Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913599,5760TG40.7% 100.3 / 19.1 59V288G (GTG→GGG) cusAcopper/silver efflux system, membrane component
Reads supporting (aligned to +/- strand):  ref base T (4/31);  new base G (24/0);  total (28/31)
Fisher's exact test for biased strand distribution p-value = 9.47e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCATCGACAA  >  NC_000913/599429‑599723
                                                                                                                                                   |                                                                                                                                                   
cGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAACTGGCGTGCCCGTTTATCTGCGCGATTTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGgggggggggg                                                                                                                                                   >  1:916118/1‑150 (MQ=255)
cGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGGGCCCGTTTATCTGCCCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGACGTGGCGGGCGggggggg                                                                                                                                                  >  1:309143/1‑150 (MQ=255)
   tCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGgggggggg                                                                                                                                                 >  1:365863/1‑147 (MQ=255)
        gacTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGc                                                                                                                                          <  1:444138/151‑1 (MQ=255)
             tAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTCCTGCGATcc                                                                                                                                     >  1:829177/1‑151 (MQ=255)
               aTCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGACCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGTGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCgg                                                                                                                                   <  1:179210/151‑1 (MQ=255)
                tCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGgggggggggggggtgccgggcgcc                                                                                                                                     >  1:945180/1‑134 (MQ=255)
                    aTCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGATCCTGCGATCCGGCaa                                                                                                                                >  1:112474/1‑149 (MQ=255)
                    aTCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCaaaa                                                                                                                              <  2:668826/151‑1 (MQ=255)
                       gTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCTGCGATCCCGCaaaaa                                                                                                                             >  2:353695/1‑149 (MQ=255)
                           tAAAAGCCAGTGAAAATGGCGTGCCCGGTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTTCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGTGAAACTCCGACTCAGCAAAAAccgccc                                                                                                                       >  2:677501/1‑146 (MQ=255)
                            aaaaGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCg                                                                                                                       <  2:106965/150‑1 (MQ=255)
                             aaaGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCgag                                                                                                                     <  1:508753/151‑1 (MQ=255)
                              aaGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCgaga                                                                                                                    <  1:917421/151‑1 (MQ=255)
                              aaGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATACCGCGAGGCGGCGAAAACGcccc                                                                                                                       >  1:691426/1‑147 (MQ=255)
                                 ccAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGAGCCTGCGATCCGGGAAAAACGccccgg                                                                                                                     >  1:661703/1‑144 (MQ=255)
                                     tGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGATGCCGCGGTCCGGGCAAAACAccccgggaggggt                                                                                                              >  1:522061/1‑140 (MQ=255)
                                      gAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGGTCCTGCGATCCCGCAAAAAAGCCCCCGAAGGGa                                                                                                               >  1:245438/1‑148 (MQ=255)
                                      gAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATcg                                                                                                            <  1:539737/151‑1 (MQ=255)
                                         aaTGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATcgccg                                                                                                         <  2:206495/151‑1 (MQ=255)
                                         aaTGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATcgccg                                                                                                         <  1:912338/151‑1 (MQ=255)
                                          aTGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTCCTGCGGTCCGGCAAAAAAGCCCGGGAAGGGATcgcc                                                                                                          >  2:912176/1‑149 (MQ=255)
                                              cGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGTGGTCCTGCGATCCGGCCAAAAAGCCCGAGAAGAGATCGCCGCCGTg                                                                                                    >  2:507314/1‑151 (MQ=255)
                                               gTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGa                                                                                                   <  2:151352/151‑1 (MQ=255)
                                               gTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCgccgcc                                                                                                       >  2:249345/1‑147 (MQ=255)
                                                        tATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGGGAAGGACaaa                                                                                           >  2:717116/1‑150 (MQ=255)
                                                           cTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGGGAATCCGCGATCCGGCAAAAACGCCCGAGAAGTGGTCGCCGCCGTGAAGGACAAACTgg                                                                                       >  2:301901/1‑151 (MQ=255)
                                                           cTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGAGCCTGCGATTCGGGAAAAAAACCCCAGAAGGGGTTGCCGCCGGGGAggggcaacttg                                                                                       >  1:218479/1‑142 (MQ=255)
                                                             gcgcGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTgg                                                                                       <  2:924051/149‑1 (MQ=255)
                                                             gcgcGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGaa                                                                                     <  2:444370/151‑1 (MQ=255)
                                                                cgATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGTGGTCCTGCGGTCCGGCCAAAAAGCCCGCGAAGGGATCGCCGCCGGGGAGGGCAAACCTGaaa                                                                                    >  1:832861/1‑149 (MQ=255)
                                                                cgATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTCCTGCGACTCGGCCAAAACGCCCCGGAAGAGATAGCCGCCCCGAAGGGCaaaac                                                                                         >  1:844639/1‑142 (MQ=255)
                                                                     ttGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGa                                                                              <  1:112499/150‑1 (MQ=255)
                                                                         gAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAagt                                                                         <  2:235405/151‑1 (MQ=255)
                                                                          aaGGTCCAGATTGGCCCGGAGATGCGCCGGGACATTGCCGAACTAAACGGCGAAGGCGAAGCGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGACCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAagt                                                                         <  2:171903/150‑1 (MQ=255)
                                                                            ggTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAagtagt                                                                      <  2:748787/151‑1 (MQ=255)
                                                                                  gATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCCGCGATCCGGCAAAAACGCCCGAGAAGGGATCGCCGCCGTGAAGGACAAACTGGAAAAGCTGAAAAGTAGTCTGcc                                                                 >  2:60066/1‑150 (MQ=255)
                                                                                        cccGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGc                                                          <  2:102035/151‑1 (MQ=255)
                                                                                        cccGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGc                                                          <  2:280931/151‑1 (MQ=255)
                                                                                         cctgagaTGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGAACCTGGGAGCCGGCAAAAACACCCGCGGAGGGGACGCCCGCGGGGAGGGCAAAATGGGAAAACGGAAAAGGAATCTGCCGGGAGGCg                                                         >  1:5573/4‑151 (MQ=255)
                                                                                         ccGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCg                                                         <  1:321773/151‑1 (MQ=255)
                                                                                              gaTGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGgag                                                    <  2:150451/151‑1 (MQ=255)
                                                                                              gaTGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCTGCGATCCGGCAAAAACGCCCGAGAAGGGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTgg                                                      >  2:918328/1‑149 (MQ=255)
                                                                                               aTGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGaaa                                                                            <  2:320468/126‑1 (MQ=255)
                                                                                                tGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTCCTGCGAGCCGGCAAAAACGCCCGAGAAGGGAGCGCCGCCGCGGAGGACAAAACGGAAACGCCGCAAAGGAGTGTGCAGGGAGGCgggggg                                                    >  1:943510/1‑147 (MQ=255)
                                                                                                     cGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTa                                             <  1:138833/151‑1 (MQ=255)
                                                                                                            ttGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCTGCGATCCGGCAAAAACGCCCGGGGAGAGATCGCCGCCGGGAAGGACAAACTGGGAAAGCTGGAAAGGAGTTTGCCGGAAGGCGGGGGGAAAGGTacaa                                          >  2:215938/1‑147 (MQ=255)
                                                                                                             tGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGTGGTCCTGCGATCCGGCAAAAACGCCCGGGAAGGGATCGCCGCCGTGGAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGGAGGCGGGGGGATAGTTACAAAATa                                      >  1:49216/1‑150 (MQ=255)
                                                                                                                 gAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGGAAAGTAGTCTGCCGGAAGGCGTGGGGATAGTTACAAAATAc                                     >  2:658327/1‑147 (MQ=255)
                                                                                                                      aaaCGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGcagc                            <  2:90470/151‑1 (MQ=255)
                                                                                                                      aaaCGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGca                              <  2:38519/149‑1 (MQ=255)
                                                                                                                      aaaCGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGca                              <  2:675398/149‑1 (MQ=255)
                                                                                                                       aaCGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGGGATTGCCGCCGTGAAGGACAAACTCGAAACGCTGAAAAGGAGTCTGCCGGGAGGCGTGGAGGTAGTTTCAACATACGATCGcaacc                           >  2:129462/1‑151 (MQ=255)
                                                                                                                             gaaggcgaagTGGCGGGCGGGGGGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAAATGGAAAAGCTGAAAAGTAGTCTGCCGGAAGGCGGGGAGATAGTTACAACATACGATCGCAGCCAGCTc                      >  2:901546/1‑150 (MQ=255)
                                                                                                                               aggcgaagTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCAtt                   <  1:54060/151‑1 (MQ=255)
                                                                                                                                ggcgaagTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTg                  <  2:627054/151‑1 (MQ=255)
                                                                                                                                    aagTGGCGGGCGGGGGGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGGGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGAcc               >  1:862888/1‑150 (MQ=255)
                                                                                                                                      gTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACcgcg            <  2:801810/151‑1 (MQ=255)
                                                                                                                                         gcgggcggGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGcc          >  1:432069/1‑150 (MQ=255)
                                                                                                                                          cgggcgggGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCAt        <  2:18163/151‑1 (MQ=255)
                                                                                                                                          cgggcgggGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCAt        <  2:135060/151‑1 (MQ=255)
                                                                                                                                           gggcgggGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCATc       >  2:349480/1‑151 (MQ=255)
                                                                                                                                            ggcgggGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCATCg      <  1:205278/151‑1 (MQ=255)
                                                                                                                                                ggggggTGATCCGGCGATCCGGCAAAAACGCCCGAGAAGTGAGCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTTACCGCGCCATCGACaa  <  1:817331/151‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCATCGACAA  >  NC_000913/599429‑599723

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑