Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,508,657	0	A	G	44.7%	67.7 / 10.0	47	F386S (TTC→TCC)	yhfW	phosphopentomutase‑related metalloenzyme superfamily protein
Reads supporting (aligned to +/- strand):  ref base A (5/21);  new base G (21/0);  total (26/21)
Fisher's exact test for biased strand distribution p-value = 5.28e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATCCGC  >  NC_000913/3508508‑3508805                                                                                                                                                      |                                                                                                                                                     cAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGaa                                                                                                                                                     <  2:496183/151‑1 (MQ=255)   gTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGCGCGGgaa                                                                                                                                                    >  2:609137/1‑150 (MQ=255)     ccACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGaaaaa                                                                                                                                                  >  2:564902/1‑150 (MQ=255)        cGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGCGGCGCGGGGGAAAAATc                                                                                                                                               >  2:127904/1‑150 (MQ=255)         gttgttTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTcac                                                                                                                                             <  2:526878/151‑1 (MQ=255)           tgttTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGGGCGGgaaaaat                                                                                                                                                >  1:31456/1‑144 (MQ=255)           tgttTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTcaca                                                                                                                                            <  1:678231/150‑1 (MQ=255)                  aaaGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGGAAAATTcaaacaaag                                                                                                                                       >  2:416224/1‑145 (MQ=255)                   aaGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGGAAAATTCACACACGGTa                                                                                                                                     >  2:752970/1‑149 (MQ=255)                    aGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGGAAAAATCACACACGGTAGcc                                                                                                                                  >  1:17551/1‑151 (MQ=255)                     gCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGCGGGGGAAAAATTACAAACCGTAGacc                                                                                                                                 >  2:616883/1‑151 (MQ=255)                      ccGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGGAAAAATCACACACGGTAGcccc                                                                                                                                >  1:234329/1‑151 (MQ=255)                                cAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGCGCGGGAAAAATTACACACCGGAGccccccc                                                                                                                             >  1:786098/1‑142 (MQ=255)                                 aaTATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGaaa                                                                                                                     <  2:35485/151‑1 (MQ=255)                                  atatGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAAATTAAACACGGTAGCCCCcaccatagaaa                                                                                                                     >  1:539761/1‑142 (MQ=255)                                   tatGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGGAAAAATCACACACGGGAGCCCCCCCATCAGaaa                                                                                                                     >  2:753084/1‑149 (MQ=255)                                    atGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGCGCGGGAAAAATCACACACCGGAGGCCCCCCATCAGaaa                                                                                                                     >  2:240805/1‑148 (MQ=255)                                      gCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGGGGGGGAAAAATTACACACGGGAGCCCCCACATCAGAAAGCggg                                                                                                                >  2:30253/1‑149 (MQ=255)                                        aGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGGGGGAAAAATTAAACACCGGAGACCCCCCATccaaaaacgggg                                                                                                               >  2:27077/1‑139 (MQ=255)                                        aGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGGAAAATTTACACACGGTAGCCCCCCCATCCGAAAACgtgg                                                                                                               >  2:582616/1‑150 (MQ=255)                                          aTGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTgc                                                                                                            <  2:137985/151‑1 (MQ=255)                                          aTGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTgc                                                                                                            <  2:587568/151‑1 (MQ=255)                                             cTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGCGCGGGAAAAATTAAAAACGGTAGCCCCCACAACAGAAAACGGGGGgcgc                                                                                                          >  2:480662/1‑150 (MQ=255)                                                  gggCGAGGGTGTAACCCGTCGGATCAAAATTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCg                                                                                                    <  1:664907/151‑1 (MQ=255)                                                   ggCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAATAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGa                                                                                                   <  1:427100/151‑1 (MQ=255)                                                       aGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTg                                                                                               <  1:470469/151‑1 (MQ=255)                                                         ggTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGGAAAAATCACACACCGGAGCCCCCCCCTCAGAAAACGTGGTGCGCACACCCAGCTGc                                                                                              >  2:695581/1‑150 (MQ=255)                                                            gtAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAg                                                                                          <  2:218462/151‑1 (MQ=255)                                                                                tCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGGAAAAATTACACACGGTAGCCCCCCCATCAGAAAGCGGGGGGCGCACACCCAGCTGCGTAGCGATCATCCCtt                                                                              >  2:372874/1‑143 (MQ=255)                                                                                tCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGGAAAAATTACAAACGGGAGCCCCCCCAACAGAAAACGGGGGGGGCACACCCAGCTGCGCAGCGGTCATccc                                                                                >  1:96277/1‑141 (MQ=255)                                                                                 cATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGCGCGGAAAAAATTAAAAACGGGAGCCCCCCCATCAGAAAACGGGGGGGGGACACCCAGGTGCGGAGCGACCATCCCCTGc                                                                            >  2:382927/1‑144 (MQ=255)                                                                                 cATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGGAAAATTCACACAAGGTAGCCCCCACATCAGAAAACGTGGTGCGCCCACCCAGCTGCGGAGCGATCATCCCCTGCCGGTaa                                                                      >  2:91712/1‑150 (MQ=255)                                                                                  aTAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAAc                                                                    <  2:659733/151‑1 (MQ=255)                                                                                                  aaaCCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGGAAAAATTACACACCGGAGCCCCCACATCAGAAAGCGGGGGGCGCACACCCAACTGCGTAGCGAAAATACCTTGCTGATAAAAAAACACTGGcaccac                                                     >  2:385891/1‑150 (MQ=255)                                                                                                    aCCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTc                                                  <  1:109452/151‑1 (MQ=255)                                                                                                       ggagggagGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTcgcg                                               <  2:801450/151‑1 (MQ=255)                                                                                                        gagggaggAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCgg                                              <  2:255532/151‑1 (MQ=255)                                                                                                        gagggaggAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCgg                                              <  2:755655/151‑1 (MQ=255)                                                                                                                aaagaaaagaGCGACCATTTTGTGGCGGTGGCGCGCGGGAAAAATCACACACCGTAGCCCCCCCCTCAGAAAACGGGGGGCGCACACCGAGCTGCGTAGCGATCATCCCCTGCTGATAAAACAACACTGGCACCACCTCGCGGGGAtgg                                        >  2:155019/1‑149 (MQ=255)                                                                                                                   gaaaagaGCGACCATTTTGTGGCGGTGGCGCGCGGGAAAAATCACACACGGGAGCCCCCCCATCAGAAAGCGGGGGGCGCACACCCAGCCGCGTAGCGATCATCCCCTGCTGATAAAACAGCACTGGCACCACCTCCCGGGGAtggtgg                                     >  1:144035/1‑149 (MQ=255)                                                                                                                   gaaaagaGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCt                                   <  1:266297/151‑1 (MQ=255)                                                                                                                          gCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCa                            <  1:607321/151‑1 (MQ=255)                                                                                                                              ccATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATgg                        <  2:179592/151‑1 (MQ=255)                                                                                                                                aTTTTGTGGCGGTGGCGCGCGGGAAAATTCACACACGGGAGCCCCCACATCAGAAAGCGTGGGGCGCACACCCAGCTGCGTAGCGATCATCCCTTGCTGATAAAACAGCACTGGCACCACCTCGCGGGGATGGGGGCTGTGACCAATg                         >  2:22283/1‑148 (MQ=255)                                                                                                                                              gcgcgcGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTg        <  1:233239/151‑1 (MQ=255)                                                                                                                                                gcgcgGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGAt      >  2:647494/1‑151 (MQ=255)                                                                                                                                                gcgcgGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGAt      <  2:267760/151‑1 (MQ=255)                                                                                                                                                 cgcgGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATc     >  1:826484/1‑151 (MQ=255)                                                                                                                                                  gcgGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATcc    <  1:7887/151‑1 (MQ=255)                                                                                                                                                    ggAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATCCGc  >  2:112168/1‑151 (MQ=255)                                                                                                                                                    ggAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGCCACCACTTCGCGGGTATGGTGGCTGTGCCCAATGGTCGGATCGTTGCCGTGATCCGc  <  1:125808/151‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                     CAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATCCGC  >  NC_000913/3508508‑3508805

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑