           >  2:630666/1‑147 (MQ=255)
                                                                  cACCCTACAGGAAAAATCATCATGAGCCTGGCCGGTAAAAAAATCGTTCTCGGCGTTAGCGGCGGTATTGCTGCCTAT‑AAAACCCCTGAACTGGTGCGTCGTTTGCGCGATCGCGGGGCCGACGTCCGCGTAGCCATGACCGAAGCGGCa                                                                                  <  2:152344/150‑1 (MQ=255)
                                                                  cACCCTACAGGAAAAATCATCATGAGCCTGGCCGGTAAAAAAATCGTTCTCGGCGTTAGCGGCGGTATTGCTGCCTAT‑AAAACCCCTGAACTGGTGCGTCGTTTGCGCGATCGCGGGGCCGACGTCCGCGTAGCCATGACCGAAGCGGCa                                                                                  <  1:256418/150‑1 (MQ=255)
                                                                  cACCCTACAGGAAAAATCATCATGAGCCTGGCCGGTAAAAAAATCGTTCTCGGCGTTAGCGGCGGAATTTCTTCCTAT‑AAAAACCCCGAACATGGGGGTCCGTTGCGCGATCGCGGGGCCGCCGGCCGCGGAGCCAAGaacccagcg                                                                                     >  2:603845/1‑139 (MQ=255)
                                                                    cccTACAGGAAAAATCATCATGAGCCTGGCCGGTAAAAAAATCGTTCTCGGCGTTAGCGGCGGTATTGCTGCCTAT‑AAAACCCCTGAACTGGTGCGTCGTTTGCGCGATCGCGGGGCCGACGTCCGCGTAGCCATGACCGAAGCGGCaaa                                                                                <  2:561146/150‑1 (MQ=255)
                                                                      cTACAGGAAAAATCATCATGAGCCTGGCCGGTAAAAAAATCGTTCTCGGCGTTAGCGGCGGTATTGCTGCCTAT‑AAAAACCCTGAAATGGGGCGGCGTTTGCGCGATCGCGGGGCCGACGGCCGCGTAGCCATGAACGAAGCGGCaaaa                                                                               >  1:528848/1‑149 (MQ=255)
                                                                        aCAGGAAAAATCATCATGAGCCTGGCCGGTAAAAAAATCGTTCTCGGCGTTAGCGGCGGTATTGCTGCCTAT‑AAAACCCCTGAACTGGTGCGTCGTTTGCGCGATCGCGGGGCCGACGTCCGCGTAGCCATGACCGAAGCGGCAAAAGc                                                                             <  2:301704/149‑1 (MQ=255)
                                                                        aCAGGAAAAATCATCATGAGCCTGGCCGGTAAAAAAATCGTTCTCGGCGTTAGCGGCGGTATTGCTGCCTAT‑AAAAAACCTGAACTGTTGCGGCGTTTTCGCGGTCGCGGGGGCGATGACCGCGCAACCATGTCCGAAACGGCacaaac                                                                             >  2:10705/1‑147 (MQ=255)
                                                                          aGGAAAAATCATCATGAGCCTGGCCGGTAAAAAAATCGTTCTCGGCGTTAGCGGCGGTATTGCTGCCTAT‑AAAACCCCTGAACTGGTGCGTCGTTTGCGCGATCGCGGGGCCGACGTCCGCGTAGCCATGACCGAAGCGGCAAAAGCCttt                                                                         <  1:240899/151‑1 (MQ=255)
                                                                                    atcatGAGCCTGGCCGGTAAAAAAATCGTTCTCGGCGTTAGCGGCGGTATTGCTGCCTAT‑AAAACCCCTGAACTGGTGCGTCGTTTGCGCGATCGCGGGGCCGACGTCCGCGTAGCCATGACCGAAGCGGCAAAAGCCTTTATCACCCCAc                                                               <  1:166897/151‑1 (MQ=255)
                                                                                      catGAGCCTGGCCGGTAAAAAAATCGTTCTCGGCGTTAGCGGCGGTATTTCTGCCTAT‑AAAAACCCTGAAATGGGGCGGCGTTTGCGCGGTCGCGGGGGCGACGTCCCCCTAGGCATGACCGAAACGGCAAAAACCCTTTTTACCCCAcct                                                             >  1:698493/1‑149 (MQ=255)
                                                                                        tGAGCCTGGCCGGTAAAAAAATCGTTCTCGGCGTTAGCGGCGGTATTGCTGCCTAT‑AAAACCCCTGAACTGGTGCGTCGTTTGCGCGATCGCGGGGCCGACGTCCGCGTAGCCATGACCGAAGCGGCAAAAGCCTTTATCACCCCACtt                                                             <  1:345842/149‑1 (MQ=255)
                                                                                                gCCGGTAAAAAAATCGTTCTCGGCGTTAGCGGCGGTATTGCTGCCTAT‑AAAACCCCTGAACTGGTGCGTCGTTTGCGCGATCGCGGGGCCGACGTCCGCGTAGCCATGACCGAAGCGGCAAAAGCCTTTATCACCCCACTTAGCTTG‑CAgg                                                  <  2:83887/151‑1 (MQ=255)
                                                                                                     tAAAAAAATCGTTCTCGGCGTTAGCGGCGGTATTGCTGCCCTT‑AAAAAACCCGAACTGGGGCGTCGTTTGCGCGGTCGCGGGGCCGACGTCCCCGCAGCCACGACCGAAGCGGCGAAAACCTTTTTCCCCCCCCCTTGCtttccggggg                                                >  2:220454/1‑141 (MQ=255)
                                                                                                      aaaaaaaTCGTTCTCGGCGTTAGCGGCGGTATTGCTGCCTAT‑AAAACCCCTGAACTGGTGCGTCGTTTGCGCGATCGCGGGGCCGACGTCCGCGTAGCCATGACCGAAGCGGCAAAAGCCTTTATCACCCCACTTAGCTTG‑CAGGCGGtt                                             <  1:363739/150‑1 (MQ=255)
                                                                                                      aaaaaaaTCGTTCTCGGCGTTAGCGGCGGTATTGCTGCCTAT‑AAAACCCCTGAACTGGTGCGTCGTTTGCGCGATCGCGGGGCCGACGTCCGCGTAGCCATGACCGAAGCGGCAAAAGCCTTTATCACCCCACTTAGCTTG‑CAGGCGGtt                                             <  2:432450/150‑1 (MQ=255)
                                                                                                       aaaaaaTCGTTCTCGGCGTTAGCGGCGGTCTTGCTTCCCAT‑AAAAACCCCGACATGGGGTGCCCTGTGCCCGAGCGCGGGGCCGCCGGGCGCGGAACCATCAACCCAGCGGCACAAAGCTTTATCCCCCCACCTGGCTTG‑CCCGCGGcttt                                           >  2:574030/1‑150 (MQ=255)
                                                                                                       aaaaaaTCGTTCTCGGCGTTAGCGGCGGTATTGCTGCCTAT‑AAAACCCCTGAACTGGTGCGTCGTTTGCGCGATCGCGGGGCCGACGTCCGCGTAGCCATGACCGAAGCGGCAAAAGCCTTTATCACCCCACTTAGCTTG‑CAGGCGGTTTc                                           <  2:643197/151‑1 (MQ=255)
                                                                                                        aaaaaTCGTTCTCGGCGTTAGCGGCGGTATTTCTGCCTAT‑AAAAACCCCGAACTGGGGGGTCGTTTGCGCGATCGCGGGGCCGACGTCCGCGTAGCCATGACCGAAGCGGCAAAAACCTTTATCACCCCCCTTAGCTTT‑CAGGCGGgtt                                            >  2:320726/1‑149 (MQ=255)
                                                                                                        aaaaaTCGTTCTCGGCGTTAGCGGCGGTATTGCTGCCTAT‑AAAACCCCTGAACTGGTGCGTCGTTTGCGCGATCGCGGGGCCGACGTCCGCGTAGCCATGACCGAAGCGGCAAAAGCCTTTATCACCCCACTTAGCTTG‑CAGGCGGTTTCt                                          <  1:588242/151‑1 (MQ=255)
                                                                                                           aaTCGTTCTCGGCGTTAGCGGCGGTATTGGTGCCTATAAAAACCCCTG‑ACTGGTTCGGCGGTTTCGCCGTCGGGGGGGCGGCGGCCGCGGAGCCAAGAACGAAGCGGCAAAAACCTTTATCACCCCACCTTGCTTT‑CCGGCGGttttttgg                                       >  1:78653/1‑146 (MQ=255)
                                                                                                            aTCGTTCTCGGCGTTAGCGGCGGTATTGCTGCCTAT‑AAAAACCCTGAAATGGTGCGTCGGTTTCGCGATCGCGGGGCCGACGTCCGCGGAGCCATGACCGAAGCGGCAAAAACCTTTTTCACCCCCCTTAGCTTGCCGGGGGGTTTTTGGt                                       >  1:584783/1‑151 (MQ=255)
                                                                                                                 tctcGGCGTTAGCGGCGGTATTGCTGCCTAT‑AAAACCCCTGAACTGGTGCGTCGTTTGCGCGATCGCGGGGCCGACGTCCGCGTAGCCATGACCGAAGCGGCAAAAGCCTTTATCACCCCACTTAGCTTG‑CAGGCGGTTTCTGGTTATcc                                  <  1:649373/150‑1 (MQ=255)
                                                                                                                            gcggcgGTATTGCTGCCTAT‑AAAACCCCTGAACTGGTGCGTCGTTTGCGCGATCGCGGGGCCGACGTCCGCGTAGCCATGACCGAAGCGGCAAAAGCCTTTATCACCCCACTTAGCTTG‑CAGGCGGTTTCTGGTTATCCCGTTTCCGACAg                      <  2:594610/151‑1 (MQ=255)
                                                                                                                              ggcggTATTGCTGCCTAT‑AAAACCCCTGAACTGGTGCGTCGTTTGCGCGATCGCGGGGCCGACGTCCGCGTAGCCATGACCGAAGCGGCAAAAGCCTTTATCACCCCACTTAGCTTG‑CAGGCGGTTTTTGGTTATCCCGTTTCCGACAGt                     >  2:240266/1‑150 (MQ=255)
                                                                                                                                         tgcCTAT‑AAAACCCCTGAACTGGTGCGTCGTTTGCGCGATCGCGGGGCCGACGTCCGCGTAGCCATGACCGAAGCGGCAAAAGCCTTTATCACCCCACTTAGCTTG‑CAGGCGGTTTCTGGGTATCCCGTTTCCGACAGTCTGCTGGAccc          >  1:510283/1‑150 (MQ=255)
                                                                                                                                           ccTAT‑AAAACCCCTGAACTGGTGCGTCGTTTGCGCGATCGCGGGGCCGACGTCCGCGTAGCCATGACCGAAGCGGCAAAAGCCTTTATCACCCCACTTAGCTTG‑CAGGCGGTTTCTGGTTATCCCGTTTCCGACAGTCTGCTGGACCCGGc       <  1:608717/151‑1 (MQ=255)
                                                                                                                                                 aaaaCCCCTGAACTGGTGCGTCGTTTGCGCGATCGCGGGGCCGACGTCCGCGTAGCCATGACCGAAGCGGCAAAAGCCTTTATCACCCCACTTAGCTTG‑CAGGCGGTTTCTGGTTATCCCGTTTTCCACAGTCTGCTGGCCCCGgccgcc   >  2:140073/1‑150 (MQ=255)
                                                                                                                                                  aaaCCCCTGAACTGGTGCGTCGTTTGCGCGATCGCGGGGCCGACGTCCGCGTAGCCATGACCGAAGCGGCAAAAGCCTTTATCACCCCACTTAGCTTG‑CAGGCGGTTTCTGGTTATCCCGTTTCCGACAGTCTGCTGGACCCGGCAGCCg  >  2:448945/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
CAAAGTGAATCTCAGGTGAAGGCACGACAACAAAAGTATTGTGATAAAATCGCCAACTTCTGGTGTCACCCTACAGGAAAAATCATCATGAGCCTGGCCGGTAAAAAAATCGTTCTCGGCGTTAGCGGCGGTATTGCTGCCTAT‑AAAACCCCTGAACTGGTGCGTCGTTTGCGCGATCGCGGGGCCGACGTCCGCGTAGCCATGACCGAAGCGGCAAAAGCCTTTATCACCCCACTTAGCTTG‑CAGGCGGTTTCTGGTTATCCCGTTTCCGACAGTCTGCTGGACCCGGCAGCCG  >  NC_000913/3812644‑3812938

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑