Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,369,969	T→A	T128S (ACC→TCC)	nanK ←	N‑acetylmannosamine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,369,969	0	T	A	100.0%	209.1 / NA	56	T128S (ACC→TCC)	nanK	N‑acetylmannosamine kinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (23/33);  total (23/33)

CCACGCAACCTGTGCGTCCACAGCCGCAGACTGGGCCGTGTGGATCGGCAAGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGTGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAGTTGTTCCAGCGTTTTGACTAACGGAAAGTGTAGCAATCCACCAAGATT  >  NC_000913/3369828‑3370116                                                                                                                                              |                                                                                                                                                    ccACGCACCCTGTGGTTCCAAAGCCGCATACTGGGCCGTGTGCATCGGCAAGCGTATGCCCGAGATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAATTGGCAGCCGCACACTACACCGCCGCCAACGCCGGTGGAAACAGAGATAAAGa                                                                                                                                             <  1:900490/150‑1 (MQ=255)     agaaccggggggTCCGCAGCGGCAGGGTGGGCAGTGGGGAGGGACGAGGGGAGGCCCGAAATGCCCCGCGAGACCGCCAGGGCCGGTAAGCAGTGTGCAGCCGCTCAGTACAGCGCAACCAACGCCGGTGGAAACGGAGATaaa                                                                                                                                               <  1:438084/133‑1 (MQ=255)      cAACCTGTGCGTCCACAGCCGCAGACTGGGCCGTGTGGATCGGCAAGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCatat                                                                                                                                       >  2:886920/1‑151 (MQ=255)         ccTGTGCGTCCACAGCCGCAGACTGGGCCGTGTGGATCGGCAAGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCgg                                                                                                                                    >  1:749888/1‑151 (MQ=255)               cttCCACAGCCGCAAACTGGGCAGGGTGGATCGGCAAGCGTAGGCCCGATATCCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACTCCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATACCGGTtat                                                                                                                                <  1:868908/147‑1 (MQ=255)                gTCCACAGCCGCAGACTGGGCCGTGTGGAAAGGAGAGCGTATGCACGATATGCCCCGCCAGACCGCCATGGCCGGAAAGCAGTTTGCAGCCGCTAACTAAACCGCCGCCAACGCCGGTGGAAACGAAGATAAAGACCATCTCGGTTATATc                                                                                                                             >  1:440108/1‑151 (MQ=255)                  ccACAGCCGCAGACTGGGCCGTGTGGATCGGCAAGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCg                                                                                                                            <  1:302198/150‑1 (MQ=255)                    acaGCCGCAGACTGGGCCGTGTGGATCGGCAAGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCa                                                                                                                         <  2:469185/151‑1 (MQ=255)                         cGCAGACTGGGCCGTGTGGATCGGCAAGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATcc                                                                                                                      >  1:430539/1‑149 (MQ=255)                               cTGGGCCGGGTGGATCGGAAAGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGcc                                                                                                                <  1:737998/149‑1 (MQ=255)                                 gggCCGTGTGGATCGGCAAGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGaa                                                                                                            <  2:162037/151‑1 (MQ=255)                                    ccGTGTGGATCGGCAAGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACt                                                                                                         >  2:143539/1‑151 (MQ=255)                                     cGTGTGGATCGGCAAGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTc                                                                                                        >  1:167305/1‑151 (MQ=255)                                          ggATCGGCAAGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGccc                                                                                                   >  2:414690/1‑151 (MQ=255)                                               ggCAAGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCAtgct                                                                                              <  2:162183/151‑1 (MQ=255)                                                   aGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCgg                                                                                          >  1:616823/1‑151 (MQ=255)                                                        aTGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACCCCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTg                                                                                      <  1:362856/150‑1 (MQ=255)                                                           cccGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTgcg                                                                                    <  1:326582/149‑1 (MQ=255)                                                           cccGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGcgcg                                                                                  >  2:325993/1‑151 (MQ=255)                                                            ccGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGcgt                                                                                 >  2:401719/1‑151 (MQ=255)                                                                   gCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAAt                                                                          <  2:494996/151‑1 (MQ=255)                                                                     cccGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATgg                                                                        >  2:488006/1‑151 (MQ=255)                                                                        gCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCGCTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGAGCGTCGTTAATGGCa                                                                      <  1:305421/150‑1 (MQ=255)                                                                        gCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCCCTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCa                                                                      <  1:344870/150‑1 (MQ=255)                                                                             aCCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTc                                                                <  2:591649/151‑1 (MQ=255)                                                                                gCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGc                                                             >  1:685272/1‑151 (MQ=255)                                                                                 ccAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCa                                                            <  1:174799/151‑1 (MQ=255)                                                                                    gggCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAAt                                                         <  2:721348/151‑1 (MQ=255)                                                                                        cGGTAATCAGTTTGAAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTTTATCGCCATGCAGCACCTGACACTCAGCCCATGCTGCGCCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTgg                                                      >  1:426910/1‑150 (MQ=255)                                                                                        cGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGt                                                     <  2:895285/151‑1 (MQ=255)                                                                                           tAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTa                                                    >  2:415036/1‑149 (MQ=255)                                                                                           tAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTa                                                    >  1:205341/1‑149 (MQ=255)                                                                                              gCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAgttg                                               <  2:545881/151‑1 (MQ=255)                                                                                              gCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAgttg                                               <  1:724204/151‑1 (MQ=255)                                                                                                    tGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAGTTGTTcc                                           >  1:286144/1‑149 (MQ=255)                                                                                                     gCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAGTTGTTCCAg                                         <  1:143501/150‑1 (MQ=255)                                                                                                     gCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAGTTGTTCCAGc                                        <  2:366186/151‑1 (MQ=255)                                                                                                     gCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAGTTGTTCCAGc                                        >  1:73543/1‑151 (MQ=255)                                                                                                      cAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAGTTGTTCCAGCg                                       <  1:714917/151‑1 (MQ=255)                                                                                                         ccGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAGTTGTTCCAGCGttt                                    <  2:511455/151‑1 (MQ=255)                                                                                                          cGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAGTTGTTCCAGCGtttt                                   <  2:640626/151‑1 (MQ=255)                                                                                                           gCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAGTTGTTCCAGCGTTTTg                                  <  1:9386/151‑1 (MQ=255)                                                                                                             tcccTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAGTTGTTCCAGCGTTTTg                                  <  1:779851/146‑1 (MQ=255)                                                                                                                            cAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAGTTGTTCCAGCGTTTTGACTAACGGAAAGTGTa                  <  2:645115/150‑1 (MQ=255)                                                                                                                              aCGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCACATTGGTAAGTTGTTCCAGCGTTTTGACTAACGGAAAGTGTa                  >  1:697195/1‑148 (MQ=255)                                                                                                                              aCGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAGTTGTTCCAGCGTTTTGACTAACGGAAAGTGTAGc                >  2:695329/1‑150 (MQ=255)                                                                                                                               cGCCGGTGAAAACGGAGATAAAGCCCATACCCGTTATATCGCCATCCATCGCTTGAAACTCTGACCATCCTCCGCCCGGCGCGTCGTTACTGGCAATGGTCGGCAAATTGGTAAGTTGTTTCAGCGATTTGACTAACGGACAGCGTa                  >  1:779397/1‑147 (MQ=255)                                                                                                                                 ccGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAGTTGTTCCAGCGTTTTGACTAACGGAAAGTGTAGCAATc            >  1:110576/1‑151 (MQ=255)                                                                                                                                  cGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAGTTGTTCCAGCGTTTTGACTAACGGAAAGTGTAGCAATcc           <  1:712908/151‑1 (MQ=255)                                                                                                                                       gAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAGTTGTTCCAGCGTTTTGACTAACGGAAAGTGTAGCAATCCACCaa      <  1:841472/151‑1 (MQ=255)                                                                                                                                        aaaCGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAGTTGTTCCAGCGTTTTGACTAACGGAAAGTGTAGCAATccacca       >  2:970389/1‑149 (MQ=255)                                                                                                                                         aaCGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAGTTGTTCCAGCGTTTTGACTAACGGAAAGTGTAGCAATCCACCAAGa    <  2:430255/151‑1 (MQ=255)                                                                                                                                         aaCGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCACGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAGTTGTTCCAGCGTTTTGACTAACGGAAAGTGTAGCAATCCACCAAGa    <  2:68175/151‑1 (MQ=255)                                                                                                                                          aCGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAGTTGTTCCAGCGTTTTGACTAACGGAAAGTGTAGCAATccacca       >  1:904730/1‑147 (MQ=255)                                                                                                                                           cGGAGATATATACCATATCGGTTATATGTCCATACGGCGCCTGAAACTCCGCTCATGCTGCTGCCTGCGCTTCGTTAATGGCAATGGTCGGCAAATTGGTAAGTTGTTCGAGCGTTTTGACGAACGGAGAGTGTAGCAATCCACCAAGAtt  <  1:798286/151‑1 (MQ=255)                                                                                                                                           cGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAGTTGTTCCAGCGTTTTGACTAACGGAAAGTGTAGCAATCCACCAAGAtt  <  1:652205/151‑1 (MQ=255)                                                                                                                                              |                                                                                                                                                    CCACGCAACCTGTGCGTCCACAGCCGCAGACTGGGCCGTGTGGATCGGCAAGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGTGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAGTTGTTCCAGCGTTTTGACTAACGGAAAGTGTAGCAATCCACCAAGATT  >  NC_000913/3369828‑3370116

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑