Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 4,573,496 | 0 | A | G | 41.9% | 53.8 / 22.3 | 43 | pseudogene (1083/1503 nt) | yjiT | pseudogene |
Reads supporting (aligned to +/- strand): ref base A (1/24); new base G (18/0); total (19/24) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 3.12e-11 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TTGTGAAGATGGTGAGGAAGTCTCTGGCCTTGGGCCAGCCTATGCTTCTCTGGAAAACAGACAGGCAACAGTTCGATTACGTAAAAGCGAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGA‑TTGTTGGGAGCATTAAACTTGATGAC‑AG‑TG‑AAATTGCCA‑TTGGTGAGGTG‑CCGTTAACCTTCATCG‑TTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTGCAGTGTGCGAAGCAGCGATGTTCTGATTGTGCTCCC > NC_000913/4573349‑4573645 | ttGTGAAGATGGTGAGGAAGTCTCTGGCCTTGGGCCAGCCTATGCTTCTCTGGAAAACAGACAGGCAACAGTTCGATTACGTAAAAGCGAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGGGCTgggggg > 1:128952/1‑151 (MQ=255) tgAAGATGGTGAGGAAGTCTCTGGCCTTGGGCCAGCCTATGCTTCTCTGGAAAACAGACAGGCAACAGTTCGATTACGTAAAAGCGAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGGGCTggggggg > 1:183957/1‑148 (MQ=255) tgAAGATGGTGAGGAAGTCTCTGGCCTTGGGCCAGCCTATGCTTCTCTGGAAAACAGACAGGCAACAGTTCGATTACGTAAAAGCGAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGGGCTGGGGGggtg > 1:333838/1‑151 (MQ=255) aTGGTGAGGAAGTCTCTGGCCTTGGGCCAGCCTATGCTTCTCTGGAAAACAGACAGGCAACAGTTCGATTACGTAAAAGCGAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGGTGGGGGGGTGG‑ttgt > 1:535474/1‑151 (MQ=255) ggTGAGGAAGTCTCTGGCCTTGGGCCAGCCTATGCTTCTCTGGAAAACAGACAGGCAACAGTTCGATTACGTAAAAGCGAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGA‑ttgtt < 2:671645/150‑1 (MQ=255) ggTGAGGAAGTCTCTGGCCTTGGGCCAGCCTATGCTTCTCTGGAAAACAGACAGGCAACAGTTCGATTACGTAAAAGCGAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGA‑ttgtt < 1:2813/150‑1 (MQ=255) ggTGAGGAAGTCTCTGGCCTTGGGCCAGCCTATGCTTCTCTGGAAAACAGACAGGCAACAGTTCGATTACGTAAAAGCGAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGGTTGTCGTTAGTGGCTCGTGGTGGGGGGGGGg‑ttgtt > 2:732100/1‑150 (MQ=255) gAGGAAGTCTCTGGCCTTGGGCCAGCCTATGCTTCTCTGGAAAACAGACAGGCAACAGTTCGATTACGTAAAAGCGAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGA‑TTGTTggg < 1:430816/150‑1 (MQ=255) ggAAGTCTCTGGCCTTGGGCCAGCCTATGCTTCTCTGGAAAACAGACAGGCAACAGTTCGATTACGTAAAAGCGAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGA‑TTGTTGGGAGc < 1:331746/151‑1 (MQ=255) aaGTCTCTGGCCTTGGGCCAGCCTATGCTTCTCTGGAAAACAGACAGGCAACAGTTCGATTACGTAAAAGCGAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGA‑TTGTTGGGAGCAt < 2:110430/151‑1 (MQ=255) ccTTGGGCCAGCCTATGCTTCTCTGGAAAACAGACAGGCAACAGTTCGATTACGTAAAAGCGAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGA‑TTGTTGGGAGCATTAAACTtgat < 1:103069/151‑1 (MQ=255) ccAGCCTATGCTTCTCTGGAAAACAGACAGGCAACAGTTCGATTACGTAAAAGCGAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGA‑TTGTTGGGAGCATTAAACTTGATGAC‑AG‑Tg < 1:130624/151‑1 (MQ=255) aGCCTATGCTTCTCTGGAAAACAGACAGGCAACAGTTCGATTACGTAAAAGCGAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGGGCTGGGGGGGTGA‑TTGTTGGGGGGATTTAAATTTATGAC‑a > 1:172773/1‑146 (MQ=255) gCCTATGCTTCTCTGGAAAACAGACAGGCAACAGTTCGATTACGTAAAAGCGAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGA‑TTGTTGGGAGCATTAAACTTGATGAC‑AG‑TG‑aaa < 2:71036/151‑1 (MQ=255) aTGCTTCTCTGGAAAACAGACAGGCAACAGTTCGATTACGTAAAAGCGAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGA‑TTGTTGGGAGCATTAAACTTGATGAC‑AG‑TG‑AAATTg < 1:18840/150‑1 (MQ=255) tctcTGGAAAACAGACAGGCAACAGTTCGATTACGTAAAAGCGAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGA‑TTGTTGGGAGCATTAAACTTGATGAC‑AG‑TG‑AAATTGCCA‑tt < 1:187539/150‑1 (MQ=255) ctGGAAAACAGACAGGCAACAGTTCGATTACGTAAAAGCGAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGA‑TTGTTGGGAGCATTAAACTTGATGAC‑AG‑TG‑AAATTGCCA‑TTg < 1:100443/148‑1 (MQ=255) ctGGAAAACAGACAGGCAACAGTTCGATTACGTAAAAGCGAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGA‑TTGTTGGGAGCATTAAACTTGATGAC‑AG‑TG‑AAATTGCCA‑TTg < 2:451806/148‑1 (MQ=255) aaaaCAGACAGGCAACAGTTCGATTACGTAAAAGCGAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGA‑TTGTTGGGAGCATTAAACTTGATGAC‑AG‑TG‑AAATTGCCA‑TTGGTGAGGt < 1:638710/151‑1 (MQ=255) aaaCAGACAGGCAACAGTTCGATTACGTAAAAGCGAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGA‑TTGTTGGGAGCATTAAACTTGATGAC‑AG‑TG‑AAATTGCCA‑TTGGTGAGGTg < 1:436565/151‑1 (MQ=255) aaCAGACAGGCAACAGTTCGATTACGTAAAAGCGAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCCGGGTGGGGGGGTGG‑TTGTTGGGGGGATTTAACTTTATGAC‑AG‑GG‑AAATTTCCC‑tttggggggggg > 1:317814/1‑141 (MQ=255) ggCAACAGTTCGATTACGTAAAAGCGAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGGGCTGGGGGGGGGA‑TTTTTGGGGGCATTAAAATTGGTGAG‑AGAGG‑AAATTG‑CA‑TTTGGGGGGTG‑CCCTTAAcc > 1:3634/1‑151 (MQ=255) cAGTTCGATTACGTAAAAGCGAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGA‑TTGTTGGGAGCATTAAACTTGATGAC‑AG‑TG‑AAATTGCCA‑TTGGTGAGGTG‑CCGTTAACCTTCAt < 1:510979/151‑1 (MQ=255) ttCGATTACGTAAAAGCGAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGGTGGGGGGGTGG‑TTTTTTGGGGCATTTAAATTTATGAC‑AA‑TG‑GAAATGGCA‑TTGGTGGGGGG‑GCGTTTAcc > 2:70146/1‑143 (MQ=255) tCGATTACGTAAAAGCGAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGGGGTGGAGGGGTGA‑TTGTTTGGGGCATTTAACTTTATGAA‑AG‑GG‑GAAATGCCA‑TTGGGGGGGGG‑GCGTTTAACtt > 1:687645/1‑144 (MQ=255) cGATTACGTAAAAGCGAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGA‑TTGTTGGGAGCATTAAACTTGATGAC‑AG‑TG‑AAATTGCCA‑TTGGTGAGGTG‑CCGTTAACCTTCATCG‑TTg < 1:499542/151‑1 (MQ=255) gATTACGTAAAAGCGAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGGGGTGGGGGGGAGG‑TTTTTGGGGGGAATTAAATTGGTGACAAG‑GG‑AAATGGCAT‑TTGGGGGGGGG‑CGGTTAACC‑TCATCG‑tt > 1:579697/1‑149 (MQ=255) ttACGTAAAAGCGAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGGGGTGGGGGGGTGG‑TTTTTTGGGGCATTTAAATTGATGAC‑AG‑GG‑GAAATGGCA‑TTTGTGAGGGG‑GCGTTTACCCTCATCG‑TTTAt > 1:674672/1‑150 (MQ=255) aaaaGCGAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGGTGGGGGGGGGATTTTTTGGGGGCGTTAAA‑TTTATTAA‑AG‑TGAAAATTTCCT‑TTGGGGGGGGG‑CCGTTAA‑CTTCATCG‑t > 2:729073/1‑140 (MQ=255) gAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGGGGTGGGGGGGGGG‑TTGTTTGGGGGATTTAAATTTATGGC‑AG‑GG‑AAAATGTCA‑TTTGTGGGGGG‑GCGTTAAACCTTTTCG‑TTGATGGAGATCa > 1:279367/1‑146 (MQ=255) gAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGGGCTGGGGGGGTGG‑TTTTTGGGAGCATTAAAAATGATGAC‑AG‑GG‑GAATTGTCA‑TTTGTGAGGGGCCCGTTACCCTT‑ATCG‑TTTATTCAGATCaa > 1:423087/1‑147 (MQ=255) aGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGGGGTGGGGGGGTGA‑TTGTTTGGGGGATTTAAATTGATGAC‑AG‑GG‑GAATTTCCC‑TTGGGGGGGGG‑CCGTTTAACTTTATCC‑TTGATGGAGATAAATg > 1:230083/1‑147 (MQ=255) tGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGGGGGGTGA‑TTTTTGGGGGCATTTAAATTTGTGTC‑AG‑GG‑AAAATGCCATTTGGGGGGGGG‑CGGTTAA‑CTTTATCG‑GTGATGCAGATCAAtaggtg > 1:745520/1‑144 (MQ=255) tGAGATTTGGCAGGCAAAATCCATCGGCAGGGTTGTCGTTAGTGGCTCCTGGTGGGGGGGGGA‑TTTTTGGGGGGATTTAAATTTTTTGC‑AG‑GG‑TAAAATTCA‑CTGGGGAGGTG‑GCGTTTACCTTTTTCT‑TTGGTGTCGATCAATTGCTGt > 2:315122/1‑150 (MQ=255) gagaTTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGA‑TTGTTGGGAGCATTAAACTTGATGAC‑AG‑TG‑AAATTGCCA‑TTGGTGAGGTG‑CCGTTAACCTTCATCG‑TTGATGCAGATCAATGGCTGtt < 1:200224/150‑1 (MQ=255) gagaTTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGA‑TTGTTGGGAGCATTAAACTTGATGAC‑AG‑TG‑AAATTGCCA‑TTGGTGAGGTG‑CCGTTAACCTTCATCG‑TTGATGCAGATCAATGGCTGTTa < 2:325400/151‑1 (MQ=255) aTTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCTTGGTGGGGGGGGGG‑TTGTTTGGGGCATTAAAATTTATGAC‑AG‑GG‑AAAATTTCA‑TTGGTGGGGGG‑CCGGTAAACTTCCTTGTTTGAT‑CAGATCAAttggt > 2:716967/1‑140 (MQ=255) ttGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCCGGGTTGGGGGGTGA‑TTGTTTGGGGGACTTAAATTGTAGAG‑AG‑GG‑GAAATTCCA‑TTGGTGGGGGG‑GCGGTTAACTTCCTCG‑GTTGTGGAGATCAATTGCTGTTTTAggg > 1:488124/1‑151 (MQ=255) aggcaAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGGTGGGGGGGTGA‑TTTTTGGGAGGATTTAAATTGATGAC‑AG‑TG‑GAATTGCCA‑TTGGGGAGGGG‑GCGTTTACCTTCCTCG‑TTGATGGAGATCAATAGGTGTTACAggggaagg > 1:717138/1‑146 (MQ=255) aaTCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGA‑TTGTTGGGAGCATTAAACTTGATGAC‑AG‑TG‑AAATTGCCA‑TTGGTGAGGTG‑CCGTTAACCTTCATCG‑TTGATGCAGATCAATGGCTGTTACAGGGACAGGcttctt < 2:187046/151‑1 (MQ=255) gCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGA‑TTGTTGGGAGCATTAAACTTGATGAC‑AG‑TG‑AAATTGCCA‑TTGGTGAGGTG‑CCGTTAACCTTCATCG‑TTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTGCAGTGTGc < 2:183951/151‑1 (MQ=255) aGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGA‑TTGTTGGGAGCATTAAACTTGATGAC‑AG‑TG‑AAATTGCCA‑TTGGTGAGGTG‑CCGTTAACCTTCATCG‑TTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTGCAGTGTGCGa < 2:186487/151‑1 (MQ=255) aGTGGCTCGTGCTGGAGGGATGA‑TTGTTGGGAGCATTAAACTTGATGAC‑AG‑TG‑AAATTGCCA‑TTGGTGAGGTG‑CCGTTAACCTTCATCG‑TTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTGCAGTGTGCGAAGCAGCGATGtt < 1:604359/151‑1 (MQ=255) gCTGGAGGGATGA‑TTGTTGGGAGCATTAAACTTGATGAC‑AG‑TG‑AAATTGCCA‑TTGGTGAGGTG‑CCGTTAACCTTCATCG‑TTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTGCAGTGTGCGAAGCAGCGATGTTCTGATtgtg < 2:128944/150‑1 (MQ=255) gAGGGATGA‑TTGTTGGGAGCATTAAACTTGATGAC‑AG‑TG‑AAATTGCCA‑TTGGTGAGGTG‑CCGTTAACCTTCATCG‑TTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTGCAGTGTGCGAAGCAGCGATGTTCTGATTGTGCTccc < 1:235540/151‑1 (MQ=255) | TTGTGAAGATGGTGAGGAAGTCTCTGGCCTTGGGCCAGCCTATGCTTCTCTGGAAAACAGACAGGCAACAGTTCGATTACGTAAAAGCGAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGA‑TTGTTGGGAGCATTAAACTTGATGAC‑AG‑TG‑AAATTGCCA‑TTGGTGAGGTG‑CCGTTAACCTTCATCG‑TTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTGCAGTGTGCGAAGCAGCGATGTTCTGATTGTGCTCCC > NC_000913/4573349‑4573645 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |