Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,604,237	0	C	T	43.1%	57.2 / 28.6	58	S64L (TCG→TTG)	lsrD	autoinducer 2 import system permease protein
Reads supporting (aligned to +/- strand):  ref base C (8/25);  new base T (25/0);  total (33/25)
Fisher's exact test for biased strand distribution p-value = 1.52e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-01

CGTTATTGAGATTGTCGCATTTGGTGCAATTAACCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCG‑AT‑ATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGAT‑GCCGCTGGCGATACTCCTGACCTTACTGCTCGGCGCATTGTGCGGGCTGATCAACGCCGGATTAATTATCTATACCAAAGTTAA  >  NC_000913/1604091‑1604387                                                                                                                                                     |                                                                                                                                                        cGTTATTGAGATTGTCGCATTTGGTGCAATTAACCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCG‑AT‑ATTTCGt                                                                                                                                                       <  2:935954/149‑1 (MQ=255)  gTTATTGAGATTGTCGCATTTGGTGCAATTAACCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGGTCG‑AT‑ATTTTGttt                                                                                                                                                     >  1:1074364/1‑150 (MQ=255)   ttATTGAGATTGTCGCATTTGGTGCAATTAACCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGGTCG‑AT‑ATTTTGttt                                                                                                                                                     >  2:557464/1‑149 (MQ=255)     aTTGAGATTGTCGCATTTGGTGCAATTAACCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCG‑AT‑ATTttgttttgtt                                                                                                                                                 >  1:641495/1‑151 (MQ=255)     aTTGAGATTGTCGCATTTGGTGCAATTAACCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCG‑AT‑ATTTCGTTTGGtt                                                                                                                                                 <  2:623218/151‑1 (MQ=255)      ttGAGATTGTCGCATTTGGTGCAATTAACCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGGTCG‑AT‑ATTttgttttgtt                                                                                                                                                 >  2:647928/1‑150 (MQ=255)      ttGAGATTGTCGCATTTGGTGCAATTAACCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCG‑AT‑ATTttgttttgtt                                                                                                                                                 >  2:93342/1‑150 (MQ=255)        gagaTTGTCGCATTTGGTGCAATTAACCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCG‑AT‑ATTTCGTTTGGTTCg                                                                                                                                               <  2:368309/150‑1 (MQ=255)         agaTTGTCGCATTTGGTGCAATTAACCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCG‑AT‑ATTTCGTTTGGTTCGAc                                                                                                                                             <  2:326643/151‑1 (MQ=255)          gaTTGTCGCATTTGGTGCAATTAACCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGTTTG‑TT‑ATTTCTTTTGGtttggcc                                                                                                                                            >  1:94462/1‑146 (MQ=255)             tGTCGCATTTGGTGCAATTAACCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGGTTG‑GT‑CTTTCTTTTTGTTTGACCCTc                                                                                                                                         >  1:804759/1‑151 (MQ=255)              gTCGCATTTGGTGCAATTAACCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCG‑AT‑ATTTCGTTTGGTTCGGCCATCg                                                                                                                                        <  2:108975/151‑1 (MQ=255)                         tGCAATTAACCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATTG‑GT‑ATTTTTTTTGGGTCGGCCATCGGGCCCTgcg                                                                                                                               >  1:545233/1‑149 (MQ=255)                         tGCAATTAACCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCG‑AT‑ATTTTGTTTGGTTCGACCATCGGCCTCTGCGcc                                                                                                                             >  2:377915/1‑151 (MQ=255)                             aTTAACCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCG‑AT‑ATTTCGTTTGGTTCGACCATCGGCCTCTGCGCcattg                                                                                                                         <  2:100364/151‑1 (MQ=255)                               tAACCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCG‑AT‑ATTTCGTTTGGTTCGACCATCGGCCTCTGCGCcattgc                                                                                                                        <  1:23004/150‑1 (MQ=255)                                aaCCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCG‑GT‑ATTTTGTTTTGTTTGACCATCCGCCCCTGCGccccttca                                                                                                                       >  1:664683/1‑144 (MQ=255)                                aaCCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCG‑AT‑ATTTCGTTTGGTTCGACCATCGGCCTCTGCGCcattgcat                                                                                                                      <  2:287366/151‑1 (MQ=255)                                     gcgAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCG‑AT‑ATTTTGTTTTGTTCGACCATCGGCCTCTGCGCccttgcatttg                                                                                                                   >  2:758514/1‑147 (MQ=255)                                     gcgAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCG‑AT‑ATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGc                                                                                                                 <  2:899750/151‑1 (MQ=255)                                         aTGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGGTTG‑AT‑ATTTTTTTTTGTTTGACCATCCGCCTCTGCGCCCTTGCATTTGGCg                                                                                                                >  1:1062532/1‑148 (MQ=255)                                          tGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCG‑AT‑ATTTTGTTTTGTTTGGCCAACCGCCTCTTGGCCATTTCATTGGGCGTaat                                                                                                            >  2:858703/1‑149 (MQ=255)                                           gTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCG‑AT‑ATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACt                                                                                                            <  2:541645/150‑1 (MQ=255)                                             tAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCG‑AT‑ATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACTGt                                                                                                          <  2:763128/150‑1 (MQ=255)                                                   tcAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGGTCG‑GT‑ATTTTGTTTGGTTTGACCATCGGCCTCCGCGCCCCTGCATTTGGGGGGCTGTTTcca                                                                                                     >  1:960026/1‑147 (MQ=255)                                                   tcAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCG‑AT‑ATTTTGTTTTGTTTGACCATCGGCCTCTGCGCCCTTTCCTTGGGCGTACTGTTTTaaa                                                                                                    >  2:458323/1‑150 (MQ=255)                                                     aaTATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCG‑AT‑ATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACTGTTTCAAAgtg                                                                                                 <  2:904517/151‑1 (MQ=255)                                                       tatGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCG‑AT‑ATTTCGTTTTGTTTGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACTGTTTCAAAgtgg                                                                                                >  2:484192/1‑150 (MQ=255)                                                        atGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCG‑AT‑ATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACTGTTTCAAAgtggtg                                                                                              <  2:1067421/151‑1 (MQ=255)                                                          gTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCG‑AT‑ATTTTGTTCGGGTCGACCATCCGCCTCTGCGCCCTTTCATTGGGCGGACTGTTTTTAAATGGggtg                                                                                            >  2:653330/1‑151 (MQ=255)                                                          gTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCG‑AT‑ATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGtgtg                                                                                            <  2:788172/151‑1 (MQ=255)                                                          gTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCG‑AT‑ATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGtgtg                                                                                            <  2:32237/151‑1 (MQ=255)                                                          gTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGCGGCGGGATCG‑AT‑ATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCCTTGGGCGTACTGTTTTAAAGTGGtgtg                                                                                            >  1:169987/1‑151 (MQ=255)                                                           ttGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCG‑AT‑ATTTTGTTTTGGTTGACCATCGGCCTCTGCGCCCTTGCCTTTGGCGGACTGTTTTAAAATGGtgtg                                                                                            >  2:721963/1‑150 (MQ=255)                                                              cTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGGTCC‑TT‑ATTTTGCTTTGTTCGGCCCTCGGCCCCTGCCCCCTTTCATTGGGCGGACTCTTTTaaaaggg                                                                                                >  1:230986/1‑139 (MQ=255)                                                                  tCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCG‑GT‑ATTTTGTTTTGTTCGCCCATCCGCCTCTGCGCCATTGCATTGGGCGTACTGTTTTAAAGTGGGGTGGCgcggc                                                                                     >  1:531359/1‑146 (MQ=255)                                                                   cAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCG‑AT‑ATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGAT‑Gcc                                                                                   <  2:388795/150‑1 (MQ=255)                                                                       aCCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGGTTG‑TT‑ATTTTGTTTTGTTTGACCATCGGCCCCTGCGCCCCTGCATTGGGGGGACTGGTTCTAAGGGGGGTGcc                                                                                          >  1:582448/1‑140 (MQ=255)                                                                         cAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCG‑AT‑ATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGAT‑GCCGCTGGCg                                                                            <  2:395810/151‑1 (MQ=255)                                                                               cTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCG‑AT‑ATTTTGTTTTGTTCTGCCATCCGCCTCTGCGGCCTTGCATTGGGCGGTCTGTTTTTAAGGGGTGTGCCCGT‑GCCGCTGGGGATAc                                                                        >  1:86866/1‑149 (MQ=255)                                                                               cTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCG‑AT‑ATTTTGTTTTGTTCGACCATCGGCCCCTGCGCCATTGCATTGGGGGGACTGTTTTCAAGAGGTGTGCCGAT‑GCCGCTGGCGGTACTc                                                                      >  2:91192/1‑151 (MQ=255)                                                                               cTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCG‑AT‑ATTTTGTTTTGTTCGACCATCCGCCCCTGCGCCATTGCATTGGGGGTACTGTTTTAAAGTGGTGTGCCGAT‑GCCGCTGGCGGTACt                                                                       >  1:496091/1‑150 (MQ=255)                                                                                       gcattggcattgTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCG‑AT‑ATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGAT‑GCCGCTGGCGATACTCCTGa                                                                  <  1:721919/147‑1 (MQ=255)                                                                                       gcattggcattgTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCG‑AT‑ATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGAT‑GCCGCTGGCGATACTCCTGACCt                                                               <  2:38487/150‑1 (MQ=255)                                                                                        cattggcattgTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCG‑GT‑ATTTCGTTTTGTTCGACCATCGGCCTCTGCGCCCTTGCATTGGGCGGACTGTTTTTAAGTGGGGTGCCGGT‑GCCGCTGGGGGTACTCCTGACCtt                                                              >  1:371709/1‑150 (MQ=255)                                                                                        cattggcattgTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCG‑AT‑ATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGAT‑GCCGCTGGCGATACTCCTGACCTTa                                                             <  1:624662/151‑1 (MQ=255)                                                                                           tggcattgTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGGTCGTTT‑TTTTCTTTTGGTT‑GACCCACCGCCCCCTCGCCCTTTCCTTGGGGGGACTTTTTTTAAGGGGTGTGCCGAT‑GCCGcctgcggttatcc                                                                     >  1:814207/1‑128 (MQ=255)                                                                                            ggcattgTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGGACG‑AT‑ATTTTGTTTGGTTTGACCAACCGCCCCTTCGCCCTTTCCTTTTGCGGTCTGGTTCCAACGGGGGTGCCGCTGGCCGCTGG‑GATCCTCCTTGCCCTACtac                                                         >  2:117145/1‑149 (MQ=255)                                                                                            ggcattgTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCG‑AT‑ATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGAT‑GCCGCTGGCGATACTCCTGACCTTACTg                                                          <  2:265026/150‑1 (MQ=255)                                                                                               attgTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGGACG‑GTAATTTTTTTTGGTT‑GACCATCCGCCCCTGCGCCACTGCCTTTTGGGGACTGGTTCAAAACGGTGTGGCGAT‑GGCCCGGGGGAGACTCCCGTTCTTACTGc                                                         >  2:260322/1‑148 (MQ=255)                                                                                                  gTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGGTCG‑AT‑ATTTTCTTTTGTTCGCCCATCGGCCTCTGCGCCCCTTCCTTGGGCGGACTGTTTTTAAGTGGGGGGCCCGT‑GCCCCCCGCGGGACTCCCGGCCTTTCTGCTCgg                                                     >  1:604903/1‑149 (MQ=255)                                                                                                       ccTACCGCTAACGATGGTGATTGTCAGTGGCGGGGTCG‑AT‑ATTTCCTTTTGTTTTACCACCGGCCTCTGCGCCCTTGCCTTTGGCGGTCTGTTTTTAAGAGGGGTGCCGCT‑GCCCCCGGCGATGCTCCTGACCTTACTg                                                          >  1:566674/1‑139 (MQ=255)                                                                                                         tACCGCTAACGATGGTGATTGTCAGTGGCGGGATCG‑AT‑ATTTTGTTTTGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGGACTGTTTTAAAGTGGGGTGCCGAT‑GCCGCTGGCGATACTCCTGACCTTACTGCTCGGCGCATtgtg                                            >  2:476880/1‑151 (MQ=255)                                                                                                               tAACGATGGTGATTGTCAGTGGCGGGAGCG‑AT‑ATTTTGTTTTGTTCGACCATCGGCCCCTTCGCCCTTGCATTTGGGGTTCTGTTTTTAAGTGGGGTGGCCCT‑GCCGCTGGCGGTACCCCTGGCCCTTCTGCTCGGCGCATTGTGgggg                                        >  1:221506/1‑149 (MQ=255)                                                                                                                aaCGATGGTGATTGTCAGTGGCGGGGTCG‑AT‑ATTTTGTTTTGTTTGACCATCCGCCCCTGCGCCATTTCATTTGGCGTACTGTTTCAAAGTGGTGTGGCGAT‑GCCGCTGGCGGTACTCCTTACCTTAATGCTCGGCGCCTTTTGCggg                                        >  2:1008115/1‑148 (MQ=255)                                                                                                                       gTGATTGTCAGTGGCGGGATCG‑AT‑ATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGAT‑GCCGCTGGCGATACTCCTGACCTTACTGCTCGGCGCATTGTGCGGGCTGATCAACg                              <  2:641536/151‑1 (MQ=255)                                                                                                                       gTGATTGTCAGTGGCGGGATCG‑AT‑ATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGAT‑GCCGCTGGCGATACTCCTGACCTTACTGCTCGGCGCATTGTGCGGGCTGATCAACg                              <  1:911949/151‑1 (MQ=255)                                                                                                                        tGATTGTCAGTGGCGGGATCG‑GA‑ATTTTGTTTTGTTTGACCATCGGCCTCTGCGCCCTTGCATTGGGGGGACTGTTTTAAAGGGGGGTGGCGAT‑GCCGCCGGCGATACTCCTGACCTTTCTGCTCGGCGCCTTGTGGGGGGTGGTCAAccc                             >  1:982297/1‑149 (MQ=255)                                                                                                                                           cG‑AT‑ATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGAT‑GCCGCTGGCGATACTCCTGACCTTACTGCTCGGCGCATTGTGCGGGCTGATCAACGCCGGATTAATTATCTATAcc          <  2:34966/151‑1 (MQ=255)                                                                                                                                               t‑atTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGAT‑GCCGCTGGCGATACTCCTGACCTTACTGCTCGGCGCATTGTGCGGGCTGATCAACGCCGGATTAATTATCTATACCa         >  1:150043/1‑149 (MQ=255)                                                                                                                                                 atTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGAT‑GCCGCTGGCGATACTCCTGACCTTACTGCTCGGCGCATTGTGCGGGCTGATCAACGCCGGATTAATTATCTATACCaaa       <  1:607005/150‑1 (MQ=255)                                                                                                                                                     cGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGAT‑GCCGCTGGCGATACTCCTGACCTTACTGCTCGGCGCATTGTGCGGGCTGATCAACGCCGGATTAATTATCTATACCAAAGTTaa  <  2:400450/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                        CGTTATTGAGATTGTCGCATTTGGTGCAATTAACCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCG‑AT‑ATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGAT‑GCCGCTGGCGATACTCCTGACCTTACTGCTCGGCGCATTGTGCGGGCTGATCAACGCCGGATTAATTATCTATACCAAAGTTAA  >  NC_000913/1604091‑1604387

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑