Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,903,6920CA43.5% 129.9 / 68.9 92intergenic (+318/+1055)ygcE/queEputative kinase/7‑carboxy‑7‑deazaguanine synthase; queosine biosynthesis
Reads supporting (aligned to +/- strand):  ref base C (2/50);  new base A (40/0);  total (42/50)
Fisher's exact test for biased strand distribution p-value = 4.56e-24
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTACAAGGAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTCTTAACG  >  NC_000913/2903546‑2903841
                                                                                                                                                   |                                                                                                                                                               
ttACAAGGAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTAAaacaacc                                                                                                                                                             >  2:63663/1‑150 (MQ=255)
ttACAAGGAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCAc                                                                                                                                                              <  1:321846/150‑1 (MQ=255)
  aCAAGGAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCa                                                                                                                                                          <  1:32978/151‑1 (MQ=255)
   cAAGGAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTGTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCAt                                                                                                                                                        <  2:294936/151‑1 (MQ=255)
     aGGAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTa                                                                                                                                                     <  2:676642/151‑1 (MQ=255)
     aGGAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTa                                                                                                                                                     <  1:766724/151‑1 (MQ=255)
      ggAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATTTAAAAacaccc                                                                                                                                                            >  1:739038/1‑144 (MQ=255)
         atatTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGATTTTTGAAAcccaacatttca                                                                                                                                                      >  1:246571/1‑139 (MQ=255)
          tatTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGATATTTTAAAACACTCa                                                                                                                                                          >  2:248499/1‑143 (MQ=255)
           atTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATTTAAAAACACCCATTATCCt                                                                                                                                                 >  1:121626/1‑149 (MQ=255)
           atTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTc                                                                                                                                               <  2:451485/150‑1 (MQ=255)
              aaCTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTGTTAAAACACCCATTAAcccccaa                                                                                                                                             >  1:410124/1‑145 (MQ=255)
              aaCTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCaaa                                                                                                                                           <  1:329684/150‑1 (MQ=255)
               aCTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAAc                                                                                                                                         <  2:765436/151‑1 (MQ=255)
                cTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATTTAAAAACACTCCTTAACCTCaaaa                                                                                                                                          >  1:381304/1‑149 (MQ=255)
                  acacTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACaa                                                                                                                                      <  1:267262/150‑1 (MQ=255)
                  acacTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACa                                                                                                                                       <  1:752656/149‑1 (MQ=255)
                  acacTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAAACACTCATTATACTCAaaaaaa                                                                                                                                      >  1:668101/1‑150 (MQ=255)
                       cGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACa                                                                                                                                 <  1:675617/150‑1 (MQ=255)
                        gATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTGTTAAAAAACACAATATGCTCaaaaaaaaaaaaa                                                                                                                                >  1:74093/1‑145 (MQ=255)
                            tCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTTAAAACACCCAATTTCCTCaaaaaaaaaaaaaa                                                                                                                               >  1:670679/1‑147 (MQ=255)
                            tCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGATTTTTTAAAACACTCATTTACCTCCAAAAAAAAACaaaaat                                                                                                                            >  2:331636/1‑147 (MQ=255)
                              tttAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTAAAAAAAATCATTAACCTCaaaaaaaaaaaaaaaattc                                                                                                                          >  1:763071/1‑145 (MQ=255)
                                tAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTTAAAACACTCATTAATCTCaaaaaaaaaaaaa                                                                                                                                >  2:445573/1‑137 (MQ=255)
                                tAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAg                                                                                                                        <  2:226089/150‑1 (MQ=255)
                                tAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAAAAACACATTAAACTCAAAACAAAAACAAAATTcc                                                                                                                          >  1:594028/1‑148 (MQ=255)
                                 aGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGa                                                                                                                      <  1:630192/150‑1 (MQ=255)
                                  gAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATTTAAAAAAAACCATTATCCTCAAAAAAAAAAAAAAAATCCAGAtt                                                                                                                    >  1:763463/1‑151 (MQ=255)
                                   aaTAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTAAAAACACCCCTTTACCTCAAAACAAAAAAAAAATTCCAGAttt                                                                                                                   >  1:293952/1‑151 (MQ=255)
                                   aaTAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTGTTAAAAAAATCATTATTCTTAAAAAAAAAAAAAAAATCCAGAatt                                                                                                                   >  2:432769/1‑151 (MQ=255)
                                   aaTAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGAtt                                                                                                                    <  1:659215/150‑1 (MQ=255)
                                     tAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTGTAAAAACACCCATTAACCCCAAAACAAAAAAAAAACTCCAGATttat                                                                                                                 >  1:733765/1‑151 (MQ=255)
                                        aaGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAACCTTCCAGATTTATTAc                                                                                                              <  2:84219/151‑1 (MQ=255)
                                          gACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTCTGTTAAAACAAACCTTATCCCCCAAACAAAAAAAAAATTCCAGAtttttt                                                                                                                >  1:700140/1‑147 (MQ=255)
                                             aaTACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTTAAAACACTCATTTATCTCCAAAAAAAAACAAAATTCCCGATTTTTTAttt                                                                                                            >  2:274674/1‑148 (MQ=255)
                                             aaTACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATTTAAAAAAAATCAATAACCTCAAAAAAAAAAAAAAATTCCAAATttatttatt                                                                                                            >  2:244027/1‑144 (MQ=255)
                                              aTACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAAcc                                                                                                       <  2:21501/151‑1 (MQ=255)
                                               tACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTGTTAAAACAAACATTATCCTCCAAAAAAAAAAAAAATTCCAGAtttttttatt                                                                                                            >  2:223463/1‑142 (MQ=255)
                                                aCGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCaa                                                                                                    <  2:612734/151‑1 (MQ=255)
                                                 cGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGAAAAACCACCCATTTTTCTCCAAAACAAAAAAAAACTTcctaattattaat                                                                                                               >  1:215095/1‑132 (MQ=255)
                                                  gAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTTAAAACACCCCTTTTCCTCAAAACAAAAAAAAACATTCAGAttttt                                                                                                                 >  2:391444/1‑136 (MQ=255)
                                                      cGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAAt                                                                                               <  1:648800/150‑1 (MQ=255)
                                                      cGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAAt                                                                                               <  1:415355/150‑1 (MQ=255)
                                                      cGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATa                                                                                             <  1:193404/151‑1 (MQ=255)
                                                        cACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATaa                                                                                            <  2:446730/150‑1 (MQ=255)
                                                          cTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTAAAAACCCCCCTTACACTCAAAAAAAAAAAAAAACTACCCGATTTTTTTTTTAACCATAAATAACa                                                                                          >  1:751701/1‑150 (MQ=255)
                                                            ttATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTAAAAACAACCATTTACCTCAAAAAAAAAAAAAAAATCCAGAATTTTTTTTTTACCAAAAAATaaaa                                                                                          >  1:550928/1‑146 (MQ=255)
                                                            ttATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAg                                                                                        <  1:517067/150‑1 (MQ=255)
                                                            ttATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAg                                                                                        <  1:726599/150‑1 (MQ=255)
                                                              aTCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCt                                                                                      <  2:477058/150‑1 (MQ=255)
                                                               tCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATTTATAAAAACTAATTACCCTCAAAAAAAAAACAAAAAACCTAATTTATTAATTTACCAAAGAATAAAAAgat                                                                                      >  1:307827/1‑147 (MQ=255)
                                                                cAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTaa                                                                                   <  2:762920/151‑1 (MQ=255)
                                                                  aTTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTTAAAACCCCCCATTCCCCCAAAAAAAAAACAAAAATCCCGCTTTTTTTATTTAACCATGAATAACAAACTTtaa                                                                                  >  2:518615/1‑150 (MQ=255)
                                                                    tttCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTaaaaa                                                                                <  2:420067/150‑1 (MQ=255)
                                                                    tttCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTaaaaa                                                                                <  2:794161/150‑1 (MQ=255)
                                                                         cTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAAtata                                                                           <  1:313726/150‑1 (MQ=255)
                                                                           tAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATAc                                                                          <  2:67347/149‑1 (MQ=255)
                                                                            aaaGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTg                                                                        <  2:283442/150‑1 (MQ=255)
                                                                             aaGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTAAAAAAACTACTTTACCTCCAAACAAAAAAAAAAATCCCAAATTTTTTATTTAACAAAGAATAAAAAAATTAAAAAAaataatttt                                                                      >  2:599807/1‑146 (MQ=255)
                                                                             aaGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGtt                                                                      <  1:657090/151‑1 (MQ=255)
                                                                               gTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTTAAAACACCCATTAGCCTCAAAAAAAAAAAAAAAATTCCGATTTTTTATTTAAACAATAAAAAAAAAAATTAAAAAAAATAATGtttt                                                                    >  1:555626/1‑149 (MQ=255)
                                                                                 aTGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTAtt                                                                   <  2:746416/150‑1 (MQ=255)
                                                                                   gAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATTTAAAAACACTCATTATCCCCCAAAAAAAAAAAAAACTCCAGATTTATTTTTTAAACAACTAATAACAAACTTtaaaaaaaaattatt                                                                      >  1:415588/1‑136 (MQ=255)
                                                                                   gAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCt                                                                 <  2:78749/150‑1 (MQ=255)
                                                                                    aaCTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTaa                                                               <  1:231171/151‑1 (MQ=255)
                                                                                     aCTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTGTAAAAACACTCATTAACCTCAAAACAAAAAAAAAAATTCAGATTTTTTTCTTAACCAAAAAATAAAAAACTTAAAAAAAATACTGTTAtttt                                                                 >  1:259106/1‑146 (MQ=255)
                                                                                         gATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGaaa                                                          <  2:213942/151‑1 (MQ=255)
                                                                                              cTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTTAAAAAACACCTTTGCCCCAAAAAAAAAAAAAAAATCCACAATTTTTTTTTAAACAAAAAAAAAAAAACATAAAAAAAAAAATATTTTTTTTAaaaaaaaa                                                       >  2:526370/1‑149 (MQ=255)
                                                                                               tttATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGGTATTTAAAAACCCTCATTTAGCCTAAAAAAAAAAAAAAAATCCTGAATTTTTAATTAAACAAAGAATAAAAAAGATTAAAAAAATAAACTTTTTCTTAAGaaaaaa                                                       >  1:99375/1‑148 (MQ=255)
                                                                                                 tATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTaa                                                   <  1:205638/150‑1 (MQ=255)
                                                                                                  aTTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTTAAAACACCCCTTATCCTCAAAAAAAAAAAAAAATTTCCCATTTTTTTTTTTAACAATGAAAAAAAAAATTAAAAAAAATAATGTTTTTTTTAAAAAAAAAATaa                                                   >  1:98093/1‑149 (MQ=255)
                                                                                                   ttCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTTAAAACACTCAATTAACCCCAAAAAAAGAAAAAAATTCTCGATTTTTTACTTAACAAATAAGAAAAAACTTAAAAAAAATATTGTTTTTTTTAaaaaaaaa                                                       >  2:342017/1‑144 (MQ=255)
                                                                                                   ttCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTGTAAAAACACCCACTTAACTCAAAAAAAAAAAAAACTTCCACATTTATTACTTAAACAAACAAAAAAAAAACTTAAAAAAATAATGTTTTTTTTAAAAAAAAAATaaaa                                                 >  1:792074/1‑148 (MQ=255)
                                                                                                   ttCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAAACACCCATTAACCTCAAAAAAAAAAAAAAATTTCCAATTTATTTATTAAACAAAGAATAAAAAACTTAAAAAAAAAAATTTTTTTTTAAAGAAAAAAATaa                                                   >  1:273424/1‑148 (MQ=255)
                                                                                                    tCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAg                                                <  1:305437/150‑1 (MQ=255)
                                                                                                     cATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTTAAAAAACACATTTAACCCAAAAAAAAAAAAAAAATTCAGATTTTTTTTTTTAACAAATAAAAAAAAAATTAAAAAAAATAATATTATTTTTAAAAAAAAAATaaaaa                                                >  2:247972/1‑146 (MQ=255)
                                                                                                        aaGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCAtt                                           <  2:435452/151‑1 (MQ=255)
                                                                                                         aGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTa                                          <  1:518659/151‑1 (MQ=255)
                                                                                                            aCTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAAtt                                       <  2:670630/151‑1 (MQ=255)
                                                                                                               aTTCATCAACAGCGTATAGAGGCGGATTTGTAAAAACACTCATTTGCCTCAAAAACAAAAAAAACATCCAGATTTATTTTTTAACAAAAGAAAAAAAAAAATAAAAAAAATAATTTTTTTTTAAAAAAAAAAATAAAAAGCATTAAt                                        >  2:587991/1‑147 (MQ=255)
                                                                                                                ttCATCAACAGCGTATAGAGGCGGTTTTTTAAAAACACCCCTTTACCTCCAAAAAAAAAAAAACATTCCGATTTTTTTCTTTAACAAAGAAAAAAAAAATTTAAAAAAATACTGTTTTTTTTAAAAAAAAAATAAAAAGCATTAATTTaa                                    >  1:754884/1‑150 (MQ=255)
                                                                                                                ttCATCAACAGCGTATAGAGGCGGTTTTGTTAAAACACCCACTTTACCCAAAACAAAAAAAAAAAATCAGGATTATTACTTAACCAAAAAAAAAAAAAAATAAAAAAAATAAATGTATTTTTAAGAAAAAAATAAAAAGGATTAATTTaa                                    >  2:282519/1‑150 (MQ=255)
                                                                                                                 tcatcaACAGCGTATAGAGGCGGTTTTGTTAAAAAACACATTAACCCCCAAAAAAAAACAAAAATCCCGATTTTTTTTTTTAACAAAAAAAAAAAAAATTAAAAAAAATAATGTTTTTTTTAAAAAAAAAATAAAAAGCATTAAtt                                       >  1:14425/1‑146 (MQ=255)
                                                                                                                 tcatcaACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAat                                   <  1:461959/150‑1 (MQ=255)
                                                                                                                  catcaACAGCGTATAGAGGCGGTTTTTTAAAAACACTCCTTAACCCCCAAAAAAAAAAAAAACTTCAGATTTTTTTATTTAACAAAGAAAAAAAAAATTAAAAAAAAAAATATTTTTTTTAAAAAAAAAATAAAAAGCAATAATTTaaaa                                  >  2:403646/1‑148 (MQ=255)
                                                                                                                     caACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTa                               <  2:187921/150‑1 (MQ=255)
                                                                                                                         aGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTaaaaaa                          <  1:328721/151‑1 (MQ=255)
                                                                                                                         aGCGTATAGAGGCGGTTATGTAAAAACACACCTTAACCTCCAAACAAAAACAAACATCCAAAATTTTTTATTAACCAAAAAAAAAAAAAAATTAAAAAAAAAATTTTTTTTTTAAAAAAAAAATAAAAAGCATTAAATTAATATTtaaaa                           >  2:548868/1‑150 (MQ=255)
                                                                                                                              ataGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAaataat                     <  2:40462/151‑1 (MQ=255)
                                                                                                                                       gTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTaaaa            <  2:556613/151‑1 (MQ=255)
                                                                                                                                          tATGTAAAACCACTCATTAGCCTCAAAGCAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACAGTTATTCTAAAGAAAAAATTAACTGGCAGTAATTCAATATTAAAAAAATAATAGATTAAGAt           <  2:508568/150‑1 (MQ=255)
                                                                                                                                           aTGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATGATAGATTAAAAtt          <  1:129422/150‑1 (MQ=255)
                                                                                                                                           aTGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAAttt         <  1:648875/151‑1 (MQ=255)
                                                                                                                                            tGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAAttt         <  2:778511/150‑1 (MQ=255)
                                                                                                                                                 aaCCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTCTTAAc   <  2:655821/151‑1 (MQ=255)
                                                                                                                                                   ccACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTCTTAACg  >  1:622425/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                               
TTACAAGGAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTCTTAACG  >  NC_000913/2903546‑2903841

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: