Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,230,727	C→A	intergenic (‑333/‑40)	hlyE ← / → umuD	hemolysin E/translesion error‑prone DNA polymerase V subunit; RecA‑activated auto‑protease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,230,727	0	C	A	100.0%	163.6 / NA	43	intergenic (‑333/‑40)	hlyE/umuD	hemolysin E/translesion error‑prone DNA polymerase V subunit; RecA‑activated auto‑protease
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (20/23);  total (20/23)

CGGGTGATATTTTTCATTTCTGCTGGATGAGCGTCGTCGCCAGAAGGCCACGTGAGCACAAGATAAGAGAACGAAAAATCAGCAGCCTATGCAGCGACAAATATTGATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACA  >  NC_000913/1230583‑1230873                                                                                                                                                 |                                                                                                                                                   cGGGGGATATTTTTCATTTCTGCTGGATGAGCGGCGTCTCCAGAAGGCCACGTGAGCACAAGATAAGAGAACGAAAAATCAGCGGCCTATGCAGCGACAAATATTGATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGAATACTg                                                                                                                                               >  2:1679/1‑150 (MQ=255)  gggTGATATTTTTCATTTCTGCTGGATGAGCGTCGTCGCCAGAAGGCCACGTGAGCACAAGATAAGAGAACGAAAAATCAGCAGCCTATGCAGCGACAAATATTGATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGAATACTGta                                                                                                                                             <  2:745422/151‑1 (MQ=255)   ggTGATATTTTTCATTTCTGCTGGATGAGCGTCGTCGCCAGAAGGCCACGTGAGCACAAGATAAGAGAACGAAAAATCAGCAGCCTATGCAGCGACAAATATTGATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGAATACTGtat                                                                                                                                            >  1:768330/1‑151 (MQ=255)    gTGATATTTTTCATTTCTGCTGGATGAGCGTCGTCGCCAGAAGGCCACGTGAGCACAAGATAAGAGAACGAAAAATCAGCAGCCTATGCAGCGACAAATATTGATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGAATACTGtata                                                                                                                                           <  1:556746/151‑1 (MQ=255)       atatTTTTCATTTCTGCTGGATGAGCGTCGTCGCCAGAAGGCCACGTGAGCACAAGATAAGAGAACGAAAAATCAGCAGCCTATGCAGCGACAAATATTGATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGAATACTGtatata                                                                                                                                         <  1:660645/150‑1 (MQ=255)         atTTTTCATTTCTGCTGGATGAGCGTCGTCGCCAGAAGGCCACGTGAGCACAAGATAAGAGAACGAAAAATCAGCAGCCTATGCAGCGACAAATATTGATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGAATACTGTATATaaa                                                                                                                                       >  1:454477/1‑150 (MQ=255)                    ctgctgGATGAGCGTCGTCGCCAGAAGGCCACGTGAGCACAAGATAAGAGAACGAAAAATCAGCAGCCTATGCAGCGACAAATATTGATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGAATACTGTATATAAAAACAGTATAACt                                                                                                                           >  1:402883/1‑151 (MQ=255)                           aTGAGCGTCGTCGCCAGAAGGCCACGTGAGCACAAGATAAGAGAACGAAAAATCAGCAGCCTATGCAGCGACAAATATTGATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGAATACTGTATATAAAAACAGTATAACTTCAGGc                                                                                                                     >  2:659117/1‑150 (MQ=255)                                      cgCCAGAAGGCCACGTGAGCACAAGATAAGAGAACGAAAAATCAGCAGCCTATGCAGCGACAAATATTGATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGAATACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTAtgt                                                                                                         <  2:495574/151‑1 (MQ=255)                                                 cACGTGAGCACAAGATAAGAGAACGAAAAATCAGCAGCCTATGCAGCGACAAATATTGATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGAATACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAg                                                                                              <  1:297172/151‑1 (MQ=255)                                                 cACGTGAGCACAAGATAAGAGAACGAAAAATCAGCAGCCTATGCAGCGACAAATATTGATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGAATACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAg                                                                                              <  2:554968/151‑1 (MQ=255)                                                   cGTGAGCACAAGATAAGAGAACGAAAAATCAGCAGCCTATGCAGCGACAAATATTGATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGAATACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGcc                                                                                            <  2:321536/151‑1 (MQ=255)                                                               aTAAGAGAACGAAAAATCAGCAGCCTATGCAGCGACAAATATTGATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGAATACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCcg                                                                                <  2:809935/151‑1 (MQ=255)                                                               aTAAGAGAACGAAAAATCAGCAGCCTATGCAGCGACAAATATTGATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGAATACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCcg                                                                                <  2:461004/151‑1 (MQ=255)                                                                      aaCGAAAAATCAGCAGCCTATGCAGCGACAAATATTGATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGAATACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAAtt                                                                         <  2:551638/151‑1 (MQ=255)                                                                           aaaaTCAGCAGCCTATGCAGCGACAAATATTGATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGAATACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGAc                                                                    >  2:638024/1‑151 (MQ=255)                                                                              aTCAGCAGCCTATGCAGCGACAAATATTGATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGAATACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACttt                                                                 <  1:577861/151‑1 (MQ=255)                                                                                cagcagCCTATGCAGCGACAAATATTGATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGAATACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTc                                                               >  1:271188/1‑151 (MQ=255)                                                                                      ccTATGCAGCGACAAATATTGATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGAATACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTAt                                                         >  1:731320/1‑151 (MQ=255)                                                                                         aTGCAGCGACAAATATTGATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGAATACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTa                                                      <  1:607680/151‑1 (MQ=255)                                                                                              gCGACAAATATTGATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGAATACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGAt                                                 >  1:558002/1‑151 (MQ=255)                                                                                               cGACAAATATTGATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGAATACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATc                                                <  1:368365/151‑1 (MQ=255)                                                                                                 aCAAATATTGATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGAATACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCtt                                              >  1:340372/1‑151 (MQ=255)                                                                                                 aCAAATATTGATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGAATACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCtt                                              >  2:417425/1‑151 (MQ=255)                                                                                                 aCAAATATTGATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGAATACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCt                                               <  1:465073/150‑1 (MQ=255)                                                                                                   aaaTATTGATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGAATACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTg                                             <  2:266519/150‑1 (MQ=255)                                                                                                     atatTGATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGAATACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTc                                          >  1:391448/1‑151 (MQ=255)                                                                                                       atTGATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGAATACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTc                                          >  1:759192/1‑149 (MQ=255)                                                                                                         tGATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGAATACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAgtg                                      >  2:281107/1‑151 (MQ=255)                                                                                                            tAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGAATACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTgg                                   >  1:381047/1‑151 (MQ=255)                                                                                                               ccTGAATCAGTATTGATCTGCTGGCAAGAACAGAATACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCtt                                <  2:58103/151‑1 (MQ=255)                                                                                                                    aTCAGTATTGATCTGCTGGCAAGAACAGAATACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCtt                           >  2:270629/1‑151 (MQ=255)                                                                                                                        gTATTGATCTGCTGGCAAGAACAGAATACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCAcc                       <  2:509986/151‑1 (MQ=255)                                                                                                                            tGATCTGCTGGCAAGAACAGAATACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGgc                    <  1:75365/150‑1 (MQ=255)                                                                                                                              aTCTGCTGGCAAGAACAGAATACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGgcagc                 >  1:25553/1‑151 (MQ=255)                                                                                                                                     ggCAAGAACAGAATACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTa           <  2:680392/150‑1 (MQ=255)                                                                                                                                     ggCAAGAACAGAATACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTa           <  1:34539/150‑1 (MQ=255)                                                                                                                                        aaGAACAGAATACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGtt       >  2:421553/1‑151 (MQ=255)                                                                                                                                           aaCAGAATACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGaa    <  2:786562/151‑1 (MQ=255)                                                                                                                                           aaCAGAATACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGaa    <  2:443387/151‑1 (MQ=255)                                                                                                                                             cAGAATACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAAc   >  2:651971/1‑150 (MQ=255)                                                                                                                                             cAGAATACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACa  <  1:185505/151‑1 (MQ=255)                                                                                                                                              aGAATACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAAc   >  2:465033/1‑149 (MQ=255)                                                                                                                                                 |                                                                                                                                                   CGGGTGATATTTTTCATTTCTGCTGGATGAGCGTCGTCGCCAGAAGGCCACGTGAGCACAAGATAAGAGAACGAAAAATCAGCAGCCTATGCAGCGACAAATATTGATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACA  >  NC_000913/1230583‑1230873

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑