| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,478,324 | 0 | T | G | 44.4% | 74.1 / 11.5 | 45 | G33G (GGT→GGG) | ynbB | putative CDP‑diglyceride synthase |
| Reads supporting (aligned to +/- strand): ref base T (3/22); new base G (20/0); total (23/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.32e-10 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGACTGCGATTAATCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCG‑TACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTACA > NC_000913/1478174‑1478470 | ggACTGCGATTAATCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGAGAAAAGGGGGt < 1:70543/151‑1 (MQ=255) aTTAATCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTAACGGGGAGAAAAGGGGGGGAgggt > 1:561995/1‑147 (MQ=255) cGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGAGAAAAGGGGGTGAGGTTACATTACg < 2:88436/151‑1 (MQ=255) tGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGAGAAAAGGGGGTGAGGTTACATTACGTa < 2:211304/150‑1 (MQ=255) cGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGAGAAAAGGGGGTGAGGTTACATTACGTATTc < 1:220891/150‑1 (MQ=255) ggAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTTCCGGAGAGAAAAGGGGGGGAGGGTATATTACGTAtt > 1:712918/1‑148 (MQ=255) gAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTTTCGGAGGGAAAAAGGGGGGGGGGTATATTACTTa > 2:33870/1‑145 (MQ=255) gAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCG‑Ta < 2:190916/151‑1 (MQ=255) gtgtGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCG‑TAcc < 1:790357/151‑1 (MQ=255) gtgCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCG‑TACCtg < 1:369304/151‑1 (MQ=255) cttcttATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTAACGGGGAGAAAAGGGGGGGAGGGTACAATACGTATTCG‑TAACttggg > 2:638936/1‑147 (MQ=255) ttcttATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTTTCGGGGAGGAAAGGGGGGGGGGGTACATTACGCATTCG‑TTACtggggg > 1:600323/1‑151 (MQ=255) aTGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTTCCCGGGAGAAAAGGGGGGGAGGGTACATTACGTATTCG‑TACCTGGTgggg > 2:557279/1‑148 (MQ=255) gcTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCG‑TACCTGGTGGGGCATCGTCAt < 2:210253/151‑1 (MQ=255) aaTATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTAACGGAGAGAAAAGGGGGGGGGGGTACATTATGTATTCG‑TACCTGGTGGGGGATCGTCAtttttt > 2:59899/1‑151 (MQ=255) tataATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGGGGGAAAAAGGGGGGGGGGTATATTTCGTATTCT‑TTCCTGGTGGGGGGTCGTCAtttttttt > 2:302580/1‑151 (MQ=255) tataATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGAGAAAAAGGGGGGGGGGTTCATTACGTATTCG‑TTCCCGGGGGGGCAACGTCCTTTGtttt > 1:295913/1‑151 (MQ=255) aTGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCG‑TACCTGGTGGGGCATCGTCATTTGTTTTTCaa < 2:637015/151‑1 (MQ=255) gCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGGGAGAAAAAGGGGGGGGGGTTCATTACGTATTCG‑TTACTTGGGGGGGATCGTCATtttttttttca > 1:212119/1‑146 (MQ=255) cTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGGGGGAAAAGGGGGGGGGGGTTTATTTCGGTTTTG‑TACCTGGTGGGGGGTCGTCCTTTTTTTTTTAAttg > 1:528178/1‑149 (MQ=255) ggAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCG‑TACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGt < 1:140942/150‑1 (MQ=255) aaTCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTATCAGCGGGGAAAAGGGGGGGGGGTATATTTCGTCTTTGTTCCCGGGTGGGGGACGTTCATtttttttttatcgtggttt > 2:400085/1‑139 (MQ=255) aaTCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGAGAAAAAGGGGGGAGGGTACATTACGTATTTG‑TACCTGGGGGGGGATCGTCCTTTTTTTTTTAATGGGGAtttt > 1:679208/1‑150 (MQ=255) ctctGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTTTCGGGGAGAAAAGGGGGGGGGGGTTAATTTCGGATTTG‑TTGCTGGGGGGGCGTCGTCATTTTTTTTTTTATTGTGGttt > 2:343130/1‑147 (MQ=255) ctctGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTAACGGAGGGAAAAGGGGGGGGGGGTACATTACGTATTTG‑TACCTGGGGGGGGATCGTCATTTGTTTTTTAATTGTGATTTCAgg > 2:46649/1‑151 (MQ=255) ctGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCG‑TACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCa < 1:755031/147‑1 (MQ=255) ctGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCG‑TACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAg < 2:671358/148‑1 (MQ=255) ggCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCG‑TACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGTTc < 2:148910/149‑1 (MQ=255) cAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCG‑TACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACg < 1:735089/151‑1 (MQ=255) aaCACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACTGGGGAGAAAAAAGGGGGGGGGTACATTTCTAATTCG‑TACCTGGTGGGGGGTAGTCATTTGTTTTTTCATggg > 2:387149/1‑134 (MQ=255) tttCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGGGAGAAAAGGGGGGGAGGGTACATTACGTATTTG‑TACCTGGTGGGGGATCGGCATTTGTTTTTCAATGGGGATTTTAGGTCCACGCTGGAt > 1:368922/1‑150 (MQ=255) aaTTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCG‑TACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATgacgttgacgtt < 2:634560/150‑1 (MQ=255) tttAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGGGAGAAAAGGGGGGGGGGGTACATTACGTATTCG‑TACCTGGGGGGGGATCGTCATTTGTTTTTTAATTGGGGTTTTAGGTCCACGCTGGGTGACGTTGACGtttttt > 1:738358/1‑151 (MQ=255) ttAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCCGGGAGAAAAGGGGGGGAGGGTACATTACGTATTCG‑TACCTGGTGGGGCATCGTCATTTTTTTTTTAATGGTGGTTTCAGGGCCACGCCGGGTGACGTTGACGtttttt > 1:447263/1‑150 (MQ=255) tAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGGGGGAAAAGGGGGGGAGGGTACATTTCGTATTCG‑TACCTGGTGGGGGATCGTCATTTGTTTTTTAATGGGGGTTTTTGGTCCACCCTGGATGGCGTTTACGtttttt > 2:607063/1‑149 (MQ=255) aCGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCG‑TACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTg < 2:113701/150‑1 (MQ=255) gATAAACCGTTTTCTGCTATGGCGGTTACCGGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCG‑TACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAg < 2:512261/150‑1 (MQ=255) ttCTGCTATGGCGGTTACCGGAGAGAAAAGGGTGTGAGGTTACATTACGTATTCG‑TACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGAGGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCAt < 2:511419/150‑1 (MQ=255) ttCTGCTATGGCGGTTACCGGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCG‑TACCTGGTGGGGCATCGTCATTTGTTTTAAAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCAt < 2:663013/150‑1 (MQ=255) tCTGCTATGGCGGTTACCGGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCG‑TACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCAtt < 2:112865/150‑1 (MQ=255) ttACCGGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCG‑TACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGt > 2:733927/1‑150 (MQ=255) aCCGGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCG‑TACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTAc < 1:258342/150‑1 (MQ=255) ggAGAGAAAAGGGGGTGAGGTTACATTACGTATTCG‑TACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCtt > 1:469714/1‑151 (MQ=255) agaAAAGGGGGTGAGGTTACATTACGTATTCG‑TACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTatat > 1:719/1‑151 (MQ=255) gggggTGAGGTTACATTACGTATTCG‑TACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTAc < 1:46161/151‑1 (MQ=255) ggggTGAGGTTACATTACGTATTCG‑TACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTACa < 1:387484/151‑1 (MQ=255) | GGACTGCGATTAATCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCG‑TACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTACA > NC_000913/1478174‑1478470 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |