Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,206,435	0	C	T	40.7%	63.2 / 26.9	54	intergenic (‑179/‑28)	ttdR/ttdA	transcriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (7/25);  new base T (22/0);  total (29/25)
Fisher's exact test for biased strand distribution p-value = 2.47e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAG‑TGAATAAG‑‑TTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCT  >  NC_000913/3206287‑3206581                                                                                                                                                     |                                                                                                                                                      gtgtATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCa                                                                                                                                                     <  1:450203/151‑1 (MQ=255)    tATTCCGCAAAACATTGCCATTCCCTGCTCTAGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAACAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCaca                                                                                                                                                   >  1:79368/1‑148 (MQ=255)      tttCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAg                                                                                                                                                    >  2:449782/1‑147 (MQ=255)           cAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACa                                                                                                                                           <  2:475214/151‑1 (MQ=255)             aaaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATATTCGCGTTGATTCGCGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACaaa                                                                                                                                         <  1:119392/151‑1 (MQ=255)              aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAaaaaaaaaaaa                                                                                                                                          >  1:59755/1‑144 (MQ=255)                   tGCCATACCCTGCTCTGGTGCAATATTCATTCTTATTTTCTCGTTGATTCACGAACTGTTAAATGCAGAATCGCGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGAAAAATACAAAAACTg                                                                                                                                    <  1:509368/150‑1 (MQ=255)                    gCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTgg                                                                                                                                   <  2:457557/150‑1 (MQ=255)                      cATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAACAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGTAATTTTCACGAGAAAAACaaaaaatt                                                                                                                                    >  1:762514/1‑144 (MQ=255)                       aTTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATAAAAAAAATGGAGtt                                                                                                                               >  2:122163/1‑151 (MQ=255)                         tCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTg                                                                                                                              <  2:755196/150‑1 (MQ=255)                         tCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCCGACAAAAACAAAAAATGGGGtt                                                                                                                               >  2:391231/1‑149 (MQ=255)                              gCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCat                                                                                                                          <  1:510636/149‑1 (MQ=255)                              gCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCat                                                                                                                          <  2:710230/149‑1 (MQ=255)                               ctctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGAATTTTTTTAGAAAAAAAAAAAAAATAGAGt                                                                                                                                >  1:634166/1‑142 (MQ=255)                                   ggCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGc                                                                                                                   <  1:787344/151‑1 (MQ=255)                                    gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAACTGGAGTTGGCATGATTAGCg                                                                                                                  >  2:492570/1‑151 (MQ=255)                                      gcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCATACAAATACAAAAAAAGGAGTTGCCATGATGAGCg                                                                                                                  >  1:624833/1‑149 (MQ=255)                                        aaTATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAg                                                                                                              <  2:675448/151‑1 (MQ=255)                                         atatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGAAAAAAACAAAAAAAGGAGTTGCCATGATGGGCGaaa                                                                                                               >  2:610908/1‑149 (MQ=255)                                          tatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTATATTTTTTCACGCAAAAACAAAAAAAGGGAGTGCCATGATGATCGgaa                                                                                                               >  2:313577/1‑148 (MQ=255)                                             tcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAAATTGAGTTGTCCTGTTGAGCGGAAGtaa                                                                                                           >  2:314086/1‑149 (MQ=255)                                             tcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGtaat                                                                                                          <  2:96084/150‑1 (MQ=255)                                             tcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGtaat                                                                                                          <  2:237277/150‑1 (MQ=255)                                                ttcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCAACAAAAACAAAAACTGGCGTTGGCATGATGAGCGGAAGcaataa                                                                                                        >  2:363896/1‑149 (MQ=255)                                                       tttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAAATTGAGTTGTCATGATGAGGGGAAGaaataaaaaaaa                                                                                                  >  2:564887/1‑142 (MQ=255)                                                         tCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCa                                                                                              <  2:236012/150‑1 (MQ=255)                                                               tGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATACAAAAAATGGAGGTGCCCTGATGAGCGAAAGAAATAAACAACAAGCAG‑TGaa                                                                                        >  2:177190/1‑149 (MQ=255)                                                                 aTTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAG‑TGAATAAg                                                                                    <  1:409459/151‑1 (MQ=255)                                                                   tCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGAGAATTTTTTCAGAACAATACCAAAAAAAGGGTTTCCATTGTGAGGGGAAGTAATAAACAATAGGGAG‑CGAATa                                                                                      >  1:41445/1‑147 (MQ=255)                                                                                 ttACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTCTACACAAAAACAAAAAACGGAGTTGCCCAGGGGTGCGGAAGTAAAAAAGAAAAAGCAA‑GGAAAAAA‑‑TTGACCgagagtttt                                                                   >  2:504636/1‑145 (MQ=255)                                                                                     atatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAAATGGAGTTGCCATGATGAGCGGAAGGAATAAACAAAAAGGAG‑GGAAAAAG‑‑TTGACAGAGATTGTTGCTa                                                               >  2:747708/1‑150 (MQ=255)                                                                                     atatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAG‑TGAATAAG‑‑TTGACAGAGATTGTCGCTaa                                                              <  2:323932/151‑1 (MQ=255)                                                                                          cACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAG‑TGAATAAG‑‑TTGACAGAGATTGTCGCTAACttt                                                          <  1:692638/150‑1 (MQ=255)                                                                                           tcGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAAAAAAATAAAAAAAATGTAGTTGCCATTAAGGGGGAAAACAATAAACAAAAACCAC‑GGAATAAA‑‑ATGTCAGAGATTGTGGCTCACctt                                                          >  1:205666/2‑149 (MQ=255)                                                                                           aCGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGAAAAATAAAAAAAATAGAGGTGGCCTGATGAGGGGAAGGAATAAAGAAAAGGCAG‑GGAATAAA‑‑GTGACAGAGATTGTCGCCAACttt                                                          >  2:575627/1‑149 (MQ=255)                                                                                             ggTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAG‑TGAATAAG‑‑TTGACAGAGATTGTCGCTAACtt                                                           <  1:489471/146‑1 (MQ=255)                                                                                             ggTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAG‑TGAATAAG‑‑TTGACAGAGATTGTCGCTAACTTTACCg                                                      <  2:369653/151‑1 (MQ=255)                                                                                             ggTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAG‑TGAATAAG‑‑TTGACAGAGATTGTCGCTAACTTTACCg                                                      <  2:624892/151‑1 (MQ=255)                                                                                                   aaaaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTTCAAAAAAAACAAAAAATGGGGTTGGCCTGATGAGGGGAAGGAAAAAACAACAAGCAG‑GGAATAAA‑‑ATTACAGAGAATGTTGCTAACATTACCGcc                                                    >  1:466721/1‑147 (MQ=255)                                                                                                          ataAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCAAAACAAAACAAAAAATGGAGTTGGCATGATGGGGGGGAGTAATAAAAAAAAAGGAGATGAAAAAA‑‑TT‑ACACAAATTGTTGCTAAATTTAACGCCCTGAttt                                             >  1:181746/1‑147 (MQ=255)                                                                                                          ataAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGAATTTTTCTGCCAAAAACAAAAACTTGGGTTGCCATGATTGGCGAAAGTAATAAAAAACATCAAG‑TGAAATAG‑‑CTGCCAGTGAATGTTGATAACTTTACCCCCTTGATTTCttc                                         >  2:476748/1‑149 (MQ=255)                                                                                                               aCCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCAACAAAAAAAAAAACACGGGTTCGCGTTATGAGCCGAAGAAATAAACAAAAAGCAA‑TGATTAAGGTTTAACAGA‑ATCATC‑CGAACATTTCCGCCAAGATTTCTAccc                                       >  2:207435/1‑148 (MQ=255)                                                                                                                     tttttAGTGGTAGTTTGGGGCGATAAATTTTTCAAGAAAAATACAAAAACTGGAGTTGCCATGATGAGCAAAATAAATAAGCAACAGGCAG‑TAAATAAG‑‑TTGACAGAGATTGTCGATACTTTTACCGCCATGATTTCTACCCGaa                                    <  1:633606/145‑1 (MQ=255)                                                                                                                      ttttAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGACAAAAAAAAAAAATGGAGTTGCCATGGTGAGGGGAAAGAATAAACAAAAGGCAG‑TGAATAAA‑‑TTGACAGAGATTGTCGCCAAATTTACCCCCATGATTTTTAACCCAAAGcc                                >  2:391651/1‑148 (MQ=255)                                                                                                                             ggTAGTTTGTGGCGGTGAATTTTTCCAGAAAAATACAAAAACGGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAT‑TAAATAAG‑‑TTAACGGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgtg                      <  1:96714/151‑1 (MQ=255)                                                                                                                                aGTTTGTGGTGTTAATTTTTTCCAGACTAAAACAAAATCGGGATTTGCCAGGGTGAGTGAAAGTAAGAAGCAACAGCAAA‑TCAATACA‑‑TGAACAGAGGTTTTACTAAAGTTCACCGCCATGATTTCTACCCGTATGCCtg                              <  1:342478/140‑1 (MQ=255)                                                                                                                                       ggCGGTGAATTTTTCAAGAAAAAAACAAAAACTGGATTTCCCATGATGAGCAAAATTAATAAGCACCAGGCAG‑TAAATAAG‑‑TTGACAGATATTGTCGCGATCTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATaa              <  1:45719/149‑1 (MQ=255)                                                                                                                                            tGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAG‑TGAATAAG‑‑TTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaa        >  2:379290/1‑150 (MQ=255)                                                                                                                                             gAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAG‑TGAATAAG‑‑TTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAAc      >  2:447098/1‑151 (MQ=255)                                                                                                                                              aaTTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAG‑TGAATAAG‑‑TTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAAc      <  2:62695/150‑1 (MQ=255)                                                                                                                                              aaTTTTTCCAGAAAAATAAAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAG‑TGAATAAG‑‑TTGACAGAGATTGTCGTTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACa     <  2:618167/151‑1 (MQ=255)                                                                                                                                               aTTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAG‑TGAATAAG‑‑TTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAAc      >  1:381464/1‑149 (MQ=255)                                                                                                                                                  tttCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAG‑TGAATAAG‑‑TTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCt  >  1:267090/1‑150 (MQ=255)                                                                                                                                                     |                                                                                                                                                      GTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAG‑TGAATAAG‑‑TTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCT  >  NC_000913/3206287‑3206581

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑