Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	354,288	0	T	G	41.8%	96.7 / 12.5	55	V528G (GTG→GGG)	prpE	propionate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base T (4/28);  new base G (23/0);  total (27/28)
Fisher's exact test for biased strand distribution p-value = 9.40e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGG  >  NC_000913/354141‑354434                                                                                                                                                    |                                                                                                                                                    ggggCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGaa                                                                                                                                                  <  1:401699/151‑1 (MQ=255)  gggCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGaaa                                                                                                                                                 <  2:637944/151‑1 (MQ=255)            gACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTG‑a                                                                                                                                      <  2:32669/151‑1 (MQ=255)            gACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGATggg                                                                                                                                           >  2:402065/1‑145 (MQ=255)             aCGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATgcgc                                                                                                                                          >  1:245111/1‑147 (MQ=255)              cGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTG‑aaa                                                                                                                                    <  2:438590/151‑1 (MQ=255)              cGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGGAAGaatcggt                                                                                                                                         >  2:168666/1‑141 (MQ=255)              cGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATgc                                                                                                                                            >  1:213489/1‑144 (MQ=255)                 tGACGGTTATCACTTTATTCTCGGGCGCACTGACGGTGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTTGGTGGGGGGAAAAATGGGCCG‑GAAggg                                                                                                                                 >  2:65317/1‑151 (MQ=255)                    cGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTG‑AAAGGGCa                                                                                                                               <  1:78943/150‑1 (MQ=255)                     ggTGATCACGTTATTCTCGGGCGCGCTGACGATGTAATGATCGTAGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTTCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATACGCTG‑ATAGGGcc                                                                                                                               <  2:273753/149‑2 (MQ=255)                      gTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTG‑AAAGGGCAgg                                                                                                                             >  1:395636/1‑150 (MQ=255)                              tttATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTG‑AAAGGGCAggggggggggg                                                                                                                    >  1:244037/1‑151 (MQ=255)                                tATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGGAAGGTGCGCCG‑GAAGGGCAggggggggggggg                                                                                                                  >  2:326507/1‑149 (MQ=255)                                      cGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGttt                                                                                                               <  1:394436/148‑1 (MQ=255)                                      cGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGttt                                                                                                               <  2:428027/148‑1 (MQ=255)                                         gcgcACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCAtt                                                                                                         <  1:65523/151‑1 (MQ=255)                                            cACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGGGCTG‑GAAGGGGAGGGGGGGGGGGGGGTTGTGAtt                                                                                                         >  2:200664/1‑148 (MQ=255)                                                 aCGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTG‑AAAAGGCAGGGGGCGGGGGGGTTTGTCATTCCGaa                                                                                                    >  1:246386/1‑148 (MQ=255)                                                  cGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGGAAGATGCGCTG‑GAAGGGCCGGGGGCGGGGGCGTTTGTCATTCCGaaa                                                                                                   >  1:667738/1‑148 (MQ=255)                                                      gTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGc                                                                                             <  1:198731/150‑1 (MQ=255)                                                                 tGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGa                                                                                  <  2:239673/150‑1 (MQ=255)                                                                 tGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGGGGCGGCGGGTGGGGGGAAAAAGGAGCTG‑AAACGGGAGGTGGGGGGGGCGGTTGTCCTTCCGAAAGAGAGCGACCGTcgg                                                                                    >  2:140850/1‑146 (MQ=255)                                                                  gCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGaa                                                                                 <  1:391864/150‑1 (MQ=255)                                                                   ccGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGa                                                                               <  2:571338/151‑1 (MQ=255)                                                                    cGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGAt                                                                              <  1:506761/151‑1 (MQ=255)                                                                    cGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGATGCGCCG‑AAAGGGGAGGTGGGGGGGGGGGTTTTTATTTCGCAAAAGAGCGagaggg                                                                                      >  2:729869/1‑141 (MQ=255)                                                                       gCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGt                                                                           <  1:399560/151‑1 (MQ=255)                                                                       gCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGCAAATGGGCTGAAAAGGGAGGGGGGGGGGGGGGTTTGT‑ATTTCGGAAAAGAGCGGCAGTCTGGaa                                                                                 >  2:620901/1‑145 (MQ=255)                                                                        cATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTG‑AAAGGGGAGGTGGGGGGGGGGGTTGTCATTCCGAAAGAGGGCGACAGTCTGGGAGATCg                                                                            >  1:539369/1‑149 (MQ=255)                                                                        cATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCCG‑AAAGGGGAGGTGGCGGTGGCGGTTGTCCTTCCGAAAAAGGGCGGCAGGCTGGGAGAGCGt                                                                           >  1:724888/1‑150 (MQ=255)                                                                         aTCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGa                                                                         <  2:407398/151‑1 (MQ=255)                                                                          tCGGCT‑GGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGTTGGGGGGAAAGATGCGCTT‑AAAAGGCAGGTGGCGGTGGGGTTTGTCATTCCCAAAAGAAGCGACAGTATGGGAGATCg                                                                            >  1:130121/1‑146 (MQ=255)                                                                            ggCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGAtgt                                                                      <  2:345514/151‑1 (MQ=255)                                                                               tGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGTGGAAGATGCGCTG‑AAAGGGCAGGGGGGGGGGGCGTTTGTCATTTCGAAAGAGGGGGACAGTCTGGGAGATCGTGATGTgg                                                                    >  1:37137/1‑150 (MQ=255)                                                                                     cgcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTc                                                             <  2:671434/151‑1 (MQ=255)                                                                                      gcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCg                                                            <  1:386127/151‑1 (MQ=255)                                                                                        gcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCa                                                          <  2:130113/151‑1 (MQ=255)                                                                                          gagaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTG‑GAAGGGCAGGGGGGGGGGGCGGTTGTCATTTCGAAAGAGAGCGACAGTCTGGAAGATCGTGGTGTGGCGCACTCGc                                                           >  2:736426/1‑148 (MQ=255)                                                                                               tGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTG‑AAGGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGaa                                                    <  1:665136/150‑1 (MQ=255)                                                                                               tGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTG‑AAAAGGCAGGTGGCGGGGGCGTTTGTCATTCCGAAAAAGAGCGACAGTCTGGAAGATCGGGATGTGGCGCACTCGc                                                           >  1:104628/1‑143 (MQ=255)                                                                                                 aaGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGc                                                 <  1:331318/151‑1 (MQ=255)                                                                                                 aaGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGc                                                 <  1:737098/151‑1 (MQ=255)                                                                                                  agagagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGAAGCGCTG‑AAAGGGCAGGTGGGGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATTGTGGTGTGGCGCAATCGCAAGGGAAgg                                                  >  1:563057/1‑149 (MQ=255)                                                                                                   gagagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGCTG‑AAAAGGCAGGGGGCGGGGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGGGATGTGGGGCACTCGCAAGAGAAGGCg                                                >  1:528128/1‑150 (MQ=255)                                                                                                              cAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCtg                                    <  1:260972/151‑1 (MQ=255)                                                                                                              cAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGCTG‑AAAGGGCAGGGGGCGGGGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGGTGTGGCGCACTCGCAAGCGAAGGCGATTATGGCGCtg                                    >  1:424651/1‑151 (MQ=255)                                                                                                               aGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAAATGCGCCG‑AAAGGGCCGGGGGCGGGGGCGTTTTTTATTCCGAAAGAGAGCGACAGTCTGGAAGATCGGGATGTGGGGCACTCGCAAGAGAAGGCGATTATGGCGCtgg                                   >  2:672027/1‑151 (MQ=255)                                                                                                                gCCATCCGGGCGTTGCCGGAGTGGCGGTGGTTGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCtggt                                  <  2:72609/151‑1 (MQ=255)                                                                                                                    tCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATGCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGa                               <  2:510802/150‑1 (MQ=255)                                                                                                                         gCGTTGCCGAAGTGGCGGTGGTGGGGGGGGAAAATGCGCTG‑AAAGGGGAGGTGGCGGGGGGGTTTGTCATTCCGAAAGAGAGCGACAGGCTGGAAGATCGTGATGTGGCGCACTCGCCAGAGAAAGCGAGTATTGCGCTGGGGGACAGcc                          >  2:680221/1‑150 (MQ=255)                                                                                                                                       gCGGTGGTTGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTg           >  1:636125/1‑151 (MQ=255)                                                                                                                                          gtggtTGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGgcc        >  2:180506/1‑151 (MQ=255)                                                                                                                                                ggggTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCgg  <  1:631971/151‑1 (MQ=255)                                                                                                                                                ggggTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCg   <  1:693108/150‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                    GGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGG  >  NC_000913/354141‑354434

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑