Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,245,234	0	G	T	46.3%	38.1 / 21.8	41	L25F (TTG→TTT)	ycgY	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (7/15);  new base T (19/0);  total (26/15)
Fisher's exact test for biased strand distribution p-value = 2.75e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATCCGATAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATT  >  NC_000913/1245091‑1245384                                                                                                                                                |                                                                                                                                                       atCCGATAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTgcgc                                                                                                                                                 <  1:133657/151‑1 (MQ=255)    cGATAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTa                                                                                                                                              <  2:19050/151‑1 (MQ=255)     gATAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTAt                                                                                                                                             <  2:276898/151‑1 (MQ=255)          aGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTTTTTaa                                                                                                                                         >  2:561409/1‑150 (MQ=255)               gTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGGTGCGCTTATTGAACAttt                                                                                                                                    >  2:495504/1‑150 (MQ=255)               gTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTa                                                                                                                                   <  2:483517/151‑1 (MQ=255)                 tCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAg                                                                                                                                  <  2:427553/150‑1 (MQ=255)                       tAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGGTGCGCTTATTTAAAATTTAGa                                                                                                                                 >  2:118339/1‑145 (MQ=255)                        accaacaacaGAATCCTTTTTTAATTATTGCTTCGCTGCTTTTGGTGTGATTCAGAACATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGGTTTTGTTGAGCCTTTTCAACAATTATaaa                                                                                                                               >  1:90046/3‑145 (MQ=255)                                  gAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGGTGCGCCTTTTGAACATTTTGAATTGATCAATCCctct                                                                                                                 >  2:113781/1‑150 (MQ=255)                                   aaTCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGCTGCGGTTATTGAACATTTTGAAATGATCAATACTTCt                                                                                                                 >  1:769729/1‑149 (MQ=255)                                           tttAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGGGGCGCTTTTTGTACATTTTGAAATGATTAATCCTTCTGGGAGAtt                                                                                                         >  2:451710/1‑149 (MQ=255)                                             tAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAg                                                                                                      <  2:227774/150‑1 (MQ=255)                                                     gTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTTTTGAAAAATTATAATTGATCAATCCTTTTGGCAGATTAAGACCAAATa                                                                                              >  2:347466/1‑150 (MQ=255)                                                      tttCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTTAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCcaaaaaac                                                                                            >  2:251610/1‑147 (MQ=255)                                                                 ttGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAAAACCCGTTCGGCaa                                                                                  >  2:364167/1‑150 (MQ=255)                                                                  tGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGATTTTGCATCGCCTTTTTAACAATTATAAATGAACAAACCTTCTGGCTGATTTAGCCCAAATAACCGTTCgg                                                                                     >  1:578149/1‑146 (MQ=255)                                                                    gtgtGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAAAAACCGTTCGGCaa                                                                                  >  2:279572/1‑147 (MQ=255)                                                                      gtgATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCtt                                                                             <  1:130959/150‑1 (MQ=255)                                                                         aTTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGCGGCGCTTATTTAAAATTTAGAATTGATTAAACCTTCTTGCAGATTAAACCCAAATACCCGGTCGGCAAAGCTTCAGt                                                                         >  1:178190/1‑151 (MQ=255)                                                                          ttCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGtt                                                                        >  2:147779/1‑151 (MQ=255)                                                                                          tttAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGCTGCGCCTATTGAACATTTAGAATTTATCAAACCTTCTTGCCGAATAAGCCAAAATAACCCTTCGGCAATGCTTCAGGTACGCGAAGAGGaaatg                                                        >  2:436120/1‑148 (MQ=255)                                                                                             aGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTc                                                      <  2:539887/150‑1 (MQ=255)                                                                                             aGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCt                                                     <  2:201755/151‑1 (MQ=255)                                                                                              gACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGCTGCGCTTATTGAAAATTTAGAATTGGTCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGt                                                       >  2:507669/1‑148 (MQ=255)                                                                                               aCAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGGTTTGGCTGCGCTTTTTTAACATTTAGAATTGATCAATCCTTCTGGCAGGTTAAACCAAAAAAACCGTTCGGCAATGCTTCAATTAAGCGAAGAGGGATGGTCt                                                     >  2:422870/1‑149 (MQ=255)                                                                                                cAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGCTGCGCTTATTGAAAATTTAGAAATGATCAATCCTTCTTGCAGATTAAACCCAAAAACCCCGTCGGGCATGCTTTAGTTACGCGGAGAGGGATgg                                                        >  2:336563/1‑145 (MQ=255)                                                                                                   tAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGGTGCGGTTATTGAACAATTAGAATTGGTCCAACCTTCTTGGGGGGTAAGCCCAAATAACCGGTCAGGAATGCTTCAGTTTAGCGAAGAGGGAAGGGCTGGAtt                                                >  2:242519/1‑150 (MQ=255)                                                                                                    aGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAAtgc                                               <  1:3051/150‑3 (MQ=255)                                                                                                       cAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCtttt                                           <  1:632565/151‑1 (MQ=255)                                                                                                       cAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCtttt                                           <  1:533436/151‑1 (MQ=255)                                                                                                          tCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGGCGCGCCTTTTTAAAAATTAGAATTTATCAATCCTTCTTGGCGATTAAGCCCAAAAAACCCTTCGGCAATGCTTCAGTTTAGCGGAGAGGGAAGGGCTGAATTCttttttt                                        >  1:372434/1‑151 (MQ=255)                                                                                                          tCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGCTTCGGTTATTTAACATTTTGAAATGATTAATCCTTCTGGCAGATTAAGCCAAAAAACCCGTTCGGGAAAGCTTCAGTTTCGCGAAGGGGAATGGTCTGAATTCttttttt                                        >  1:82175/1‑151 (MQ=255)                                                                                                           cAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCttttttt                                        <  2:754890/150‑1 (MQ=255)                                                                                                                gTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGCTGTGATTATTGAAAACTTATAATTGATCTCTACTTCTGGCAGATTTAGCCAACAAACCCCCTTGGGAATGCCTCAGTTTCGCGAAGCGGCATAGTCTGAATTCTTTTTTTTGtt                                    >  1:794399/1‑149 (MQ=255)                                                                                                                   aaaaaTGGGGCGGATAGTGAGGCGGTTTTTGCTGCGCTTATTGAAAATTTTGAATTGATCAAACCTTCTGGCAGATTAAGCCAAAATACCCGGTCGGCAATGCTTCAGTTACGCGAAGAGGGATGGTCTGAATTCTTTTTTTGGTTGCTa                                >  2:3799/1‑150 (MQ=255)                                                                                                                   aaaaaTGGGGCGGATAGTGAGGCGGTTTTTGCTGCGCCTATTGAAAATTTTGAATTGAGCAAAACTTTTGGGAGATTAAGCCCAAAAAACCGTTTGGCAATGGTTTAGTTTCGCGCAGAGGGATGGGCTTAATTCTTTTTTTTGGTGCt                                 >  1:762128/1‑149 (MQ=255)                                                                                                                   aaaaaTGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTa                                <  1:730118/150‑1 (MQ=255)                                                                                                                     aaaTGGGGCGGATAGTGAGGCGGTTTTTGATGAGCTTATTTAACATTTATAATTGATAAATCCTTCTGGCCGATTTAGCCAAAATAACCGTTCGGCAAAGCTTCAGTTTCGCGAAGAGGAATGGGCTGAATTCTTTTTTTTGTTGCTa                                >  2:627545/1‑148 (MQ=255)                                                                                                                       aTGGGGCGGATAGTGAGGCGGTTTTGGGGGCGCCTATTGTACAATTAGAAATTATCAAATCTTCTGGGCGATTAAAGCAAAATAACCCCTCCGCAATGGTTCAGTTAACCCGAGAGGAAAGGGCTGAATTCttttttttggttccaaa                              >  2:427238/1‑138 (MQ=255)                                                                                                                              ggATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAg                    <  2:139236/151‑1 (MQ=255)                                                                                                                                                ggCTGAGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGatt  >  2:632819/1‑151 (MQ=255)                                                                                                                                                |                                                                                                                                                       ATCCGATAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATT  >  NC_000913/1245091‑1245384

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑