| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,042,993 | 0 | C | T | 60.0% | ‑5.3 / 17.9 | 26 | F193F (TTC→TTT) | srkA | Cpx stress response Thr/Ser protein kinase; MazF antagonist protein |
| Reads supporting (aligned to +/- strand): ref base C (8/2); new base T (15/0); total (24/2) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.50e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.05e-01 | |||||||||||
CCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGAT > NC_000913/4042847‑4043142 | ccGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGGAGATTTTAc > 2:715644/1‑149 (MQ=255) ccATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGAATTTAcccgtct > 2:689414/1‑146 (MQ=255) ccATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAAATTTACCCGtt > 2:706389/1‑149 (MQ=255) gTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCCTTTTGCgg > 2:30122/1‑149 (MQ=255) tttGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCgg > 2:125730/1‑148 (MQ=255) gAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTTCCGCTACa > 1:721939/1‑146 (MQ=255) gAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTGCGGCTACATgg > 2:67409/1‑149 (MQ=255) tatCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGAGTTTACCCCTCTGCGGCCACATgggg > 2:163785/1‑146 (MQ=255) atCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGGAGATATCCCCCTTTTGCGGCATCCTGgagaa > 2:669469/1‑148 (MQ=255) atCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTTTCGATCTGCGGCTACAAggggcccg > 1:817034/1‑145 (MQ=255) ctcATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCCTTCTGCGGCTACATGGAGACTTcc > 2:300856/1‑151 (MQ=255) tGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGAATTTACCGGTTTGCGGCTATACGGCGACCGc > 1:281103/1‑145 (MQ=255) gTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGGATATtttc > 1:660206/1‑148 (MQ=255) gTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTg < 2:754461/150‑1 (MQ=255) tACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGCTTTTACCGTTCTGCGGGTACATTGAGAATGCCACGCCGGGGATATTTTCTTGcgcggggggac > 1:473240/1‑143 (MQ=255) ccGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTAACGTTCCTCGGGTACATGGAGACTGCCCCACCCGGGAAATTTTCTTGCGCGATGGTCCCATGTTTGTTGAttt > 1:327473/1‑149 (MQ=255) tGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGGAGGTTTTACCGGTCTTCGGCTACAAGGGGACCGCCACGCCGGGAATATTTTCTGGGGCGGTGGGCCAATGGTTGTTTATCTGggtg > 2:182361/1‑150 (MQ=255) gAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCCTTTTGCGGCCACACGGAGACTGCCACGCCGGGGATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGggtg > 2:530645/1‑149 (MQ=255) aaaGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTGCGGCTACATGGGGACTGCCCCCCCGGGGATATTCTCTGGCGCGGTGGTCCAATGTTTTTTGATCTGggtgat > 2:64537/1‑150 (MQ=255) gATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTtgt < 1:531744/149‑1 (MQ=255) ccgTTACAGCACACTGGCGGGAAGATTTTACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGGATATTTTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGGTGATGCACGGAATGGGCCAGCCGTTCAGGGTTTTTGGATGTTg > 2:225488/1‑149 (MQ=255) cgTTACAGCACACTGGCGGGGAGAATTTACCGTTCTGCGGCTACATTGAGGCCGCCACGCCGGGGATATTTTCCGGCGCGGTGGTCCACTGTTTGTTGTTTTGGGTTAAGCCCGCAATGGTCCCGCCGTTCTGGATTTTTGGGTGTTtctc > 1:221133/1‑151 (MQ=255) cgTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGGGACCGCCCCGCCGGGGATATTCTCTGGCGCGAAGGGCCAATGTTTGTTTGTCTGGGTGATGCACGTAATGGTACAGCCCGTCAGGGTTTTTGGATGTTGCTc > 2:212283/1‑151 (MQ=255) ggAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCg > 1:251828/1‑149 (MQ=255) gATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGagcagc > 2:245973/1‑151 (MQ=255) tCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGAt > 1:677166/1‑151 (MQ=255) | CCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGAT > NC_000913/4042847‑4043142 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |