Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,931,977 T→G V366G (GTG→GGG)  purT → phosphoribosylglycinamide formyltransferase 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,931,9770TG97.4% 134.2 / ‑2.8 38coding (1097/1179 nt)purTphosphoribosylglycinamide formyltransferase 2
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base G (26/11);  minor base . (1/0);  total (27/11)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

TGGTCCTGCAGCTTCTGCCGTTATTCTGCCACAACTGACCAGTCAGAATGTCACGTTTGATAATGTGCAGAATGCCGTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCTGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTAC  >  NC_000913/1931834‑1932118
                                                                                                                                               |                                                                                                                                             
tGGTCCTGCAGCTTCTGCCGTTATTCTGCCACAACTGACCAGTCAGAATGTCACGTTTGATAATGTGCAGAATGCCGTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCTGGGGGGGGCACTg                                                                                                                                        >  1:531621/1‑151 (MQ=255)
       gCAGCTTCTGCCGTTATTCTGCCACAACTGACCAGTCAGAATGTCACGTTTGATAATGTGCAGAATGCCGTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCTGGGGGGGGCACTGGCTACt                                                                                                                                  <  1:571540/150‑1 (MQ=255)
          gCTTCTGCCGTTATTCTGCCACAACTGACCAGTCAGAATGTCACGTTTGATAATGTGCAGCATGCCGTAGGCGCAGTCTTGCAGATTCGTTTATTTGGTACGCCGGAAATTGCTGGCAGCCGCCGTATGGGGGGGGCACTgg                                                                                                                                       >  2:719386/1‑142 (MQ=255)
             tCTGCCGTTATTCTGCCACAACTGACCAGTCAGAATGTCACGTTTGATAATGTGCAGAATGCCGTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCTGGGGGGGGCACTGGCTACTGCag                                                                                                                              >  1:155865/1‑148 (MQ=255)
               tGCCGTTATTCTGCCACAACTGACCAGTCAGAATGTCACGTTTGATAATGTGCAGAATGCCGTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCTGGGGGGGGCACTGGCTACTGCagagag                                                                                                                          >  2:9445/1‑150 (MQ=255)
                   gTTATTCTGCCACAACTGACCAGTCAGAATGTCACGTTTGATAATGTGCAGAATGCCGTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCTGGGGGGGGCACTGGCTACTGCAGAGAGtgttg                                                                                                                     >  1:4829/1‑151 (MQ=255)
                   gTTATTCTGCCACAACTGACCAGTCAGAATGTCACGTTTGATAATGTGCAGAATGCCGTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCTGGGGGGGGCACTGGCTACTGCAGAGAGtgttg                                                                                                                     >  1:417991/1‑151 (MQ=255)
                     tATTCTGCCACAACTGACCAGTCAGAATGTCACGTTTGATAATGTGCAGAATGCCGTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCTGGGGGGGGCACTGGCTACTGCAGAGAGTgttgtt                                                                                                                   >  1:631398/1‑151 (MQ=255)
                            ccACAACTGACCAGTCAGAATGTCACGTTTGATAATGTGCAGAATGCCGTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCTGGGGGGGGCACTGGCTACTGCAGAGAGTGTTGTTGACGcc                                                                                                             >  1:394387/1‑150 (MQ=255)
                              acaACTGACCAGTCAGAATGTCACGTTTGATAATGTGCAGAATGCCGTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCTGGGGGGGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCAtt                                                                                                          >  2:682183/1‑151 (MQ=255)
                              acaACTGACCAGTCAGAATGTCACGTTTGATAATGTGCAGAATGCCGTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCTGGGGGGGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCAtt                                                                                                          >  2:340631/1‑151 (MQ=255)
                                 aCTGACCAGTCAGAATGTCACGTTTGATAATGTGCAGAATGCCGTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCTGGGGGGGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGa                                                                                                        >  1:57109/1‑150 (MQ=255)
                                       cAGTCAGAATGTCACGTTTGATAATGTGCAGAATGCCGTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCTGGGGGGGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAAcgcgcg                                                                                                 <  1:640883/151‑1 (MQ=255)
                                                 gTCACGTTTGATAATGTGCAGAATGCCGTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCTGGGGGGGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCAcgcc                                                                                        >  1:547578/1‑150 (MQ=255)
                                                             aaTGTGCAGAATGCCGTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCTGGGGGGGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTaa                                                                           <  1:48151/151‑1 (MQ=255)
                                                             aaTGTGCAGAATGCCGTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCTGGGGGGGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTa                                                                            >  2:435297/1‑150 (MQ=255)
                                                               tgtgCAGAATGCCGTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCTGGGGGGGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTaaa                                                                          >  1:619120/1‑150 (MQ=255)
                                                                    aGAATGCCGTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCTGGGGGGGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTAc                                                                     >  1:139930/1‑150 (MQ=255)
                                                                         gCCGTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCTGGGGGGGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGtt                                                               >  2:703155/1‑151 (MQ=255)
                                                                            gTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCTGGGGGGGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTaa                                                             >  1:638772/1‑150 (MQ=255)
                                                                                    aGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCTGGGGG‑GGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCaa                                                    >  1:77268/1‑150 (MQ=255)
                                                                                     gATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCTGGGGGGGGCACTGGCTACTGCAGAGAGAGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCaa                                                    >  1:663276/1‑150 (MQ=255)
                                                                                       tttGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCTGGGGGGGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCaaaa                                                  >  2:464759/1‑150 (MQ=255)
                                                                                        ttGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCTGGGGGGGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCaaaaa                                                 <  2:417975/150‑1 (MQ=255)
                                                                                           cAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCTGGGGGGGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACg                                             <  1:309309/151‑1 (MQ=255)
                                                                                            aGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCTGGGGGGGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGc                                            <  2:679500/151‑1 (MQ=255)
                                                                                               ttCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCTGGGGGGGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTAc                                         >  1:197176/1‑151 (MQ=255)
                                                                                                      aTTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCTGGGGGGGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATg                                  >  2:625061/1‑151 (MQ=255)
                                                                                                             aaGCCGGAAATTGATGGCAGCCGTCGTCTGGGGGGGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATc                           >  2:631810/1‑151 (MQ=255)
                                                                                                                    aaaTTGATGGCAGCCGTCGACTGGGGGGGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGAAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACATAAATGCCCGATCCtgg                       >  2:493289/1‑146 (MQ=255)
                                                                                                                          aTGGCAGCCGTCGTCTGGGGGGGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCAt              >  2:416319/1‑151 (MQ=255)
                                                                                                                             gCAGCCGTCGTCTGGGGGGGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCAtttt           <  2:629838/151‑1 (MQ=255)
                                                                                                                               aGCCGTCGTCTGGGGGGGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGa         <  1:535424/151‑1 (MQ=255)
                                                                                                                                  cgtcgtCTGGGGGGGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACtt      <  2:669299/151‑1 (MQ=255)
                                                                                                                                  cgtcgtCTGGGGGGGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCCAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCAGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGa         <  1:543090/148‑1 (MQ=255)
                                                                                                                                     cgtcTGGGGGGGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACtttt    <  2:456288/150‑1 (MQ=255)
                                                                                                                                      gtcTGGGGGGGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTAc  >  1:541627/1‑151 (MQ=255)
                                                                                                                                        cTGGGGGGGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTAc  >  1:9059/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                             
TGGTCCTGCAGCTTCTGCCGTTATTCTGCCACAACTGACCAGTCAGAATGTCACGTTTGATAATGTGCAGAATGCCGTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCTGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTAC  >  NC_000913/1931834‑1932118

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑