Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,347,775	C→A	E380* (GAG→TAG)	rnb ←	ribonuclease II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,347,775	0	C	A	100.0%	212.2 / NA	54	E380* (GAG→TAG)	rnb	ribonuclease II
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (24/30);  total (24/30)

TGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTCGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGTTGGCAAATTTGCGCTAGCAAACGGACTTGTTCGGCAATTGCT  >  NC_000913/1347629‑1347920                                                                                                                                                   |                                                                                                                                                  tGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTAGGcg                                                                                                                                               >  2:665903/1‑151 (MQ=255)  gCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTAGGcg                                                                                                                                               <  1:152843/150‑1 (MQ=255)   ccAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTAGGcgac                                                                                                                                             <  2:605607/151‑1 (MQ=255)      gcgcGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTAGGCGACGat                                                                                                                                          >  2:132457/1‑151 (MQ=255)         cgTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTAGGCGACGATATc                                                                                                                                       <  1:421139/151‑1 (MQ=255)           tCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTAGGCGACGATATcc                                                                                                                                      >  2:148553/1‑150 (MQ=255)            cGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTAGGCGACGATATCCa                                                                                                                                     <  2:209953/150‑1 (MQ=255)            cGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTAGGCGACGATATCCAg                                                                                                                                    <  2:83024/151‑1 (MQ=255)                aTTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGTGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTAGGCGACGATATCCAGCACt                                                                                                                                <  1:88438/151‑1 (MQ=255)                        gATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTAGGCGACGATATCCAGCACTTCACCttt                                                                                                                        >  1:16447/1‑151 (MQ=255)                          tCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTAGGCGACGATATCCAGCACTTCACCttttt                                                                                                                      >  1:217511/1‑151 (MQ=255)                           cAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTAGGCGACGATATCCAGCACTTCACCttttt                                                                                                                      <  2:95704/150‑1 (MQ=255)                           cAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTAGGCGACGATATCCAGCACTTCACCTTTTTc                                                                                                                     >  2:565259/1‑151 (MQ=255)                           cAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTAGGCGACGATATCCAGCACTTCACCTTTTTc                                                                                                                     >  2:489989/1‑151 (MQ=255)                                 ccATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTAGGCGACGATATCCAGCACTTCACCTTTTTCACCgag                                                                                                               <  1:85731/151‑1 (MQ=255)                                  cATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTAGGCGACGATATCCAGCACTTCACCTTTTTCACCgaga                                                                                                              <  1:34747/151‑1 (MQ=255)                                    tatGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTAGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGaa                                                                                                             >  2:142834/1‑150 (MQ=255)                                    tatGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTAGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAAt                                                                                                            >  2:307960/1‑151 (MQ=255)                                       gCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTAGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATaaa                                                                                                         <  2:274265/151‑1 (MQ=255)                                       gCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTAGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATaaa                                                                                                         <  1:32977/151‑1 (MQ=255)                                       gCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTAGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATaa                                                                                                          >  2:731269/1‑150 (MQ=255)                                           gTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTAGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCgg                                                                                                     >  1:372789/1‑151 (MQ=255)                                            ttATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTAGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGt                                                                                                    >  2:731573/1‑151 (MQ=255)                                                aGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTAGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAAt                                                                                                 <  1:44876/150‑1 (MQ=255)                                                aGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTAGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAAt                                                                                                 <  1:661813/150‑1 (MQ=255)                                                aGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTAGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAAt                                                                                                 <  2:311477/150‑1 (MQ=255)                                                aGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTAGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAATc                                                                                                >  1:205985/1‑151 (MQ=255)                                                    gCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTAGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAATCCggg                                                                                            <  2:595848/151‑1 (MQ=255)                                                    gCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTAGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAATCCgg                                                                                             <  1:244678/150‑1 (MQ=255)                                                            cGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTAGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAATCCGGGCGATCttt                                                                                    <  2:273259/151‑1 (MQ=255)                                                                         cAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTAGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCg                                                                       >  1:108012/1‑151 (MQ=255)                                                                                      gCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTAGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGcc                                                          >  1:13209/1‑151 (MQ=255)                                                                                       cacaAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTAGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGcc                                                          >  2:254389/1‑150 (MQ=255)                                                                                        acaAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTAGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCAc                                                        <  1:303959/151‑1 (MQ=255)                                                                                          aaaTGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTAGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTc                                                      <  2:129176/151‑1 (MQ=255)                                                                                           aaTGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTAGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTc                                                      >  2:144507/1‑150 (MQ=255)                                                                                            aTGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTAGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTcgc                                                    <  2:371304/151‑1 (MQ=255)                                                                                              gTTAGCGGCAATCATCGCTTCCTCGTCGATACGGTTGGCAATGCGACGAGGCTAGGCGACGATATCCAGCCCTTCTCCTTTTTCCCCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGATTATGACGCATCTcgcc                                                   <  2:76666/150‑1 (MQ=255)                                                                                                     gCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTAGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGtt                                           <  2:523927/151‑1 (MQ=255)                                                                                                      cAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTAGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGTTg                                          <  1:389456/151‑1 (MQ=255)                                                                                                       aaTCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTAGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGTTgg                                         <  1:231078/151‑1 (MQ=255)                                                                                                           atcGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTAGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGTTGGCaa                                      >  2:214625/1‑150 (MQ=255)                                                                                                             cGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTAGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGTTGGCAAAtt                                   >  2:591965/1‑151 (MQ=255)                                                                                                             cGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTAGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGTTGGCAAAt                                    <  1:132464/150‑1 (MQ=255)                                                                                                                   ttcGACGATACGGTTGGCAATGCGACGAGGCTAGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGTTGGCAAATTTGCGCt                             >  2:102589/1‑151 (MQ=255)                                                                                                                    tcGACGATACGGTTGGCAATGCGACGAGGCTAGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGTTGGCAAATTTGCGCt                             >  2:659753/1‑150 (MQ=255)                                                                                                                                 tGGCAATGCGACGAGGCTAGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGTTGGCAAATTTGCGCTAGCAAACGGACtt                <  1:356753/150‑1 (MQ=255)                                                                                                                                   gCAATGCGACGAGGCTAGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGTTGGCAAATTTGCGCTAGCAAACGGACTTGtt             >  1:338639/1‑151 (MQ=255)                                                                                                                                    cAATGCGACGAGGCTAGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGTTGGCAAATTTGCGCTAGCAAACGGACTTGTTc            >  1:203834/1‑151 (MQ=255)                                                                                                                                            cgaGGCTAGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGTTGGCAAATTTGCGCTAGCAAACGGACTTGTTCGGCAATTg    <  1:427274/151‑1 (MQ=255)                                                                                                                                            cgaGGCTAGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGTTGGCAAATTTGCGCTAGCAAACGGACTTGTTCGGCAATTg    <  2:157935/151‑1 (MQ=255)                                                                                                                                            cgaGGCTAGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGTTGGCAAATTTGCGCTAGCAAACGGACTTGTTCCGCAATTg    <  2:502181/151‑1 (MQ=255)                                                                                                                                             gaGGCTAGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGTTGGCAAATTTGCGCTAGCAAACGGACTTGTTCGGCAATTGc   <  2:593670/151‑1 (MQ=255)                                                                                                                                              aGGCTAGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATCAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGTTGGCAAATTTGCGCTAGCAAACGGACTTGTTCGGCAATTGCt  >  2:647179/1‑151 (MQ=255)                                                                                                                                                   |                                                                                                                                                  TGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTCGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGTTGGCAAATTTGCGCTAGCAAACGGACTTGTTCGGCAATTGCT  >  NC_000913/1347629‑1347920

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑