Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	925,900	0	A	G	40.9%	63.3 / 16.0	44	noncoding (72/88 nt)	serW	tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base A (3/23);  new base G (18/0);  total (21/23)
Fisher's exact test for biased strand distribution p-value = 1.29e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTCACTGCCGTACAGGCAGCTTAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAG‑CCACTCGGACACCTCA‑CCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTCAAGAAGAA  >  NC_000913/925753‑926049                                                                                                                                                     |                                                                                                                                                        gTTCACTGCCGTACAGGCAGCTTAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGATTc                                                                                                                                                      <  1:87770/151‑1 (MQ=255)      cTGCCGTACAGGCAGCTTAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGGTTCGAAcc                                                                                                                                                 >  2:816673/1‑151 (MQ=255)          cGTACAGGCAGCTTAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGGTTTGAAccccc                                                                                                                                              >  2:224521/1‑150 (MQ=255)          cGTACAGGCAGCTTAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGAG‑AGGGGGGGGGTCGACcacccc                                                                                                                                             >  1:667320/1‑150 (MQ=255)           gTACAGGCAGCTTAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGAA‑AGGGGGGGGTTTGacaccc                                                                                                                                               >  1:934186/1‑143 (MQ=38)             aCAGGCAGCTTAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGATTCGAACCCCCGAt                                                                                                                                           <  1:926644/150‑1 (MQ=255)              cAGGCAGCTTAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGGGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGATTCGAACCCCCGATAc                                                                                                                                         <  2:394341/151‑1 (MQ=255)               aGGCAGCTTAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑GGGGGGGGGTTTGAAccccc                                                                                                                                              >  1:36190/1‑145 (MQ=255)                         gAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGATTCGAACCCCCGATACGTTGCCGtata                                                                                                                              <  1:528540/151‑1 (MQ=255)                           aaTTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGATTCGAACCCCCGATACGTTGCCGTATaca                                                                                                                            <  1:525145/151‑1 (MQ=255)                                 caGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACttt                                                                                                                      <  1:960836/151‑1 (MQ=255)                                      aaCATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAg                                                                                                                  <  1:74217/150‑1 (MQ=255)                                             tCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGCG‑AGGGGGGGGTTCGGAACCCCCCGAAGGTGCCGTATAAAAACTTTCCAGGGGt                                                                                                              >  1:224252/1‑147 (MQ=21)                                               cACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGGTTCTAACCCCCCACACGTTGCCGGATTCAAACATTTCAgg                                                                                                                 >  2:1552/1‑142 (MQ=21)                                                          caTGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGGGTCGGACCCCCCCTACGTTGGCGTATACACACCTTTCCCGCGGGGTCCTTCAG‑cccccc                                                                                             >  1:39280/1‑146 (MQ=18)                                                            tGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGGTTCTAACCCCCCACACGTTGCCGTATAAACACTTTCCAGGCGGGCTCCCTCCG‑ccccccg                                                                                            >  1:146786/1‑144 (MQ=18)                                                                     ccGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGGTTTGAAACCCCCATACGTTGCCGTATACACACTTTCCAGGCGGGCTCCTTCCG‑CCCCTCCGGCACCCCA‑cc                                                                                >  2:778577/1‑151 (MQ=17)                                                                           agacagaTAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGGTTCTAACCCCCCATACGTTTCCGGATACACACTTTTCCCGCGGGCTCCTTCCG‑CCCCCCGGGCACCCCACCCAAAtt                                                                           >  2:755609/1‑151 (MQ=14)                                                                            gacagaTAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGGGTTGAAACCCCCAAAACTTTCCCTATACACACCTTTCAGGGGGGGTCCCTCCA‑CCCCCCCGGCACCCCA‑CCaaa                                                                             >  1:55591/1‑147 (MQ=9)                                                                            gacagaTAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAG‑CCACTCGGACACCTCA‑CCa                                                                               <  2:55623/145‑1 (MQ=255)                                                                               agaTAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGAGAAGGGGGGGGTTC‑AACCCCCCCAACGTTGCCATTTAAAACCTTTCCAGGCGTGGGCCCTTCG‑CCACCCGGACACCCCC‑CCAAAATGtttt                                                                      >  1:921516/1‑151 (MQ=9)                                                                                      aTGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAG‑CCACTCGGACACCTCA‑CCAAATTGTTTTGCTACCa                                                               <  2:588666/151‑1 (MQ=255)                                                                                          gAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGGGTTGAAACCCCGATACGTTTCCGTATAAACACTTTTCCGGGGGGGTCCTTCAG‑CCACTCCGCCACCCCA‑CCAAATTTTTTTGCTACCcaaac                                                           >  2:163933/1‑149 (MQ=14)                                                                                              aaaaaGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGAT‑AGGGGGGGGGTCGAACCCCCGATACGGTTTCGTATAAACACTTTTCAGGCGTGGTCCTTTCG‑CCACTCCGGCACCCCA‑CCAAAAAATTTTTCTAACaaaac                                                           >  1:114537/1‑145 (MQ=2)                                                                                               aaaaGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAG‑CCACTCGGACACCTCA‑CCAAATTGTTTTGCTACCAAACCTCATg                                                      <  2:872662/151‑1 (MQ=255)                                                                                                          cTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGGTTCGAACCCCCCCTACGTTTTCCTATAAACAATTTTCCGGCGTGCTCCCTCAGCCCACCCGG‑CACCTCA‑CCCAAATTTTTTTCTACCAAAACCCCTGGGGGGGaa                                              >  1:155018/1‑148 (MQ=2)                                                                                                               gTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAG‑CCACTCGGACACCTCA‑CCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGgcgc                                      <  2:867912/151‑1 (MQ=255)                                                                                                                tACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGGTTTGGAACCCCGATACCTTTCAGTATACAAACCTTTCAGGCGTGGTCCTTCCG‑CCCCCCGGACACCTCA‑CCAAATTATTTTTCTTCCAAAACTCATTGGTGGGTACGGGgc                                        >  1:214132/1‑148 (MQ=9)                                                                                                                 aCGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAG‑CCACTCGGACACCTCA‑CCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGgcgc                                      <  1:173452/149‑1 (MQ=255)                                                                                                                  cGAGCTCTTCGTTAAATATGGCGGTG‑AGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCGGGCGTGCTCCTTCAG‑CCACTCGGACACCTCA‑CCAAATTGTTTTGCTACCAAGCCTCATGGGTGGCAACGGGGCGcta                                    <  1:726273/150‑1 (MQ=255)                                                                                                                     gCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAG‑CCACTCGGACACCTCA‑CCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGctacta                                 <  2:246333/150‑1 (MQ=255)                                                                                                                               aaaTATGGCGGTG‑AGGGGGGGGATCGAAACCCCCATAAGGTGCCGTATAAACACTTTCCCGGGGGGCGCCTTCAC‑CCCATCGGGCACCCCA‑ACAAAAAGTTTTGCTACCAAACCGCCTGGGAGGCAACCGGgagc                                      >  1:280966/1‑135 (MQ=9)                                                                                                                               aaaTATGGCGGTG‑AGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAG‑CCACTCGGACACCTCA‑CCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGTGCGCTACTATAGGGAGTTgg                      <  1:614421/151‑1 (MQ=255)                                                                                                                               aaaTATGGCGGTG‑AGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAG‑CCACTCGGACACCTCA‑CCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTg                       <  1:399881/150‑1 (MQ=255)                                                                                                                                aaTATGGCGGTG‑AGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAG‑CCACTCGGACACCTCA‑CCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGa                     <  2:162419/151‑1 (MQ=255)                                                                                                                                aaTATGGCGGTG‑AGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAG‑CCACTCGGACACCTCA‑CCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGa                     <  2:862976/151‑1 (MQ=255)                                                                                                                                   atGGCGGTG‑AGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCCA‑CCACTCGGCCACCCCA‑CCCAATTGTTTTGCTACCAAACCTCATGGGTGGCAACCGGGCGCTACTATAGGGAGTTGGAg                    >  1:128391/1‑149 (MQ=21)                                                                                                                                     ggCGGTG‑AGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAG‑CCACTCGGACACCTCA‑CCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTaa                 >  1:950658/1‑150 (MQ=255)                                                                                                                                           g‑AGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAG‑CCACTCGGACACCTCA‑CCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTc          <  2:682205/151‑1 (MQ=255)                                                                                                                                             aGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAG‑CCACTCGGACACCTCA‑CCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTc          >  1:670914/1‑150 (MQ=255)                                                                                                                                               ggggggATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAG‑CCACTCGGACACCTCA‑CTAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTCaag       <  1:300322/151‑1 (MQ=255)                                                                                                                                                 ggggATTCGAACCCCCGAATTGTTGCCCAACGCAGACTTTCCAGGCGTGCTCCTTCAG‑ACAGTCAGAACCCTCA‑ACAAAGTGTTTGGCTACCAAACCTCACGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTCaag       <  1:563677/149‑1 (MQ=25)                                                                                                                                                  gggATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAG‑CCACTCGGACACCTCA‑CCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTCaagaag    <  1:317943/151‑1 (MQ=255)                                                                                                                                                    gATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAG‑CCACTCGGACACCTCA‑CCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTCAAgaagaa  <  2:161175/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                        GTTCACTGCCGTACAGGCAGCTTAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTG‑AGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAG‑CCACTCGGACACCTCA‑CCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTCAAGAAGAA  >  NC_000913/925753‑926049

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑