Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,206,435	0	C	T	47.7%	46.2 / 53.4	65	intergenic (‑179/‑28)	ttdR/ttdA	transcriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (3/31);  new base T (31/0);  total (34/31)
Fisher's exact test for biased strand distribution p-value = 2.07e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAA‑TACAAAAACTGGAGTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTC‑GCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAA  >  NC_000913/3206287‑3206583                                                                                                                                                      |                                                                                                                                                        gtgtATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCa                                                                                                                                                       <  2:715465/151‑1 (MQ=255)     aTTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GGATTTTTTCAGa                                                                                                                                                     >  2:159153/1‑149 (MQ=255)     aTTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACa                                                                                                                                                   <  2:236787/151‑1 (MQ=255)      tttCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAAACa                                                                                                                                                   >  1:287916/1‑150 (MQ=255)          gCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAAATTTTTcaaacaaaaa                                                                                                                                               >  2:405123/1‑148 (MQ=255)           cAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGAATTTTTccgaaaa                                                                                                                                                   >  1:654428/1‑141 (MQ=255)           cAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑TGATTTTTTCaagcca                                                                                                                                                  >  2:65276/1‑141 (MQ=255)           cAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCTGAaaaaaaa                                                                                                                                              >  1:697784/1‑147 (MQ=255)            aaaaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCCGACAAA‑AACa                                                                                                                                            >  2:459128/1‑150 (MQ=255)            aaaaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGG‑GGAATTTTTCAGACaaaaaaa                                                                                                                                             >  1:62402/1‑146 (MQ=255)             aaaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAA‑TACaaa                                                                                                                                          <  1:97870/151‑1 (MQ=255)             aaaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAA‑TACaaa                                                                                                                                          <  1:589078/151‑1 (MQ=255)              aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAA‑TACaaaa                                                                                                                                         <  2:435544/151‑1 (MQ=255)                cATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAA‑TACAAAAAc                                                                                                                                       <  2:641749/151‑1 (MQ=255)                       aTTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAGACAAA‑AAAAAAAAATGGGGt                                                                                                                                 >  1:381854/1‑150 (MQ=255)                       aTTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAAAAAAA‑AAAAAAAAATGGAg                                                                                                                                  >  1:767703/1‑149 (MQ=255)                         tCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTTAGACAAA‑AAAAAAAAATGGAg                                                                                                                                  >  1:826612/1‑147 (MQ=255)                           ccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAA‑TACAAAAACTGGAGTTGCCa                                                                                                                            <  1:279446/151‑1 (MQ=255)                              gCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAA‑TACAAAAACTGGAGTTGCCatg                                                                                                                          <  2:134135/150‑1 (MQ=255)                                   ggCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAA‑TACAAAAACTGGAGTTGCCATGATGA‑g                                                                                                                    <  1:22966/150‑1 (MQ=255)                                      gcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAA‑TACAAAAACTGGAGTTGCCATGATGA‑GCGaa                                                                                                                <  2:115888/151‑1 (MQ=255)                                        aaTATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAAACAAA‑AAAAAAAAATGGAGGTGCCATGATGA‑GCGaa                                                                                                                >  2:15629/1‑149 (MQ=255)                                        aaTATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAA‑TACAAAAACTGGAGTTGCCATGATGA‑GCGAAAg                                                                                                              <  2:395743/151‑1 (MQ=255)                                              cattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAA‑TACAAAAACTGGAGTTGCCATGATGA‑GCGAAAGtaataa                                                                                                        <  1:389419/151‑1 (MQ=255)                                               attcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAAACAAA‑AAAAAAAAATTGAGTTTGCATGGTGA‑GCGGAAGtaaaaa                                                                                                        >  1:256220/1‑150 (MQ=255)                                                 tcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAA‑TACAAAAACTGGAGTTGCCATGATGA‑GCGAAAGTAATAAGc                                                                                                      <  2:101230/150‑1 (MQ=255)                                                  cTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAA‑TAAAAAAACTGGAGTTGCCATGATGA‑GCGAAAGTAATAAGCa                                                                                                     >  1:450203/1‑150 (MQ=255)                                                    tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAGACAAA‑AAAAAAAAATGGAGTTGGCCAGATGA‑GGGGAAAgaataaaaaa                                                                                                    >  2:471032/1‑145 (MQ=255)                                                    tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAA‑TACAAAAACTGGAGTTGCCATGATGA‑GCGAAAGTAATAAGCa                                                                                                     <  1:499917/148‑1 (MQ=255)                                                    tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAA‑TACAAAAACTGGAGTTGCCATGATGA‑GCGAAAGTAATAAGCa                                                                                                     <  1:552615/148‑1 (MQ=255)                                                    tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAA‑TACAAAAACTGGAGTTGCCATGATGA‑GCGAAAGTAATAAGCAAc                                                                                                   <  1:239231/150‑1 (MQ=255)                                                        ttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAA‑TACAAAAACTGGAGTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGc                                                                                               <  1:405094/150‑1 (MQ=255)                                                        ttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAAATTTTTCAAAAAAA‑AACAAAAAATGGAGGTTGCATGATGA‑GGGGAAGaaaaaaaaaaaa                                                                                                  >  1:569016/1‑141 (MQ=255)                                                         tCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GCATTTTTTCAAACAAA‑TACAAAAAAATGGGTTTCCCTTATGG‑GTTAAAGGAATAAACAAAAgg                                                                                                >  1:729033/1‑148 (MQ=255)                                                         tCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAA‑TACAAAAACTGGAGTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCa                                                                                              <  2:493783/150‑1 (MQ=255)                                                             gTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAA‑TACAAAAACTGGAGTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAGTGaa                                                                                         <  1:246309/151‑1 (MQ=255)                                                               tGATTCACGAACTGTTAATTACATATTCACTGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAA‑TACAAAAACTGGAGTTGCCATGATTA‑GCGAAAGTAATAAGCAACAGGCAGt                                                                                            <  2:105839/146‑1 (MQ=255)                                                               tGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GGATTTTTTCAAACAAA‑AACAAAAAATGGAGTTGCCCTGATGA‑GCGAAAGAAATAAGCAAAAGGCAgggga                                                                                         >  2:38536/1‑145 (MQ=255)                                                               tGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGG‑GAAATTTTTCAGACAAA‑AAAAAAAAATGGGGGTGGCCTGGTGA‑GCGAGAGAAATAAAAAACAAGGAGt                                                                                            >  1:105826/1‑146 (MQ=255)                                                                 aTTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCATACAAA‑TACAAAAAATGGAGTTTCCCTGATGT‑GTGGAAATAATAAAAAACAAGCAGTGAATaa                                                                                      >  1:348809/1‑150 (MQ=255)                                                                            gTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAAACAAA‑AAAAAAAAATGGAGTTTGCATGATGA‑GCGAAAGTAATAAAAAAAAGGCAGTGAATAAATTGACag                                                                              >  1:83471/1‑147 (MQ=255)                                                                               aaTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAAATTTTTCAAACAAA‑AACAAAAAATGGGGGTTTCCTGATGG‑GGGAAAATAAAAAAAAAAAAGCAGTGAATAAAATGACAGAGAtt                                                                         >  1:828046/1‑149 (MQ=255)                                                                                aTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAA‑TACAAAAACTGGAGTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTc                                                                      <  1:529147/151‑1 (MQ=255)                                                                                  tACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAA‑TACAAAAACTGGAGTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTC‑g                                                                    <  2:686378/150‑1 (MQ=255)                                                                                     atatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAA‑TACAAAAACTGGAGTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTC‑ACTaa                                                                <  1:498383/151‑1 (MQ=255)                                                                                     atatTCACGGTGGCAAAAAATATAAAACCACATTTCTAGTGGTAGTTTGTGGCGGT‑GGAATTTTTCAGACAAA‑AAAAAAAAATGGAGTTGCCATGATGAGGGGAAGGAAAAAAGCAA‑AAGCAGTGAAAAAGGTTAAAGAGAATGttggt                                                                   >  2:123896/1‑145 (MQ=255)                                                                                       atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GGAATTTTCCAGACAAA‑AACAAAAACCGGAGGTGCCCAGATGG‑GGGGAAGTAATAAGCAACAAGCAGTGGAAAAGTTGACAGAGGTTTTC‑GCTa                                                                 >  2:276186/1‑148 (MQ=255)                                                                                       atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAA‑TACAAAAACTGGAGTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTC‑GCTAACt                                                              <  2:105168/151‑1 (MQ=255)                                                                                           aCGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAGACAAA‑AAAAAAAAATGGAGTTGCCATGATGA‑GGGGAAGTAAAAAACAACAAGCAGTGAATAAGATGACAGAGATTTTC‑GCTAACttt                                                            >  2:279473/1‑149 (MQ=255)                                                                                            cGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAAATTTTTCAAACAAA‑AAAAAAAACTGGAGGTGCCATGATGA‑GGGGAAAGAAAAAACAAAAAGGAGAGAATAAATTGGCAAAGAATGTC‑GCTAACttt                                                            >  2:495102/1‑148 (MQ=255)                                                                                              gTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAAATTTTTTAAACAAA‑AAAAAAAAATGGGGGTTGCATGGTGG‑GGGAAAGTAATAAAAAAAAAGCAGAGAATAAATTGAAAGAGAATGTT‑GCTAAAttt                                                            >  1:8862/1‑146 (MQ=255)                                                                                                   aaaaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAAAAAAA‑AAAAAAAAAAGGAGTTGCCATGATGA‑GGGAAAAGAATAAAAAAAAAGGAGTGAAAAAAATGACAGAGAATGTC‑GCTAACTTTAACGcc                                                      >  2:31902/1‑147 (MQ=255)                                                                                                     aaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAGACAAA‑AAAAAAAAACGGAGTTGCCATGATGG‑GCGGAAGGAATAAAAAAAAAGCAGTGAATAAAATGACAGAGATTTTC‑GCTAACTTTACCCCCCTGAtt                                                >  2:251247/1‑151 (MQ=255)                                                                                                       aaTAAAAAACCACATTTTTAGTGGTATTTTGTGGCGGT‑GAATTTTTCCAGACAAA‑TACAAAAACTGGAGTTGCAATGATGA‑GCAAAAGTAATAACCAACAGGCAGTAAATAAGTTGACAGAGTTTGTC‑GCTAACTTTACCGCCATGATTTc                                              <  1:343225/151‑1 (MQ=255)                                                                                                            aaaaCCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCCGACAAA‑AAAAAAAAATGGAGGTGCCATGATGA‑GCGAAAGTAATAAACAAAAGGCAGGGAAAAAGATGTAAGAGATTGTA‑GCTAAATTTACCGCCATGATTTTTAccc                                         >  2:220851/1‑151 (MQ=255)                                                                                                             aaaCCACATTTTTAGTGGTAGTTTGTGGCGGT‑GGATTTTTTCAGACCAA‑AAAAAAAAATGGAGGTGTCCTGGTGG‑GCGGAAGTAAAAAACAACAAGGCGTGAATAAAATTACAGAGAGTGTT‑GCCAACTTTTCCGCCATGAttttt                                             >  1:462382/1‑144 (MQ=255)                                                                                                              aaCCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCCGAAAAA‑AACAAAAAATGGAGTTGTCATGGTGA‑GGGGAAGTAATAAAAAAAAGGGAGGGAATAAAATGACAGAGATTGTC‑GCTAAATTTACCCCCACGATTTTTAacc                                         >  1:672620/1‑149 (MQ=255)                                                                                                                     tttttAGTGGTAGTTTGTGGCGGT‑GGATTTTTTCCGACAAATTACAAAAAAGGGGGTTG‑CTTGGAGG‑GGGAAAGGAATAAGAAAAAAGCGGTGAATAAATTTACAGAGATTGTCTGCAAACTTT‑TCGCCATGATTTTTATCCCAATGcc                                  >  1:79832/1‑149 (MQ=255)                                                                                                                     ttttaAGTGGTAGTTGGTGGCGGT‑GAATTTTTCCAGAAAAA‑TACAAAAACTGGATTTGCCAGGATGA‑GCGAAAGTAATAAACAACAGGCAGTGAATAAGTTGACAGAGATTGTC‑GCTAACTTTACCGCCATGATTTCTACCCGAATGCCt                                 <  2:177314/150‑1 (MQ=255)                                                                                                                      ttttAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAA‑TACAAAAACGGGAGTTGCCATGAGGA‑GCAAAAGAAATAACCAACAGGAAGTGAATAAGTTAACAGAGATTGTC‑GCTAACTTTACCGCCATGATTTCTACCCGAATGCCtg                                <  1:523627/150‑1 (MQ=255)                                                                                                                      tttaAGGGGTAGTTTTTGGCGGT‑TAATTTTCCCAGAAAAA‑TCCAAAACCTGGAGTTCCCATGAGGA‑GCGAAATAAATAAGCACCGGGTAGTGAATAAGTTGACAGAAATTGTC‑GCTAACTTTACCGCCATGATTTCTACCCGAATGCCtg                                <  2:679767/150‑1 (MQ=255)                                                                                                                       tttAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAGACAAA‑AACAAAAAATGGAGTTGCCATGATGA‑GCGAAAGTAATAAAAAAAAAGGAGGGAATAAAATGAAAGAGATTGTC‑GCTAACTTTATCGCCCCGATTTTTACCCCAATGCCtgat                              >  2:364775/1‑151 (MQ=255)                                                                                                                           gTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGAAAAA‑TACAAAAACTGGAGTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTC‑GCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGAc                           <  2:476985/150‑1 (MQ=255)                                                                                                                             ggTAGTTTGTGGCGGT‑GATTTTTTCCAGAAAAA‑TAAAAAACCGGGTTGTGCCATGATGA‑GGGAAATTAGTAAGCAACGGGCAGTAAATAGTTTAACAGAGTTTGGC‑GCTACCTTTACCGCCATGATTTCTACCCGAATGCCTGATGAc                           <  1:54453/148‑1 (MQ=255)                                                                                                                                         cGGT‑GAATTTTTCCAGACAAA‑TACAAAAACTGGAGTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTC‑GCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACt             >  1:326235/1‑150 (MQ=255)                                                                                                                                                    ttCCAGAAAAA‑TACAAAAACTGGAGTTGCCATGATGA‑GCAAAAGTAAAAAGAAACAGGCAGTAAATAAGTTGACAGAGATTGTC‑GTTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTa   <  1:284037/150‑1 (MQ=255)                                                                                                                                                      ccAGAAAAA‑TACAAAAACTGGAGTTGCCATGAGGG‑GCGAAAGTAATAAGCAACAGGCAGTGAATAATTGGACAGAGATGGTC‑GCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTaa  <  2:88985/149‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                        GTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAA‑TACAAAAACTGGAGTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTC‑GCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAA  >  NC_000913/3206287‑3206583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑