Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	824,028	A→G	L157P (CTC→CCC)	ybhP ←	endo/exonuclease/phosphatase family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	824,028	0	A	G	100.0%	217.3 / NA	56	L157P (CTC→CCC)	ybhP	endo/exonuclease/phosphatase family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/27);  total (29/27)

CGCGCCGGGCGTCCGTGGGCGCGGGTAAAAATCTCATCCAGTCCGGCCTGCACTTTTAACGGATGATTAGCTTTTTGCCGCCAGTCATTGAAATCACCCGCCACCAATACCGGTTCGCCGTCCGGTAGCTCATTCACCCATTCGGCGAGCATCGCAAGCTGCGCCTGACGGTGCGCCTCACGCAGGCCCAGATGTACGCACATCACATGAATCGCTTTTCCGGTCATCGGCGGCACAATGCGGCAGTAGAGCACGCCGCGCTTTTCCGCACCATCGACCGAAACATCGCGA  >  NC_000913/823881‑824171                                                                                                                                                    |                                                                                                                                                cgcgcCGGGCGTCCGTGGGCGCGGGTAAAAATCTCATCCAGTCCGGCCTGCACTTTTAACGGATGATTAGCTTTTTGCCGCCAGTCATTGAAATCACCCGCCACCAATACCGGTTCGCCGTCCGGTAGCTCATTCACCCATTCGGCGGGc                                                                                                                                               >  1:386265/1‑150 (MQ=255)  gtgcCGGGCGTCCGTGGGAGCGGGTAAAACTCTCATCCAGTCCGGCCTGCCCTTTTAACGGATGATTAGCTTTTTGCCCCCAGTCCTTGAAATCACCCGCCACCAATACCGGTTCGCCGTCCGGTAGCTCATTCACCCATTCGGCGGGCAt                                                                                                                                             <  1:483450/149‑1 (MQ=255)   cgcCGGGCGTCCGTGGGCGCGGGTAAAAATCTCATCCAGTCCGGCCTGCACTTTTAACGGATGATTAGCTTTTTGCCGCCAGTCATTGAAATCACCCGCCACCAATACCGGTTCGCCGTCCGGTAGCTCATTCACCCATTCGGCGGGCATc                                                                                                                                            >  1:374952/1‑151 (MQ=255)    gcCGGGCGTCCGTGGGCGCGGGTAAAAATCTCATCCAGTCCGGCCTGCCCTTTTAACGGATGATTAGCTTTTTGCCGCCAGTCATTGAAATCACCCGCCACCAATACCGGTTCGCCGTCCGGTAGCTCATTCACCCATTCGGCGGGCATc                                                                                                                                            <  1:397245/150‑1 (MQ=255)               gTGGGCGCGGGTAAAAATCTCATCCAGTCCGGCCTGCACTTTTAACGGATGATTAGCTTTTTGCCGCCAGTCATTGAAATCACCCGCCACCAATACCGGTTCGCCGTCCGGTAGCTCATTCACCCATTCGGCGGGCATCGCAAGCTGCGcc                                                                                                                                >  2:423858/1‑151 (MQ=255)               gTGGGCGCGGGTAAAAATCTCATCCAGTCCGGCCTGCACTTTTAACGGATGATTAGCTTTTTGCCGCCAGTCATTGAAATCACCCGCCACCAATACCGGTTCGCCGTCCGGTAGCTCATTCACCCATTCGGCGGGCATCGCAAGCTGCGcc                                                                                                                                >  2:316134/1‑151 (MQ=255)                   gcgcGGGTAAAAATCTCATCCAGTCCGGCCTGCACTTTTAACGGATGATTAGCTTTTTGCCGCCAGTCATTGAAATCACCCGCCACCAATACCGGTTCGCCGTCCGGTAGCTCATTCACCCATTCGGCGGGCATCGCAAGCTGCGCCTGAc                                                                                                                            <  2:537297/151‑1 (MQ=255)                        ggTAAAAATCTCATCCAGTCCGGCCTGCACTTTTAACGGATGATTAGCTTTTTGCCGCCAGTCATTGAAATCACCCGCCACCAATACCGGTTCGCCGTCCGGTAGCTCATTCACCCATTCGGCGGGCATCGCAAGCTGCGCCTGACGGTgc                                                                                                                       >  1:89341/1‑151 (MQ=255)                                       cAGTCCGGCCTGCACTTTTAACGGATGATTAGCTTTTTGCCGCCAGTCATTGAAATCACCCGCCACCAATACCGGTTCGCCGTCCGGTAGCTCATTCACCCATTCGGCGGGCATCGCAAGCTGCGCCTGACGGTGCGCCTCACGCAGGccc                                                                                                        >  2:841112/1‑151 (MQ=255)                                           ccGGCCTGCACTTTTAACGGATGATTAGCTTTTTGCCGCCAGTCATTGAAATCACCCGCCACCAATACCGGTTCGCCGTCCGGTAGCTCATTCACCCATTCGGCGGGCATCGCAAGCTGCGCCTGACGGTGCGCCTCACGCAGGCCCAGa                                                                                                     <  1:266651/150‑1 (MQ=255)                                            cGGCCTGCACTTTTAACGGATGATTAGCTTTTTGCCGCCAGTCATTGAAATCCCCCGCCACCAATACCGGTTCGCCGTCCGGTAGCTCATTCACCCATTCGGCGGGCATCGCAAGCTGCGCCTGACGGTGCGCCTCACGCAGGCCCAGATg                                                                                                   <  1:823134/151‑1 (MQ=255)                                            cGGCCTGCACTTTTAACGGATGATTAGCTTTTTGCCGCCAGTCATTGAAATCACCCGCCACCAATACCGGTTCGCCGTCCGGTAGCTCATTCACCCATTCGGCGGGCATCGCAAGCTGCGCCTGACGGTGCGCCTCACGCAGGCCCAGATg                                                                                                   >  2:449049/1‑151 (MQ=255)                                                cTGCACTTTTAACGGATGATTAGCTTTTTGCCGCCAGTCATTGAAATCACCCGCCACCAATACCGGTTCGCCGTCCGGTAGCTCATTCACCCATTCGGCGGGCATCGCAAGCTGCGCCTGACGGTGCGCCTCACGCAGGCCCAGATGTACg                                                                                               >  2:306564/1‑151 (MQ=255)                                                cTGCACTTTTAACGGATGATTAGCTTTTTGCCGCCAGTCATTGAAATCACCCGCCACCAATACCGGTTCGCCGTCCGGTAGCTCATTCACCCATTCGGCGGGCATCGCAAGCTGCGCCTGACGGTGCGCCTCACGCAGGCCCAGATGTACg                                                                                               >  2:521062/1‑151 (MQ=255)                                                 tGCACTTTTAACGGATGATTAGCTTTTTGCCGCCAGTCATTGAAATCACCCGCCACCAATACCGGTTCGCCGTCCGGTAGCTCATTCACCCATTCGGCGGGCATCGCAAGCTGCGCCTGACGGTGCGCCTCACGCAGGCCCAGATGTACGc                                                                                              >  1:18996/1‑151 (MQ=255)                                                      ttttAACGGATGATTAGCTTTTTGCCGCCAGTCATTGAAATCACCCGCCACCAATACCGGTTCGCCGTCCGGTAGCTCATTCACCCATTCGGCGGGCATCGCAAGCTGCGCCTGACGGTGCGCCTCACGCAGGCCCAGATGTACGcacatc                                                                                         >  1:93845/1‑151 (MQ=255)                                                        ttAACGGATGATTAGCTTTTTGCCGCCAGTCATTGAAATCACCCGCCACCAATACCGGTTCGCCGTCCGGTAGCTCATTCACCCATTCGGCGGGCATCGCAAGCTGCGCCTGACGGTGCGCCTCACGCAGGCCCAGATGTACGcacatca                                                                                        <  2:662352/150‑1 (MQ=255)                                                         tAACGGATGATTAGCTTTTTGCCGCCAGTCATTGAAATCACCCGCCACCAATACCGGTTCGCCGTCCGGTAGCTCATTCACCCATTCGGCGGGCATCGCAAGCTGCGCCTGACGGTGCGCCTCACGCAGGCCCAGATGTACGcacatcac                                                                                       >  2:318547/1‑150 (MQ=255)                                                            cGGATGATTAGCTTTTTGCCGCCAGTCATTGAAATCACCCGCCACCAATACCGGTTCGCCGTCCGGTAGCTCATTCACCCATTCGGCGGGCATCGCAAGCTGCGCCTGACGGTGCGCCTCACGCAGGCCCAGATGTACGcacatcacat                                                                                     >  1:672348/1‑149 (MQ=255)                                                               atgatTAGCTTTTTGCCTCCAGTCATTGAAATCTCCCGCCACCAATACCGGTTCGCCGTCCGGTAGCTCATTCACCCATTCGGCGGGCATCGCAAGCTGCGCCTGACGGTGCGCCTCACGCAGGCCCAGATGTACGCACATCACATGaa                                                                                  <  1:773865/149‑1 (MQ=255)                                                               atgatTAGCTTTTTGCCGCCAGTCATTGAAATCACCCGCCACCAATACCGGTTCGCCGTCCGGTAGCTCATTCACCCATTCGGCGGGCATCGCAAGCTGCGCCTGACGGTGCGCCTCACGCAGGCCCAGATGTACGCACATCACATGAATc                                                                                <  2:319261/151‑1 (MQ=255)                                                                  atTAGCTTTTTGCCGCCAGTCATTGAAATCACCCGCCACCAATACCGGTTCGCCGTCCGGTAGCTCATTCACCCATTCGGCGGGCATCGCAAGCTGCGCCTGACGGTGCGCCTCACGCAGGCCCAGATGTACGCACATCACATGAATCGCt                                                                             >  2:628953/1‑151 (MQ=255)                                                                  atTAGCTTTTTGCCGCCAGTCAATGAAATCCCCCGCCACCAATACCGGTTCGCCGTCCGGTAGCTCATTCACCCATTCGGCGGGCATCGCAAGCTGCGCCTGACGGTGCGCCTCACGCAGGCCCAGATGTACGCACATCACATGAATc                                                                                <  2:442063/148‑1 (MQ=255)                                                                     aGCTTTTTGCCGCCAGTCATTGAAATCACCCGCCACCAATACCGGTTCGCCGTCCGGTAGCTCATTCACCCATTCGGCGGGCATCGCAAGCTGCGCCTGACGGTGCGCCTCACGCAGGCCCAGATGTACGCACATCACATGAATCGCtttt                                                                          >  1:510086/1‑151 (MQ=255)                                                                      gCTTTTTGCCGCCAGTCATTGAAATCACCCGCCACCAATACCGGTTCGCCGTCCGGTAGCTCATTCACCCATTCGGCGGGCATCGCAAGCTGCGCCTGACGGTGCGCCTCACGCAGGCCCAGATGTACGCACATCACATGAATCGCTTTTc                                                                         >  2:310749/1‑151 (MQ=255)                                                                           ttGCCGCCAGTCATTGAAATCACCCGCCACCAATACCGGTTCGCCGTCCGGTAGCTCATTCACCCATTCGGCGGGCATCGCAAGCTGCGCCTGACGGTGCGCCTCACGCAGGCCCAGATGTACGCACATCACATGAATCGCTTTTCCGGTc                                                                    >  2:6028/1‑151 (MQ=255)                                                                               cgccAGTCATTGAAATCACCCGCCACCAATACCGGTTCGCCGTCCGGTAGCTCATTCACCCATTCGGCGGGCATCGCAAGCTGCGCCTGACGGTGCGCCTCACGCAGGCCCAGATGTACGCACATCACATGAATCGCTTTTCCGGTCATcg                                                                >  1:145679/1‑151 (MQ=255)                                                                                 ccactaaTTGAAATCACCCGCCACCAATACCGGTTCGCCGTCCGGTAGCTCATTCACCCATTCGGCGGGCATCGCAAGCTGCGCCTGACGGTGCGCCTCACGCAGGCCCAGATGTACGCACATCACATGAATCGCTTTTCCGGTc                                                                    <  1:88094/139‑1 (MQ=255)                                                                                  cAGTCATTGAAATCACCCGCCACCAATACCGGTTCGCCGTCCGGTAGCTCATTCACCCATTCGGCGGGCATCGCAAGCTGCGCCTGACGGTGCGCCTCACGCAGGCCCAGATGTACGCACATCACATGAATCGCTTTTCCGGTCATcgg                                                               <  1:210649/149‑1 (MQ=255)                                                                                    gTCATTGAAATCACCCGCCACCAATACCGGTTCGCCGTCCGGTAGCTCATTCACCCATTCGGCGGGCATCGCAAGCTGCGCCTGACGGTGCGCCTCACGCAGGCCCAGATGTACGCACATCACATGAATCGCTTTTCCGGTCATCggcggc                                                           >  2:225782/1‑151 (MQ=255)                                                                                     tCATTGAAATCACCCGCCACCAATACCGGTTCGCCGTCCGGTAGCTCATTCACCCATTCGGCGGGCATCGCAAGCTGCGCCTGACGGTGCGCCTCACGCAGGCCCAGATGTACGCACATCACATGAATCGCTTTTCCGGTCATCggcggc                                                           <  2:710072/150‑1 (MQ=255)                                                                                        ttGAAATCACCCGCCACCAATACCGGTTCGCCGTCCGGTAGCTCATTCACCCATTCGGCGGGCATCGCAAGCTGCGCCTGACGGTGCGCCTCACGCAGGCCCAGATGTACGCACATCACATGAATCGCTTTTCCGGTCATCGGCGGcaca                                                        >  1:890353/1‑150 (MQ=255)                                                                                         tGAAATCACCCGCCACCAATACCGGTTCGCCGTCCGGTAGCTCATTCACCCATTCGGCGGGCATCGCAAGCTGCGCCTGACGGTGCGCCTCACGCAGGCCCAGATGTACGCACATCACATGAATCGCTTTTCCGGTCATCGGCGGCACaa                                                       <  2:804330/150‑1 (MQ=255)                                                                                            aaTCACCCGCCACCAATACCGGTTCGCCGTCCGGTAGCTCATTCACCCATTCGGCGGGCATCGCAAGCTGCGCCTGACGGTGCGCCTCACGCAGGCCCAGAAGTACGCACATCACATGAATCGCTTTTCCGGTCATCGGCGGCACCATGc                                                    >  1:515927/1‑150 (MQ=255)                                                                                               cccccGCCACCAATACCGGTTCGCCGTCCGGTAGCTCATTCACCCATTCGGCGGGCATCGCAAGCTGCGCCTGACGGTGCGCCTCACGCAGGCCCAGATGTACGCACATCACATGAATCGCTTTTCCGGTCATCGGCGGCACAATGCGGc                                                 <  2:969921/148‑1 (MQ=255)                                                                                                   cGCCACCAATACCGGTTCGCCGTCCGGTAGCTCATTCACCCATTCGGCGGGCATCGCAAGCTGCGCCTGACGGTGCGCCTCACGCAGGCCCAGATGTACGCACATCACATGAATCGCTTTTCCGGTCATCGGCGGCACAATGCGGCAGt                                              >  2:39424/1‑149 (MQ=255)                                                                                                   cGCCACCAATACCGGTTCGCCGTCCGGTAGCTCATTCACCCATTCGGCGGGCATCGCAAGCTGCGCCTGACGGTGCGCCTCACGCAGGCCCAGATGTACGCACATCACATGAATCGCTTTTCCGGTCATCGGCGGCACAATGCGGCAGTag                                            <  2:372140/151‑1 (MQ=255)                                                                                                   cGCCACCAATACCGGTTCGCCGTCCGGTAGCTCATTCACCCATTCGGCGGGCATCGCAAGCTGCGCCTGACGGTGCGCCTCACGCAGGCCCAGATGTACGCACATCACATGAATCGCTTTTCCGGTCATCGGCGGCACAATGCGGCAGTag                                            <  2:127729/151‑1 (MQ=255)                                                                                                    gCCACCAATACCGGTTCGCCGTCCGGTAGCTCATTCACCCATTCGGCGGGCATCGCAAGCTGCGCCTGACGGTGCGCCTCACGCAGGCCCAGATGTACGCACATCACATGAATCGCTTTTCCGGTCATCGGCGGCACAATGCGGCAGTaga                                           <  1:402768/151‑1 (MQ=255)                                                                                                       accaATACCGGTTCGCCGTCCGGTAGCTCATTCACCCATTCGGCGGGCATCGCAAGATGCGCCTGACGGTGCGCCTCACGCAGGCCCCGATGGACGCACATCACATGAATCGCTTTTCCGGTCCTCGGCGGCACAATGCGGCAGGagcg                                          >  1:87549/1‑147 (MQ=255)                                                                                                           aTACCGGTTCGCCGTCCGGTAGCTCATTCACCCATTCGGCGGGCATCGCAAGCTGCGCCTGACGGTGCGCCTCACGCAGGCCCAGATGTACGCACATCACATGAATCGCTTTTCCGGTCATCGGCGGCACAATGCGGCAGTAGAGCAcgcc                                    >  1:311180/1‑151 (MQ=255)                                                                                                             aCCGGTTCGCCGTCCGGTAGCTCATTCACCCATTCGGCGGGCATCGCAAGCTGCGCCTGACGGTGCGCCTCACGCAGGCCCAGATGTACGCACATCACATGAATCGCTTTTCCGGTCATCGGCGGCACAATGCGGCAGTAGAGCAcgccg                                   <  1:362725/150‑1 (MQ=255)                                                                                                             aCCGGTTCGCCGTCCGGTAGCTCATTCACCCATTCGGCGGGCATCGCAAGCTGCGCCTGACGGTGCGCCTCACGCAGGCCCAGATGTACGCACATCACATGAATCGCTTTTCCGGTCATCGGCGGCACAATGCGGCAGTAGAGCAcgccg                                   <  1:124734/150‑1 (MQ=255)                                                                                                             aCCGGTTCGCCGTCCGGTAGCTCATTCACCCATTCGGCGGGCATCGCAAGCTGCGCCTGACGGTGCGCCTCACGCAGGCCCAGATGTACGCACATCACATGAATCGCTTTTCCGGTCATCGGCGGCACAATGCGGCAGTAGAGCAcgcc                                    >  1:260568/1‑149 (MQ=255)                                                                                                                    cGCCGTCCGGTAGCTCATTCACCCATTCGGCGGGCATCGCAAGCTGCGCCTGACGGTGCGCCTCACGCAGGCCCAGATGTACGCACATCACATGAATCGCTTTTCCGGTCATCGGCGGCACAATGCGGCAGTAGAGCACGCCGCGCTTTTc                           >  2:778867/1‑151 (MQ=255)                                                                                                                     gCCGTCCGGTAGCTCATTCACCCATTCGGCGGGCATCGCAAGCTGCGCCTGACGGTGCGCCTCACGCAGGCCCAGATGTACGCACATCACATGAATCGCTTTTCCGGTCATCGGCGGCACAATGCGGCAGTAGAGCACGCCGCGCTTTTcc                          >  1:426869/1‑151 (MQ=255)                                                                                                                     gCCGTCCGGTAGCTCATTCACCCATTCGGCGGGCATCGCAAGCTGCGCCTGACGGTGCGCCTCACGCAGGCCCAGATGTACGCACATCACATGAATCGCTTTTCCGGTCATCGGCGGCACAATGCGGCAGTAGAGCACGCCGCGCTTTTcc                          >  2:317745/1‑151 (MQ=255)                                                                                                                        gTCCGGTAGCTCATTCACCCATTCGGCGGGCATCGCAAGCTGCGCCTGACGGTGCGCCTCACGCAGGCCCAGATGTACGCACATCACATGAATCGCTTTTCCGGTCATCGGCGGCACAATGCGGCAGTAGAGCACGCCGCGCTTTTCCGc                        <  1:544422/150‑1 (MQ=255)                                                                                                                             gTAGCTCATTCACCCATTCGGCGGGCATCGCAAGCTGCGCCTGACGGTGCGCCTCACGCAGGCCCAGATGTACGCACATCACATGAATCGCTTTTCCGGTCATCGGCGGCACAATGCGGCAGTAGAGCACGCCGCGCTTTTCCGCACCATc                  <  1:92619/151‑1 (MQ=255)                                                                                                                             gTAGCTCATTCACCCATTCGGCGGGCATCGCAAGCTGCGCCTGACGGTGCGCCTCACGCAGGCCCAGATGTACGCACATCACATGAATCGCTTTTCCGGTCATCGGCGGCACAATGCGGCAGTAGAGCACGCCGCGCTTTTCCGCACCATc                  <  2:352352/151‑1 (MQ=255)                                                                                                                                       cACCCATTCGGCGGGCATCGCAAGCTGCGCCTGACGGTGCGCCTCACGCAGGCCCAGATGTACGCACATCACATGAATCGCTTTTCCGGTCATCGGCGGCACAATGCGGCAGTAGAGCACGCCGCGCTTTTCCGCACCATCGACCGAAACa        <  2:697740/151‑1 (MQ=255)                                                                                                                                         cccATTCGGCGGGCATCGCAAGCTGCGCCTGACGGTGCGCCTCACGCAGGCCCAGATGTACGCACATCACATGAATCGCTTTTCCGGTCATCGGCGGCACAATGCGGCAGTAGAGCACGCCGCGCTTTTCCGCACCATCGACCGAAACa        <  2:201729/149‑1 (MQ=255)                                                                                                                                         cccATTCGGCGGGCATCGCAAGCTGCGCCTGACGGTGCGCCTCACGCAGGCCCAGATGTACGCACATCACATGAATCGCTTTTCCGGTCATCGGCGGCACAATGCGGCAGTAGAGCACGCCGCGCTTTTCCGCACCATCGACCGAAACATc      <  1:271059/151‑1 (MQ=255)                                                                                                                                            aTTCGGCGGGCATCGCAAGCTGCGCCTGACGGTGCGCCTCACGCAGGCCCAGATGTACGCACATCACATGAATCGCTTTTCCGGTCATCGGCGGCACAATGCGGCAGTAGAGCACGCCGCGCTTTTCCGCACCATCGACCGAAACATcgcg   >  2:890632/1‑151 (MQ=255)                                                                                                                                              tCGGCGGGCATCGCAAGCTGCGCCTGACGGTGCGCCTCACGCAGGCCCAGATGTACGCACATCACATGAATCGCTTTTCCGGTCATCGGCGGCACAATGCGGCAGTAGAGCACGCCGCGCTTTTCCGCACCATCGACCGAAACATcgcg   <  1:457896/149‑1 (MQ=255)                                                                                                                                              tCGGCGGGCATCGCAAGCTGCGCCTGACGGTGCGCCTCACGCAGGCCCAGATGTACGCACATCACATGAATCGCTTTTCCGGTCATCGGCGGCACAATGCGGCAGTAGAGCACGCCGCGCTTTTCCGCACCATCGACCGAAACATCGCGa  <  2:480137/150‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                CGCGCCGGGCGTCCGTGGGCGCGGGTAAAAATCTCATCCAGTCCGGCCTGCACTTTTAACGGATGATTAGCTTTTTGCCGCCAGTCATTGAAATCACCCGCCACCAATACCGGTTCGCCGTCCGGTAGCTCATTCACCCATTCGGCGAGCATCGCAAGCTGCGCCTGACGGTGCGCCTCACGCAGGCCCAGATGTACGCACATCACATGAATCGCTTTTCCGGTCATCGGCGGCACAATGCGGCAGTAGAGCACGCCGCGCTTTTCCGCACCATCGACCGAAACATCGCGA  >  NC_000913/823881‑824171

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑