Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,810,756 G→T intergenic (+77/‑14) ygaH → / → mprA putative L‑valine exporter, norvaline resistance protein/transcriptional repressor of microcin B17 synthesis and multidrug efflux

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,810,7560GT100.0% 226.5 / NA 59intergenic (+77/‑14)ygaH/mprAputative L‑valine exporter, norvaline resistance protein/transcriptional repressor of microcin B17 synthesis and multidrug efflux
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (32/27);  total (32/27)

GCATTATCATCCCAACACTGCTTAGTGCGCTGGCCTATGGGCTCGCCTGGAAAGTGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCG  >  NC_000913/2810609‑2810900
                                                                                                                                                   |                                                                                                                                                
gcaTTATCATCCCAACACTGCTTAGTGCGCTAGCCTATGGGCTCGCCTGGAAAGTGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTGACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATTAgg                                                                                                                                               <  1:451902/151‑1 (MQ=255)
  aTTATCATCCCAACACTGCTTAGTGCGCTGGCCTATGGGCTCGCCTGGAAAGTGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATTAGGTc                                                                                                                                             >  2:457755/1‑151 (MQ=255)
        atcCCAACACTGCTTAGTGCGCTGGCCTATGGGCTCGCCTGGAAAGTGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATTAGGTCATAccc                                                                                                                                       >  1:173206/1‑151 (MQ=255)
          cccAACACTGCTTAGTGCGCTGGCCTATGGGCTCGCCTGGAAAGTGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATTAGGTCATACCCa                                                                                                                                      <  1:661850/150‑1 (MQ=255)
               cacTGCTTAGTGCGCTGGCCTATGGGCTCGCCTGGAAAGTGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATTAGGTCATACCCAAATgg                                                                                                                                 >  1:36608/1‑150 (MQ=255)
                      tAGTGCGCTGGCCTATGGGCTCGCCTGGAAAGTGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATTAGGTCATACCCAAATGGATAGtt                                                                                                                           <  2:294281/149‑1 (MQ=255)
                       aGTGCGCTGGCCTATGGGCTCGCCTGGAAAGTGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATTAGGTCATACCCAAATGGATAGTTCGt                                                                                                                        >  1:243122/1‑151 (MQ=255)
                       aGTGCGCTGGCCTATGGGCTCGCCTGGAAAGTGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATTAGGTCATACCCAAATGGATAGTTCGt                                                                                                                        <  1:706074/151‑1 (MQ=255)
                          gcgcTGGCCTATGGGCTCGCCTGGAAAGTGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATTAGGTCATACCCAAATGGATAGTTCGTTTa                                                                                                                     <  2:290708/151‑1 (MQ=255)
                               ggCCTATGGGCTCGCCTGGAAAGTGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATTAGGTCATACCCAAATGGATAGTTCGTTTACGccc                                                                                                                >  2:116438/1‑151 (MQ=255)
                                  cTATGGGCTCGCCTGGAAAGTGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATTAGGTCATACCCAAATGGATAGTTCGTTTACGCCCAtt                                                                                                             >  2:627929/1‑151 (MQ=255)
                                    aTGGGCTCGCCTGGAAAGTGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATTAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTg                                                                                                            <  2:243113/150‑1 (MQ=255)
                                    aTGGGCTCGCCTGGAAAGTGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATTAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTg                                                                                                            <  1:685782/150‑1 (MQ=255)
                                        gCTCGCCTGGAAAGTGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATTAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACa                                                                                                        >  2:610436/1‑150 (MQ=255)
                                             ccTGGAAAGTGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATTAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCt                                                                                                  <  1:627950/151‑1 (MQ=255)
                                                ggAAAGTGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATTAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTaa                                                                                                >  1:408206/1‑150 (MQ=255)
                                                 gAAAGTGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATTAGTTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTaaa                                                                                               >  2:104847/1‑150 (MQ=255)
                                                 gAAAGTGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATTAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTaaaa                                                                                              <  1:688459/151‑1 (MQ=255)
                                                       gATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATTAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcgc                                                                                        >  1:41810/1‑151 (MQ=255)
                                                           gCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATTAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCa                                                                                    <  2:207206/151‑1 (MQ=255)
                                                             gATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATTAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAgc                                                                                  >  2:228164/1‑151 (MQ=255)
                                                                 atataACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATTAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAgccgcc                                                                              >  1:278695/1‑151 (MQ=255)
                                                                   ataACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATTAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCa                                                                             <  1:29988/150‑1 (MQ=255)
                                                                     aaCCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATTAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACg                                                                           >  1:254695/1‑150 (MQ=255)
                                                                     aaCCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATTAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGa                                                                          <  1:12042/151‑1 (MQ=255)
                                                                           caAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATTAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGAttt                                                                    >  1:558986/1‑151 (MQ=255)
                                                                             aGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATTAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGAtttt                                                                   >  2:681677/1‑150 (MQ=255)
                                                                                aTCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATTAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCtt                                                               >  1:285619/1‑151 (MQ=255)
                                                                                  cATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATTAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTAt                                                             >  1:250282/1‑151 (MQ=255)
                                                                                     ttttCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATTAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATc                                                            >  1:729041/1‑149 (MQ=255)
                                                                                      tttCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATTAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAgg                                                         >  1:185263/1‑151 (MQ=255)
                                                                                         cTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATTAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAgg                                                         >  1:576921/1‑148 (MQ=255)
                                                                                           aaaaCAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATTAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAgg                                                         >  1:267588/1‑146 (MQ=255)
                                                                                           aaaaCAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATTAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGgaga                                                      <  2:41801/149‑1 (MQ=255)
                                                                                           aaaaCAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATTAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGgaga                                                      <  1:215513/149‑1 (MQ=255)
                                                                                           aaaaCAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATTAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGAt                                                     <  1:319639/150‑1 (MQ=255)
                                                                                               cAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATTAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCtt                                                 >  1:494998/1‑150 (MQ=255)
                                                                                               cAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATTAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTc                                                <  1:678028/151‑1 (MQ=255)
                                                                                                     aTTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATTAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTc                                          <  2:643578/151‑1 (MQ=255)
                                                                                                       ttACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATTAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGt                                        >  2:413755/1‑151 (MQ=255)
                                                                                                        tACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATTAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGt                                        <  1:260601/150‑1 (MQ=255)
                                                                                                        tACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATTAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGt                                        <  2:145494/150‑1 (MQ=255)
                                                                                                        tACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATTAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTc                                       >  1:476596/1‑151 (MQ=255)
                                                                                                           tttATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATTAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCttt                                    >  1:214598/1‑151 (MQ=255)
                                                                                                            ttATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATTAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCttt                                    <  1:362277/150‑1 (MQ=255)
                                                                                                              aTTTGTCACTGTCGTTACTATATCGGCTGAAATTAATTAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTtgca                                 <  1:291779/151‑1 (MQ=255)
                                                                                                                 tGTCACTGTCGTTACTATATCGGCTGAAATTAATTAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTtgcatgc                              >  1:726210/1‑151 (MQ=255)
                                                                                                                    cACTGTCGTTACTATATCGGCTGAAATTAATTAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGcatgcaca                           <  2:111672/151‑1 (MQ=255)
                                                                                                                          cGTTACTATATCGGCTGAAATTAATTAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCaa                      >  1:652719/1‑150 (MQ=255)
                                                                                                                          cGTTACTATATCGGCTGAAATTAATTAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCaa                      <  1:638547/150‑1 (MQ=255)
                                                                                                                          cGTTACTATATCGGCTGAAATTAATTAGGTCATACCCAAATGGATAGCTCGTTTACGCCCATTGAACAAATGCTATAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCaa                      >  1:534814/1‑150 (MQ=255)
                                                                                                                              aCTATATCGGCTGAAATTAATTAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAaagca                  >  2:213708/1‑150 (MQ=255)
                                                                                                                              aCTATATCGGCTGAAATTAATTAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAaagca                  >  2:328040/1‑150 (MQ=255)
                                                                                                                              aCTATATCGGCTGAAATTAATTAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAaagca                  <  1:442351/150‑1 (MQ=255)
                                                                                                                                 atatCGGCTGAAATTAATTAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAgct              >  2:465113/1‑151 (MQ=255)
                                                                                                                                   atCGGCTGAAATTAATTAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAgctg             <  1:228172/150‑1 (MQ=255)
                                                                                                                                      ggCTGAAATTAATTAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTgg         <  2:208667/151‑1 (MQ=255)
                                                                                                                                       gCTGAAATTAATTAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGga        <  1:299755/151‑1 (MQ=255)
                                                                                                                                            aattaattAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAAcc   >  1:295464/1‑151 (MQ=255)
                                                                                                                                               tAATTAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCTTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCg  >  1:291559/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
GCATTATCATCCCAACACTGCTTAGTGCGCTGGCCTATGGGCTCGCCTGGAAAGTGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCG  >  NC_000913/2810609‑2810900

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: