Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,736,7890GC46.7% 42.6 / 11.4 31L168V (CTG→GTG) ydhPputative MFS transporter, inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (15/1);  new base C (0/14);  total (16/15)
Fisher's exact test for biased strand distribution p-value = 2.06e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAGCACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATGAGAAGAACAGACTTACCATTGAAATCACTCCCAGCCCCGCCGTTGCCAGAAATGACATCCGCCAGCCGATGGTTTCACCCAACCAGGTCGCCGCCGGCACGCCACCGATATTTGCCAGGGTTAACCCCATAAACATAGTGGCAACTGCGCTGGCCTGTTTATGTTTTGGCACCACGCTTGCGG  >  NC_000913/1736646‑1736938
                                                                                                                                               |                                                                                                                                                     
aCCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAGCACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATGAGAAGTACAGACTTACCATTGAAATCACTCCCATCCccgc                                                                                                                                                 >  1:344340/1‑150 (MQ=255)
aCCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAGCACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATGAGAAGAACAGACTTACCATTGAAATCACTCCCAGCCccgcc                                                                                                                                                >  2:21289/1‑151 (MQ=255)
   aGCTCCCAGTACCGTCGTCAGCAATGCAGACAGCACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATGAGAAGAACAGACTTACCATTGAAATCACTCCCAGCCCCGCCGtt                                                                                                                                             >  1:95359/1‑151 (MQ=255)
      tCCCAGTACCGTCGTCAGCAATGCAGACAGCACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATGAGAAGAACAGACTTACCATTGAAATCACTCCCAGCCCCGCCGTTGcc                                                                                                                                          >  1:257606/1‑151 (MQ=255)
            tACCGTCGTCAGCAATGCAGACAGCACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATGAGAAGAACAGACTTACCATTGAAATCACTCCCAGCCCCGCCGTTGCCAGAAAt                                                                                                                                    >  1:720189/1‑151 (MQ=255)
                    ccaccAATGCAGACAGCCCCCGCGGCCTCATTAATCCCGCCAGCTTTTTTTTCACTCCAGGTCGTCCCCCTCCCCCCCCTTTAGGTAATGAAAAAAACAGACTTCCCATTGAAACCACCCCCACCCCCGCCGTTGCCAGAAATGACATCCg                                                                                                                            <  1:164105/147‑1 (MQ=255)
                              agagcacccccgCGGACGCATTAAAACCGCCGGTTCTTTTTCCCCTAATGCTCGCCCCCCTCCACCCCCTATAGGTAAAGAGAGGAACAAATCACCCATAGAATTCCCCCCCACCCCCGCCGTTGCCAGAAATGACATCCGc                                                                                                                           <  2:506466/131‑1 (MQ=255)
                                           ggACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATGAGAAGAACAGACTTACCATTGAAATCACTCCCAGCCCCGCCGTTGCCAGAAATGACATCCGCCAGCCGATGGTTTCACCCAAcc                                                                                                     >  1:438063/1‑151 (MQ=255)
                                                cATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATGAGAAGAACAGACTTACCATTGAAATCACTCCCAGCCCCGCCGTTGCCAGAAATGACATCCGCCAGCCGATGGTTTCACCCAACCAGGTc                                                                                                >  1:592786/1‑151 (MQ=255)
                                                       cccccccccttttttttCCCTTCGGTTCGCCCCCCTCCCCCCCTTTTAGGTAATAAAAAAAACAGACTTACAATAAAACCCCCCCCCAGCCCCGCCGTTGCCAGAAATGACATCCGCCAGCCGATGGTTTCACCCAACCAGGTCGccgccg                                                                                         <  2:368540/140‑1 (MQ=255)
                                                         ccccAGCTCTTTTTCCCCTTCAGGTCGTCCCCCTCCACCACTTTTAGGTAATGAAAAGAACAGCCTTACCATGAAAATCCCCCCCACCCCCGCCGTTGCCAGAAATGACATCCGCCAGCCGATGGTTTCACCCAACCAGGTCGCCGCCGGc                                                                                       <  1:11114/149‑1 (MQ=255)
                                                           ccAGCTTTTTTTCCTCTCCAGGTCGCGCCCCTCCCCCCCTTTGAGAAAATGAGAAGAACAGTCTTCCCATTAAACCCCCCCCCACCCCCGCCGTTGCCAGAAATGACATCCGCCAGCCGATGGTTTCACCCAACCAGGTCGCCGCCGGCa                                                                                      <  2:745327/150‑1 (MQ=255)
                                                                             cgggTCGTCCCCCTCCCCCCCTTTTAGGAAATGAGAAAAACAGACTTCCCATTAAAACCCCCCCCACCCCCGCCGTTGCCAGAAATGACATCCGCCAGCCGATGGTTTCACCCAACCAGGTCGCCGCCGGCACGCCACCGATATTTGc                                                                      <  1:654191/146‑1 (MQ=255)
                                                                               ggTCGTCCCCCTGCACCCCCTTTAGGAAATGAAAAAAACAGACTTCCCATTGAAACCCCCCCCACCCCCGCCGTTGCCAGAAATGACATCCGCCAGCCGATGGTTTCACCCAACCAGGTCGCCGCCGGCACGCCACCGATATTTGCCAgg                                                                  <  1:742162/150‑1 (MQ=255)
                                                                               ggTCGCCCCCCTCCCCCCCTTTTAGGTAATGAGAAAAACAGACTTCCCATTGAAACCCCCCCCCCCCCCGCCGTTGCCAGAAATGACATCCGCCAGCCGATGGTTTCACCCAACCAGGTCGCCGCCGGCACGCCACCGATATTTGCCAgg                                                                  <  1:721511/150‑1 (MQ=255)
                                                                                                   tttAGGTAATGAGAAGAACAGACTTACCATTGAAATCACTCCCAGCCCCGCCGTTGCCAGAAATGACATCCGCCAGCCGATGGTTTCACCCAACCAGGTCGCCGCCGGCACGCCACCGATATTTGCCAGGGTTAACCCCATAAACATAGTg                                             >  1:730360/1‑151 (MQ=255)
                                                                                                    ttAGGTAATGAGAAGAACAGACTTACCATTGAAATCACTCCCAGCCCCGCCGTTGCCAGAAATGACATCCGCCAGCCGATGGTTTCACCCAACCAGGTCGCCGCCGGCACGCCACCGATATTTGCCAGGGTTAACCCCATAAACATAGTgg                                            >  2:598737/1‑151 (MQ=255)
                                                                                                     tAGGTAATGAGAAGAACAGACTTACCATTGAAATCACTCCCAGCCCCGCCGTTGCCAGAAATGACATCCGCCAGCCGATGGTTTCACCCAACCAGGTCGCCGCCGGCACGCCACCGATATTTGCCAGGGTTAACCCCATAAACATAGTGGc                                           >  1:792148/1‑151 (MQ=255)
                                                                                                       ggaaaggaaaacaaCAGCCTTCCCATTAAAACCCCCCCCACCCCCGCCGTTGCCAGAAATGACATCCGCCAGCCGATGGTTTCACCCAACCAGGTCGCCGCCGGCACGCCACCGATATTTGCCAGGGTTAACCCCATAAACATAGt                                              <  1:80011/134‑1 (MQ=255)
                                                                                                          aaggaaaaaaaCAGCCTCACCATTAAATCCCCCCCCACCCCCGCCGTTGCCAGAAATGACATCCGCCAGCCGATGGTTTCACCCAACCAGGTCGCCGCCGGCACGCCACCGATATTTGCCAGGGTTAACCCCATAAACATAGTGGCAACt                                       <  1:219671/141‑1 (MQ=255)
                                                                                                              agaagaACAGACTTACCATTGAAATCACTCCCAGCCCCGCCGTTGCCAGAAATGACATCCGCCAGCCGATGGTTTCACCCAACCAGGTCGCCGCCGGCACGCCACCGATATTTGCCAGGGTTAACCCCATAAACATAGTGGCAACTGCGCt                                  >  2:26174/1‑151 (MQ=255)
                                                                                                              aaaagaaCAGCTTTACCATTGAAATCCCCCCCCCCCCCGCCGTTGCCAGAAATGACATCCGCCAGCCGATGGTTTCACCCAACCAGGTCGCCGCCGGCACGCCACCGATATTTGCCAGGGTTAACCCCATAAACATAGTGGCAACTgcg                                    <  2:126969/147‑1 (MQ=255)
                                                                                                                 agaaCAGACTTACCATTGAAATCACTCCCAGCCCCGCCGTTGCCAGAAATGACATCCGCCAGCCGATGGTTTCACCCAACCAGGTCGCCGCCGGCACGCCACCGATATTTGCCAGGGTTAACCCCATAAACATAGTGGCAACTGCGCTGGc                               >  2:575663/1‑151 (MQ=255)
                                                                                                                 agaaCAGACTTACCATTGAAATCACTCCCAGCCCCGCCGTTGCCAGAAATGACATCCGCCAGCCGATGGTTTCACCCAACCAGGTCGCCGCCGGCACGCCACCGATATTTGCCAGGGTTAACCCCATAAACATAGTGGCAACTGCGCTGGc                               >  1:19446/1‑151 (MQ=255)
                                                                                                                     cAGACTTACCATTGAAATCACTCCCAGCCCCGCCGTTGCCAGAAATGACATCCGCCAGCCGATGGTTTCACCCAACCAGGTCGCCGCCGGCACGCCACCGATATTTGCCAGGGTTAACCCCATAAACATAGTGGCAACTGCGCTGGCCTGt                           >  1:471081/1‑151 (MQ=255)
                                                                                                                             ccataaaacccccccccACCCCCGCCGTTGCCAGAAATGACATCCGCCAGCCGATGGTTTCACCCAACCAGGTCGCCGCCGGCACGCCACCGATATTTGCCAGGGTTAACCCCATAAACATAGTGGCAACTGCGCTGGCCTGTTTATGt                     <  2:345638/135‑1 (MQ=255)
                                                                                                                              cATTGAAATCACTCCCAGCCCCGCCGTTGCCAGAAATGACATCCGCCAGCCGATGGTTTCACCCAACCAGGTCGCCGCCGGCACGCCACCGATATTTGCCAGGGTTAACCCCATAAACATAGTGGCAACTGCGCTGGCCTGTTTATGtttt                  >  2:87070/1‑151 (MQ=255)
                                                                                                                                ttaaaaccccccccACCCCCGCCGTTGCCAGAAATGACATCCGCCAGCCGATGGTTTCACCCAACCAGGTCGCCGCCGGCACGCCACCGATATTTGCCAGGGTTAACCCCATAAACATAGTGGCAACTGCGCTGGCCTGTTTATGtttt                  <  1:598784/138‑1 (MQ=255)
                                                                                                                                      ccccccccACCCCCGCCGTTGCCAGAAATGACATCCGCCAGCCGATGGTTTCACCCAACCAGGTCGCCGCCGGCACGCCACCGATATTTGCCAGGGTTAACCCCATAAACATAGTGGCAACTGCGCTGGCCTGTTTATGTTTTGGcaccac          <  1:709924/146‑1 (MQ=255)
                                                                                                                                           cccACCCCCGCCGTTGCCAGAAATGACATCCGCCAGCCGATGGTTTCACCCAACCAGGTCGCCGCCGGCACGCCACCGATATTTGCCAGGGTTAACCCCATAAACATAGTGGCAACTGCGCTGGCCTGTTTATGTTTTGGCACCACGCtt      <  1:809503/150‑1 (MQ=255)
                                                                                                                                              aGCCCCGCCGTTGCCAGAAATGACATCCGCCAGCCGATGGTTTCACCCAACCAGGTCGCCGCCGGCACGCCACCGATATTTGCCAGGGTTAACCCCATAAACATAGTGGCAACTGCGCTGGCCTGTTTATGTTTTGGCACCACGCTTGCgg  >  2:303884/1‑151 (MQ=255)
                                                                                                                                               |                                                                                                                                                     
ACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAGCACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATGAGAAGAACAGACTTACCATTGAAATCACTCCCAGCCCCGCCGTTGCCAGAAATGACATCCGCCAGCCGATGGTTTCACCCAACCAGGTCGCCGCCGGCACGCCACCGATATTTGCCAGGGTTAACCCCATAAACATAGTGGCAACTGCGCTGGCCTGTTTATGTTTTGGCACCACGCTTGCGG  >  NC_000913/1736646‑1736938

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑