Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913343,4440AG45.0% 49.0 / 13.3 40G187G (GGA→GGG) yahKbroad specificity NADPH‑dependent aldehyde reductase, Zn‑containing
Reads supporting (aligned to +/- strand):  ref base A (1/21);  new base G (18/0);  total (19/21)
Fisher's exact test for biased strand distribution p-value = 1.68e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGTTCTGCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGCCCTGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCGATGAGATGGCGGCTCATCTGAAGAGT  >  NC_000913/343300‑343594
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tGTTCTGCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGGCCGGGGCatct                                                                                                                                                   >  1:409668/1‑148 (MQ=255)
  ttCTGCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGGCTGGGGCATATggg                                                                                                                                                >  1:253737/1‑151 (MQ=255)
    cTGCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGGATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATggg                                                                                                                                                <  1:366649/149‑1 (MQ=255)
      gCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGAtt                                                                                                                                            <  1:174011/151‑1 (MQ=255)
          aTTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGGCTGGGGCATATggggggtta                                                                                                                                          >  1:387720/1‑144 (MQ=255)
                           gcagcTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGCGATggg                                                                                                                        <  1:828470/150‑1 (MQ=255)
                              gcTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGGCTGGGGCATATGGGGGGTTAGGGGGGCCCCGCGGTggggg                                                                                                                      >  1:443685/1‑149 (MQ=255)
                                    ggcggTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGGCTGGGGCATATGGGGGGTAAGCTGGCCCCCGCGATGGGGGcaca                                                                                                                  >  1:130022/1‑147 (MQ=255)
                                             tCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGGCATATGGGGGTTAAGTTGGGCCACGCGGTGGGGGGACATGTGGGGGGAttt                                                                                                     >  1:190996/1‑151 (MQ=255)
                                                     tgtgtgCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGGCATATGGGGGTTAAACTGGCCCACGCGATGGGGGGACATGTGGGGGCATTTACCACtt                                                                                              >  2:3211/1‑150 (MQ=255)
                                                          gCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAgg                                                                                        <  2:54276/151‑1 (MQ=255)
                                                            aGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGGCATATGGGGGTTTAACTGGCCCACGCGATGGGGGCCCATGGGGGGGCCTTTTCCACCTCTgggg                                                                                        >  1:618388/1‑149 (MQ=255)
                                                             gggATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCaa                                                                                     <  1:301747/151‑1 (MQ=255)
                                                               gATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCaaaa                                                                                   <  1:797406/151‑1 (MQ=255)
                                                                aTCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGGCATATGGGGGTTAAGCTGGCCCACGCGATGGGGGCACATGTGGGGGGCTTTACCACTTCTTGGGCaaaa                                                                                   >  2:145330/1‑150 (MQ=255)
                                                                   accacGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAAcgcg                                                                               <  1:189150/151‑1 (MQ=255)
                                                                     cacGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGGCATATTGGGGTTAAGCCGGCCCCCGCGGTGGGGGGACATGGGGGGGCCTTTACCACTTCTGAGGCAAAAAGCGa                                                                              >  1:649581/1‑150 (MQ=255)
                                                                      acGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGGCATATGGGGATTAAGCTGGCCCACGCGATGGGGGCACATGTGGTGGCATTTACCACTTTTGAGGCAAAAagcg                                                                               >  2:509372/1‑148 (MQ=255)
                                                                          aTTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCgg                                                                         <  2:771251/150‑1 (MQ=255)
                                                                          aTTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGc                                                                        <  2:298571/151‑1 (MQ=255)
                                                                             cgccgcTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGGCATATGGGGGTTAAGCTGGCCCACGCGGTGGGGGCACATGTGGGGGCATTTAACACTTTTGAGGCAAAAAGCGAAGCGGGaa                                                                      >  2:363947/1‑150 (MQ=255)
                                                                             cgccgcTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGGCATATGGGGGGTAAGGCGGCCCCCGGGATGGGGGGACATGTGGGGGGATTTACCACTTTTGAGGCAAAACGCGAAGCGGCaa                                                                      >  1:401305/1‑150 (MQ=255)
                                                                              gccgcTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCaaaa                                                                    <  1:175494/151‑1 (MQ=255)
                                                                              gccgcTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGGCTGGGGCATATGGGGGTTAAAGTGGCCCACGCGCTGGGGGCACATGTGGGGGGATTTTCCACTTTTGTGGCAAAAAGCGAAGGGGCaaa                                                                     >  1:191671/1‑150 (MQ=255)
                                                                                 gcTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGGCATATGGGGGTTAAGCTGGCCCACGCGATGGGGGCACATGTGGGGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGcc                                                                 >  1:461204/1‑151 (MQ=255)
                                                                                  cTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGGCATATGGGGGTTAAACTGGGCCCCGCGATGGGGGGACCTGTGGTGGGATTTACCACTTCTGAGGGAAAACGCGAAGAGGCAAAAGccc                                                                >  1:250867/1‑151 (MQ=255)
                                                                                    aCGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGCCCt                                                               <  1:159391/150‑1 (MQ=255)
                                                                                           tGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGGCATATGGGGATTAAGCTGGGCCACGCGGTGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGCCCCtgggg                                                          >  1:256223/1‑148 (MQ=255)
                                                                                            ggCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGCCCTGGGGGCCg                                                       <  2:154314/150‑1 (MQ=255)
                                                                                               aGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGGCATATGGGGGTTAAGCTGGCCCACGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGCCCTGGGGGGCGATg                                                    >  1:351066/1‑150 (MQ=255)
                                                                                                   cgggccgggTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGCGATGGGGGCACATGTGGTGGCAGTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGCCCTGGGGGCCGATGAAgt                                                <  1:306913/150‑1 (MQ=255)
                                                                                                    gggccgggTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGCCCTGGGGGCCGATGAAgttg                                              <  2:680453/151‑1 (MQ=255)
                                                                                                             aaaaaaGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGCCCTGGGGGCCGATGAAGTTGTTAACTCAc                                     <  1:430486/151‑1 (MQ=255)
                                                                                                              aaaaaGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGCCCTGGGGGCCGATGAAGTTGTTAACTCACg                                    <  2:191011/151‑1 (MQ=255)
                                                                                                               aaaaGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGCCCTGGGGGCCGATGAAGTTGTTAACTCACGc                                   <  2:528944/151‑1 (MQ=255)
                                                                                                                      ggCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGCGATGGGGGCACATGTGGGGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGCCCTGGGGGCCGATGAAGGTGTTAACTCACGCAAAGcc                             >  2:113395/1‑150 (MQ=255)
                                                                                                                                gCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGCCCTGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCGATGAGATggc                  <  2:424161/151‑1 (MQ=255)
                                                                                                                                    cggcggTCTGGGACATATGGGGATTAAGCTGGCCCACGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGCCCTGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCGATGAGATGGCGGCt              <  2:454243/151‑1 (MQ=255)
                                                                                                                                             gggACATATGGGGATTAAGCTGGCCCACGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGCCCTGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCGATGAGATGGCGGCTCATCTg        <  1:557193/148‑1 (MQ=255)
                                                                                                                                             gggACATATGGGGATTAAGCTGGCCCACGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGCCCTGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCGATGAGATGGCGGCTCATCTGAag     <  2:496764/151‑1 (MQ=255)
                                                                                                                                                aCATATGGGGATTAAGCTGGCCCACGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGCCCTGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCGATGAGATGGCGGCTCATCTGAAGAGt  <  1:70489/151‑1 (MQ=255)
                                                                                                                                                aCATATGGGGATTAAGCTGGCCCACGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGCCCTGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCGATGAGATGGCGGCTCATCTGAAGAGt  >  2:799286/1‑151 (MQ=255)
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TGTTCTGCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGCCCTGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCGATGAGATGGCGGCTCATCTGAAGAGT  >  NC_000913/343300‑343594

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑