Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,574,0510GT43.2% 55.9 / 14.5 37V384V (GTC→GTA) pqqLputative periplasmic M16 family zinc metalloendopeptidase
Reads supporting (aligned to +/- strand):  ref base G (2/19);  new base T (16/0);  total (18/19)
Fisher's exact test for biased strand distribution p-value = 1.19e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGACGTTTCGAAAGTTGATATGTCTCTTCCGGCGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACGGAAAAACAGCGACTGATAATCGGGTGCAATTTTAAC  >  NC_000913/1573901‑1574194
                                                                                                                                                      |                                                                                                                                               
caGACGTTTCGAAAGTTGATATGTCTCTTCCGGCGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTg                                                                                                                                                 <  1:531157/151‑1 (MQ=255)
    cGTTTCGAAAGTTGATATGTCTCTTCCGGCGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGAcat                                                                                                                                             <  1:467416/151‑1 (MQ=255)
             aGTTGATATGTCTCTTCCGGCGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGtt                                                                                                                                    <  1:543055/151‑1 (MQ=255)
             aGTTGATATGTCTCTTCCGGCGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGtt                                                                                                                                    <  1:422083/151‑1 (MQ=255)
                         tctTCCGGCGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGGGGATTTTTCCTCCTCCGGTTCTTCCGCCGaa                                                                                                                         >  2:701068/1‑150 (MQ=255)
                            tCCGGCGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTTCAATCATCGAGTTTTTCCGCCGaaaaa                                                                                                                      >  1:538918/1‑150 (MQ=255)
                               ggCGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTTACATACTCGGGTTTTTTTACAGaaaaa                                                                                                                      >  2:53335/1‑147 (MQ=255)
                                 cGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTTAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTTTCATCATCGAGGTCTTCAGCAGAAAAAcc                                                                                                                    >  1:84673/1‑147 (MQ=255)
                                       gtaCGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGGAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGAt                                                                                                            <  2:322994/147‑1 (MQ=255)
                                        aaCGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCaa                                                                                                         <  1:610365/151‑1 (MQ=255)
                                          cGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATg                                                                                                       <  1:162214/151‑1 (MQ=255)
                                            gCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTTAAATCATCGAGTTTTTCAGCAGAAAAACCAAGCTGATCAATgg                                                                                                      >  1:538268/1‑150 (MQ=255)
                                             cGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTTAAATCATCGAGGTCTTCTGCAGAAAAACCACGCTGATCaa                                                                                                         >  2:288410/1‑146 (MQ=255)
                                                 ttattTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTTACATCATCGAGTTCTTTCGCAGAAAAAACATGCCGGTCAATGGTTGcc                                                                                                 >  2:162956/1‑150 (MQ=255)
                                                   attTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAAc                                                                                              <  1:657796/151‑1 (MQ=255)
                                                              tGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGGAGATTTTACATAATCGAGGTCTTTAACCAAAAAAAAAAGCAGATTAATGGTTGCCAAcccctccctt                                                                                     >  1:63921/1‑140 (MQ=255)
                                                                  tGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATTATCGAGGTCTTTAGCAGAAAAAACAAGCTGATCAATGGTTGCCAAATCTGcc                                                                                        >  1:589264/1‑142 (MQ=255)
                                                                   gCCATGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGACATTAATGCa                                                                               <  2:610566/150‑1 (MQ=255)
                                                                   gCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCa                                                                               <  2:178398/150‑1 (MQ=255)
                                                                    ccAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTTGTATCAACCCGTTTTTTCACAGAAAAAACAAGCCGATTAAAGGTTGCCAACCCTTCCCTTAATGCAtt                                                                             >  2:425305/1‑151 (MQ=255)
                                                                       ggCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTTAAATCATCGGGGTTTTTAACCAAAAAAACAAGCTGATTAATGGGTGGCCAATCCCCCATTAATTCaattgc                                                                          >  1:81000/1‑146 (MQ=255)
                                                                         cGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTTTGATCCTCCAGGTCTTTATCAGAAAAAACATGCTTGTCAAAGGTTGTCAACCCTGCCCTTTATGCATTTgc                                                                          >  2:325516/1‑149 (MQ=255)
                                                                              ggTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCAt                                                                     <  2:420198/149‑1 (MQ=255)
                                                                              ggTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGGAGGTTTTACAACATCGAGGTCTTTATCAGAAAAAACAAGCTGATCAATGGGTGCCAAATCTTCCCTTAAATCATTTGCAGCATcc                                                                   >  1:259493/1‑151 (MQ=255)
                                                                                 cAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTg                                                                 <  2:137500/150‑1 (MQ=255)
                                                                                    cATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCat                                                              <  2:560574/150‑1 (MQ=255)
                                                                                     aTACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCatat                                                            <  2:101382/151‑1 (MQ=255)
                                                                                            aaTCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTTTCATCATCGAGGTTTTTAGCAGAAAAAACAATCTGATCAATGGTTGCCCACACTTCCCTTTATTCATTTTCAGCATCCCGCATTTTTTCGt                                                      >  2:304066/1‑150 (MQ=255)
                                                                                             aTCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCATATTATCGtct                                                    <  2:657718/151‑1 (MQ=255)
                                                                                                     cAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCAtt                                            <  2:712043/151‑1 (MQ=255)
                                                                                                           gCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGCTAAATTTTTGATCATCGAGTTTTTCTGCAGAAAAAACATGCTGATTACAAGTTGTCAACACTTCCCTTTATTCATTTTCAGCATCCTTCATATTATTGTCTCCTGCAttt                                           >  2:496673/1‑146 (MQ=255)
                                                                                                            cTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGGAGATTTTAAATTAACCGCATCTTCCGCCGAAAAACCACGCTGATCAAAGGGTGCCCAACCTGCCCTTTATGCACTTGCAGCATCCCGCGTATTTTTGTCTCGTGCACTTTTACgg                                     >  2:75543/1‑151 (MQ=255)
                                                                                                              gATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTTACATCATCGAGTTCTTCAGCAGAAAAAACAAGCTGGTCAAAGGGTGCCCACTCTTCCCTTAATGCATTTTCAGCCTCCTTCATATTATCGTCTCGTGCATTTTCACgg                                     >  1:328473/1‑149 (MQ=255)
                                                                                                              gATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACGGaa                                   <  2:269088/151‑1 (MQ=255)
                                                                                                              gATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACGGa                                    <  1:215353/150‑1 (MQ=255)
                                                                                                                              cAGCCAGGTGAGGCGGGTTGATTTGACATCATCGAGTTCTTCAGCAGGAACACCATGCTGATCAATGGTTGCCATCTCTGCCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACGGAAAAACAGCGACTGATaa                   <  2:467250/151‑1 (MQ=255)
                                                                                                                                        aGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACGGAAAAACAGCGACTGATAATCGGGTGCaa         <  1:617068/151‑1 (MQ=255)
                                                                                                                                               tAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACGGAAAAACAGCGACTGATAATCGGGTGCAATTTTAAc  >  2:448745/1‑151 (MQ=255)
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CAGACGTTTCGAAAGTTGATATGTCTCTTCCGGCGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACGGAAAAACAGCGACTGATAATCGGGTGCAATTTTAAC  >  NC_000913/1573901‑1574194

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑