Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,722,386 C→T T89M (ACG→ATG)  ydhK → putative efflux protein (PET) component of YdhJK efflux pump

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,722,3860CT98.8% 312.5 / ‑5.0 80T89M (ACG→ATG) ydhKputative efflux protein (PET) component of YdhJK efflux pump
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (38/41);  minor base G (0/1);  total (38/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

TTTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCTTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGCCGGGCATACGCTCAATGAGCCGTGGTTTTTTCTATTGAGCATGTCGGCGTGGCTTGGCTTTTGTACCTGGGCCTGTGCGCACTTCACGAATAACGTCGCGTATGCATTTCAACTGGCGGGCTACACGGCTGCCATCATCGCCTTTCCGATGGTTA  >  NC_000913/1722240‑1722532
                                                                                                                                                  |                                                                                                                                                  
tttAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCTTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTGCTTGCCGGGCATATGCt                                                                                                                                                 <  1:327557/150‑1 (MQ=255)
    aaTCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCTTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGCCGGGCATATGCTCAATg                                                                                                                                            >  1:141928/1‑151 (MQ=255)
    aaTCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCTTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGCCGGGCATATGCTCAATg                                                                                                                                            >  2:412965/1‑151 (MQ=255)
        tGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCTTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGCCGGGCATATGCTCAATGAGcc                                                                                                                                        >  2:112630/1‑151 (MQ=255)
         ggATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCTTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGCCGGGCATATGCTCAATGAGCCg                                                                                                                                       >  2:422657/1‑151 (MQ=255)
          gATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCTTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGCCGGGCATATGCTCAATGAGCCGt                                                                                                                                      <  2:613612/151‑1 (MQ=255)
                  cTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCTTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGCCGGGCATATGCTCAATGAGCCGTGGtttttt                                                                                                                              >  2:346973/1‑151 (MQ=255)
                  cTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCTTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGCCGGGCATATGCTCAATGAGCCGTGGtttttt                                                                                                                              >  2:296438/1‑151 (MQ=255)
                     ttGGGCGATGACCTCGGCTGCAGTGGTTAGCTTTCCCGCCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGCCGGGCATATGCTCAATGAGCCGTGGtttttt                                                                                                                              <  1:322692/148‑1 (MQ=255)
                      tGGGCGATGACCTCGGCTGCAGTGGTTAGCTTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGCCGGGCATATGCTCAATGAGCCGTGGTTTTTTCTAt                                                                                                                          >  1:968403/1‑151 (MQ=255)
                       gggCGATGACCTCGGCTGCAGTGGTTAGCTTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGCCGGGCATATGCTCAATGAGCCGTGGTTTTTTCTAtt                                                                                                                         <  2:196663/151‑1 (MQ=255)
                       gggCGATGACCTCGGCTACAGTGGTTAGCTTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGCCGGGCATATGCTCAATGAGCCGTGGTTTTTTCTAt                                                                                                                          <  2:412704/150‑1 (MQ=255)
                        ggCGATGACCTCGGCTGCAGTGGTTAGCTTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGCCGGGCATATGCTCAATGAGCCGTGGTTTTTTCTATTg                                                                                                                        <  1:921575/151‑1 (MQ=255)
                          cGATGACCTCGGCTGCAGTGGTTAGCTTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGCCGGGCATATGCTCAATGAGCCGTGGTTTTTTCTATTg                                                                                                                        >  2:543537/1‑149 (MQ=255)
                          agATGACCTCGGCTGCAGTGGTTAGCTTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGCCGGGCATATGCTCAATGAGCCGTGGTTTTTTCTATTg                                                                                                                        >  1:862608/2‑149 (MQ=255)
                                ccTCGGCTGCAGTGGTTAGCTTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGCCGGGCATATGCTCAATGAGCCGTGGTTTTTTCTATTGAGCATGTc                                                                                                                <  1:203815/151‑1 (MQ=255)
                                   cGGCTGCAGTGGTTAGCTTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGCCGGGCATATGCTCAATGAGCCGTGGTTTTTTCTATTGAGCATGTCg                                                                                                               <  2:112413/149‑1 (MQ=255)
                                    ggCTGCAGTGGTTAGCTTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGCCGGGCATATGCTCAATGAGCCGTGGTTTTTTCTATTGAGCATGTCGGCg                                                                                                            <  2:784605/151‑1 (MQ=255)
                                        gCAGTGGTTAGCTTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGCCGGGCATATGCTCAATGAGCCGTGGTTTTTTCTATTGAGCATGTCGGCGtggc                                                                                                        >  2:305016/1‑151 (MQ=255)
                                        gCAGTGGTTAGCTTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGCCGGGCATATGCTCAATGAGCCGTGGTTTTTTCTATTGAGCATGTCGGCGtg                                                                                                          <  2:968502/149‑1 (MQ=255)
                                         cAGTGGTTAGCTTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGCCGGGCATATGCTCAATGAGCCGTGGTTTTTTCTATTGAGCATGTCGGCGtggct                                                                                                       <  2:8978/151‑1 (MQ=255)
                                              gTTAGCTTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGCCGGGCATATGCTCAATGAGCCGTGGTTTTTTCTATTGAGCATGTCGGCGtggcttggct                                                                                                  <  2:702712/151‑1 (MQ=255)
                                                tAGCTTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCCGGGAGCCATTGCGGCACTGCTTCTTGCCGGGCATATGCTCAATGAGCCGTGGTTTTTTCTATTGAGCATGTCGGCGTggcttggctt                                                                                                 <  1:83850/150‑1 (MQ=255)
                                                   cTTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGCCGGGCATATGCTCAATGAGCCGTGGTTTTTTCTATTGAGCATGTCGGCGTGGCTTGGCTTTTGt                                                                                             >  2:639109/1‑151 (MQ=255)
                                                    tttCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGCCGGGCATATGCTCAATGAGCCGTGGTTTTTTCTATTGAGCATGTCGGCGTGGCTTGGCTTTTGt                                                                                             >  2:106245/1‑150 (MQ=255)
                                                     ttCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGCCGGGCATATGCTCAATGAGCCGTGGTTTTTTCTATTGAGCATGTCGGCGTGGCTTGGCTTTTGt                                                                                             <  2:366539/149‑1 (MQ=255)
                                                      tCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGCCGGGCATATGCTCAATGAGCCGTGGTTTTTTCTATTGAGCATGTCGGCGTGGCTTGGCTTTTGTAc                                                                                           <  1:637989/150‑1 (MQ=255)
                                                        ccACCGTTGGCGGTGTTATCAGCAAAAGCCTCGTACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGCCGGGCATATGCTCAATGAGCCGTGGTTTTTTCTATTGAGCATGTCGGCGTGGCTTGGCTTTTGTACCt                                                                                         <  1:454698/150‑1 (MQ=255)
                                                         cACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGCCGGGCATATGCTCAATGAGCCGTGGTTTTTTCTATTGAGCATGTCGGCGTGGCTTGGCTTTTGTACCTgg                                                                                       >  2:646994/1‑151 (MQ=255)
                                                         cACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGCCGGGCATATGCTCAATGAGCCGTGGTTTTTTCTATTGAGCATGTCGGCGTGGCTTGGCTTTTGTACCTgg                                                                                       <  1:233291/151‑1 (MQ=255)
                                                           ccGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGCCGGGCATATGCTCAATGAGCCGTGGTTTTTTCTATTGAGCATGTCGGCGTGGCTTGGCTTTTGTACCTGGGc                                                                                     <  1:399980/151‑1 (MQ=255)
                                                             gTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGCCGGGCATATGCTCAATGAGCCGTGGTTTTTTCTATTGAGCATGTCGGCGTGGCTTGGCTTTTGTACCTGGGCCt                                                                                   >  2:825665/1‑151 (MQ=255)
                                                              ttGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGCCGGGCATATGCTCAATGAGCCGTGGTTTTTTCTATTGAGCATGTCGGCGTGGCTTGGCTTTTGTACCTGGGCCt                                                                                   >  2:712464/1‑150 (MQ=255)
                                                               tGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGCCGGGCATATGCTCAATGAGCCGTGGTTTTTTCTATTGAGCATGTCGGCGTGGCTTGGCTTTTGTACCTGGGCCtgt                                                                                 >  1:380993/1‑151 (MQ=255)
                                                                ggCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGCCGGGCATATGCTCAATGAGCCGTGGTTTTTTCTATTGAGCATGTCGGCGTGGCTTGGCTTTTGTACCTGGGCCtgtg                                                                                <  1:13444/151‑1 (MQ=255)
                                                                ggCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGCCGGGCATATGCTCAATGAGCCGTGGTTTTTTCTATTGAGCATGTCGGCGTGGCTTGGCTTTTGTACCTGGGCCtgt                                                                                 <  1:888659/150‑1 (MQ=255)
                                                                   ggTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGCCGGGCATATGCTCAATGAGCCGTGGTTTTTTCTATTGAGCATGTCGGCGTGGCTTGGCTTTTGTACCTGGGCCTGTgcgc                                                                             >  1:719482/1‑151 (MQ=255)
                                                                     tgtTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGCCGGGCATATGCTCAATGAGCCGTGGTTTTTTCTATTGAGCATGTCGGCGTGGCTTGGCTTTTGTACCTGGGCCTGTGCGCAc                                                                           >  2:502071/1‑151 (MQ=255)
                                                                      gtTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGCCGGGCATATGCTCAATGAGCCGTGGTTTTTTCTATTGAGCATGTCGGCGTGGCTTGGCTTTTGTACCTGGGCCTGTGCGCACt                                                                          <  1:960770/151‑1 (MQ=255)
                                                                       ttATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGCCGGGCATATGCTCAATGAGCCGTGGTTTTTTCTATTGAGCATGTCGGCGTGGCTTGGCTTTTGTACCTGGGCCTGTGCGCACtt                                                                         >  1:48121/1‑151 (MQ=255)
                                                                        tATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGCGTCATTGAGGCACTGCTTCTTGCCGGGCATATGCTCAATGAGCCGTGGTTTTTTCTATTGAGCATGTAGGCGTGCCTTGGCTTTTGTACCTGGGCCTGTGCGCAc                                                                           <  1:833953/148‑1 (MQ=255)
                                                                         aTCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGCCGGGCATATGCTCAATGAGCCGTGGTTTTTTCTATTGAGCATGTCGGCGTGGCTTGGCTTTTGTACCTGGGCCTGTGCGCACTTCa                                                                       <  2:381215/151‑1 (MQ=255)
                                                                          tCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGCCGGGCATATGCTCAATGAGCCGTGGTTTTTTCTATTGAGCATGTCGGCGTGGCTTGGCTTTTGTACCTGGGCCTGTGCGCACTTCa                                                                       <  1:910190/150‑1 (MQ=255)
                                                                           cAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGCCGGGCATATGCTCAATGAGCCGTGGTTTTTTCTATTGAGCATGTCGGCGTGGCTTGGCTTTTGTACCTGGGCCTGTGCGCACTTCACg                                                                     <  1:830958/151‑1 (MQ=255)
                                                                             gCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGCCGGGCATATGCTCAATGATCCGTGGTTTTTTCTATTGCGCATGTCGGCGTGGCTTGGCTTTTGTACCTGGGCCTGTGCACACTTCACGaa                                                                   <  1:279967/151‑1 (MQ=255)
                                                                              cAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGCCGGGCATATGCTCAATGAGCCGTGGTTTTTTCTATTGAGCATGTCGGCGTGGCTTGGCTTTTGTACCTGGGCCTGTGCGCACTTCACGAAt                                                                  <  1:438119/151‑1 (MQ=255)
                                                                              cAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGCCGGGCATATGCTCAATGAGCCGTGGTTTTTTCTATTGAGCATGTCGGCGTGGCTTGGCTTTTGTACCTGGGCCTGTGCGCACTTCACGAAt                                                                  >  2:528455/1‑151 (MQ=255)
                                                                               aaaaGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGCCGGGCATATGCTCAATGAGCCGTGGTTTTTTCTATTGAGCATGTCGGCGTGGCTTGGCTTTTGTACCTGGGCCTGTGCGCACTTCACGAAt                                                                  >  2:321872/1‑150 (MQ=255)
                                                                               aaaaGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGCCGGGCATATGCTCAATGAGCCGTGGTTTTTTCTATTGAGCATGTCGGCGTGGCTTGGCTTTTGTACCTGGGCCTGTGCGCACTTCACGAATa                                                                 <  1:417679/151‑1 (MQ=255)
                                                                                aaaGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGCCGGGCATATGCTCAATGAGCCGTGGTTTTTTCTATTGAGCATGTCGGCGTGGCTTGGCTTTTGTACCTGGGCCTGTGCGCACTTCACGAATaa                                                                <  2:216413/151‑1 (MQ=255)
                                                                                aaaGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGCCGGGCATATGCTCAATGAGCCGTGGTTTTTTCTATTGAGCATGTCGGCGTGGCTTGGCTTTTGTACCTGGGCCTGTGCGCACTTCACGAATa                                                                 >  1:734019/1‑150 (MQ=255)
                                                                                    ccTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGCCGGGCATATGCTCAATGAGCCGTGGTTTTTTCTATTGAGCATGTCGGCGTGGCTTGGCTTTTGTACCTGGGCCTGTGCGCACTTCACGAATAACg                                                              >  1:347601/1‑149 (MQ=255)
                                                                                     cTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGCCGGGCATATGCTCAATGAGCCGTGGTTTTTTCTATTGAGCATGTCGGCGTGGCTTGGCTTTTGTACCTGGGCCTGTGCGCACTTCACGAATAACGTc                                                            >  1:964441/1‑150 (MQ=255)
                                                                                       cGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGCCGGGCATATGCTCAATGAGCCGTGGTTTTTTCTATTGAGCATGTCGGCGTGGCTTGGCTTTTGTACCTGGGCCTGTGCGCACTTCACGAATAACGTcgc                                                          <  1:653829/150‑1 (MQ=255)
                                                                                        ggACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGCCGGGCATATGCTCAATGAGCCGTGGTTTTTTCTATTGAGCATGTCGGCGTGGCTTGGCTTTTGTACCTGGGCCTGTGCGCACTTCACGAATAACGTCGCGt                                                        >  2:639160/1‑151 (MQ=255)
                                                                                             cATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGCCGGGCATATGCTCAATGAGCCGTGGTTTTTTCTATTGAGCATGTCGGCGTGGCTTGGCTTTTGTACCTGGGCCTGTGCGCACTTCACGAATAACGTCGCGTATGc                                                    >  2:956639/1‑150 (MQ=255)
                                                                                                cGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGCCGGGCATATGCTCAATGAGCCGTGGTTTTTTCTATTGAGCATGTCGGCGTGGCTTGGCTTTTGTACCTGGGCCTGTGCGCACTTCACGAATAACGTCGCGTATGCAttt                                                >  2:935746/1‑151 (MQ=255)
                                                                                                  cTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGCCGGGCATATGCTCAATGAGCCGTGGTTTTTTCTATTGAGCATGTCGGCGTGGCTTGGCTTTTGTACCTGGGCCTGTGCGCACTTCACGAATAACGTCGCGTATGCATTTc                                               >  1:738940/1‑150 (MQ=255)
                                                                                                      cAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGCCGGGCATATGCTCAATGAGCCGTGGTTTTTTCTATTGAGCATGTCGGCGTGGCTTGGCTTTTGTACCTGGGCCTGTGCGCACTTCACGAATAACGTCGCGTATGCATTTCAACTg                                          <  2:327413/151‑1 (MQ=255)
                                                                                                      cAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGCCGGGCATATGCTCAATGAGCCGTGGTTTTTTCTATTGAGCATGTCGGCGTGGCTTGGCTTTTGTACCTGGGCCTGTGCGCACTTCACGAATAACGTCGCGTATGCATTTCAACTg                                          <  1:835356/151‑1 (MQ=255)
                                                                                                       aGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGCCGGGCATATGCTCAATGAGCCGTGGTTTTTTCTATTGAGCATGTCGGCGTGGCTTGGCTTTTGTACCTGGGCCTGTGCGCACTTCACGAATAACGTCGCGTATGCATTTCAACTgg                                         >  2:345071/1‑151 (MQ=255)
                                                                                                       aGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGCCGGGCATATGCTCAATGAGCCGTGGTTTTTTCTATTGAGCATGTCGGCGTGGCTTGGCTTTTGTACCTGGGCCTGTGCGCACTTCACGAATAACGTCGCGTATGCATTTCAACTgg                                         >  1:422811/1‑151 (MQ=255)
                                                                                                       aGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGCCGGGCATATGCTCAATGAGCCGTGGTTTTTTCTATTGAGCATGTCGGCGTGGCTTGGCTTTTGTACCTGGGCCTGTGCGCACTTCACGAATAACGTCGCGTATGCATTTCAACTgg                                         >  1:217852/1‑151 (MQ=255)
                                                                                                        gTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGCCGGGCATATGCTCAATGAGCCGTGGTTTTTTCTATTGAGCATGTCGGCGTGGCTTGGCTTTTGTACCTGGGCCTGTGCGCACTTCACGAATAACGTCGCGTATGCATTTCAACTgg                                         >  1:347186/1‑150 (MQ=255)
                                                                                                          ttGCTCGGAGCCATTGCGGCACTGCTTCTTGCCGGGCATATGCTCAATGAGCCGTGGTTTTTTCTATTGAGCATGTCGGCGTGGCTTGGCTTTTGTACCTGGGCCTGTGCGCACTTCACGAATAACGTCGCGTATGCATTTCAACTGGCgg                                      >  2:99442/1‑151 (MQ=255)
                                                                                                           tGCTCGGAGCCATTGCGGCACTGCTTCTTGCCGGGCATATGCTCAATGAGCCGTGGTTTTTTCTATTGAGCATGTCGGCGTGGCTTGGCTTTTGTACCTGGGCCTGTGCGCACTTCACGAATAACGTCGCGTATGCATTTCAACTGGCgg                                      <  2:346028/150‑1 (MQ=255)
                                                                                                            gCTCGGAGCCATTGCGGCACTGCTTCTTGCCGGGCATATGCTCAATGAGCCGTGGTTTTTTCTATTGAGCATGTCGGCGTGGCTTGGCTTTTGTACCTGGGCCTGTGCGCACTTCACGAATAACGTCGCGTATGCATTTCAACTGGCGGGc                                    <  2:719549/151‑1 (MQ=255)
                                                                                                              tCGGAGCCATTGCGGCACTGCTTCTTGCCGGGCATATGCTCAATGAGCCGTGGTTTTTTCTATTGAGCATGTCGGCGTGGCTTGGCTTTTGTACCTGGGCCTGTGCGCACTTCACGAATAACGTCGCGTATGCATTTCAACTGGCGGGCt                                   >  2:191934/1‑150 (MQ=255)
                                                                                                               cGGAGCCATTGCGGCACTGCTTCTTGCCGGGCATATGCTCAATGAGCCGTGGTTTTTTCTATTGAGCATGTCGGCGTGGCTTGGCTTTTGTACCTGGGCCTGTGCGCACTTCACGAATAACGTCGCGTATGCATTTCAACTGGCGGGCTac                                 <  1:422613/151‑1 (MQ=255)
                                                                                                                    ccATTGCGGCACTGCTTCTTGCCGGGCATATGCTCAATGAGCCGTGGTTTTTTCTATTGAGCATGTCGGCGTGGCTTGGCTTTTGTACCTGGGCCTGTGCGCACTTCACGAATAACGTCGCGTATGCATTTCAACTGGCGGGCTACACGGc                            <  1:579016/151‑1 (MQ=255)
                                                                                                                     cATTGCGGCACTGCTTCTTGCCGGGCATATGCTCAATGAGCCGTGGTTTTTTCTATTGAGCATGTCGGCGTGGCTTGGCTTTTGTACCTGGGACTGTGCGCACTTCACGAATAACGCCGCGTATGCATTTCAACTGGCGGGCTACACgg                             >  1:21184/1‑149 (MQ=255)
                                                                                                                          cGGCACTGCTTCTTGCCGGGCATATGCTCAATGAGCCGTGGTTTTTTCTATTGAGCATGTCGGCGTGGCTTGGCTTTTGTACCTGGGCCTGTGCGCACTTCACGAATAACGTCGCGTATGCATTTCAACTGGCGGGCTACACGGCTGCcat                      <  2:763958/151‑1 (MQ=255)
                                                                                                                          cGGCACTGCTTCTTGCCGGGCATATGCTCAATGAGCCGTGGTTTTTTCTATTGAGCATGTCGGCGTGGCTTGGCTTTTGTACCTGGGCCTGTGCGCACTTCACGAATAACGTCGCGTATGCATTTCAACTGGCGGGCTACACGGCTGCcat                      >  1:821425/1‑151 (MQ=255)
                                                                                                                                    tcttGCCGGGCATATGCTCAATGAGCCGTGGTTTTTTCTATTGAGCATGTCGGCGTGGCTTGGCTTTTGTACCTGGGCCTGTGCGCACTTCACGAATAACGTCGCGTATGCATTTCAACTGGCGGGCTACACGGCTGCCATCATCGCCttt            >  2:389475/1‑151 (MQ=255)
                                                                                                                                        gCCGGGCATATGCTCAATGAGCCGTGGTTTTTTCTATTGAGCATGTCGGCGTGGCTTGGCTTTTGTACCTGGGCCTGTGCGCACTTCACGAATAACGTCGCGTATGCATTTCAACTGGCGGGCTACACGGCTGCCATCATCGCCTTTCCg         <  1:299002/150‑1 (MQ=255)
                                                                                                                                        gCCGGGCATATGCTCAATGAGCCGTGGTTTTTTCTATTGAGCATGTCGGCGTGGCTTGGCTTTTGTACCTGGGCCTGTGCGCACTTCACGAATAACGTCGCGTATGCATTTCAACTGGCGGGCTACACGGCTGCCATCATCGCCTTTCCg         >  2:389179/1‑150 (MQ=255)
                                                                                                                                        gCCGGGCATATGCTCAATGAGCCGTGGTTTTTTCTATTGAGCATGTCGGCGTGGCTTGGCTTTTGTACCTGGGCCTGTGCGCACTTCACGAATAACGTCGCGTATGCATTTCAACTGGCGGGCTACACGGCTGCCATCATCGCCTTTCCGa        <  1:520040/151‑1 (MQ=255)
                                                                                                                                             gCATATGCTCAATGAGCCGTGGTTTTTTCTATTGAGCATGTCGGCGTGGCTTGGCTTTTGTACCTGGGCCTGTGCGCACTTCACGAATAACGTCGCGTATGCATTTCAACTGGCGGGCTACACGGCTGCCATCATCGCCTTTCCGATGGtt   <  2:852376/151‑1 (MQ=255)
                                                                                                                                             gCATAGGCTCAATGATCCGTGGTTTTTTCGATTGAGCATGTCGGCGTGGCTTGGGTTTTGTACCTGAGCCTGTGCGCACTTCACGCATACCGTCGCGTCTGCATTTCAACTGGCGGGCTCCACGGCTGCCATCATCGCCTTTCCGCTGGt    <  1:430182/150‑1 (MQ=255)
                                                                                                                                              cATATGCTCAATGAGCCGTGGTTTTTTCTATTGAGCATGTCGGCGTGGCTTGGCTTTTGTACCTGGGCCTGTGCGCACTTCACGAATAACGTCGCGTATGCATTTCAACTGGCGGGCTACACGGCTGCCATCATCGCCTTTCCGATGGTTa  <  2:971734/151‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
TTTAAATCTGGATGAACCCTATTGGGCGATGACCTCGGCTGCAGTGGTTAGCTTTCCCACCGTTGGCGGTGTTATCAGCAAAAGCCTCGGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGCCGGGCATACGCTCAATGAGCCGTGGTTTTTTCTATTGAGCATGTCGGCGTGGCTTGGCTTTTGTACCTGGGCCTGTGCGCACTTCACGAATAACGTCGCGTATGCATTTCAACTGGCGGGCTACACGGCTGCCATCATCGCCTTTCCGATGGTTA  >  NC_000913/1722240‑1722532

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: