Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	354,288	0	T	G	50.0%	31.1 / 24.5	42	V528G (GTG→GGG)	prpE	propionate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base T (4/17);  new base G (21/0);  total (25/17)
Fisher's exact test for biased strand distribution p-value = 4.70e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGC  >  NC_000913/354146‑354437                                                                                                                                               |                                                                                                                                                      aTCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGa                                                                                                                                                 >  2:239618/1‑149 (MQ=255)  tCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGGTgg                                                                                                                                              >  1:780384/1‑150 (MQ=255)         cGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTAGCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGaaa                                                                                                                                       <  2:371214/151‑1 (MQ=255)                   tATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAggtg                                                                                                                              <  2:823419/150‑1 (MQ=255)                          ttATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCTACCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAggtggcggtgg                                                                                                                       <  2:580826/150‑1 (MQ=255)                          ttATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGGAAGGTGCGCTGGAAGGGCAgggggcggggg                                                                                                                       >  2:130820/1‑150 (MQ=255)                            aTTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGGAAgggggggggggggggggg                                                                                                                     >  1:497323/1‑135 (MQ=255)                                 cGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGttt                                                                                                                  <  2:522987/148‑1 (MQ=255)                                  gggCGCACTGGCGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTAGCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGtt                                                                                                                   <  1:54026/146‑1 (MQ=255)                                      gcACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGGAAGGGGAGGGGGGGGGGGGGGTTGTTAtt                                                                                                            >  1:643847/1‑149 (MQ=255)                                           gACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGaa                                                                                                       <  1:425731/149‑1 (MQ=255)                                                      tAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGGAAGATGCGCTGAAAAGGCAGGGGGGGGGGGGGGTTGGCATTCCGAAAGAGAGCGGCa                                                                                            >  2:122623/1‑149 (MQ=255)                                                      tAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGAAGGGCTGGAAAGGCAGGGGGCGGGGGGGGTTGGCATTcc                                                                                                          >  1:190648/1‑135 (MQ=255)                                                       aaCGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGGAAGATGCGCTGCAAAGGCAGGGGGCGGGGGCGGTTGTCATTCCGAAAGAGAGCg                                                                                               >  2:644219/1‑145 (MQ=255)                                                         cGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTg                                                                                       <  1:791685/151‑1 (MQ=255)                                                            tGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGAAGCGCTGGAAGGGCAGGGGGCGGGGGCGGTTGTCATTCCGAAAGAGAGCGACAGTCTgg                                                                                      >  2:468505/1‑149 (MQ=255)                                                              ccGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGGAAGAAGCGCCGAAAGGGGAGGGGGCGGGGGCGGTTGTTATTCCGAAAGAGAGGGGCAGt                                                                                          >  2:733016/1‑143 (MQ=255)                                                                   cATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTg                                                                             <  2:406393/151‑1 (MQ=255)                                                                   cATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTg                                                                             <  1:172258/151‑1 (MQ=255)                                                                   cATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGAAAAGGGAGGGGGCGGGGGGGGTTGTCATTTCGAAAGAGAGCGACAGTCTGGaa                                                                                    >  2:283180/1‑144 (MQ=255)                                                                       ggCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGAtgt                                                                         <  2:525950/151‑1 (MQ=255)                                                                       ggCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGCTGAAAGGGCAGGTGGGGGGGGGGGTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTgg                                                                            >  2:107317/1‑147 (MQ=255)                                                                          tGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGGAAGGGGAGGGGGCGGGGGGGGTTGTCATTCCGAAAGAGAGGGACAGGTTGGGAGATTGGGAGGTggg                                                                      >  1:554184/1‑150 (MQ=255)                                                                           ggggACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGgcg                                                                     <  2:497357/151‑1 (MQ=255)                                                                             ggACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCa                                                                   <  2:580657/151‑1 (MQ=255)                                                                             ggACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCa                                                                   <  1:35547/151‑1 (MQ=255)                                                                                cgcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGCTGGAAGGGGAGGGGGGGGGGGGGGTTGTTATTTCGAAAAAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACt                                                                 >  2:2352/1‑150 (MQ=255)                                                                                  cgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGATGGGGTGAAAGGGCAGGGGGGGGTGGGGTTTTGTATTCCGAAAGAGAGCGACCGGATGGAAGATCGGGATGTTGCGCACTCGc                                                              >  1:198570/1‑151 (MQ=255)                                                                                   gcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCa                                                             <  2:582373/151‑1 (MQ=255)                                                                                    cgAGATTTAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGTGAAAGATGCGCTGCAAGGGCCGGGGGGGGTGGGGTTTTTCATTCCGCAAGAGAGGGACAGTCTGGTAGAGCGTGGTGGGGGGCTCTTGc                                                              >  1:580748/1‑149 (MQ=255)                                                                                    cgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGTGGAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGGTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGGTGTGGCGCACTc                                                                >  2:417504/1‑147 (MQ=255)                                                                                        aTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGGAGGGGGCGGGGGCGTTTGTCCTTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGGACTCGCaa                                                            >  2:289806/1‑147 (MQ=255)                                                                                           gAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAgg                                                     <  1:256885/151‑1 (MQ=255)                                                                                           gAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAg                                                      <  1:492060/150‑1 (MQ=255)                                                                                           gAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGAAGCGCTGGAAGGGGAGGGGGCGGGGGGGGTTGTCATTCCGGAAGAGAGCGGCCGTCTTGGAGATCGTGATGTGGCGCACCCCCAAGAGAAgg                                                     >  1:95318/1‑151 (MQ=255)                                                                                               agagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGAAAAGGGAGGGGGCGGGGGGGGTTGTCATTCCGAAAAAGAGCGACAGTCTGGGAGAACGTGATGTGGCGCACTCCCAAGAGaa                                                       >  2:472056/1‑145 (MQ=255)                                                                                                 agTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGAAAGGGCAGGGGGCGGGGGCGGTTGTCATTTCGAAAGAGAGCGACAGTCTGGGAGATCGGGATGTGGGGCCCTCGCCAGAGAAGGCGAtt                                                >  1:813700/1‑150 (MQ=255)                                                                                                 agTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCCGGAAGGGCAGGGGGCGGGGGCGGTTGTCATTTCGAAAGAGAGGGACAGGCTGGGAGATCGTGGTGTGGGGCACTCGCAAGAGAAGGCGGtt                                                >  1:786581/1‑150 (MQ=255)                                                                                                            ccATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGAAGCGCTGAAAGGGCAGGGGGCGGGGGCGGTTTTCATTCCGAAAGAAAGCGACAGTCTGGGAGATCGAGGTGTGGCGCACTCGCAAGAGAAGGGGATTATTGCGCt                                        >  2:650055/1‑147 (MQ=255)                                                                                                              aTCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGGAAGGGGGGGGGGCGGGGGCGGTTGTCCTTCCGGAAGAGAGCGACAGGCTGGAAGATCGGGATGGGGGGCCCTCGCCAGAGAAGGGGGTTATGGCGCtggtgg                                   >  1:241005/1‑150 (MQ=255)                                                                                                                                        gtTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGgccgcc        <  2:228476/151‑1 (MQ=255)                                                                                                                                              gTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGgcgc  >  1:330238/1‑151 (MQ=255)                                                                                                                                               |                                                                                                                                                      ATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGC  >  NC_000913/354146‑354437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑