Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,186,274	A→T	N309Y (AAC→TAC)	rpoC →	RNA polymerase, beta prime subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,186,274	0	A	T	100.0%	181.1 / NA	46	N309Y (AAC→TAC)	rpoC	RNA polymerase, beta prime subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (24/22);  total (24/22)

CGCGACTTCTGACCTGAACGATCTGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATC  >  NC_000913/4186129‑4186414                                                                                                                                                  |                                                                                                                                             cgcgACTTCTGACCTGAACGATCTGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATTACGgt                                                                                                                                         >  1:283032/1‑151 (MQ=255)  gcgACTTCTGACCTGAACGATCTGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATTACGgtc                                                                                                                                        <  2:735541/151‑1 (MQ=255)   cgACTTCTGACCTGAACGATCTGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATTACGgtcg                                                                                                                                       <  2:762528/151‑1 (MQ=255)      cTTCTGACCTGAACGATCTGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATTACGGtcgtcg                                                                                                                                    >  1:544042/1‑151 (MQ=255)         cTGACCTGAACGATCTGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATTACGGTCGTCGCgg                                                                                                                                 >  2:403470/1‑151 (MQ=255)                gAACGATCTGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATTACGGTCGTCGCGGTCGTGc                                                                                                                           <  2:246753/150‑1 (MQ=255)                gAACGATCTGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATTACGGTCGTCGCGGTCGTGc                                                                                                                           <  2:459053/150‑1 (MQ=255)                         gTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATTACGGTCGTCGCGGTCGTGCGATCAc                                                                                                                     <  1:136092/147‑1 (MQ=255)                         gTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATTACGGTCGTCGCGGTCGTGCGATCACCGGt                                                                                                                 <  1:608235/151‑1 (MQ=255)                            tcgtcgCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATTACGGTCGTCGCGGTCGTGCGATCa                                                                                                                      <  2:279802/143‑1 (MQ=255)                             cgtcgCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATTACGGTCGTCGCGGTCGTGCGATCACCGGTTCt                                                                                                              >  1:622529/1‑150 (MQ=255)                                cgcgTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATTACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAAc                                                                                                           >  1:170426/1‑150 (MQ=255)                                 gcgTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATTACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAACa                                                                                                          >  2:481020/1‑150 (MQ=255)                                      aTTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATTACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAACAAGCGTc                                                                                                    <  1:451151/151‑1 (MQ=255)                                           ccGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGCAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATTACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTg                                                                                               >  2:623026/1‑151 (MQ=255)                                              tAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATTACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGaa                                                                                             >  2:190456/1‑150 (MQ=255)                                               aacaacCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATTACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAAt                                                                                           <  2:378003/151‑1 (MQ=255)                                                    ccGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATTACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTg                                                                                      <  2:27151/151‑1 (MQ=255)                                                          gAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATTACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGcc                                                                                   <  1:793373/148‑1 (MQ=255)                                                             aCGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATTACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATg                                                                             <  2:101512/151‑1 (MQ=255)                                                                  tgctgGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATTACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCa                                                                         >  1:156650/1‑150 (MQ=255)                                                                    ctgGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATTACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCaa                                                                        >  1:559174/1‑149 (MQ=255)                                                                     tgGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATTACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAgg                                                                     >  2:413375/1‑151 (MQ=255)                                                                      ggATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATTACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGt                                                                    <  1:796309/151‑1 (MQ=255)                                                                       gATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATTACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAgg                                                                     >  1:613584/1‑149 (MQ=255)                                                                                gcgcCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATTACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAggg                                                            <  1:817538/149‑1 (MQ=255)                                                                                  gcCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATTACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCg                                                         <  2:597995/150‑1 (MQ=255)                                                                                      gACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATTACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTcc                                                    >  1:637786/1‑151 (MQ=255)                                                                                            atcGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATTACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTc                                                 >  2:823495/1‑148 (MQ=255)                                                                                             tcGTACGTAACGAAAAACGTATGCTGCCGGAAGCGGTAGTCGCCCTGCGGGATTACGGTCGTCGCGGTCGTGTGACCACCGGTTCTAACAAGCGTCCTCTGATATCTTTGGCCAACATGATCAAAGTTAAACAGGGTAGATTCCGTCAGa                                              <  1:679634/150‑1 (MQ=255)                                                                                              cgtacgtaACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATTACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAAc                                            >  2:144649/1‑151 (MQ=255)                                                                                                 acgtaACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATTACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCt                                          <  2:651861/150‑1 (MQ=255)                                                                                                 acgtaACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATTACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTg                                         >  2:463068/1‑151 (MQ=255)                                                                                                   gtaACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATTACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGc                                        <  1:69693/150‑1 (MQ=255)                                                                                                           aaaCGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATTACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGc                               <  1:452261/151‑1 (MQ=255)                                                                                                             aCGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATTACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCgt                             >  2:202081/1‑151 (MQ=255)                                                                                                               gTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATTACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCgtgt                           >  1:154816/1‑151 (MQ=255)                                                                                                                    ctgcAGGAAGCGGTAGACGCCCTGCTGGATTACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGact                      >  1:108101/1‑151 (MQ=255)                                                                                                                       cAGGAAGCGGTAGACGCCCTGCTGGATTACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCCGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGactact                   >  1:539428/1‑151 (MQ=255)                                                                                                                             gCGGTAGACGCCCTGCTGGATTACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTc             <  2:688873/151‑1 (MQ=255)                                                                                                                                gTAGACGCCCTGCTGGATTACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCGTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGGCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGtt          <  1:191591/151‑1 (MQ=255)                                                                                                                                 tAGACGCCCTGCTGGATTACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTc         >  1:543245/1‑151 (MQ=255)                                                                                                                                    aCGCCCTGCTGGATTACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGt      <  2:346206/151‑1 (MQ=255)                                                                                                                                      gCCCTGCTGGATTACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTa     >  1:710811/1‑150 (MQ=255)                                                                                                                                      gCCCTGCTGGATTACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTa     >  1:744797/1‑150 (MQ=255)                                                                                                                                        ccTGCTGGATTACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATc  >  2:363342/1‑151 (MQ=255)                                                                                                                                                  |                                                                                                                                             CGCGACTTCTGACCTGAACGATCTGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATC  >  NC_000913/4186129‑4186414

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑