Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,434,882	0	T	G	37.6%	165.6 / 13.7	86	Y252S (TAC→TCC)	truA	tRNA pseudouridine(38‑40) synthase
Reads supporting (aligned to +/- strand):  ref base T (9/44);  new base G (32/0);  total (42/44)
Fisher's exact test for biased strand distribution p-value = 1.40e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGC‑GTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATAT  >  NC_000913/2434736‑2435032                                                                                                                                                   |                                                                                                                                                         gTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGTAGATCATACCGGCCGGGGGaa                                                                                                                                                        >  1:116012/1‑149 (MQ=255)   ccATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATACCCGGCAGGGTAAAcc                                                                                                                                                     >  1:675513/1‑150 (MQ=255)    cATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATACCGGGCAGGGGAATCCAc                                                                                                                                                   >  2:83368/1‑151 (MQ=255)      tatTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGGAAaccaccc                                                                                                                                                 >  1:85987/1‑150 (MQ=255)       atTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACcg                                                                                                                                                 <  2:557359/150‑1 (MQ=255)          tGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCCGGCAGGGGAAACCACcgc                                                                                                                                                >  2:368217/1‑148 (MQ=255)          tGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATAACGGTCAGGGGAATCCAcc                                                                                                                                                  >  2:712972/1‑146 (MQ=255)           gCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGAACATACCGGTCAGGGGAAACCACCGCGGcc                                                                                                                                            >  2:83369/1‑151 (MQ=255)           gCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGAcc                                                                                                                                            <  1:788563/151‑1 (MQ=255)           gCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGAc                                                                                                                                             <  2:213078/150‑1 (MQ=255)            ccTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGGTTTTGAAGAAGATTCCGGGCAGGGGAATCCAcc                                                                                                                                                  >  2:600873/1‑144 (MQ=255)               aTGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAg                                                                                                                                          <  1:283167/149‑1 (MQ=255)               aTGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGa                                                                                                                                         <  2:219289/150‑1 (MQ=255)                tGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCGGGCAGGGGAATCCACCCCGAcc                                                                                                                                            >  2:238157/1‑146 (MQ=255)                        aCTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCtt                                                                                                                              <  2:788877/151‑1 (MQ=255)                        aCTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCtt                                                                                                                              <  2:691727/151‑1 (MQ=255)                        aCTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGATTTGGAGGATGATAACTGCCAGGGGAATCCCCCGCGCCCCGATACAG‑CCCtt                                                                                                                              >  1:747532/1‑151 (MQ=255)                              cATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATACCCGGCAGGGGAATCCCCCGCGACCCGAAACAG‑CCCTTcccc                                                                                                                          >  1:102152/1‑147 (MQ=255)                               aTCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCCTACCCGTCAGGGGAAACCACCCCGGCCAGATAAAGCcccttcccctt                                                                                                                        >  2:286795/1‑151 (MQ=255)                               aTCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCGGTCAGGGGAATCCACCGCGACCCGATACAG‑CCCTTCCGCttt                                                                                                                       >  1:33128/1‑151 (MQ=255)                                   ttCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCtttt                                                                                                                      <  1:339985/148‑1 (MQ=255)                                     cAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGGCAGGGGAATCCACCGCGACCAGATACAG‑CCCTTCCGCCTTTTcc                                                                                                                   >  2:119564/1‑149 (MQ=255)                                             aaCTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGAGCATCCCGGGCCGGGGAAACCCCCGCGCCCAGGTACAG‑CCCCTCCCCCTTTTCCGGTGCTtcc                                                                                                          >  1:480314/1‑150 (MQ=255)                                             aaCTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGGAGATCATACCGGGCAGGGGAAACCACCCCGGCCCGATACAG‑ACCCTCCGCTTTTTCCGTTGCTgcc                                                                                                          >  1:517117/1‑150 (MQ=255)                                             aaCTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTgccg                                                                                                         <  2:807855/151‑1 (MQ=255)                                                tACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGGAGATCATACCGGTCAGGGGAATCCACCCCGACCAGATACAG‑CCCCTCCGCTTTTTCCGTTGCTgccccc                                                                                                       >  2:503885/1‑150 (MQ=255)                                                tACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGGCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTTCCGTTGCTgcccc                                                                                                        >  2:175697/1‑147 (MQ=255)                                                 aCGTTGGCTACAGCCTGATATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAg                                                                                                     <  1:144066/151‑1 (MQ=255)                                                 aCGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTgccgcc                                                                                                       <  2:748590/149‑1 (MQ=255)                                                 aCGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTgccgcc                                                                                                       <  2:132992/149‑1 (MQ=255)                                                  cGTTGGCTACAGACTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGTTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGc                                                                                                    <  1:277777/151‑1 (MQ=255)                                                    ttGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGATTTTGAAGATCATACCGGTCAGGGGAATCCCCCGCGACCAGATACAA‑CCCTTCCCCTTTTGCCGGTGCTGCCGCCAGC‑g                                                                                                  >  2:394462/1‑150 (MQ=255)                                                       gCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGC‑GTTCt                                                                                              <  2:761774/151‑1 (MQ=255)                                                           cAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGC‑GTTCTGTcc                                                                                          <  2:783098/151‑1 (MQ=255)                                                              catGAAATGTTCCGGGCATTAATCTCGTGAGTCCGCCCGAAATAACGGGCCGATTGGCGGTTTTGGAAGATCTTACCGGTCAGGGTAATCCACCGCGACCGGATCCGG‑CCCTGCCGCTTTTGCCGTTGCTGCCGCCAGC‑GTTCTGTCCttt                                                                                       <  1:505285/149‑1 (MQ=255)                                                               cTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATACCGGGCAGGGGAATCCACCGCGGCCCGAAACAG‑CCCTTCCGCTTTTTCCGTTGCTGCCCCCCGC‑GTTCTGTTCttt                                                                                       >  1:125345/1‑150 (MQ=255)                                                                 gAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCGGTCAGGGGAAACCACCGCGACCAGATACAA‑CCCTTCCGCTTTTTCCGGTGCTGCCCCCCGC‑GTTCTGTCCTTTgcc                                                                                    >  2:431221/1‑151 (MQ=255)                                                                 gAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGC‑GTTCTGTCCTTTg                                                                                      <  2:585056/149‑1 (MQ=255)                                                                  aaaTGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGGCAGGGGAATCCACCGCGACCAGATACAG‑CCCCTCCGCTTTTTCCGTTGCTGCCGCCCGC‑GTTCTGTCCTTTgcc                                                                                    >  2:196588/1‑150 (MQ=255)                                                                      gTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGC‑GTTCTGTCCTTTGCCGCCa                                                                                <  1:770098/150‑1 (MQ=255)                                                                       ttCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGGAGATCATACCGGGCAGGGGAATCCACCGCGACCAGATACAC‑CCCTTCCGCTTTTTCCGTTGCTGCCGCCCGC‑GCTCTGTCCTTTTccgcccgc                                                                              >  2:470535/1‑151 (MQ=255)                                                                         ccGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGC‑GTTCTGTCCTTTGCCGCCAGCa                                                                             <  2:444202/150‑1 (MQ=255)                                                                         ccGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGC‑GTTCTGTCCTTTGCCGCCAGCa                                                                             <  1:579408/150‑1 (MQ=255)                                                                          cGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATACCGGGCAGGGGAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTTCCGTTGCTGCCCCCCGC‑GGTCTGTCCTTTTCCCCCCGcca                                                                            >  2:703367/1‑148 (MQ=255)                                                                          cGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGC‑GTTCTGTCCTTTGCCGCCAGCAAc                                                                           <  1:321178/151‑1 (MQ=255)                                                                             gCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGATATTTTACCCGGCATAGGAAACCACCGCCACCAGATAAAA‑CCCTTCCCCTTTTTCCACTGCTGCACCCAGCGGTTCGATCCTTTGCC‑CCACCAActc                                                                         >  1:492948/1‑150 (MQ=255)                                                                               attattCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCGGTCAGGGGAATCCACCGCGACCCGATACAA‑CCCTTCCGCTTTTTCCGGTGCCGCCCCCAAA‑GTTTTCTCCCTTTGCGCCCGCAAccc                                                                         >  2:639926/1‑146 (MQ=255)                                                                               attattCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCGGGCAGGGGAATCCACCCCGACCAGATAAAG‑CCCCTCCGCCTTTTCCGTTGCTGCCCCCAGC‑GTTCTGTCCTTTGccgcccgcca                                                                            >  1:152452/1‑143 (MQ=255)                                                                                 tattCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGC‑GTTCTGTCCTTTGCCGCCAGCAACTCTGCt                                                                     <  2:152490/150‑1 (MQ=255)                                                                                  attCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGC‑GTTCTGTCCTTTGCCGCCAGCAACTCTGCTa                                                                    <  1:304798/150‑1 (MQ=255)                                                                                     ctcGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGGAAACCACCCCGACCAGATACAG‑CCCTTCCCCCTTTTCCGGTGCTGCCGCCCGC‑GGTTTGTCCTTTGCCCCCCGCAACTCTGCTATcc                                                                 >  2:496129/1‑150 (MQ=255)                                                                                       cGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGC‑GTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGc                                                              <  2:715138/151‑1 (MQ=255)                                                                                        gTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCGGCCCGGGGAAACCACCGCGACCAGATACAG‑ACCTTCCGCCTTTGCCGTTGCTGCCGCCAGC‑GTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGc                                                              >  1:118947/1‑150 (MQ=255)                                                                                         ttAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTGTTGCAGATCATACCCGGGAGGGGAATCCACCGCGACCAGAAACCG‑GCCCTACGCTTTTGGCTGTTCTGCCCCCCGG‑GCTATGTCCTTTGCCCCCCCCCACTCTTCTATCCCGctc                                                            >  2:761146/1‑151 (MQ=255)                                                                                           aGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGC‑GTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCtctc                                                          <  2:167369/151‑1 (MQ=255)                                                                                           aGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGGCAGGGGAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGC‑GTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGctct                                                           >  2:289938/1‑150 (MQ=255)                                                                                            gTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGC‑GTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTcc                                                         <  2:83494/151‑1 (MQ=255)                                                                                             tCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGGCAGGGGAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTTCCGTTGCTGCCGCCAGC‑GTTCTGTCCTTTTCCGCCCGCAACTCTGCTATCCCGCTCTcc                                                         >  2:621132/1‑150 (MQ=255)                                                                                               cGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCGGTCAGGGGAATCCACCCCGACCAGATACAG‑CCCTTCCGCTTTTTCCGTTGCTTCCCCCCGC‑GGTTTGTCCTTTGCCCCCAGCAACTCTGCTATCCCGCTCTCCgg                                                       >  1:45695/1‑150 (MQ=255)                                                                                                 ccAGAAATAGCGGGCCCATTGGCGGTGTTGGAAGCCCATACCGGTCAGGGTAATCCCCTGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCCCCAGC‑GTCCTGTCCTTTGCCGCCAGCAACGCTCCTATCCAGCTATCCGGc                                                      <  1:781039/149‑1 (MQ=255)                                                                                                    gAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGC‑GTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGtt                                                 <  2:525863/151‑1 (MQ=255)                                                                                                      aaTAGCGGGCCCATTGGCGGTTTTTGAAGATCATAACCGGCAGGGGAATCCCCCGCGACCCGATACCG‑CCCCTCCCCTTTTTCCGTTGCTGCCCCCCGC‑GGTCTGTCCTTTTCCCCCCGCAAATCTGCTATCCAGCTCTCCCGCTGGtt                                                 >  1:161270/1‑149 (MQ=255)                                                                                                                 cATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGC‑GTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACt                                    <  2:541775/151‑1 (MQ=255)                                                                                                                      tcGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGC‑GTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCAt                               >  1:94692/2‑151 (MQ=255)                                                                                                                       cGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGC‑GTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATc                              >  2:488858/1‑151 (MQ=255)                                                                                                                          ttttGGAAGATCATACCGGTCAGGGAAATCCACCGCGACCAGATTCAG‑CCCTTCCGCTTTTGCCCTTGCTGCCCCCAGC‑GCTCTGTCCTTTCCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAgg                           >  1:45612/1‑151 (MQ=255)                                                                                                                             tGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGC‑GTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCt                         <  2:45733/150‑1 (MQ=255)                                                                                                                             tGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGC‑GTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTg                        >  1:136717/1‑151 (MQ=255)                                                                                                                                 aGATCCTCCCGGTCAGGGTAACCCCCCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTCCCGCCAGT‑GTTCTGTCCTTTGCCGCCATCAACTCTGCTCTCCAGCTCTCCGGTTGGTTGTGGGCACCGACTTCCATCAGGCTg                        <  1:209598/147‑1 (MQ=255)                                                                                                                                 aGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGC‑GTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGa                    <  2:747617/151‑1 (MQ=255)                                                                                                                                      aTACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGC‑GTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAata               <  1:248351/151‑1 (MQ=255)                                                                                                                                       tACCGGTTAGTGTATTCCGCCGCGACCAGCTACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGC‑GTTCTGTGCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGGTGTGGGCACCGCCTTCCATCAGGCTGCCGACAat                <  1:429924/149‑1 (MQ=255)                                                                                                                                        aCCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGC‑GTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATAtt             <  2:511806/151‑1 (MQ=255)                                                                                                                                        aCCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGC‑GTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATAtt             <  2:177993/151‑1 (MQ=255)                                                                                                                                         ccGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGC‑GTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTc            <  1:616217/151‑1 (MQ=255)                                                                                                                                           ggTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGC‑GTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCt          >  2:551147/1‑151 (MQ=255)                                                                                                                                            gTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGC‑GTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTg         <  1:386488/151‑1 (MQ=255)                                                                                                                                            gTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGC‑GTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTg         <  1:156168/151‑1 (MQ=255)                                                                                                                                            gTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGC‑GTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTg         >  2:53377/1‑151 (MQ=255)                                                                                                                                             tCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGC‑GTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTg         <  2:211597/150‑1 (MQ=255)                                                                                                                                              cAGGGTAATCGACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGC‑GTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCAGCGACTTCCATCAGGCTGCCGACAATATTCCTGAc       <  1:494909/151‑1 (MQ=255)                                                                                                                                              cAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGC‑GTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGAc       <  2:808941/151‑1 (MQ=255)                                                                                                                                               aGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGC‑GTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGAcc      <  2:214845/151‑1 (MQ=255)                                                                                                                                               aGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGC‑GTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGAcc      >  2:128959/1‑151 (MQ=255)                                                                                                                                               aGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGC‑GTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGAcc      <  2:116049/151‑1 (MQ=255)                                                                                                                                                  gTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGC‑GTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCat    >  1:86093/1‑150 (MQ=255)                                                                                                                                                   tAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGC‑GTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCatat  >  1:320306/1‑151 (MQ=255)                                                                                                                                                   |                                                                                                                                                         GTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGC‑GTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATAT  >  NC_000913/2434736‑2435032

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑