Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,220,7670TC38.5% 50.1 / 13.8 39I425T (ATC→ACC) patAputrescine:2‑oxoglutaric acid aminotransferase, PLP‑dependent
Reads supporting (aligned to +/- strand):  ref base T (17/7);  new base C (0/15);  total (17/22)
Fisher's exact test for biased strand distribution p-value = 7.38e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATATCCCGATCTGGTACAGGAAGCGCGTGGTAAAGGGATGTTGATGGCGATTGAGTTTGTTGATAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAGGCGCTGGCGGCCATGCGAGTAAGTGTCGAAGAAGCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTT  >  NC_000913/3220621‑3220914
                                                                                                                                                  |                                                                                                                                                   
atatCCCGATCTGGTACAGGAAGCGCGTGGTAAAGGGATGTTGATGGCGATTGAGTTTGTTGATAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGc                                                                                                                                                 >  2:694961/1‑151 (MQ=255)
atatCCCGATCTGGTACAGGAAGCGCGTGGTAAAGGGATGTTGATGGCGATTGAGTTTGTTGATAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGc                                                                                                                                                 <  1:379645/151‑1 (MQ=255)
  atCCCGATCTGGTACAGGAAGCGCGTGGTAAAGGGATGTTGATGGCGATTGAGTTTGTTGATAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCAt                                                                                                                                               <  1:267229/151‑1 (MQ=255)
    cccGATCTGGTACAGGAAGCGCGTGGTAAAGGGATGTTGATGGCGATTGAGTTTGTTGATAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCAtt                                                                                                                                              <  2:153708/150‑1 (MQ=255)
            ggTACAGGAAGCGCGTGGTAAAGGGATGTTGATGGCGATTGAGTTTGTTGATAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGa                                                                                                                                            <  1:140048/144‑1 (MQ=255)
            ggTACAGGAAGCGCGTGGTAAAGGGATGTTGATGGCGATTGAGTTTGTTGATAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAAc                                                                                                                                          <  2:348680/146‑1 (MQ=255)
                        gcgTGGTAAAGGGATGTTGATGGCGATTGAGTTTGTTGATAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGAcc                                                                                                                         >  1:601452/1‑151 (MQ=255)
                              tAAAGGGATGTTGATGGCGATTGAGTTTGTTGATAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGa                                                                                                                    >  2:320310/1‑150 (MQ=255)
                               aaaGGGTTGTTGATGGGGTTTGATTTTGTTGAAAACAAAATGGGTTATAATTTGGCCAGGGAGTTGTCCCGCCGGCGGTTAGGGGTGGCGGAAACGCTAAAAACCCCAAAAACGACCCGCTTTAAACCGCCACTGACACTGACCATTGa                                                                                                                    <  2:740886/149‑1 (MQ=255)
                                   gtaTGTTGTGGGCGTTTGTGTTTTTTAAAACCAAAATCGTTTATATTTTTGCCAGGGAGATGTCCCGCCAGCGCGTTCTGGTGGCCGGAACGCTAAAAAACCCAAAAACGACCCGCTTTAAACCGCCACTGACACTGACCATTGAACAgtg                                                                                                              <  2:231671/149‑1 (MQ=255)
                                      tGTTGAGGGCGATGTATTTTTTGGAAAACAAAACCGGTTATACTTTGGCCAGCGAGTTGTCCCGCCAGCGGTTCCGGGTGCCGGGACCCCTCAAAACCGCAAAAACGCCCCGCTTTGAACCGCCACTGACACTGACCATTGAACAgtg                                                                                                              <  2:96387/148‑1 (MQ=255)
                                       gTTGAGGTCGATTGATTTTGTTGATAACAAAATCGGTTATACCTTTCCCAGGGAGATTTCCCGCGAGCGCGTACTGGGGGCCGGACCGCTAAAAAACGAAAAAACGACCCGCTTTGAACCGCCACTGACACTGACCATTGAACAgtgt                                                                                                             <  2:677591/148‑1 (MQ=255)
                                        ttGATGGCGATTGAGTTTGTTGATAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAAc                                                                                                         >  2:133417/1‑151 (MQ=255)
                                                gATTGAGTTTGTTGATAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATc                                                                                                 >  2:232442/1‑151 (MQ=255)
                                                       tttgtgaaaaaCAAAATCGGTTATAATTTTGCCAGCGAGATGTCCCGCCGGCGCGTCCGGGGGGCCGAAACCCTAAAAAACGCAAAAACGACCCGGATGAAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCa                                                                                                <  1:320271/136‑1 (MQ=255)
                                                           ttgATAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGcgcg                                                                                      >  2:381190/1‑151 (MQ=255)
                                                            tgATAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGt                                                                                     >  1:128952/1‑151 (MQ=255)
                                                                    aaaTCGGTTATAACTTTGCCAGGGAGATTTCCCGCCAGCGCGTCGGGGGGGCCGGACCGTTAAAAAACGCAAAACCGACCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAGgc                                                                                <  2:601509/148‑1 (MQ=255)
                                                                                  tttGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAGGCGCTGGCGGCCATGCg                                                                 >  1:392567/1‑149 (MQ=255)
                                                                                    tGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAGGCGCTGGCGGCCATGCGAg                                                               >  1:562360/1‑149 (MQ=255)
                                                                                         gCGAGTTGTCCCGCCAGCGCGTACGGGTGGCGGGAACGCTCAAAAACGCAAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAGGCGCTGGCGGCCATGCGAGt                                                              <  2:324569/145‑1 (MQ=255)
                                                                                           gagaTGTTCCGCCGGCGAGTCCTGGTGGCCGAACCGCCAAATACCCAAAAACCGCCCCGCATCGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAGGCGCTGGCGGCCATGCGAGTAAGTGTCg                                                      <  1:717201/151‑1 (MQ=255)
                                                                                             gaTGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAGGCGCTGGCGGCCATGCGAGTAAGTGTc                                                       >  1:169102/1‑148 (MQ=255)
                                                                                              aTGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAGGCGCTGGCGGCCATGCGAGTAAGTGTCga                                                     >  1:39169/1‑149 (MQ=255)
                                                                                                 tcccgccgGCGCTTTCTGTTGGCCGGAACGCTCAAAACCCCAAAAACGTCCCGTTTTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAGGCGCTGGCGGCCATGCGAGTAAGTGTCg                                                      <  2:114273/143‑1 (MQ=255)
                                                                                                   cccccAGCGCTTACTGGGGGCGGAAACGCAAAAGAGCGCCAAAACGACCCCCTTTAAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAGGCGCTGGCGGCCATGCGAGTAAGTGTCGAAGAAGCg                                              <  1:487661/148‑1 (MQ=255)
                                                                                                    cGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACAATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAGGCGCTGGCGGCCATGCGAGTAAGTGTCGAAGAAGCGt                                             >  1:606115/1‑151 (MQ=255)
                                                                                                     gCCAGCGCGTACTGGTAGACGGAACGCTCAATAACGCAAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGCCGCGTAAGGCGCTGGCGGCCAAGCGAATAAGTCTCCAAGAAGCGt                                             >  1:386819/1‑150 (MQ=255)
                                                                                                          cgcgTACTGGTGGCCGGAACGCTAAAAACCGCAAAAACGCCCCGAATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAGGCGCTGGCGGCCATGCGAGTAAGTGTCGAAGAAGCGTaa                                           <  2:195153/147‑1 (MQ=255)
                                                                                                           tcgcaCTGGCAGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACGGCCACTTACACTGACCATTGAACAGTGTGATCTGGGGATCAAAGCGGCGCGTAAGGCGCTGGCGGCCTTTCGAGTCCGGGTCGACGAAGGGTGatat                                        >  1:480028/5‑149 (MQ=255)
                                                                                                                  ggtggCCGGAACGCTCAATACCGCAAAAACGACCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAGGCGCTGGCGGCCATGCGAGTAAGTGTCGAAGAAGCGTAATATCAAATc                                  <  2:501913/148‑1 (MQ=255)
                                                                                                                    tggCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCTTTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAGGCGCTGGCGGCCATGCGAGTAAGTGTCGAAGAAGCGTAATATCAAATCGGATg                             >  1:269644/1‑151 (MQ=255)
                                                                                                                     ggCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAGGCGCTGGCGGCCATGCGAGTAAGTGTCGAAGAAGCGTAATATCAAATCGGATgg                            >  2:465110/1‑151 (MQ=255)
                                                                                                                           accGCTAAATACCGCAAAAAGGACCCGAATGAAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAGGCGCTGGCGGCCATGCGAGTAAGTGTCGAAGAAGCGTAATATCAAATCGGATGGCg                          <  2:807112/145‑1 (MQ=255)
                                                                                                                              cctaaaaaccacaaaaaCGCCCCTCATGCAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAGGCGCTGGCGGCCATGCGAGTAAGTGTCGAAGAAGCGTAATATCAAATCGGATGGCGATacgac                   <  2:692324/138‑1 (MQ=255)
                                                                                                                                  aacaaCGCAAAACCGCCCCGCATGAAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAGGCGCTGGCGGCCATGCGAGTAAGTGTCGAAGAAGCGTAATATCAAATCGGATGGCGATACGACGTc                <  1:416707/147‑1 (MQ=255)
                                                                                                                                   aaaacccaaaaaCGATCGGAATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAGGCGCTGGCGGCCATGCGAGTAAGTGTCGAAGAAGCGTAATATCAAATCGGATGGCGATACGACGTCg               <  2:347251/142‑1 (MQ=255)
                                                                                                                                           aaaaCGACCCGCTTTAAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAGGCGCTGGCGGCCATGCGAGTAAGTGTCGAAGAAGCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGAt      <  2:619458/151‑1 (MQ=255)
                                                                                                                                               cGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAGGCGCTGGCGGCCATGCGAGTAAGTGTCGAAGAAGCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCttt  >  1:678284/1‑151 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
ATATCCCGATCTGGTACAGGAAGCGCGTGGTAAAGGGATGTTGATGGCGATTGAGTTTGTTGATAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAGGCGCTGGCGGCCATGCGAGTAAGTGTCGAAGAAGCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTT  >  NC_000913/3220621‑3220914

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑