Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,206,435	0	C	T	45.3%	59.2 / 42.9	64	intergenic (‑179/‑28)	ttdR/ttdA	transcriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (7/28);  new base T (29/0);  total (36/28)
Fisher's exact test for biased strand distribution p-value = 7.97e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GA‑ATTTTTCCAGACAAATACAA‑AAACTGGAGTT‑GCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCT‑AACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAA  >  NC_000913/3206293‑3206583                                                                                                                                                 |                                                                                                                                                        ttCGCAAAACATTGCCATTCCCTGCTCTGGCGGATTATTCATTTTTATTTTCGCGTTGATTCACGACCTGTTCATTACATATTCACGGCGGTAACAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGC‑CG‑ATTTTTcccact                                                                                                                                                    >  1:323482/1‑144 (MQ=255)  tCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑CA‑ATTTTTTCTGCCaaa                                                                                                                                                 >  2:284491/1‑150 (MQ=255)     cAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GA‑ATTTTTTTATACaaaaa                                                                                                                                               >  1:843951/1‑147 (MQ=255)      aaaaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GA‑ATTTTTTCAaaaaaaaa                                                                                                                                               >  1:169940/1‑146 (MQ=255)           aTTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GA‑ATTTTTCCAGACAAATACAA‑AAACt                                                                                                                                      <  2:932890/151‑1 (MQ=255)             tGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GA‑ATTTTTTCAGACAAATAaaa‑aaa                                                                                                                                        >  2:244640/1‑147 (MQ=255)             tGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GA‑ATTTTTCCAGACAAATACAA‑AAACTg                                                                                                                                     <  2:558391/150‑1 (MQ=255)             tGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GA‑ATTTTTCCAGACAAATACAA‑AAACTg                                                                                                                                     <  2:480100/150‑1 (MQ=255)              gCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GA‑ATTTTTCCAGACAAATACAA‑AAACTgg                                                                                                                                    <  2:735619/150‑1 (MQ=255)              gCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GA‑ATTTTTCCAGACAAATACAA‑AAACTGGa                                                                                                                                   <  2:174657/151‑1 (MQ=255)               ccATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GA‑AATTTTTTAAAAAAAAAAAA‑AAAATgg                                                                                                                                    >  1:665246/1‑149 (MQ=255)               ccATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATACAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GA‑ATTTTTCCAGACAAATACAA‑AAACTGGAg                                                                                                                                  <  1:120372/151‑1 (MQ=255)                   tCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GA‑ATTTTTCCAGACAAATACAA‑AAACTGGAGTT‑g                                                                                                                              <  1:101319/150‑1 (MQ=255)                    cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAACAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGC‑GT‑CTTTTTCTATACaaaaacaa‑aaacaataggtggc                                                                                                                             >  1:718534/1‑141 (MQ=255)                      ttGCTCTGGCGCAATATTAATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAGATTTTTTCA‑ACAaaaaaaa‑aaa                                                                                                                                        >  1:507205/2‑138 (MQ=255)                          tctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GA‑AATTTTTCAGAAAAAAACAA‑AAAACGGAGTT‑GCCatgat                                                                                                                       >  2:556370/1‑150 (MQ=255)                             ggCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GA‑ATTTTTTTCGACAAATACAA‑AAAACGGAGTT‑GGCatga                                                                                                                        >  2:738700/1‑146 (MQ=255)                               cgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGG‑GG‑ATTTTTTAAAACAAAAAAAA‑AAAAATGAGTT‑TTCATTATGAgagg                                                                                                                 >  1:906187/1‑148 (MQ=255)                                gcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GA‑ATTTTTTCATACAAAAACAA‑AAACTGGAGTT‑TTCATGGTGAg                                                                                                                    >  1:560497/1‑147 (MQ=255)                                  aaTATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GA‑ATTTTTTTAAACAAAAAAAA‑AAAAATGAGTT‑GGCATGATGAGCGgaa                                                                                                               >  2:927874/1‑150 (MQ=255)                                  aaTATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GA‑ATTTTTTCAGAAAAAAAAAA‑AAAATGGAGAT‑GGCATGAagaggggaa                                                                                                               >  2:550043/1‑145 (MQ=255)                                     attcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GA‑ATTTTTCCAGACAAATACAA‑AAACTGGAGTT‑GCCATGATGAGCGAAAGtaa                                                                                                           <  2:741260/151‑1 (MQ=255)                                     attcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GA‑ATTTTTCCAGACAAATACAA‑AAACTGGAGTT‑GCCATGATGAGCGAAAGtaa                                                                                                           <  1:794454/151‑1 (MQ=255)                                         attcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GA‑ATTTTTTCAGACAAAAACAA‑AAAAAGGAGTT‑GGCATGGTGAGGGAAAGtaataa                                                                                                        >  1:102736/1‑150 (MQ=255)                                          ttcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GA‑AATTTTTCATAAAAAAAACA‑AAAATGGGGTG‑TCCATGGTGGGGGAAAGgaataa                                                                                                        >  2:103152/1‑149 (MQ=255)                                           tcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GA‑ATTTTTTCAGACAAATAAAA‑AAAATTGAGGT‑GTCATGATGAGCGGAAAgaataa                                                                                                        >  2:145064/1‑148 (MQ=255)                                           tcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATGCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GA‑ATTTTTCCAGACAAATACAA‑AAACTGGAGTT‑GCCATGATGAGCGAAAGTAATAAGc                                                                                                      <  2:194019/150‑1 (MQ=255)                                            cTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGA‑ATTTTTTC‑GACAAAAAAAA‑AAAATGGAGTT‑GGCATGATGAGGGCAAGTAAAAAACa                                                                                                     >  1:30765/1‑150 (MQ=255)                                             ttATTTTCGCGTTGATTAACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGA‑GA‑ATTTTTTCCAACAAAAAAAATAAACGGAATTG‑GACAT‑ATGAGCGAAAGGAAAAAACaa                                                                                                    >  1:922337/1‑150 (MQ=255)                                              tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GA‑ATTTTTCCAGACAAATACAA‑AAACTGGAGTT‑GCCATGATGAGCGAAAGTAATAAGCAAc                                                                                                   <  2:714670/150‑1 (MQ=255)                                                      cgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GA‑ATTTTTCCAGACAAATACAA‑AAACTGGAGTT‑GCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGa                                                                                          <  1:251844/151‑1 (MQ=255)                                                          gATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GA‑ATTTTTCCAGACAAATACAA‑AAACTGGAGTT‑GCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATa                                                                                       <  2:358342/150‑1 (MQ=255)                                                           aTTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGG‑GA‑ATTTTTTCCAACAAATACAA‑AAAATGGAGGT‑GCCCAGATGGGCGAAAGAAAAAAGCAAAAAGCAGTGAaaaa                                                                                      >  2:40072/1‑150 (MQ=255)                                                              cACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GA‑ATTTTTCCAGACAAATACAA‑AAACTGGAGTT‑GCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTg                                                                                  <  2:102770/151‑1 (MQ=255)                                                              cACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GA‑AATTTTTCCCACAAATAAAA‑AAAATAGAGTT‑TCCATGATGAGGGGAAGAAAAAAACAAAAGGCAGGGAaaaa                                                                                      >  2:247554/1‑147 (MQ=255)                                                                cGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GA‑ATTTTTCCAGACAAATACAA‑AAACTGGAGTT‑GCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGAc                                                                                <  1:623501/151‑1 (MQ=255)                                                                 gAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGG‑GG‑AATTTTTCAGACAAAAACAA‑AAAATGGGGGT‑TGCATGATGAGGGAAAGAAATAAACAAAAAGGAGGGAATAAAtt                                                                                   >  1:320734/1‑147 (MQ=255)                                                                 gAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGC‑GG‑ATTTTTTCAGACAAAAACAA‑AAAATGGAGTT‑GCCATGATGAGCGGAAGGAATAAAAAACAAGCAGGGAATAAATTg                                                                                  >  1:287319/1‑148 (MQ=255)                                                                   aCTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GA‑ATTTTTCCAGACAAATACAA‑AAACTGGAGTT‑GCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACag                                                                              <  2:385220/150‑1 (MQ=255)                                                                               atatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGG‑GA‑AATTTTTCAAACAAAAACAA‑AAAATGGAGTT‑GTGATGATTAGGGAAAAGAATTAAAAACAAGCAGTGGATAAGTTGACAGAGAATTTCg                                                                     >  1:220245/1‑147 (MQ=255)                                                                                    cACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GA‑ATTTTTCCAGACAAATACAA‑AAACTGGAGTT‑GCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCT‑AACttt                                                            <  2:855244/150‑1 (MQ=255)                                                                                    cACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GA‑ATTTTTCCAGACAAATACAA‑AAACTGGAGTT‑GCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGGCGCT‑AACttt                                                            <  1:408230/150‑1 (MQ=255)                                                                                       ggTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GA‑ATTTTTCCAGACAAATACAA‑AAACTGGAGTT‑GCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCT‑AACTTTACCg                                                        <  2:326811/151‑1 (MQ=255)                                                                                       ggTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GA‑ATTTTTCCAGACAAATACAA‑AAACTGGAGTT‑GCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCT‑AACTTTACCg                                                        <  2:444572/151‑1 (MQ=255)                                                                                        gTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GA‑ATTTTTTCAGACAAAAACAA‑AAAATGGAGGT‑GCCATGATGAGGGGAAGTAATAAACAACAGGCAGGGAATAAGTTGACAGAGATTGTCGCT‑AACTTTAcccc                                                       >  2:565651/1‑149 (MQ=255)                                                                                            cAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GA‑ATTTTTCCAGACAAATAAAA‑AAACTGGAGTT‑GCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCT‑AACTTTACCGCCAt                                                    <  1:856853/150‑1 (MQ=255)                                                                                                aaaTATAAAACCACATTTTTAGTGTTAGTTTGTGGCGGT‑GA‑ATTTTTCCAGAAAAATACAA‑AAACTGGAGTT‑GCGATGATGAGGGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCT‑AACTTTACCGCCATGAttt                                               <  1:228333/151‑1 (MQ=255)                                                                                                aaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GA‑ATTTTTCCAGACAAATACAA‑AAACTGGAGTT‑GCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTAAATAAGTGGACAGAGATTGTCGCT‑AACTTTACCGCCATGAttt                                               <  2:785175/151‑1 (MQ=255)                                                                                                   tataAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GA‑ATTTTTTCAGACAAATACAA‑AAAATGGAGTT‑GCCATGATGGGCGGAAATAATAAGCAACAGGCAGGGAATAAATTGGCAGAGATTGTCGCT‑AACTTTACCGCCATGAttttt                                             >  2:722324/1‑148 (MQ=255)                                                                                                      aaaaCCACATTTTTAGTGGTAGTTTGTGGCGGT‑GA‑ATTTTTCCAGACAAATACAA‑AAACTGGAGTT‑GCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCT‑AACTTTACCGCCATGATTTCTAccc                                         <  2:521378/151‑1 (MQ=255)                                                                                                         aCCACATTTTTAGTGGTAGTTTGTGGCGGT‑GA‑ATTTTTTCAGACAAAAACAA‑AAACTGGAGGT‑TGCATGGTGAGCGAAAGGAATAAGCAAAAGGCAGGGAATAAATTGACAGAGATTGTTGCT‑AACTTTAACGCCCTGATTTTTAccc                                         >  1:309863/1‑148 (MQ=255)                                                                                                          ccACATTTTTAGTGGTAGTTTGTGGCGGT‑GA‑ATTTTTTCCGACAAATAAAA‑AAAATGGAGTT‑GCCATGATGAGGGGAAGTAATAAACAACAGGCAGTGAATAAAGTGACAGAGATTGTCGCT‑AAATTTACCGCCCTGATTTCTAccc                                         >  2:76715/1‑147 (MQ=255)                                                                                                          ccACATTTTTAGTGGTAGTTTGTGGCGGT‑GA‑AATTTTTCAGACAAAAACAA‑AAACTGGAGTT‑GGCATGATGAGGGAAAGGAAAAAAAAAAAGGGCGTGAATAAATTGAAAGAGATTGTCGCT‑AAATTTACCGCCCAGATTTTTACCCGaa                                      >  2:429242/1‑150 (MQ=255)                                                                                                               tttttAGTGGTAGTTTGTGGCGGT‑GA‑GTTTTTTCAGAACAATAAAA‑AAAATGGTGGT‑GTCATGGTTGGCGGAAGGAAAAAACAAAAAGCCGTGAATAAAATGACAGAGATTGTCGCC‑TAATTTTCCGcccctcgtttttccc                                         >  1:536801/1‑129 (MQ=255)                                                                                                               tttttAGGGGTAGTTTGTGGGGGA‑GA‑ATTTTACCAAAAAAATAAAA‑AAACGGGAGTG‑GCCATGATGAGCAAAAATAAAAAGCAACAGGCAGTAAATAAGTTCACAGATATTTCCGTT‑AAGTTTCCCGCCATGATTTCTACCCGAATGCCt                                 <  1:323089/150‑1 (MQ=255)                                                                                                                ttttAGTGGTAGTTTGTGGCGGT‑GA‑ATTTTTTCCCACAAAAACAA‑AAACTGGAGTT‑GCCATGATGAGCGAAAGGAATAAGCAACAAGCAGAGAATAAGTTGACAGAGAATGTCGCT‑AACATTACCCCCATGATTTTTTCCCCAATGCCtg                                >  2:335085/1‑150 (MQ=255)                                                                                                                  ttAGTGGTATTTGGTGGCTTT‑GA‑TTTGTTCCAGACAGATA‑AA‑AACGTGGGGTTGGCCATGATGGGGGAAAGTAATAACAAAGAGGTAGGTAATAGGGTGAATGAGATT‑TCGGTGATTTTTACCGCCATGATTTCTACCCGAATGGCtgat                              <  1:568243/150‑1 (MQ=255)                                                                                                                           ttttGGGGGGGT‑AA‑ATTTTTCCAGAAAAATAAAA‑AAACTGGTGTT‑GCATTGATGACCGAAATAAATAAGCAACACGCATTGAATAATTTGAGAGAGGTTGTCGGT‑GTCTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgtggtg                     <  1:280381/149‑1 (MQ=255)                                                                                                                             ttGTGGCGGT‑GA‑ATTTTTCCAGACAAATAAAA‑AAACTGGAGTT‑GCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCT‑AACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa                   <  1:145026/150‑1 (MQ=255)                                                                                                                                tgGCGGT‑GA‑ATTTTTCCAGACAAATACAA‑AAACTGGAGTT‑GCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCT‑AACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATaa                >  2:291668/1‑150 (MQ=255)                                                                                                                                   cGGT‑GA‑ATTTTTCCAGACAAATACAA‑AAACTGGAGTT‑GCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCT‑AACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACt             >  2:844747/1‑150 (MQ=255)                                                                                                                                     gT‑GA‑ATTTTTCCAGACAAATACAA‑AAACTGGAGTT‑GCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCT‑AACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaa           >  1:630627/1‑150 (MQ=255)                                                                                                                                      t‑GA‑ATTTTTCCAGACAAATACAA‑AAACTGGAGTT‑GCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCT‑AACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaa          >  1:841872/1‑150 (MQ=255)                                                                                                                                             ttttCCAGACAAATACAA‑AAACTGGAGTT‑GCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCT‑AACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCt    >  2:775007/1‑151 (MQ=255)                                                                                                                                              tttCCAGACAAATACAA‑AAACTGGAGTT‑GCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCT‑AACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCt    >  1:194349/1‑150 (MQ=255)                                                                                                                                                 ccAGACAAATACAA‑AAACTGGAGTT‑GCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCT‑AACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTaa  >  2:842455/1‑149 (MQ=255)                                                                                                                                                 |                                                                                                                                                        TTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GA‑ATTTTTCCAGACAAATACAA‑AAACTGGAGTT‑GCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCT‑AACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAA  >  NC_000913/3206293‑3206583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑