Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,220,759	0	C	A	38.9%	55.1 / 17.3	36	A422A (GCC→GCA)	patA	putrescine:2‑oxoglutaric acid aminotransferase, PLP‑dependent
Reads supporting (aligned to +/- strand):  ref base C (15/7);  new base A (1/13);  total (16/20)
Fisher's exact test for biased strand distribution p-value = 4.31e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTAAAGGGATGTTGATGGCGATTGAGTTTGTTGATAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAGGCGCTGGCGGCCATGCGAGTAAGTGTCGAAGAAGCGTAATATCAAATCGGATGGCGATACGACGTC  >  NC_000913/3220615‑3220900                                                                                                                                                 |                                                                                                                                              gcgGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTAAAGGGATGTTGATGGCGATTGAGTTTGTTGATAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACg                                                                                                                                         <  1:257919/151‑1 (MQ=255)       atatCCCGATCTGGTACAGGAAGCGCGTGGTAAAGGGATGTTGATGGCGATTGAGTTTGTTGATAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGc                                                                                                                                   >  2:694961/1‑151 (MQ=255)       atatCCCGATCTGGTACAGGAAGCGCGTGGTAAAGGGATGTTGATGGCGATTGAGTTTGTTGATAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGc                                                                                                                                   <  1:379645/151‑1 (MQ=255)         atCCCGATCTGGTACAGGAAGCGCGTGGTAAAGGGATGTTGATGGCGATTGAGTTTGTTGATAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCAt                                                                                                                                 <  1:267229/151‑1 (MQ=255)           cccGATCTGGTACAGGAAGCGCGTGGTAAAGGGATGTTGATGGCGATTGAGTTTGTTGATAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCAtt                                                                                                                                <  2:153708/150‑1 (MQ=255)                   ggTACAGGAAGCGCGTGGTAAAGGGATGTTGATGGCGATTGAGTTTGTTGATAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGa                                                                                                                              <  1:140048/144‑1 (MQ=255)                   ggTACAGGAAGCGCGTGGTAAAGGGATGTTGATGGCGATTGAGTTTGTTGATAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAAc                                                                                                                            <  2:348680/146‑1 (MQ=255)                               gcgTGGTAAAGGGATGTTGATGGCGATTGAGTTTGTTGATAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGAcc                                                                                                           >  1:601452/1‑151 (MQ=255)                                     tAAAGGGATGTTGATGGCGATTGAGTTTGTTGATAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGa                                                                                                      >  2:320310/1‑150 (MQ=255)                                      aaaGGGTTGTTGATGGGGTTTGATTTTGTTGAAAACAAAATGGGTTATAATTTGGCCAGGGAGTTGTCCCGCCGGCGGTTAGGGGTGGCGGAAACGCTAAAAACCCCAAAAACGACCCGCTTTAAACCGCCACTGACACTGACCATTGa                                                                                                      <  2:740886/149‑1 (MQ=255)                                          gtaTGTTGTGGGCGTTTGTGTTTTTTAAAACCAAAATCGTTTATATTTTTGCCAGGGAGATGTCCCGCCAGCGCGTTCTGGTGGCCGGAACGCTAAAAAACCCAAAAACGACCCGCTTTAAACCGCCACTGACACTGACCATTGAACAgtg                                                                                                <  2:231671/149‑1 (MQ=255)                                             tGTTGAGGGCGATGTATTTTTTGGAAAACAAAACCGGTTATACTTTGGCCAGCGAGTTGTCCCGCCAGCGGTTCCGGGTGCCGGGACCCCTCAAAACCGCAAAAACGCCCCGCTTTGAACCGCCACTGACACTGACCATTGAACAgtg                                                                                                <  2:96387/148‑1 (MQ=255)                                              gTTGAGGTCGATTGATTTTGTTGATAACAAAATCGGTTATACCTTTCCCAGGGAGATTTCCCGCGAGCGCGTACTGGGGGCCGGACCGCTAAAAAACGAAAAAACGACCCGCTTTGAACCGCCACTGACACTGACCATTGAACAgtgt                                                                                               <  2:677591/148‑1 (MQ=255)                                               ttGATGGCGATTGAGTTTGTTGATAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAAc                                                                                           >  2:133417/1‑151 (MQ=255)                                                       gATTGAGTTTGTTGATAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATc                                                                                   >  2:232442/1‑151 (MQ=255)                                                              tttgtgaaaaaCAAAATCGGTTATAATTTTGCCAGCGAGATGTCCCGCCGGCGCGTCCGGGGGGCCGAAACCCTAAAAAACGCAAAAACGACCCGGATGAAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCa                                                                                  <  1:320271/136‑1 (MQ=255)                                                                  ttgATAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGcgcg                                                                        >  2:381190/1‑151 (MQ=255)                                                                   tgATAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGt                                                                       >  1:128952/1‑151 (MQ=255)                                                                           aaaTCGGTTATAACTTTGCCAGGGAGATTTCCCGCCAGCGCGTCGGGGGGGCCGGACCGTTAAAAAACGCAAAACCGACCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAGgc                                                                  <  2:601509/148‑1 (MQ=255)                                                                                         tttGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAGGCGCTGGCGGCCATGCg                                                   >  1:392567/1‑149 (MQ=255)                                                                                           tGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAGGCGCTGGCGGCCATGCGAg                                                 >  1:562360/1‑149 (MQ=255)                                                                                                gCGAGTTGTCCCGCCAGCGCGTACGGGTGGCGGGAACGCTCAAAAACGCAAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAGGCGCTGGCGGCCATGCGAGt                                                <  2:324569/145‑1 (MQ=255)                                                                                                  gagaTGTTCCGCCGGCGAGTCCTGGTGGCCGAACCGCCAAATACCCAAAAACCGCCCCGCATCGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAGGCGCTGGCGGCCATGCGAGTAAGTGTCg                                        <  1:717201/151‑1 (MQ=255)                                                                                                    gaTGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAGGCGCTGGCGGCCATGCGAGTAAGTGTc                                         >  1:169102/1‑148 (MQ=255)                                                                                                     aTGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAGGCGCTGGCGGCCATGCGAGTAAGTGTCga                                       >  1:39169/1‑149 (MQ=255)                                                                                                        tcccgccgGCGCTTTCTGTTGGCCGGAACGCTCAAAACCCCAAAAACGTCCCGTTTTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAGGCGCTGGCGGCCATGCGAGTAAGTGTCg                                        <  2:114273/143‑1 (MQ=255)                                                                                                          cccccAGCGCTTACTGGGGGCGGAAACGCAAAAGAGCGCCAAAACGACCCCCTTTAAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAGGCGCTGGCGGCCATGCGAGTAAGTGTCGAAGAAGCg                                <  1:487661/148‑1 (MQ=255)                                                                                                           cGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACAATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAGGCGCTGGCGGCCATGCGAGTAAGTGTCGAAGAAGCGt                               >  1:606115/1‑151 (MQ=255)                                                                                                            gCCAGCGCGTACTGGTAGACGGAACGCTCAATAACGCAAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGCCGCGTAAGGCGCTGGCGGCCAAGCGAATAAGTCTCCAAGAAGCGt                               >  1:386819/1‑150 (MQ=255)                                                                                                                 cgcgTACTGGTGGCCGGAACGCTAAAAACCGCAAAAACGCCCCGAATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAGGCGCTGGCGGCCATGCGAGTAAGTGTCGAAGAAGCGTaa                             <  2:195153/147‑1 (MQ=255)                                                                                                                  tcgcaCTGGCAGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACGGCCACTTACACTGACCATTGAACAGTGTGATCTGGGGATCAAAGCGGCGCGTAAGGCGCTGGCGGCCTTTCGAGTCCGGGTCGACGAAGGGTGatat                          >  1:480028/5‑149 (MQ=255)                                                                                                                         ggtggCCGGAACGCTCAATACCGCAAAAACGACCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAGGCGCTGGCGGCCATGCGAGTAAGTGTCGAAGAAGCGTAATATCAAATc                    <  2:501913/148‑1 (MQ=255)                                                                                                                           tggCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCTTTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAGGCGCTGGCGGCCATGCGAGTAAGTGTCGAAGAAGCGTAATATCAAATCGGATg               >  1:269644/1‑151 (MQ=255)                                                                                                                            ggCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAGGCGCTGGCGGCCATGCGAGTAAGTGTCGAAGAAGCGTAATATCAAATCGGATgg              >  2:465110/1‑151 (MQ=255)                                                                                                                                  accGCTAAATACCGCAAAAAGGACCCGAATGAAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAGGCGCTGGCGGCCATGCGAGTAAGTGTCGAAGAAGCGTAATATCAAATCGGATGGCg            <  2:807112/145‑1 (MQ=255)                                                                                                                                         aacaaCGCAAAACCGCCCCGCATGAAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAGGCGCTGGCGGCCATGCGAGTAAGTGTCGAAGAAGCGTAATATCAAATCGGATGGCGATACGACGTc  <  1:416707/147‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                              GCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTAAAGGGATGTTGATGGCGATTGAGTTTGTTGATAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAGGCGCTGGCGGCCATGCGAGTAAGTGTCGAAGAAGCGTAATATCAAATCGGATGGCGATACGACGTC  >  NC_000913/3220615‑3220900

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑