Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,624,5140AT45.3% 79.1 / 37.2 64I491F (ATT→TTT) ppkpolyphosphate kinase, component of RNA degradosome
Reads supporting (aligned to +/- strand):  ref base A (9/26);  new base T (29/0);  total (38/26)
Fisher's exact test for biased strand distribution p-value = 1.19e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCACGTAAAGAAAACGGTGAAGTGGTGCGTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTATTTAACTTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTATCGCCGCAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGCTAAATAACCTTG  >  NC_000913/2624366‑2624664
                                                                                                                                                       |                                                                                                                                                        
ttCACGTAAAGAAAACGGTGAAGTGGTGCGTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTGTTTAACtttttt                                                                                                                                                        >  2:173518/1‑151 (MQ=255)
ttCACGTAAAGAAAACGGTGAAGTGGTGCGTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTATTTAACTTTAt                                                                                                                                                         <  1:171538/150‑1 (MQ=255)
ttCACGTAAAGAAAACGGTGAAGTGGTGCGTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTATTTAACTTTAt                                                                                                                                                         <  1:242264/150‑1 (MQ=255)
  cACGTAAAGAAAACGGTGAAGTGGTGCGTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTGTTTAACtttttt                                                                                                                                                        >  2:380559/1‑149 (MQ=255)
  cACGTAAAGAAAACGGTGAAGTGGTGCGTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTATTTAACTTTATTg                                                                                                                                                       <  1:651525/150‑1 (MQ=255)
  cACGTAAAGAAAACGGTGAAGTGGTGCGTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTATTTAACTTTATTg                                                                                                                                                       <  2:551078/150‑1 (MQ=255)
   aCGTAAAGAAAACGGTGAAGTGGTGCGTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTATTTAAATTTAtt                                                                                                                                                        >  2:472356/1‑148 (MQ=255)
         aGAAAACGGTGAAGTGGTGCGTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGGGTTTAACTTTTTTTGAAAAcc                                                                                                                                                 >  2:385930/1‑150 (MQ=255)
          gAAAACGGTGAAGTGGTGCGTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGGTTTTAAATTTTTTTaaaaac                                                                                                                                                 >  1:213054/1‑146 (MQ=255)
          gAAAACGGTGAAGTGGTGCGTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGGGTTTAACTTTTTTGAAAAccc                                                                                                                                                >  1:598071/1‑149 (MQ=255)
           aaaaCGGTGAAGTGGTGCGTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGGATTTTACttttttttaaaacc                                                                                                                                                >  1:542154/1‑140 (MQ=255)
           aaaaCGGTGAAGTGGTGCGTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGGATTTTACTTTTTTGaaaaac                                                                                                                                                 >  1:531715/1‑145 (MQ=255)
                 gTGAAGTGGTGCGTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTATTTTATTTTTTTTAAAAACCATAACCTcc                                                                                                                                       >  2:227703/1‑151 (MQ=255)
                  tGAAGTGGTGCGTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGGAGTTTATTTTTTTTGATAAccaaaccc                                                                                                                                           >  2:468587/1‑141 (MQ=255)
                   gAAGTGGTGCGTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTATTTAACTTTATTGAAAACCCATACCGTcc                                                                                                                                       <  1:507728/149‑1 (MQ=255)
                    aaGTGGTGCGTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTATTTAACTTTATTGAAAACCCATACCGTCCGGt                                                                                                                                    <  2:12444/151‑1 (MQ=255)
                    aaGTGGTGCGTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGGTTTTTAATTTTTTGAAAAACCAAACCGTcc                                                                                                                                       >  1:292070/1‑148 (MQ=255)
                       tggtgCGTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGGGTTTTAATTTTTTTAAAAACCATAcctgcccg                                                                                                                                     >  1:503521/1‑141 (MQ=255)
                        ggtgCGTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTATTTAACTTTATTGAAAACCCATACCGTCCGGTGACa                                                                                                                                <  1:350152/151‑1 (MQ=255)
                            cGTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGGATTTTAATTTTTTTTAAAACCATAcccccccggggcattt                                                                                                                             >  2:338641/1‑136 (MQ=255)
                             gTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGGATTTAACTTTTTTTTAAACCCCTACCACCCGGTGACAttt                                                                                                                            >  2:643616/1‑149 (MQ=255)
                                   cacacaTCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTATTTAACTTTATTGAAAACCCATACCGTCCGGTGACATTTGattat                                                                                                                      <  2:167559/149‑1 (MQ=255)
                                   cacacaTCGGGACCGAGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTATTTAACTTTATTGAAAACCCATACCGTCCGGTGACATTTGattat                                                                                                                      <  2:112852/149‑1 (MQ=255)
                                      acaTCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGGATTTTACTTTTTTTAAAAACCCATACCTCCGGGGGACATTTGTTATTTTAt                                                                                                                 >  1:598298/1‑151 (MQ=255)
                                       caTCGGGACCGGGAACTTTAACGAAAAACCCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTATTTAACTTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAAt                                                                                                                 <  1:517442/150‑1 (MQ=255)
                                       caTCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGGATTTTAATTTATTTAAAAACCCAACCGTCCCGGGGCATTTTATTAttt                                                                                                                    >  2:273654/1‑147 (MQ=255)
                                        aTCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTATTTAACTTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATgg                                                                                                               <  1:373822/151‑1 (MQ=255)
                                           gggACGGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTATTTAACTTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTAt                                                                                                            <  2:458180/151‑1 (MQ=255)
                                           gggACCGGGAACTTTAACGAAAAACCCGCGCGTCTTTATACTGACTATTCGTTGTTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTATTTAACTTTATTGAAAACCCATACCGTCCGGTGACATTTGATTAttt                                                                                                                    <  2:272305/143‑1 (MQ=255)
                                           gggACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTATTTAACTTTTTTTAAAAACCCTACCGTCCGGGGGCAATTTATTATTTTATGGt                                                                                                              >  1:815927/1‑149 (MQ=255)
                                             gACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGGATTTAACTTTATTTTAAAACCATACCCTCCCGGGGCATTTGATTATTTTATTGTTTc                                                                                                           >  2:239872/1‑150 (MQ=255)
                                                 gggAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTATTTAACTTTTTTTAAAAACCCTACCGTCCGGTGAAATTTTATTTTTTTATGGGATcgcc                                                                                                        >  1:517160/1‑149 (MQ=255)
                                                    aaCTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTGTTTAACTTTTTTTAAAAACCATACCCTCCGGGGGCATTTTTTTATTTTATGGTATtgcccc                                                                                                      >  2:534365/1‑146 (MQ=255)
                                                     aCTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTTTTTAAAATTTTTTAAAAACCAAATCTTCCGGCGCATCTTTGTTATTTAATAGTATcccccgca                                                                                                    >  2:459974/1‑145 (MQ=255)
                                                      cTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGGGTTTTACTTTTTTTAAAAACCCCAACCTCCCGGGGCATTTTGTTTTTTTTTTGTATTGCCGCaaaa                                                                                                  >  2:780733/1‑150 (MQ=255)
                                                      cTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGCATTTAACCTTTTTTTAAAACCATACCCTCCGGGGACAATTGATTTTTTAAATGTAAcgcccccaa                                                                                                   >  1:282617/1‑144 (MQ=255)
                                                          aaCGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGGGATTTACTTTTTTTTAAAACCCTAACCCCCGGTGGCATATTATTATTTTATGGTATTGCCGCAAAACAccc                                                                                             >  1:608458/1‑151 (MQ=255)
                                                                  aaCCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGGGTTTTACTTTTTTTAAAAACCCAAACGACCGGTGGGATTTTATTTTTTTATGTTATTGCCGCaaaaacccccccccc                                                                                       >  2:442703/1‑138 (MQ=255)
                                                                  aaCCGAGCGTCTTTATACTTACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTATTTAACTTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTATCGCCGCAAAACTCCCGCCGCCTa                                                                                     <  1:326155/151‑1 (MQ=255)
                                                                    ccGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTATTTAACTTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTATCGCCGCAAAACTCCCGCCGCCTAtt                                                                                   <  1:59377/151‑1 (MQ=255)
                                                                     cgcgcgTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGGTTTTAACTTTTTTTAAAACCCAAACCCTCCGGTGACATTTTTTTATTTAATTGTATCGCCGCAAAACTCCCCCCGCCTAttt                                                                                  >  1:816706/1‑150 (MQ=255)
                                                                      gcgcgTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGGATTTTACTTTTTTTAAAACCCCTACCCTCCGGGGACAATTTATTATTTTATTGTATTGCCGCCAAAATCCCCCCGCCTAtttt                                                                                 >  1:509388/1‑149 (MQ=255)
                                                                       cgcgTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTATTTAACTTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTATCGCCGCAAAACTCCCGCCGCCTATTGTa                                                                                <  1:742682/151‑1 (MQ=255)
                                                                          gTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGGATTTTACTTTTTTTAAAAACCCAACCCTCCCGTGGCATTTGTTTTTTTTATGGTATCGCCGCCAAACTCCCCCCCCCtttttttt                                                                               >  1:520584/1‑145 (MQ=255)
                                                                            cTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTATTTAACTTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTATCGCCGCAAAACTCCCGCCGCCTATTGt                                                                                 <  2:34901/145‑1 (MQ=255)
                                                                             tttATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGGGTTTATCTTTTTTTAAAAACCACACCCTCCGGGGACCTTTTTTTTTTTTATTGTATTGCCGCAAAACACCCCCCGCCCATTTTATGaaa                                                                          >  2:675935/1‑150 (MQ=255)
                                                                                        tATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTATTTAACTTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTATCGCCGCAAAACTCCCGCCGCCTATTGTATGAAATGGTGGa                                                                   <  1:239892/146‑1 (MQ=255)
                                                                                           tCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGGGTTTTACTTTTTTTAAAACCCCTACCCTTCGGAGACATTTTATTTTTTAATGGGATAGCCGCAAAAAAACCCACGCCTCTTGTATTTAAAtggtgac                                                                   >  1:134844/1‑142 (MQ=255)
                                                                                             gTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGGATTTTACTTTTTTTAAAAACCCTACCGTCCGGTGGCATTTTATTATTTAATGGTATCGCCGCAAAACTCCCGCCGCCCATTGTTTGAAATGGTGGAGcgcg                                                              >  1:251399/1‑146 (MQ=255)
                                                                                              ttGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTATTTTACTTTTTTTTAAAACCCTAACCCCCCGGGAGATTTGTTTATTTTATAGTATTTACCCAAAAATCCCCCCCCCCATTTTATGAAAAGATGGAACGCgggaaa                                                         >  2:295691/1‑148 (MQ=255)
                                                                                              ttGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTATTTAACTTTTTTTAAAACCCATACCCCCCCGGGACATTTTATTTTTTTATTGTATTGCCGCCAAACCCCCCCCGCCCATTGTATTAAATGGGGGGcc                                                                 >  2:118241/1‑142 (MQ=255)
                                                                                               tGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTATTTAACTTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTATCGCCGCAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCg                                                        <  2:551035/150‑1 (MQ=255)
                                                                                                             gcgcgcATCACCAACGAAGTACGGCGGGTATTTAACTTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTATCGCCGCAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAg                                         <  1:118260/151‑1 (MQ=255)
                                                                                                              cgcgcATCACCAACGAAGTACGGCGGGGATTTTACTTTTTTTTAAAACCCTACCCTCCGGGGGCATTTTATTATTTAATGGGAACGCCCCAAAACTCCCCCCCCCCATTTTATGAAAAGGGGGGCCGCGGGAACGCCAACCCGCCGCaa                                          >  1:143430/1‑149 (MQ=255)
                                                                                                                 gcATCACCAACGAAGTACGGCGGGTATTTAACTTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTATCGCCGCAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCt                                     <  1:780800/151‑1 (MQ=255)
                                                                                                                 gcATCACCAACGAAGTACGGCGGGGATTTTACTTTTTTTAAAACCCCTACCGTCCGGTGACATTTTTTTTTTTAATTGTATCGCCGCAAAACTCCCCCCGCCCTTTGTATGAAATGGTGGGCCGCGAGATCGCCCACGCGCCGCAAggg                                       >  1:799202/1‑149 (MQ=255)
                                                                                                                        cAACGAAGTACGGCGGGTATTTAACTTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTATCGCCGCAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGt                              <  1:579686/151‑1 (MQ=255)
                                                                                                                            gAAGTACGGCGGGTATTTAACTTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTATCGCCGCAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTa                          <  2:57359/151‑1 (MQ=255)
                                                                                                                                       gggTATTTAACTTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTATCGCCGCAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGaa                <  2:538800/151‑1 (MQ=255)
                                                                                                                                        ggTATTTAACTTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTATCGCCGCAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGa                 <  2:403804/149‑1 (MQ=255)
                                                                                                                                             tttAACTTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTATCGCCGCAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGCTa            >  1:644718/1‑150 (MQ=255)
                                                                                                                                              ttAACTTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTATCGCCGCAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGCTaa           >  1:368772/1‑150 (MQ=255)
                                                                                                                                              ttAACTTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTATCGCCGCAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGCTaa           >  1:367965/1‑150 (MQ=255)
                                                                                                                                               tAACTTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTATCGCCGCAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGCTAAAt         >  2:521172/1‑151 (MQ=255)
                                                                                                                                                    tttATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTATCGCCGCAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGCTAAATAAcc     >  1:784271/1‑151 (MQ=255)
                                                                                                                                                      tATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTATCGCCGCAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGCTAAATAACCtt   >  2:660296/1‑151 (MQ=255)
                                                                                                                                                      tATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTATCGCCGCAAAACTCCCGCCGCCTATTGTATGAAATGGAGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGCTAAATAACCt    <  1:182895/150‑1 (MQ=255)
                                                                                                                                                       aTTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTATCGCCGCAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGCTAAATAACCtt   >  1:148442/1‑150 (MQ=255)
                                                                                                                                                       aTTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTATCGCCGCAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGCTAAATAACCTTg  <  1:534409/151‑1 (MQ=255)
                                                                                                                                                       |                                                                                                                                                        
TTCACGTAAAGAAAACGGTGAAGTGGTGCGTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTATTTAACTTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTATCGCCGCAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGCTAAATAACCTTG  >  NC_000913/2624366‑2624664

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: