Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,861,866	0	G	T	48.1%	34.6 / 14.8	27	Q37K (CAA→AAA)	yeaC	DUF1315 family protein
Reads supporting (aligned to +/- strand):  ref base G (3/11);  new base T (13/0);  total (16/11)
Fisher's exact test for biased strand distribution p-value = 3.39e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAATAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGTGCTTCGATATTATGGCGGGCTTGCCACAGCATCACCAGTTGCAGGCAGTTCTCTTTTTGTTCCTCGGTCAACGCAACGCCATCAGGCCATTTCCCCAGTTCAACGGCGGTCGACAAACGCTGGTATACCTCAGGCATCATGCTGTTGATAATGTCATCAAGATTCATAATTTTTCCGCTCCTGTGGAATAAGTTGCTGAATC  >  NC_000913/1861716‑1862009                                                                                                                                                       |                                                                                                                                                cAATAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGTGCTTCGATATTATGGCGGGCTTGCCACAGCATCACCAGTTGCAGGCAGTTCTCTTTTTg                                                                                                                                                 <  2:369097/151‑1 (MQ=255)    tAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGTGCTTCGATATTATGGCGGGCTTGCCACAGCATCACCAGTTGCAGGCAGTTCTCTTTTTGtt                                                                                                                                               <  2:128659/150‑1 (MQ=255)           tGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGTGCTTCGATATTATGGCGGGCTTGCCACAGCATCACCAGTTGCAGGCAGTTCTCTTTTTGTTCCTCGGt                                                                                                                                        <  2:22292/150‑1 (MQ=255)            gCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGTGCTTCGATATTATGGCGGGGTTGGCCCAACATCACCCATTTCAGGCCGGTTTCTTTTTTTTCCTCGGt                                                                                                                                        >  2:516465/1‑149 (MQ=255)            gCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGTGCTTCGATATTATGGCGGGCTTGCCACAGCATCACCAGTTGCAGGCAGTTCTCTTTTTGTTCCTCGGTc                                                                                                                                       <  2:260327/150‑1 (MQ=255)              tGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGTGCTTCGATATTATGGCGGGCTTGGCACAGCATCACCAGCTGCAGGCCGGTCTCTTTTTTTTCCTCGGGcaa                                                                                                                                     >  2:809748/1‑150 (MQ=255)                     ccAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGTGCTTCGATATTATGGCGGGCTTTCCACAACATCACCAGATGCAGGCAGTTTTCTTTTTTTTTCTCGGTcaacgc                                                                                                                                  >  2:754276/1‑146 (MQ=255)                      cAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGTGCTTCGATATTATGGCGGGCTTGCCACAGCATCACCAGTTGCAGGCAGGTCTCTTTTTTTTTCTCGGTCAACCCAACGcc                                                                                                                            >  2:400664/1‑151 (MQ=255)                           tcttctTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGTGCTTCGATATTATGGCGGGCTTTCCACAACCTCACCACTTTCTGGCCGGTTTTTTTTTTTTTCTCGGGCCACGCAACCCCATc                                                                                                                         >  1:528272/1‑149 (MQ=255)                           tcttctTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGTGCTTCGATATTATGGCGGGCTTGCCACAGCATCACCAGTTGCAGGCAGTTCTCTTTTTTTTTCTCGGGcaacgcaa                                                                                                                                >  2:692267/1‑142 (MQ=255)                            cttcttTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGTGCTTCGATATTATGGCGGGCTTTCCCCAGCATCACCAGTTGCAGGCAGTTTTTTTTTTTTTTCTCGGTCAACGCAACGCCCTc                                                                                                                         >  1:215435/1‑148 (MQ=255)                                 tttAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGTGCTTCGATATTATGGCGGGCTTTCCACAACATCACCAGTTTCAGGCAGTTTTTTTTTTTTTTCTTGGGTAACGCCACCCCACCAAGCCa                                                                                                                   >  1:455220/1‑149 (MQ=255)                                      ctgctgTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGTGCTTCGATATTATGGCGGGCTTGCCACCGCATCACCAGTTGCAGGCAGGTCTCTTTTTTTTTCTCCGGCAACGCAACGCCCTCAGGCCATTTcccc                                                                                                            >  2:435638/1‑151 (MQ=255)                                          tgTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGTGCTTCGATATTATGGCGGGCTTGGCACAGCATCACCAGTTGCAGGGAGTTCTCTTTTTTTTCCTCCGTCAAAGCCACGCCCTCAAGCCCTTTTCCCCGtt                                                                                                        >  2:462502/1‑151 (MQ=255)                                             ttGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGTGCTTCGATATTATGGCGGGCTTGGCACAGCATCAACAGTTTCAGGGCGTTTTCTTTTTTTTTCTCGGTCAACGCCACGCCATCAGGGCATTTTCCCCGTTCa                                                                                                      >  2:662356/1‑150 (MQ=255)                                                     cATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGTGCTTCGATATTATGGCGGGCTTGCCACAGCATCACCAGTTGCAGGCAGTTCTCTTTTTGTTCCTCGGTCAACGCAACGCCATCAGGCCATTTCCCCAGTTCAACGGCGGTc                                                                                             <  2:455272/151‑1 (MQ=255)                                                                          gtCAATTGTCATATGCTGTGCTTCGATATTATGGCGGGCTTGCCACAGCATCACCAGTTGCAGGCAGTTCTCTTTTTGTTCCTCGGTCAACGCAACGCCATCAGGCCATTTCCCCAGTTCAACGGCGGTCGACAAACGCTGGTATACCTc                                                                         <  2:102507/150‑1 (MQ=255)                                                                             aaTTGTCATATGCTGTGCTTCGATATTATGGCGGGCTTGCCACCACATCACCCGTTGCAGGCAGGTTTCTTTTTTTTTCTCCGTCAACGCAACGCCCTCAGGCCATTTTCCCAGTTCAACGGCGGGCGACAAACACCGGGGTACCTccag                                                                      >  2:21851/1‑147 (MQ=255)                                                                                   cATATGCTGTGCTTCGATATTATGGCGGGCTTGCCACAGCATCACCAGTTGCAGGCAGTTCTCTTTTTGTTCCTCGGTCAACGCAACGCCATCAGGCCATTTCCCCAGTTCAACGGCGGTCGACAAACGCTGGTATACCTCAGGCATCATg                                                               <  2:215474/151‑1 (MQ=255)                                                                                    atatGCTGTGCTTCGATATTATGGCGGGCTTGCCCCAACATCCCCCGTTGCAGGCAGTTCTCTTTTTTTTTCTCGGTCAACGCAACACCATCCGGCCCCTTTCCCAGTTTCAAGGCGGGCGGCaaaaaccgg                                                                                 >  2:470365/1‑126 (MQ=255)                                                                                                tCGATATTATGGCGGGCTTGCCACAGCATCACCAGTTGCAGGCAGTTCTCTTTTTGTTCCTCGGTCAACGCAACGCCATCAGGCCATTTCCCCAGTTCAACGGCGGTCGACAAACGCTGGTATACCTCAGGCATCATGCTGTTGATAATg                                                   <  2:495408/150‑1 (MQ=255)                                                                                                tCGATATTATGGCGGGCTTGCCACAGCATCACCAGTTGCAGGCAGTTCTCTTTTTGTTCCTCGGTCAACGCAACGCCATCAGGCCATTTCCCCAGTTCAACGGCGGTCGACAAACGCTGGTATACCTCAGGCATCATGCTGTTGATAATg                                                   <  1:598378/150‑1 (MQ=255)                                                                                                   atatTATGGCGGGCTTGCCACAGCATCACCAGTTGCAGGCAGTTCTCTTTTTGTTCCTCGGTCAACGCAACGCCATCAGGCCATTTCCCCAGTTCAACGGCGGTCGACAAACGCTGGTATACCTCAGGCATCATGCTGTTGATAATGtcat                                               <  2:285183/151‑1 (MQ=255)                                                                                                               gCTTGCCACAGCATCACCAGTTGCAGGCAGTTCTCTTTTTGTTCCTCGGTCAACGCAACGCCATCAGGCCATTTCCCCAGTTCAACGGCGGTCGACAAACGCTGGTATACCTCAGGCATCATGCTGTTGATAATGTCATCAAGATTCATaa                                   <  2:330064/151‑1 (MQ=255)                                                                                                                cTTGCCACAGCATCACCAGTTGCAGGCAGTTCTCTTTTTGTTCCTCGGTCAACGCAACGCCATCAGGCCATTTCCCCAGTTCAACGGCGGTCGACAAACGCTGGTATACCTCAGGCATCATGCTGTTGATAATGTCATCAAGATTCATAAt                                  >  2:751691/1‑151 (MQ=255)                                                                                                                       caGCATCACCAGTTGCAGGCAGTTCTCTTTTTGTTCCTCGGTCAACGCAACGCCATCAGGCCATTTCCCCAGTTCAACGGCGGTCGACAAACGCCGGTATACCTCAGGCATCATGCTGTTGATAATGTCATCAAGATTCATAATTTTTCCg                           >  2:487881/1‑151 (MQ=255)                                                                                                                              aCCAGTTGCAGGCAGTTCTCTTTTTGTTCCTCGGTCAACGCAACGCCATCAGGCCATTTCCCCAGTTCAACGGCGGACGACAAACGGTGGGATACCTCAGGGATCATGCTGTTGATAATGTCATCAAGATTCATAATTTTTCCGCTCCtgt                    >  2:745823/1‑151 (MQ=255)                                                                                                                                                   ttttGTTCCTCGGTCAACGCAACGCCATCAGGCCATTTCCCCAGTTCAACGGCGGTCGACAAACGCTGGTATACCTCAGGCATCATGCTGTTGATAATGTCATCAAGATTCATAATTTTTCCGCTCCTGTGGAATAAGTTGCTGAATc  <  2:376086/148‑1 (MQ=255)                                                                                                                                                       |                                                                                                                                                CAATAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGTGCTTCGATATTATGGCGGGCTTGCCACAGCATCACCAGTTGCAGGCAGTTCTCTTTTTGTTCCTCGGTCAACGCAACGCCATCAGGCCATTTCCCCAGTTCAACGGCGGTCGACAAACGCTGGTATACCTCAGGCATCATGCTGTTGATAATGTCATCAAGATTCATAATTTTTCCGCTCCTGTGGAATAAGTTGCTGAATC  >  NC_000913/1861716‑1862009

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑