BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,913,536	C→T	W39* (TGG→TAG)	relA ←	(p)ppGpp synthetase I/GTP pyrophosphokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,913,536	0	C	T	100.0%	402.3 / NA	104	W39* (TGG→TAG)	relA	(p)ppGpp synthetase I/GTP pyrophosphokinase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (53/51); total (53/51)

Only 100 of 104 total aligned reads displayed.

CATCCGCCAGAGGGAAAAGCAGCGCCGCCCGCAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCTCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCCAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCT  >  NC_000913/2913393‑2913677

cATCCGCCAGAGGGAAAAGCAGCGCCGCCCGCAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCTCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCTAGGTTTc                                                                                                                                        >  1:109614/1‑151 (MQ=255)

 aTCCGCCAGAGGGAAAAGCAGCGCCGCCCGCAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCTCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCTAGGTTTCg                                                                                                                                       >  2:533005/1‑151 (MQ=255)

 aTCCGCCAGAGGGAAAAGCAGCGCCGCCCGCAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCTCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCTAGGTTTCg                                                                                                                                       <  2:411917/151‑1 (MQ=255)

   ccGCCAGAGGGAAAAGCAGCGCCGCCCGCAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCTCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCTAGGTTTCGGc                                                                                                                                     >  1:231467/1‑151 (MQ=255)

    cGCCAGAGGGAAAAGCAGCGCCGCCCGCAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCTCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCTAGGTTTCGGCt                                                                                                                                    <  2:671612/151‑1 (MQ=255)

    cGCCAGAGGGAAAAGCAGCGCCGCCCGCAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCTCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCTAGGTTTCGGCt                                                                                                                                    >  2:209272/1‑151 (MQ=255)

       cAGAGGGAAAAGCAGCGCCGCCCGCAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCTCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCTAGGTTTCGGCTa                                                                                                                                   >  1:705664/1‑149 (MQ=255)

       cAGAGGGAAAAGCAGCGCCGCCCGCAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCTCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCTAGGTTTCGGCTAAg                                                                                                                                 <  1:749836/151‑1 (MQ=255)

        agagTGAAAAGCCGCGCCGCCCGCAGCGGGTCAATGTCCATACTTAATGTCGAGAGGATCTCCACCATCTCATCCCCACGCCACAATAACAAACTGGCACCCGGATGCCCCTGCGTCTGTGGCAGACAATACGCCTAGGTTTCGGCGAAGc                                                                                                                                <  1:772726/151‑1 (MQ=255)

             gAAAAGCAGCGCCGCCCGCAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCTCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCTAGGTTTCGGCTAAGCACTc                                                                                                                            >  2:511404/1‑150 (MQ=255)

                aaGCAGCGCCGCCCGCAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCTCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCTAGGTTTCGGCTAAGCACTCacac                                                                                                                        >  1:267510/1‑151 (MQ=255)

                 agcagcGCCGCCCGCAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCTCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCTAGGTTTCGGCTAAGCACTCACACg                                                                                                                       >  2:328586/1‑151 (MQ=255)

                  gcagcGCCGCCCGCAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCTCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCTAGGTTTCGGCTAAGCACTCACACg                                                                                                                       <  1:94650/150‑1 (MQ=255)

                    agcGCCGCCCGCAGCGGGTCAATGTCCATACTTAATGTCGAGAGGATCTCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCTAGGTTTCGGCTAAGCACTCACACGACt                                                                                                                    >  1:25498/1‑151 (MQ=255)

                     gcgcCGCCCGCAGCGTGTCAATGTCCATACTTAATGTCGAGAGGAGCTCCACCATCGCAACACCACGCCACAATAGCAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCTAGGTTTCGGCTAAGCACTCACACGACtt                                                                                                                   <  1:126963/151‑1 (MQ=255)

                      cgccgcCCGCAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCTCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCGGACAATACGCCTAGGTTTCGGCTAAGCACTCACACGACtt                                                                                                                   >  1:734138/1‑150 (MQ=255)

                        ccgcccgcAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCTCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCTAGGTTTCGGCTAAGCACTCACACGACTTctg                                                                                                                >  1:791006/1‑151 (MQ=255)

                           cccgcAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCTCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCTAGGTTTCGGCTAAGCACTCACACGACTTctggct                                                                                                             <  1:622288/151‑1 (MQ=255)

                             cgcAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCTCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCTAGGTTTCGGCTAAGCACTCACACGACTTctggctgg                                                                                                           <  1:772144/151‑1 (MQ=255)

                             cgcAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCTCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCTAGGTTTCGGCTAAGCACTCACACGACTTctggctgg                                                                                                           <  1:474284/151‑1 (MQ=255)

                               cAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCTCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCTAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGt                                                                                                          >  2:221518/1‑150 (MQ=255)

                               cAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCTCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCTAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTa                                                                                                         <  1:327529/151‑1 (MQ=255)

                                aGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCTCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCTAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTa                                                                                                         >  1:256491/1‑150 (MQ=255)

                                aGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCTCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCTAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTa                                                                                                         >  2:748631/1‑150 (MQ=255)

                                       cAATGTCCATACTTAATGTCGGGAGGTTCTCCACCACCTCAACACCACGCCTCAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCTAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAg                                                                                                 <  1:411295/151‑1 (MQ=255)

                                       cAATGTCCATACTTAATGTCGAGAGGATCTCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCTAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAg                                                                                                 <  2:24958/151‑1 (MQ=255)

                                        aaTGTCCATACTTAATGTCGAGAGGATCTCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCTAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATAccc                                                                                                   >  1:484733/1‑148 (MQ=255)

                                           gTCCATACTTAATGTCGAGAGGATCTCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCTAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAGACt                                                                                              <  1:359699/150‑1 (MQ=255)

                                           gTCCATACTTAATGTCGAGAGGATCTCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCTAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAGACt                                                                                              <  2:513352/150‑1 (MQ=255)

                                           gTCCATACTTAATGTCGAGAGGATCTCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCTAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAGACt                                                                                              <  2:798141/150‑1 (MQ=255)

                                            tCCATACTTAATGTCGAGAGGATCTCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCTAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAGACTTg                                                                                            >  2:445615/1‑151 (MQ=255)

                                             ccATACTTAATGTCGAGAGGATCTCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCTAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAGACTTGc                                                                                           <  1:521159/151‑1 (MQ=255)

                                              cATACTTAATGTCGAGAGGATCTCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCTAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAGACtt                                                                                             <  1:757185/148‑1 (MQ=255)

                                                tACTTAATGTCGAGAGGATCTCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCTAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAGACTTGCGAt                                                                                        >  1:404428/1‑151 (MQ=255)

                                                 aCTTAATGTCGAGAGGATCTCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCTAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAGACTTGCGAt                                                                                        <  1:555290/150‑1 (MQ=255)

                                                   ttAATGTCGAGAGGATCTCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCTAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAGACTTGCGATcc                                                                                      >  1:663765/1‑150 (MQ=255)

                                                    tAATGTCGAGAGGATCTCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCTAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAGACTTGCGATCCAt                                                                                    >  2:625410/1‑151 (MQ=255)

                                                    tAATGTCGAGAGGATCTCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCTAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAGACTTGCGATCCAt                                                                                    >  1:207040/1‑151 (MQ=255)

                                                     aaTGTCGAGAGGATCTCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCTAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAGACTTGCGATCCAtt                                                                                   >  2:27304/1‑151 (MQ=255)

                                                      aTGTCGAGAGGATCTCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCTAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAGACTTGCGATCCAttt                                                                                  <  2:518292/151‑1 (MQ=255)

                                                       tGTCGAGAGCATCTCCACCATCTCAACACCACGCCACAATAACAGACTGTCATCCGGATGCCCCAGCGTCTGAAGCCGACAATACGCCTAGGTTTCGGCTAAGCAATCACACGACTACTGGCTGGTAATACCCAGACTTGCGATCGAtt                                                                                   >  1:169234/1‑149 (MQ=255)

                                                        gTCGAGAGGATCTCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCTAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAGACTTGCGATCCAtttt                                                                                 <  1:769668/150‑1 (MQ=255)

                                                         tCGAGAGGATCTCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCTAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAGACTTGCGATCCATTTTTc                                                                               >  2:450054/1‑151 (MQ=255)

                                                            agagGATCTCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCTAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCgg                                                                            >  1:614595/1‑151 (MQ=255)

                                                            agagGATCTCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCTAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCgg                                                                            >  1:597162/1‑151 (MQ=255)

                                                            agagGATCTCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCTAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCgg                                                                            >  1:440607/1‑151 (MQ=255)

                                                             gagGATCTCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCTAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCgg                                                                            >  2:39785/1‑150 (MQ=255)

                                                             gagGATCTCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCTAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCgg                                                                            >  2:2887/1‑150 (MQ=255)

                                                              agGATCTCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCTAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGa                                                                           <  2:361557/150‑1 (MQ=255)

                                                              agGATCTCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCTAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGAt                                                                          <  2:13476/151‑1 (MQ=255)

                                                               ggATCTCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCTAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATc                                                                         <  2:634303/151‑1 (MQ=255)

                                                               ggATCTCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCTAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATc                                                                         <  2:385353/151‑1 (MQ=255)

                                                                 aTCTCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCTAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATCa                                                                        >  1:759009/1‑150 (MQ=255)

                                                                  tctcCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCTAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATAccc                                                                                                   <  2:48964/122‑1 (MQ=255)

                                                                  tctcCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCTAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATAccc                                                                                                   >  1:48926/1‑122 (MQ=255)

                                                                  tctcCACAATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCTAGGTTTCGGCTAAGCACTCAAACGCCTTCTGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATCaa                                                                       >  1:527830/1‑150 (MQ=255)

                                                                   ctcCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCTAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATCAAAt                                                                     >  1:350654/1‑151 (MQ=255)

                                                                   ctcCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCTAGGTTTCGGCTAAGCACTCACACGACTTCTGGCCGGTAATACCCAGACTTGCGATCCATTTTTCCGGATCAAAt                                                                     >  2:741148/1‑151 (MQ=255)

                                                                     ccaccaTCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCTAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATCa                                                                        <  1:398682/146‑1 (MQ=255)

                                                                     ccaccaTCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCTAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATCa                                                                        <  2:347242/146‑1 (MQ=255)

                                                                       accaTCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCTAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATCAAATTCAc                                                                 >  1:209712/1‑151 (MQ=255)

                                                                            ctcAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCTAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATCAAATTCACCAGcc                                                            >  2:383269/1‑151 (MQ=255)

                                                                               aaCACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCTAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCtt                                                          >  1:67659/1‑150 (MQ=255)

                                                                               aaCACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCTAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCtt                                                          >  2:755279/1‑150 (MQ=255)

                                                                                 caccacGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCTAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTAtt                                                       >  1:463022/1‑151 (MQ=255)

                                                                                 caccacGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCTAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCAAGACTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTAt                                                        <  1:165602/150‑1 (MQ=255)

                                                                                   ccacGCCTCAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCTAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATCAAATTCCCCAGCCTTATTg                                                      <  2:496162/150‑1 (MQ=255)

                                                                                   ccacGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCTAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATCAAATTCACCAGc                                                             <  1:444648/143‑1 (MQ=255)

                                                                                    cacGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCTAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGat                                                    >  1:103279/1‑151 (MQ=255)

                                                                                     acGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCTAGGTTTCGGCTAAGCTCTCACACGACTTCTGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGata                                                   <  2:685580/151‑1 (MQ=255)

                                                                                       gCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCTAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATAtg                                                 >  2:102021/1‑151 (MQ=255)

                                                                                       gCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCTAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATAtg                                                 <  2:425207/151‑1 (MQ=255)

                                                                                       gCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCTAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATAtg                                                 <  2:305910/151‑1 (MQ=255)

                                                                                        ccACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCTAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATAtgt                                                <  2:772771/151‑1 (MQ=255)

                                                                                         cacaATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCTAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATAtgtg                                               >  1:58680/1‑151 (MQ=255)

                                                                                           caATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCTAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCa                                             <  1:511351/151‑1 (MQ=255)

                                                                                             aTAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCTAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCAct                                           <  2:150241/151‑1 (MQ=255)

                                                                                                 cAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCTAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCActtctt                                       <  2:809738/151‑1 (MQ=255)

                                                                                                  aGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCTAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTa                                      <  1:388055/151‑1 (MQ=255)

                                                                                                   gACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCTAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTAc                                     >  1:261050/1‑151 (MQ=255)

                                                                                                   gACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCTAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTAc                                     <  2:226673/151‑1 (MQ=255)

                                                                                                       ggCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCTAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGc                                  <  1:25517/150‑1 (MQ=255)

                                                                                                          aTCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCTAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAAcc                              >  2:216833/1‑151 (MQ=255)

                                                                                                              ggATGCCCCTGCGTCTGTTGCAGACAATACGCCTAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCg                          >  2:365921/1‑151 (MQ=255)

                                                                                                               gATGCCCCTGCGTCTGTTGCAGACAATACGCCTAGGTTTCGGCTAAGCACTCACACGACTTCTGTCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGt                         <  2:128173/151‑1 (MQ=255)

                                                                                                                   ccccTGCGTCTGTTGCAGACAATACGCCTAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCctc                     <  1:9501/151‑1 (MQ=255)

                                                                                                                    cccTGCGTCTGTTGCAGACAATACGCCTAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCctct                    <  2:180300/151‑1 (MQ=255)

                                                                                                                     ccTGCGTCTGTTGCAGACAATACGCCTAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCtctc                   >  1:698854/1‑151 (MQ=255)

                                                                                                                       tGCGTCTGTTGCAGACAATACGCCTAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCt                 >  2:92263/1‑151 (MQ=255)

                                                                                                                        gCGTCTGTTGCAGACAATACGCCTAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTcc                  <  2:657560/149‑1 (MQ=255)

                                                                                                                        gCGTCTGTTGCAGACAATACGCCTAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCtt                <  1:2887/151‑1 (MQ=255)

                                                                                                                        gCGTCTGTTGCAGACAATACGCCTAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCt                 <  1:672444/150‑1 (MQ=255)

                                                                                                                         cGTCTGTTGCAGACAATACGCCTAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCttt               >  2:85846/1‑151 (MQ=255)

                                                                                                                            cTGTTGCAGACAATACGCCTAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAgg            >  2:723760/1‑151 (MQ=255)

                                                                                                                             tGTTGCAGACAATACGCCTAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAggg           >  2:222320/1‑151 (MQ=255)

                                                                                                                              gTTGCAGACAATACGCCTAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGa          <  2:555757/151‑1 (MQ=255)

                                                                                                                                tGCAGACAATACGCCTAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGAcc        >  2:80030/1‑151 (MQ=255)

                                                                                                                                 gCAGACAATACGCCTAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCa       <  1:516336/151‑1 (MQ=255)

                                                                                                                                     aCAATACGCCTAGGTTTCGACTAAGCACTCACACGACTCCTGGCTGGTAATACCGAGACTTGCGATCCATTTGTCCGGATCAAAGTCACCAGCCTTATTGAGATGTGCGCTTCTTACCGCAACCATCGTCCCCTAATTGAGGGACCAGAcc   <  1:590100/151‑1 (MQ=255)

                                                                                                                                      cAATACGCCTAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCt  >  2:734154/1‑151 (MQ=255)

CATCCGCCAGAGGGAAAAGCAGCGCCGCCCGCAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCTCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCCAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCT  >  NC_000913/2913393‑2913677

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`