Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,903,692 | 0 | C | A | 43.5% | 129.9 / 68.9 | 92 | intergenic (+318/+1055) | ygcE/queE | putative kinase/7‑carboxy‑7‑deazaguanine synthase; queosine biosynthesis |
Reads supporting (aligned to +/- strand): ref base C (2/50); new base A (40/0); total (42/50) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 4.56e-24 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TTACAAGGAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGTAAAACCACTC‑A‑TT‑AGCCT‑C‑AAAAC‑AAAGACAAACTTCCAG‑ATTTATTACTT‑AACC‑AATGAAT‑AACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTCTTAACG > NC_000913/2903546‑2903841 | ttACAAGGAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTTTTTAAaacaacc > 2:63663/1‑150 (MQ=255) ttACAAGGAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGTAAAACCAc < 1:321846/150‑1 (MQ=255) aCAAGGAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGTAAAACCACTC‑a < 1:32978/151‑1 (MQ=255) cAAGGAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTGTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGTAAAACCACTC‑A‑t < 2:294936/151‑1 (MQ=255) aGGAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGTAAAACCACTC‑A‑TT‑a < 2:676642/151‑1 (MQ=255) aGGAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGTAAAACCACTC‑A‑TT‑a < 1:766724/151‑1 (MQ=255) ggAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATTTAAAAacaccc > 1:739038/1‑144 (MQ=255) atatTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑ATTTTTGAAAcccaacatttca > 1:246571/1‑139 (MQ=255) tatTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑ATATTTTAAAACACTC‑a > 2:248499/1‑143 (MQ=255) atTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATTTAAAAACACCC‑A‑TT‑ATCCt > 1:121626/1‑149 (MQ=255) atTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGTAAAACCACTC‑A‑TT‑AGCCT‑c < 2:451485/150‑1 (MQ=255) aaCTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTTTGTTAAAACACCC‑A‑TT‑AAcccccaa > 1:410124/1‑145 (MQ=255) aaCTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGTAAAACCACTC‑A‑TT‑AGCCT‑C‑aaa < 1:329684/150‑1 (MQ=255) aCTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGTAAAACCACTC‑A‑TT‑AGCCT‑C‑AAAAc < 2:765436/151‑1 (MQ=255) cTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATTTAAAAACACTC‑C‑TT‑AACCT‑C‑aaaa > 1:381304/1‑149 (MQ=255) acacTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGTAAAACCACTC‑A‑TT‑AGCCT‑C‑AAAAC‑aa < 1:267262/150‑1 (MQ=255) acacTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGTAAAACCACTC‑A‑TT‑AGCCT‑C‑AAAAC‑a < 1:752656/149‑1 (MQ=255) acacTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGTAAAAACACTC‑A‑TT‑ATACT‑C‑Aaaaa‑aa > 1:668101/1‑150 (MQ=255) cGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGTAAAACCACTC‑A‑TT‑AGCCT‑C‑AAAAC‑AAAGACa < 1:675617/150‑1 (MQ=255) gATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTTTGTTAAAAAACAC‑A‑AT‑ATGCT‑C‑aaaaa‑aaaaaaaa > 1:74093/1‑145 (MQ=255) tCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTTTTTTAAAACACCC‑A‑AT‑TTCCT‑C‑aaaaa‑aaaaaaaaa > 1:670679/1‑147 (MQ=255) tCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑ATTTTTTAAAACACTC‑A‑TT‑TACCT‑C‑CAAAA‑AAAAACaaaaat > 2:331636/1‑147 (MQ=255) tttAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTTTTTAAAAAAAATC‑A‑TT‑AACCT‑C‑aaaaa‑aaaaaaaaaaattc > 1:763071/1‑145 (MQ=255) tAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTTTTTTAAAACACTC‑A‑TT‑AATCT‑C‑aaaaa‑aaaaaaaa > 2:445573/1‑137 (MQ=255) tAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGTAAAACCACTC‑A‑TT‑AGCCT‑C‑AAAAC‑AAAGACAAACTTCCAg < 2:226089/150‑1 (MQ=255) tAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGTAAAAAAACAC‑A‑TT‑AAACT‑C‑AAAAC‑AAAAACAAAATTcc > 1:594028/1‑148 (MQ=255) aGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGTAAAACCACTC‑A‑TT‑AGCCT‑C‑AAAAC‑AAAGACAAACTTCCAG‑a < 1:630192/150‑1 (MQ=255) gAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATTTAAAAAAAACC‑A‑TT‑ATCCT‑C‑AAAAA‑AAAAAAAAAAATCCAG‑Att > 1:763463/1‑151 (MQ=255) aaTAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTTTTTAAAAACACCC‑C‑TT‑TACCT‑C‑AAAAC‑AAAAAAAAAATTCCAG‑Attt > 1:293952/1‑151 (MQ=255) aaTAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTTTGTTAAAAAAATC‑A‑TT‑ATTCT‑T‑AAAAA‑AAAAAAAAAAATCCAG‑Aatt > 2:432769/1‑151 (MQ=255) aaTAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGTAAAACCACTC‑A‑TT‑AGCCT‑C‑AAAAC‑AAAGACAAACTTCCAG‑Att < 1:659215/150‑1 (MQ=255) tAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTTTGTAAAAACACCC‑A‑TT‑AACCC‑C‑AAAAC‑AAAAAAAAAACTCCAG‑ATttat > 1:733765/1‑151 (MQ=255) aaGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGTAAAACCACTC‑A‑TT‑AGCCT‑C‑AAAAC‑AAAGACAACCTTCCAG‑ATTTATTAc < 2:84219/151‑1 (MQ=255) gACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTCTGTTAAAACAAAC‑C‑TT‑ATCCC‑C‑CAAAC‑AAAAAAAAAATTCCAG‑Atttttt > 1:700140/1‑147 (MQ=255) aaTACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTTTTTTAAAACACTC‑A‑TT‑TATCT‑C‑CAAAA‑AAAAACAAAATTCCCG‑ATTTTTTAttt > 2:274674/1‑148 (MQ=255) aaTACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATTTAAAAAAAATC‑A‑AT‑AACCT‑C‑AAAAA‑AAAAAAAAAATTCCAA‑ATttatttatt > 2:244027/1‑144 (MQ=255) aTACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGTAAAACCACTC‑A‑TT‑AGCCT‑C‑AAAAC‑AAAGACAAACTTCCAG‑ATTTATTACTT‑AAcc < 2:21501/151‑1 (MQ=255) tACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTTTGTTAAAACAAAC‑A‑TT‑ATCCT‑C‑CAAAA‑AAAAAAAAAATTCCAG‑Atttttttatt > 2:223463/1‑142 (MQ=255) aCGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGTAAAACCACTC‑A‑TT‑AGCCT‑C‑AAAAC‑AAAGACAAACTTCCAG‑ATTTATTACTT‑AACC‑aa < 2:612734/151‑1 (MQ=255) cGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGAAAAACCACCC‑A‑TT‑TTTCTCC‑AAAAC‑AAAAAAAAACTTcctaattattaat > 1:215095/1‑132 (MQ=255) gAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTTTTTTAAAACACCC‑C‑TT‑TTCCT‑C‑AAAAC‑AAAAAAAAACATTCAG‑Attttt > 2:391444/1‑136 (MQ=255) cGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGTAAAACCACTC‑A‑TT‑AGCCT‑C‑AAAAC‑AAAGACAAACTTCCAG‑ATTTATTACTT‑AACC‑AATGAAt < 1:648800/150‑1 (MQ=255) cGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGTAAAACCACTC‑A‑TT‑AGCCT‑C‑AAAAC‑AAAGACAAACTTCCAG‑ATTTATTACTT‑AACC‑AATGAAt < 1:415355/150‑1 (MQ=255) cGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGTAAAACCACTC‑A‑TT‑AGCCT‑C‑AAAAC‑AAAGACAAACTTCCAG‑ATTTATTACTT‑AACC‑AATGAAT‑a < 1:193404/151‑1 (MQ=255) cACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGTAAAACCACTC‑A‑TT‑AGCCT‑C‑AAAAC‑AAAGACAAACTTCCAG‑ATTTATTACTT‑AACC‑AATGAAT‑aa < 2:446730/150‑1 (MQ=255) cTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTTTTTAAAAACCCCC‑C‑TT‑ACACT‑CAAAAAA‑AAAAAAAAACTACCCG‑ATTTTTTTTTT‑AACC‑‑ATAAAT‑AACa > 1:751701/1‑150 (MQ=255) ttATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTTTTTAAAAACAACC‑A‑TT‑TACCT‑C‑AAAAA‑AAAAAAAAAAATCCAG‑AATTTTTTTTT‑TACC‑AAAAAAT‑aaaa > 1:550928/1‑146 (MQ=255) ttATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGTAAAACCACTC‑A‑TT‑AGCCT‑C‑AAAAC‑AAAGACAAACTTCCAG‑ATTTATTACTT‑AACC‑AATGAAT‑AACAAg < 1:517067/150‑1 (MQ=255) ttATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGTAAAACCACTC‑A‑TT‑AGCCT‑C‑AAAAC‑AAAGACAAACTTCCAG‑ATTTATTACTT‑AACC‑AATGAAT‑AACAAg < 1:726599/150‑1 (MQ=255) aTCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGTAAAACCACTC‑A‑TT‑AGCCT‑C‑AAAAC‑AAAGACAAACTTCCAG‑ATTTATTACTT‑AACC‑AATGAAT‑AACAAGCt < 2:477058/150‑1 (MQ=255) tCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATTTATAAAAACTA‑A‑TT‑ACCCT‑C‑AAAAA‑AAAAACAAAAAACCTA‑ATTTATTAATT‑TACC‑AAAGAAT‑AAAAAgat > 1:307827/1‑147 (MQ=255) cAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGTAAAACCACTC‑A‑TT‑AGCCT‑C‑AAAAC‑AAAGACAAACTTCCAG‑ATTTATTACTT‑AACC‑AATGAAT‑AACAAGCTTaa < 2:762920/151‑1 (MQ=255) aTTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTTAAAACCCCCC‑A‑TT‑‑CCCC‑C‑AAAAA‑AAAAACAAAAATCCCGCTTTTTTTATTT‑AACC‑‑ATGAAT‑AACAAACTTtaa > 2:518615/1‑150 (MQ=255) tttCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGTAAAACCACTC‑A‑TT‑AGCCT‑C‑AAAAC‑AAAGACAAACTTCCAG‑ATTTATTACTT‑AACC‑AATGAAT‑AACAAGCTTaaaaa < 2:420067/150‑1 (MQ=255) tttCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGTAAAACCACTC‑A‑TT‑AGCCT‑C‑AAAAC‑AAAGACAAACTTCCAG‑ATTTATTACTT‑AACC‑AATGAAT‑AACAAGCTTaaaaa < 2:794161/150‑1 (MQ=255) cTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGTAAAACCACTC‑A‑TT‑AGCCT‑C‑AAAAC‑AAAGACAAACTTCCAG‑ATTTATTACTT‑AACC‑AATGAAT‑AACAAGCTTAAAAAAtata < 1:313726/150‑1 (MQ=255) tAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGTAAAACCACTC‑A‑TT‑AGCCT‑C‑AAAAC‑AAAGACAAACTTCCAG‑ATTTATTACTT‑AACC‑AATGAAT‑AACAAGCTTAAAAAATATAc < 2:67347/149‑1 (MQ=255) aaaGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGTAAAACCACTC‑A‑TT‑AGCCT‑C‑AAAAC‑AAAGACAAACTTCCAG‑ATTTATTACTT‑AACC‑AATGAAT‑AACAAGCTTAAAAAATATACTg < 2:283442/150‑1 (MQ=255) aaGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTTTTTAAAAAAACTA‑C‑TT‑TACCT‑C‑CAAACAAAAAAAAAAATCCCAA‑ATTTTTTATTT‑AA‑C‑AAAGAAT‑AAAAAAATTAAAAAAaataatttt > 2:599807/1‑146 (MQ=255) aaGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGTAAAACCACTC‑A‑TT‑AGCCT‑C‑AAAAC‑AAAGACAAACTTCCAG‑ATTTATTACTT‑AACC‑AATGAAT‑AACAAGCTTAAAAAATATACTGtt < 1:657090/151‑1 (MQ=255) gTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTTTTTTAAAACACCC‑A‑TT‑AGCCT‑C‑AAAAA‑AAAAAAAAAAATTCCG‑ATTTTTTATTT‑AAAC‑AATAAAA‑AAAAAAATTAAAAAAAATAATGtttt > 1:555626/1‑149 (MQ=255) aTGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGTAAAACCACTC‑A‑TT‑AGCCT‑C‑AAAAC‑AAAGACAAACTTCCAG‑ATTTATTACTT‑AACC‑AATGAAT‑AACAAGCTTAAAAAATATACTGTTAtt < 2:746416/150‑1 (MQ=255) gAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATTTAAAAACACTC‑A‑TT‑ATCCC‑C‑CAAAA‑AAAAAAAAAACTCCAG‑ATTTATTTTTT‑AAAC‑AACTAAT‑AACAAACTTtaaaaaaaaattatt > 1:415588/1‑136 (MQ=255) gAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGTAAAACCACTC‑A‑TT‑AGCCT‑C‑AAAAC‑AAAGACAAACTTCCAG‑ATTTATTACTT‑AACC‑AATGAAT‑AACAAGCTTAAAAAATATACTGTTATTCt < 2:78749/150‑1 (MQ=255) aaCTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGTAAAACCACTC‑A‑TT‑AGCCT‑C‑AAAAC‑AAAGACAAACTTCCAG‑ATTTATTACTT‑AACC‑AATGAAT‑AACAAGCTTAAAAAATATACTGTTATTCTaa < 1:231171/151‑1 (MQ=255) aCTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTTTGTAAAAACACTC‑A‑TT‑AACCT‑C‑AAAAC‑AAAAAAAAAAATTCAG‑ATTTTTTTCTT‑AACC‑AAAAAAT‑AAAAAACTTAAAAAAAATACTGTTAtttt > 1:259106/1‑146 (MQ=255) gATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGTAAAACCACTC‑A‑TT‑AGCCT‑C‑AAAAC‑AAAGACAAACTTCCAG‑ATTTATTACTT‑AACC‑AATGAAT‑AACAAGCTTAAAAAATATACTGTTATTCTAAAGaaa < 2:213942/151‑1 (MQ=255) cTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTTTTTTAAAAAACAC‑C‑TT‑TGCCC‑C‑AAAAA‑AAAAAAAAAAATCCAC‑AATTTTTTTTT‑AAAC‑AAAAAAA‑AAAAAACATAAAAAAAAAAATATTTTTTTTAaaaaaaaa > 2:526370/1‑149 (MQ=255) tttATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑GTATTTAAAAACCCTC‑ATTT‑AGCCT‑‑‑AAAAA‑AAAAAAAAAAATCCTG‑AATTTTTAATT‑AAAC‑AAAGAATAAAAAAGATTAAAAAA‑ATAAACTTTTTCTTAAGaaaaaa > 1:99375/1‑148 (MQ=255) tATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGTAAAACCACTC‑A‑TT‑AGCCT‑C‑AAAAC‑AAAGACAAACTTCCAG‑ATTTATTACTT‑AACC‑AATGAAT‑AACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTaa < 1:205638/150‑1 (MQ=255) aTTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTTTTTTAAAACACCC‑C‑TT‑ATCCT‑C‑AAAAA‑AAAAAAAAAATTTCCC‑ATTTTTTTTTT‑TAAC‑AATGAAA‑AAAAAAATTAAAAAAAATAATGTTTTTTTTAAAAAAAAAATaa > 1:98093/1‑149 (MQ=255) ttCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTTTTTTAAAACACTCAA‑TT‑AACCC‑C‑AAAAA‑AAGAAAAAAATTCTCG‑ATTTTTTACTT‑AACA‑AAT‑AAG‑AAAAAACTTAAAAAAAATATTGTTTTTTTTAaaaaaaaa > 2:342017/1‑144 (MQ=255) ttCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTTTGTAAAAACACCC‑A‑CT‑TAACT‑C‑AAAAA‑AAAAAAAAACTTCCAC‑ATTTATTACTTAAACA‑AACAAAA‑AAAAAACTTAAAAAA‑ATAATGTTTTTTTTAAAAAAAAAATaaaa > 1:792074/1‑148 (MQ=255) ttCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGTAAAAACACCC‑A‑TT‑AACCT‑C‑AAAAA‑AAAAAAAAAATTTCCA‑ATTTATTTATT‑AAAC‑AAAGAAT‑AAAAAACTTAAAAAAAAAAATTTTTTTTTAAAGAAAAAAATaa > 1:273424/1‑148 (MQ=255) tCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGTAAAACCACTC‑A‑TT‑AGCCT‑C‑AAAAC‑AAAGACAAACTTCCAG‑ATTTATTACTT‑AACC‑AATGAAT‑AACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAg < 1:305437/150‑1 (MQ=255) cATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTTTTTTAAAAAACAC‑A‑TT‑TAACC‑C‑AAAAA‑AAAAAAAAAAATTCAG‑ATTTTTTTTTT‑TAAC‑AAATAAA‑AAAAAAATTAAAAAAAATAATATTATTTTTAAAAAAAAAATaaaaa > 2:247972/1‑146 (MQ=255) aaGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGTAAAACCACTC‑A‑TT‑AGCCT‑C‑AAAAC‑AAAGACAAACTTCCAG‑ATTTATTACTT‑AACC‑AATGAAT‑AACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCAtt < 2:435452/151‑1 (MQ=255) aGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGTAAAACCACTC‑A‑TT‑AGCCT‑C‑AAAAC‑AAAGACAAACTTCCAG‑ATTTATTACTT‑AACC‑AATGAAT‑AACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTa < 1:518659/151‑1 (MQ=255) aCTATTCATCAACAGCGTATAGAGGCGG‑TTATGTAAAACCACTC‑A‑TT‑AGCCT‑C‑AAAAC‑AAAGACAAACTTCCAG‑ATTTATTACTT‑AACC‑AATGAAT‑AACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAAtt < 2:670630/151‑1 (MQ=255) aTTCATCAACAGCGTATAGAGGCGG‑ATTTGTAAAAACACTC‑A‑TT‑TGCCT‑C‑AAAAA‑CAAAAAAAACATCCAG‑ATTTATTTTTT‑AACA‑AAAGAAA‑AAAAAAAATAAAAAAAATAATTTTTTTTTAAAAAAAAAAATAAAAAGCATTAAt > 2:587991/1‑147 (MQ=255) ttCATCAACAGCGTATAGAGGCGG‑TTTTTTAAAAACACCC‑C‑TT‑TACCT‑C‑CAAAA‑AAAAAAAAACATTCCG‑ATTTTTTTCTT‑TAAC‑AAAGAAA‑AAAAAAATTTAAAAAAATACTGTTTTTTTTAAAAAAAAAATAAAAAGCATTAATTTaa > 1:754884/1‑150 (MQ=255) ttCATCAACAGCGTATAGAGGCGG‑TTTTGTTAAAACACCC‑A‑CT‑TTACC‑C‑AAAAC‑AAAAAAAAAAAATCAG‑GATTATTACTT‑AACC‑AAAAAAA‑AAAAAAAATAAAAAAAATAAATGTATTTTTAAGAAAAAAATAAAAAGGATTAATTTaa > 2:282519/1‑150 (MQ=255) tcatcaACAGCGTATAGAGGCGG‑TTTTGTTAAAAAACAC‑A‑TT‑AACCC‑C‑CAAAA‑AAAAACAAAAATCCCG‑ATTTTTTTTTT‑TAAC‑AAAAAAA‑AAAAAAATTAAAAAAAATAATGTTTTTTTTAAAAAAAAAATAAAAAGCATTAAtt > 1:14425/1‑146 (MQ=255) tcatcaACAGCGTATAGAGGCGG‑TTATGTAAAACCACTC‑A‑TT‑AGCCT‑C‑AAAAC‑AAAGACAAACTTCCAG‑ATTTATTACTT‑AACC‑AATGAAT‑AACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAat < 1:461959/150‑1 (MQ=255) catcaACAGCGTATAGAGGCGG‑TTTTTTAAAAACACTC‑C‑TTAACCCC‑C‑AAAAA‑AAAAAAAAACTT‑CAG‑ATTTTTTTATT‑TAAC‑AAAGAAA‑AAAAAAATTAAAAAAAAAAATATTTTTTTTAAAAAAAAAATAAAAAGCAATAATTTaaaa > 2:403646/1‑148 (MQ=255) caACAGCGTATAGAGGCGG‑TTATGTAAAACCACTC‑A‑TT‑AGCCT‑C‑AAAAC‑AAAGACAAACTTCCAG‑ATTTATTACTT‑AACC‑AATGAAT‑AACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTa < 2:187921/150‑1 (MQ=255) aGCGTATAGAGGCGG‑TTATGTAAAACCACTC‑A‑TT‑AGCCT‑C‑AAAAC‑AAAGACAAACTTCCAG‑ATTTATTACTT‑AACC‑AATGAAT‑AACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTaaaaaa < 1:328721/151‑1 (MQ=255) aGCGTATAGAGGCGG‑TTATGTAAAAACACAC‑C‑TT‑AACCT‑C‑CAAAC‑AAAAACAAACATCCAA‑AATTTTTTATT‑AACCAAAAAAAA‑AAAAAAATTAAAAAAAAAATTTTTTTTTTAAA‑AAAAAAATAAAAAGCATTAAATTAATATTtaaaa > 2:548868/1‑150 (MQ=255) ataGAGGCGG‑TTATGTAAAACCACTC‑A‑TT‑AGCCT‑C‑AAAAC‑AAAGACAAACTTCCAG‑ATTTATTACTT‑AACC‑AATGAAT‑AACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAaataat < 2:40462/151‑1 (MQ=255) g‑TTATGTAAAACCACTC‑A‑TT‑AGCCT‑C‑AAAAC‑AAAGACAAACTTCCAG‑ATTTATTACTT‑AACC‑AATGAAT‑AACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTaaaa < 2:556613/151‑1 (MQ=255) tATGTAAAACCACTC‑A‑TT‑AGCCT‑C‑AAAGC‑AAAGACAAACTTCCAG‑ATTTATTACTT‑AACC‑AATGAAT‑AACAAGCTTAAAAAATATACAGTTATTCTAAAGAAAAAATTAACTGGCAGTAATTCAATATTAAAAAAATAATAGATTAAGAt < 2:508568/150‑1 (MQ=255) aTGTAAAACCACTC‑A‑TT‑AGCCT‑C‑AAAAC‑AAAGACAAACTTCCAG‑ATTTATTACTT‑AACC‑AATGAAT‑AACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATGATAGATTAAAAtt < 1:129422/150‑1 (MQ=255) aTGTAAAACCACTC‑A‑TT‑AGCCT‑C‑AAAAC‑AAAGACAAACTTCCAG‑ATTTATTACTT‑AACC‑AATGAAT‑AACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAAttt < 1:648875/151‑1 (MQ=255) tGTAAAACCACTC‑A‑TT‑AGCCT‑C‑AAAAC‑AAAGACAAACTTCCAG‑ATTTATTACTT‑AACC‑AATGAAT‑AACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAAttt < 2:778511/150‑1 (MQ=255) aaCCACTC‑A‑TT‑AGCCT‑C‑AAAAC‑AAAGACAAACTTCCAG‑ATTTATTACTT‑AACC‑AATGAAT‑AACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTCTTAAc < 2:655821/151‑1 (MQ=255) ccACTC‑A‑TT‑AGCCT‑C‑AAAAC‑AAAGACAAACTTCCAG‑ATTTATTACTT‑AACC‑AATGAAT‑AACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTCTTAACg > 1:622425/1‑150 (MQ=255) | TTACAAGGAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGTAAAACCACTC‑A‑TT‑AGCCT‑C‑AAAAC‑AAAGACAAACTTCCAG‑ATTTATTACTT‑AACC‑AATGAAT‑AACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTCTTAACG > NC_000913/2903546‑2903841 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |