Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	718,659	0	T	A	56.7%	14.6 / 40.5	30	D601V (GAT→GTT)	speF	ornithine decarboxylase isozyme, inducible
Reads supporting (aligned to +/- strand):  ref base T (3/10);  new base A (17/0);  total (20/10)
Fisher's exact test for biased strand distribution p-value = 9.52e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.53e-06

TCTGCATCCGGCAGACGAACCAGTTCCACTTCACCGCGTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGACTCAAGCAGTTTTTCGAAGCGTACCAGCAGGGCAACAAGTTGCTGA  >  NC_000913/718515‑718805                                                                                                                                                 |                                                                                                                                                   tCTGCATCCGGCAGACGAACCAGTTCCACTTCACCGCGTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGGATACAAAACa                                                                                                                                                  >  2:36744/1‑147 (MQ=255)   tGCATCCGGCAGACGAACCAGTTCCACTTCACCGCGTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGGATACAAAACATACAtt                                                                                                                                            >  2:350030/1‑151 (MQ=255)    gCATCCGGCAGACGAACCAGTTCCACTTCACCGCGTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATTCAttt                                                                                                                                           <  2:70047/151‑1 (MQ=255)      aTCCGGCAGACGAACCAGTTCCACTTCACCGCGTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGGATACAAAACAATCAttt                                                                                                                                           >  1:314588/1‑149 (MQ=255)            cAGACGAACCAGTTCCACTTCACCGCGTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCCTACAAAACAAGCATTTTCTCacac                                                                                                                                   >  1:199784/1‑151 (MQ=255)                 gAACCAGTTCCACTTCACCGCGTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTg                                                                                                                              <  2:718027/151‑1 (MQ=255)                  aaCCAGTTCCACTTCACCGCGTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAAACATGCCTTTTCTTTCACaa                                                                                                                                 >  2:297017/1‑147 (MQ=255)                    ccAGTTCCACTTCACCGCGTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCCTAAAAAATATTTATTTTCTTACACaa                                                                                                                                 >  1:71367/1‑145 (MQ=255)                     cAGTTCCACTTCACCGCGTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAAAAATGCATTTTCTTACACAACTGACg                                                                                                                           >  1:278524/1‑150 (MQ=255)                                 aCCGCGTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGGATGCAAAACATTCATTTTTTGCCACACAAGACGGAgggggtaa                                                                                                                 >  1:143294/1‑143 (MQ=255)                                        aaaTAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAg                                                                                                        <  1:43159/150‑1 (MQ=255)                                                gTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAAACATGCATTTTCTTGCAAAACCGACGCAGGGGAAAAACGGCGGAGCGCGCtt                                                                                                 >  1:52426/1‑149 (MQ=255)                                                  aGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCAt                                                                                              <  1:479731/150‑1 (MQ=255)                                                        cttcttGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAAACAAGCATTTTCTGACACAAATGACGCAGGGGATAAACCGCGGAGCGCTCTTCATGCTGttt                                                                                       >  1:555101/1‑151 (MQ=255)                                                           cttGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTg                                                                                      <  2:406579/149‑1 (MQ=255)                                                            ttGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGGGTAAAAAAAATGTATTTTCTGACACAAAAGAAGCCGGGGAGAAACCGCGGCGCGCGCTTCTTGCTGtttt                                                                                      >  2:228632/1‑147 (MQ=255)                                                               cGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATg                                                                                <  1:26530/151‑1 (MQ=255)                                                               cGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAAAAATTCATTTTCTTACCCAACTGACGCCGGGGATAAACGGCGGAGGGctccttcttcttgtttt                                                                                     >  2:710039/1‑132 (MQ=255)                                                                 gATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGa                                                                              <  1:743957/151‑1 (MQ=255)                                                                     cATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCCTACAAAACATGCATTTTCTTACACAACTTACGCCGGGGGTAAACCGCGTAAAGCTCTTCATGCTGTTTTTTGTTGGAAg                                                                            >  1:696303/1‑149 (MQ=255)                                                                              gcgTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCt                                                                 <  2:120701/151‑1 (MQ=255)                                                                                gTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCCGACAAAACAATCCTTTTCTGTCACCACTGACGCCGGGGATAAACCGCGGAGGGCCCCTTTTTCTGTTTTTAGATGggagggagacctttt                                                                 >  2:522662/1‑142 (MQ=255)                                                                                  gggAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCt                                                              <  1:50830/150‑1 (MQ=255)                                                                                  gggAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAAAAATGCATTTTCTGACACAACAGACGCAGGGGATAACCGGCGGAGCGCTCTTCATGCTGTTTTTTGATGGAAGGGAGCACTtcttct                                                              >  2:105033/1‑150 (MQ=255)                                                                                          cTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAAACATGCATTTTCTTACACCAATGACGCAGGGGATAAACCGCGGAGCGCTCTTCCTTCTGTTTTTAGATGGGAGGGAGCACTTTTGCTTATGGcgcg                                                     >  1:402886/1‑151 (MQ=255)                                                                                                 cGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGACt                                              <  2:246191/151‑1 (MQ=255)                                                                                                           ctTTTTGCAGTTGTTTCACGTTGTGGCGGGCATAAAAAAAATGCTTTTTCTGGCAAAACATACGCAGGGGATAAACGGCGAAGCGCTCTTCATGCTGTTTGTTGAGGGGAGGGGGCAAATCTTCTAATAGCGCGTCGGGCTCAAGCAGttt                                    >  2:138540/1‑151 (MQ=255)                                                                                                                       gttTCACGTTGTGGCGGGGATAAAAATAATGCCTTTCTTTGCACAAATCAAGCAGGGGGTAAACGGCGGAGCGCTCCTCATGTTGTTTGTAGATGGAAGGTAGCACTTCTTCTAATGGGGCGTGGGGGCCAAGCCGgttttttatgcgt                          >  2:311807/1‑141 (MQ=255)                                                                                                                                       ggCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGACTCAAGCAGTTTTTCGAAGCGTACCAGCAGGGCAACAAGt        >  1:427677/1‑151 (MQ=255)                                                                                                                                               aaTCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGACTCAAGCAGTTTTTCGAAGCGTACCAGCAGGGCAACAAGTTGCTGa  >  1:257311/1‑149 (MQ=255)                                                                                                                                                 |                                                                                                                                                   TCTGCATCCGGCAGACGAACCAGTTCCACTTCACCGCGTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGACTCAAGCAGTTTTTCGAAGCGTACCAGCAGGGCAACAAGTTGCTGA  >  NC_000913/718515‑718805

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑