Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,555,1060CA45.0% 62.2 / 10.8 40N177K (AAC→AAA) uxuRfructuronate‑inducible hexuronate regulon transcriptional repressor; autorepressor
Reads supporting (aligned to +/- strand):  ref base C (7/15);  new base A (18/0);  total (25/15)
Fisher's exact test for biased strand distribution p-value = 5.93e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATTGCAACTGGAAGAGCGTGAACTGGCTTCCAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATGTGGATCCAGTTGCACA‑GCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGT  >  NC_000913/4554959‑4555255
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aTTGCAACTGGAAGAGCGTGAACTGGCTTCCAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAaaaaaa                                                                                                                                                      >  2:19496/1‑150 (MQ=255)
     aaCTGGAAGAGCGTGAACTGGCTTCCAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGGAAAAAAAcc                                                                                                                                                   >  1:44011/1‑148 (MQ=255)
     aaCTGGAAGAGCGTGAACTGGCTTCCAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAAt                                                                                                                                                <  1:217979/151‑1 (MQ=255)
         ggAAGAGCGTGAACTGGCTTCCAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAAtgtg                                                                                                                                             <  1:623624/150‑1 (MQ=255)
                 gTGAACTGGCTTCCAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGGAAAAAAACCAATGTGGGTccc                                                                                                                                       >  2:500557/1‑147 (MQ=255)
                             ccAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATGTGGATCCAGTTGCACA‑GCCATc                                                                                                                        <  2:487050/150‑1 (MQ=255)
                                  gcgcCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGGAAAAAAACCCAAGTGGGTCCCGTTGTCCA‑GCCATCTgg                                                                                                                     >  2:213642/1‑148 (MQ=255)
                                    gcCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGGAAAAAATCCAACGTGGAGGACGCTGCACA‑GCCATCTGGGTg                                                                                                                  >  2:234054/1‑149 (MQ=255)
                                                  aaaGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATGTGGATCCAGTTGCACA‑GCCATCTGGATGACAGCCTGTATCGCaa                                                                                                  <  1:723491/151‑1 (MQ=255)
                                                      cGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGGAAAAAATCCAATGTGGGTCCCGGTTCACA‑GCCATCTGGGTGGAAGCCCGTATCGCcaa                                                                                                 >  2:329257/1‑148 (MQ=255)
                                                                    tCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATGTGGATCCAGTTGCACA‑GCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGTTGGGCGATc                                                                                 <  1:688238/150‑1 (MQ=255)
                                                                       aTCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAAAGTGGATCCAGGTGCACA‑GCCATCTGGATGACAACCTGTATCGCAAAAAGTGGGTGGGCGGTca                                                                                >  1:671117/1‑148 (MQ=255)
                                                                         ctcGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAAAAATTCAATTTGGGTCCAGTTGCACA‑GCCATCTGGATGACAGCCTGTATCGCAAAGAGGGGGTGGGCGATCACaaa                                                                            >  1:727218/1‑150 (MQ=255)
                                                                         ctcGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAAAAAACCCAAGGGGGACCCGTTGCCCA‑GCCCTCTGGGTGACAGGCCGTATCGCAAAAAGAGGGTGGGCGATCACaaa                                                                            >  2:78108/1‑150 (MQ=255)
                                                                           cGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATGTGGATCCAGTTGCACA‑GCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGTTGGGCGATCACAAACa                                                                          <  2:406614/150‑1 (MQ=255)
                                                                           cGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATGTGGATCCAGTTGCACA‑GCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGTTGGGCGATCACAAACAg                                                                         <  1:29497/151‑1 (MQ=255)
                                                                           cGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAAAAAACCAAAGGGGGTCCAGTTGGACA‑GCCCACTGGATGACAGGCCGTATTGCAAAAAAGGGGTGGGGGGGCACaaa                                                                            >  2:507244/1‑148 (MQ=255)
                                                                                  gCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAAACCAAAGTGGGTCCCGTTGCACA‑GCCACCTGGGTGACAGCCCGTATCGCCAAAAGAGGGTGGGGGGGCCCAAACaaaacccc                                                                   >  1:4691/1‑143 (MQ=255)
                                                                                     gAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATGTGGATCCAGTTGCACA‑GCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTcgccg                                                               <  2:719926/151‑1 (MQ=255)
                                                                                            cGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATGTGGATCCAGTTGCACA‑GCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAAt                                                        <  1:366159/151‑1 (MQ=255)
                                                                                              cATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAAAAATCCCATGTGGATCCCGTTGCACA‑GCCATCTGGGTGACAGCCTGTATCGCAAAGAGGGGGTTGGGGATCACAAACAGAACCTCCCCGCGGTaa                                                         >  1:410459/1‑148 (MQ=255)
                                                                                               aTAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGGAAAAAATTAAATGTGGATACAGTTTTACA‑GCCATCTGGGTGATAGCCTGTATCTCAAAGAGGGGTTGGGCGAACAAAAAAAGATACTCGCCCCGTTAATc                                                       >  1:2817/1‑149 (MQ=255)
                                                                                               aTAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAAAAATCCAATGTGGGATCAGTTGCACA‑GCCCTCTGGATGACAGCCTGGATTGCAAAGAGTGGGTGGGGGGTCACAAAAAAAACCTCGCCGCGTTTAt                                                        >  2:429136/1‑148 (MQ=255)
                                                                                                 aaCAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAAAAATCCAAAGTGGATCCAGGTGCACA‑GCCCTCTTGGTGACAGCCCGTTTCGCAAAAAGGGGGTGGGGGGTCACAAAAAAAACCTCCCCCCGGTTATCaaa                                                    >  2:30079/1‑150 (MQ=255)
                                                                                                   cAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGGAAAAAATCCAATGTGGGTCCCGTTGCACA‑GCCACCTGGGTGACAGCCCGTATCGCAAAGAGGGGGTGGGCGGTCACAAAAAGAACCTCCCCGCGGTAATCaaaaa                                                  >  2:492926/1‑150 (MQ=255)
                                                                                                   cAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAAAAATCCAATGTGGGTCCAGGTGCACA‑GCCATCTGGATGACAGCCCGTATCGCAAAGAGGGGTTGGGGGATCACAAAAAAAACCTCGCCGCGGTAATCaaaaa                                                  >  1:216740/1‑150 (MQ=255)
                                                                                                    aGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAAACCAATGTGGGTCCAGGTGCACA‑GCCATCTGGATGACAGCCTGTATCGCAAAGAGGGGGTGGGGGGTCACAAACAGATCCTCGCCGCGTTAATCaaaaaa                                                 >  1:74452/1‑150 (MQ=255)
                                                                                                             gtggAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAAAAAGCCCATGCGGAAACCGGTGGACAGGCCTTCGGGA‑GACAGCCTCCATCGGAAAAAGGGGGTTGGGGCGCACAAACAGAGCCCCCCCGCGTTCATCaaaaaaaaa                                              >  1:376024/1‑141 (MQ=255)
                                                                                                              tggAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAAAAATTCACAGTGGGTACCGCTGCACC‑GCCATCTGGGTGACAGCCTGTATTGCCAAGAGGGGGTGGGCGGACACAAAAAGAACCCCCCCCCGCTTATCAAAAAAAAAGCCCg                                         >  1:30660/1‑148 (MQ=255)
                                                                                                              tggAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAAAAATCCAAAGGGGGTCCCGTTGGACA‑GCCACCTGGGTGAAAGCCCGTATCGCAAAGAGTGGGTTGGCGAACACAAACAGAACCCCGCCCCCTTAATCAAAAAAAATGccc                                          >  2:101096/1‑147 (MQ=255)
                                                                                                                gAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGGAAACCAAACAATGGGGGTCCCGGTTCGCC‑ACCCCCTCGGGGACCGCCTGTATCGCAAAGAGGGGGTGGGCGGTCAAAAAAAAAGCCCCGCCCTGGTAAATAAAAAAAATGCCCGAgc                                      >  2:43868/1‑149 (MQ=255)
                                                                                                                                ccTGGCAGTGGCGGGAAAACAATCCAATGTGGATCCAGTTGCACA‑GCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAAtgt                     <  1:326723/150‑1 (MQ=255)
                                                                                                                                  tGGCAGTGGCGGGAAAAAAATCCAATGTGGATCCAGTTGCACA‑GCCATCTGGGTGACAGCCTGTATCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTCGCCGCGGTAATCAAAAAAGAAGCCCGAGCGGCGAAGCTGGCAATGTgg                   >  1:480409/1‑150 (MQ=255)
                                                                                                                                         ggCGGGAAAACAATCCAATGTGGATCCAGTTGCACA‑GCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCt           >  2:212558/1‑151 (MQ=255)
                                                                                                                                            gggAAAACAATCCAATGTGGATCCAGTTGCACA‑GCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTgg         >  1:213905/1‑150 (MQ=255)
                                                                                                                                            gggAAAACAATCCAATGTGGATCCAGTTGCACA‑GCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGa        <  1:412286/151‑1 (MQ=255)
                                                                                                                                             gaaaatCAATCCAATGTGGATCCAGTTGCACC‑GCCATCTGGATGACAGCCTGTAGTGCAAAGAGTGGTTGGGGGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGa        <  2:109921/148‑1 (MQ=255)
                                                                                                                                              gAAAACAATCCAATGTGGATCCAGTTGCACA‑GCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGTTGGGCGATCACAAACAGATGCTCGCTGCGTTAATCAAAAAATATGCCCGAGCGGCGACGCTGTCAATGTGGCAGCATCTGGa        <  2:515111/149‑1 (MQ=255)
                                                                                                                                              gAAAACAATCCAATGTGGATCCAGTTGCACA‑GCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGaaa      <  1:138695/151‑1 (MQ=255)
                                                                                                                                               aaaaCAATCCAATGTGGATCCAGTTGCACA‑GCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGaaaa     <  2:121372/151‑1 (MQ=255)
                                                                                                                                                  aCAATCCAATGTGGATCCAGTTGCACA‑GCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGt  >  2:277068/1‑151 (MQ=255)
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ATTGCAACTGGAAGAGCGTGAACTGGCTTCCAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATGTGGATCCAGTTGCACA‑GCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGT  >  NC_000913/4554959‑4555255

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑