Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,483,268 | 0 | T | A | 43.4% | 48.1 / 25.8 | 53 | L24F (TTA→TTT) | emrK | multidrug resistance efflux pump membrane fusion protein |
Reads supporting (aligned to +/- strand): ref base T (15/15); new base A (0/23); total (15/38) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 2.88e-05 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAACACAGTGC > NC_000913/2483120‑2483417 | tCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCaataaata < 1:615241/150‑1 (MQ=255) cGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAataaataa > 2:773565/1‑150 (MQ=255) tAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATa > 2:814729/1‑150 (MQ=255) aCAGGGACACTCCCTGAGCTTTGTGAAAAAATTGGTTCTCCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCAATTCCCATTGACCAAAAGGCATAGGCACAGAAAAACGCAATAAAAAAACCTACCGCCAATAAAGa < 1:958296/149‑1 (MQ=255) cAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGaaa > 2:341700/1‑150 (MQ=255) aGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGaaa > 1:399350/1‑149 (MQ=255) gTGACACTCCCTGAGACTTGTGCAAAAATTGGATCTGCATCCCCCGTGACATAGGCGTCATCTGTCCTAATCATGTCTTCTAATTCCATTGACCAAAAGGCATAGGCACCTAAAAACGCAATAAATAAAACTACCGCCAATAAAGaaaa < 2:724254/149‑1 (MQ=255) ttACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTttc > 1:210473/2‑151 (MQ=255) ccggagaCTTGTGCAAAAATTGGATCGGCATTCCCCGTGAAATAGGCGTCATCTGTAATAACCATGTCTTCTAATCCCATTAACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAACCTACCGCCAATAAAGAAAAGTATTttctt < 1:417835/147‑1 (MQ=255) ccTGAGTCTTGTGCAAAAATTGGCTCTCCATTCCCCGTGACATAGGCGTCATCTGTCCTAATCATGTCTTCAAATTCCATTGCCCAAAAGAAAAAGGCCCCTAAAACCCCAAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTttct < 1:241464/149‑1 (MQ=255) ccTGAGATTTGTGCAAAAATTGTATCTGCATTCCCCGTGACATGGGCGTCTTCTTTACTAATCATGTCTTCTAATCCCATTGACCAATAGGCATAGGCACCTAAAACCGCAAAAAATAAAACTACCGCCAATAAAGAAAAGTATTttcttc < 2:466412/151‑1 (MQ=255) ccTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTttcttc > 2:848213/1‑151 (MQ=255) ccTGAGACTTGTGCAGAAATGGGATCTGCTTTCCCCGTGACATAGGCGTCATCTGTACTAACAATGTCTTCAAATCCCATTGACCAATAGGCATAGGCACCTGAAAACGCAAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTttct < 2:83128/149‑1 (MQ=255) ttGTGCAAAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTAAAACCGCAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGa < 2:727122/150‑1 (MQ=255) gtgCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATCCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGaa < 2:269077/149‑1 (MQ=255) aGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGAAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGtttt < 2:496432/151‑1 (MQ=255) aaaTTGGATCTGCATTCCCCTTGACATAGGCGTCATCTGTACTAATCATTTTTTCAAATTCCATTAACCAATAGGCATAGGCCCCTGAAACCGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGttttt < 2:896853/150‑1 (MQ=255) aaaTTGGATCTGCATCCCCCGTGACATAGGCGTCATCTTTACAAATCATTTCTTCTATTCCCATTGACCAAGAGGCATAGGCACCTAAAAACGCAAAAAAAAAAATTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGt < 2:211091/146‑1 (MQ=255) aTCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGa > 1:584116/1‑150 (MQ=255) gctttCCCCGTGACAAAGGCGTCATCTTTACTAATAATTTCTTCAAATCCCATTACCAAAAAGGCAAAGGCCCTTGAAAACGAAATAAAAAAAACTCCCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGa < 2:440727/143‑1 (MQ=255) aTTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATc > 2:366019/1‑151 (MQ=255) ccccGTGACATGGGCGTCATCTTTACTAATCATGTCTTCTAATTCCATTGACCAAAAGACATAGGCACCTAAAAACGAAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAAtt < 1:271620/144‑1 (MQ=255) ccccGTGACATAGGCGTCATCTTTACTAATCATTTCTTCTAATCCCATTGACAAATAGGCATGGGCCCTGGAAACCGCAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGt < 2:677360/151‑1 (MQ=255) ccccGGGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTAAAAACGAAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCt < 2:469811/149‑1 (MQ=255) gACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACAAATAGGCATAGGCACCTAAAAACGAAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGCTCCa < 2:200896/149‑1 (MQ=255) aaCATAGCCGTAATCTGAACTAACCATGCCACATAATCCCATTAACAAAAGTGCTTAGAACCTTAAAAACCAAAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTcc < 1:356903/147‑1 (MQ=255) aCATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACt > 2:238140/1‑150 (MQ=255) ggCGTCATCTGTACAATTCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTAAAAACGCAATAAAAAAAATTGCCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACtatta < 2:62276/149‑1 (MQ=255) gCGTCATCTGTACTAATCATGTCTTCAAATTCCATGAACCAATAGGCATAGCCACCAAAAAACGCAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTAtct < 2:94547/151‑1 (MQ=255) tGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTtc > 2:239840/1‑151 (MQ=255) tACTAATCATGTTTTCAAATTCCATTAACAAATAGGAATAGCCACCTAAAAACAAAAAAAATAAATCTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCAtt < 1:466081/147‑1 (MQ=255) acaaaTCATTTCTTATAATTCCATTGACAAATAGGAATAGCCACCTAAAAACGAAAAAAATAAAATTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTtctc < 1:4827/147‑1 (MQ=255) aaTCATGTCTTCTAATTCCATTGACAAATAGGCATAGGCACCTGAAAACGAAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTtctc < 2:822396/147‑1 (MQ=255) ttcttctAATTCCATTGACCAATAGGCATAGGCACCTAAAAACGAAATAAATAAATTTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGaa < 1:187464/149‑1 (MQ=255) tcttctAATTCCATTGACCAATAGGCATGGGCACCTGAAACCGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATg < 2:105697/147‑1 (MQ=255) tttAATTCCATTGACCAAAAGGCAGATGCACCTGAAAATGAAATATATAATAGTACCGACAATAAAGAAAAAAATTTTCTTCTGTTAGAATGTTTTTTTTTTGAATTAATCTCTTCCATTATTATCTCTCATATCTCATAGATTAAAtt > 1:599275/3‑149 (MQ=255) attaccagtgaaaaaTAGGCGTGGGCTCCTTACACCGAAAAAAAAAAAACTGCCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTGATTTCTCATAGATGAACTTTa < 1:542416/135‑1 (MQ=255) aaTTCCATTGACCAATAGGCATAGCCACCTAAAAACCAAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTAtg < 2:884055/150‑1 (MQ=255) aaTTCAATTGACCAATAGGAATAATCACCTAAAAACCAAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGaaa < 1:221160/144‑1 (MQ=255) aTTGACAAATAGGCATAGGCACCTGAAACCGAAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTAtgaattgaa < 2:399393/151‑1 (MQ=255) aTTCACCAATAGAAAAAGGCCCCTAAAAACGAAAAAAAAAAATCTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTAtga < 1:536824/145‑1 (MQ=255) gACCAATAGGCATAGGCACCTGAAAACGCAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTAtgaattgaat < 2:159376/149‑1 (MQ=255) gACCAATAGGAATAGGCACCTGAAAACGAAAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATtgagtg < 2:825487/150‑1 (MQ=255) ccAATAGGCATAGGCACCTAAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATtgaatg < 2:825479/148‑1 (MQ=255) cAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGaaa > 2:314839/1‑150 (MQ=255) atagacatatgcACCTAAAAACAAAAAAAATAAAATTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGaaa < 1:289824/148‑1 (MQ=255) taggcataggcaCCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAag > 2:282929/1‑149 (MQ=255) ggcataggcaCCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGc > 1:43560/1‑150 (MQ=255) ggcataggcaCCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGc > 1:928277/1‑150 (MQ=255) atagcccCCTAAAAACGCAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGcat < 2:550789/149‑1 (MQ=255) taggcaCCTGAAAACGCAATAAATAAAACTACCGCCAATGAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGcattc > 1:225165/1‑150 (MQ=255) ggcaCCTAAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGcattcatt < 2:252500/151‑1 (MQ=255) ccTAAAACCGAAATAAATAAACCTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCAttctt < 2:756467/150‑1 (MQ=255) aaaaCGAAAAAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCatat < 2:515729/151‑1 (MQ=255) aaaaCGAAAAAAAAAAAATTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCAttcttc < 2:837903/147‑1 (MQ=255) aataaataAAACTACCGCCAATGAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAac < 2:225205/149‑1 (MQ=255) aataaataAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAacac < 2:21815/151‑1 (MQ=255) aataaaaaAAATTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAaca < 1:396210/150‑1 (MQ=255) aataaaaaAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAaca < 2:691547/150‑1 (MQ=255) aataaaaaAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAac < 1:766336/149‑1 (MQ=255) aaaaaataaaACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCTCTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCaaa < 2:454340/145‑1 (MQ=255) aaataaaATTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAACACAGt < 2:737344/151‑1 (MQ=255) aaataaaACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAACACAGt > 2:36172/1‑151 (MQ=255) ataaaACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAACACAGTGc > 2:659822/1‑151 (MQ=255) | TCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAACACAGTGC > NC_000913/2483120‑2483417 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |