Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,851,6540TG42.9% 109.9 / 19.8 70G218G (GGT→GGG) hypBGTP hydrolase involved in nickel liganding into hydrogenases
Reads supporting (aligned to +/- strand):  ref base T (13/27);  new base G (30/0);  total (43/27)
Fisher's exact test for biased strand distribution p-value = 9.89e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTT  >  NC_000913/2851509‑2851804
                                                                                                                                                 |                                                                                                                                                      
cacaGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTCCGTTACCGAAGGTGAAGa                                                                                                                                                   <  2:833759/151‑1 (MQ=255)
 acaGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTCCGTTACCGAAGGTGAAGAc                                                                                                                                                  <  2:425132/151‑1 (MQ=255)
 acaGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTCCGTTACCGAAGGTGAAGAc                                                                                                                                                  <  2:910436/151‑1 (MQ=255)
   aGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTCCGTTACCGAAGGTGAAGACa                                                                                                                                                 <  1:979890/150‑1 (MQ=255)
    gatgatTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGGTTTCCGTTTCCGAAGGTGAAGa                                                                                                                                                   >  1:1060677/1‑147 (MQ=255)
     atgatTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTCCGTTACCGAAGGTGAAGACAAAc                                                                                                                                              <  1:372068/151‑1 (MQ=255)
     atgatTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTCCGTTACCGAAGGTGAAGACAAAc                                                                                                                                              <  1:989561/151‑1 (MQ=255)
     atgatTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTCCGTTACCGAAGGTGAAGACAAAc                                                                                                                                              <  2:61622/151‑1 (MQ=255)
       gatTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCGGGCCATGTTTGATCTCGGTGAAAAACACAAAGTGGCGGTGGTTTCCCTTAACCAAGGGGacaaaaaa                                                                                                                                               >  2:602950/1‑141 (MQ=255)
       gatTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTCCCTTACCGAAGGGGGAAACAAAcc                                                                                                                                             >  2:705201/1‑150 (MQ=255)
             cGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTCCGTTACCGAAGGTGAAGACAAACCACTGaaa                                                                                                                                      <  1:772677/151‑1 (MQ=255)
             cGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTCCGTTACCGAAGGTGAAGACAAACCACTGaaa                                                                                                                                      <  1:546861/151‑1 (MQ=255)
                 gccgcACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTTCGTTACCGGAGGGGAAGAAAAACCACTTaaa                                                                                                                                      >  2:424827/1‑147 (MQ=255)
                    gcACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTTCGTTTCCGAAGGGGAAGACAAAACACTGaaa                                                                                                                                      >  1:527942/1‑144 (MQ=255)
                      aCCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGc                                                                                                                               >  2:189427/1‑149 (MQ=255)
                        cgcgTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCatat                                                                                                                           <  2:197118/151‑1 (MQ=255)
                         gcgTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCATATg                                                                                                                          <  2:300163/151‑1 (MQ=255)
                         gcgTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTCCGTTACCGAAGGGGAAGACAAAACACTGAAATATCCGCatat                                                                                                                           >  1:322529/1‑150 (MQ=255)
                              tGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTCCCTTTCCGAAGGGGGAGAAAAAACCATGAAATATCCGCCTATGttt                                                                                                                       >  2:665986/1‑149 (MQ=255)
                               gCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCATATGttt                                                                                                                       <  2:255065/148‑1 (MQ=255)
                                      gACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTCCGTTACCGAAGGGGAAGACAAAACACTGGAAAAACCGCATATGTTTgccgccgcc                                                                                                              >  1:178299/1‑150 (MQ=255)
                                       aCGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTc                                                                                                            <  1:709359/151‑1 (MQ=255)
                                       aCGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTCCGTTACCGAAGGGGGAGACAAACCACTGAAATATCCCCATATGTTTgccgccgcccc                                                                                                            >  2:703283/1‑149 (MQ=255)
                                               ggTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGGTTTTCGTTAACGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTtccgccgcccc                                                                                                            >  2:365208/1‑141 (MQ=255)
                                                   ttCTGTTTATCGAAAACGTTTGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTCCCTTTCCCAAGGTGAAGACAAAACCCTTAACTAACCGCATATGTTTTTCGCCGCCCCGCtttt                                                                                                      >  1:453875/1‑142 (MQ=255)
                                                   ttCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTCCGTTACCGAAGGGGAAGACAAAACACTGAAATATCCGCATATGTTTTCCGCCGCCTCGCTGAtgc                                                                                                    >  1:796410/1‑147 (MQ=255)
                                                     cTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTTCGTTACCGAAGGGGAAGACAAACCACTGAAATATCCGCATATGTTTTCCGCCGCCTCGCTGATGCTGCTc                                                                                               >  2:715648/1‑150 (MQ=255)
                                                        tttATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCCCCTCGCTGATGCTGCTcaa                                                                                             >  2:714905/1‑149 (MQ=255)
                                                          tATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGGTCTTTTCGTTTCCGAAGGGGAAGAAAAAACACTGAAATATCCCCATATGTTTTCCCCCCCCCCGCCTATGCTGCCcaacaa                                                                                          >  1:409848/1‑150 (MQ=255)
                                                           aTCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTCCGTTACCGGAGGGGAAGACAAAACACTGAAATATCCGCATATGTTTGGCGCCGCCTCGCTGGTGCTGCCCAACaaa                                                                                         >  1:977296/1‑150 (MQ=255)
                                                            tCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTCCGTTACCGAAGGGGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACaaa                                                                                         >  2:719145/1‑149 (MQ=255)
                                                             cGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTCCGTTTCCGAAGGGGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAAtt                                                                                      >  2:82585/1‑151 (MQ=255)
                                                              gAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTTCGCTACCGAAGGGGAAGAAAAAACCCTCAAAAATCCCCATATGTTTTTCGCCGCCTCCCTGATGCTGCTCCACAAAAtt                                                                                      >  2:867666/1‑150 (MQ=255)
                                                                       ggCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGGTCTTTTCGTTACCGAAGGGGGAGACAAAACACTGAAATATCCGCCTATGTTTTCCCCCGCCTCGCTGATGCTGCTCAACAAAATTTAcccggt                                                                              >  2:627319/1‑145 (MQ=255)
                                                                          aaCCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGGGCTTTTCGTTACCCAAGGGGGAGAAAAAACACCGGAAAAACCGCACATGTTTTCCGCCCCCTCGCCGATGGTGCTCAACAAAATTTAACTGTTgtc                                                                           >  1:322032/1‑147 (MQ=255)
                                                                           aCCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTTCGTTACCGAAGGGGAAGACAAAACACCGAAAAATTCGCACATGTTTGCCGCCGCCCCGCCGATGCTTCTCAACCAAATTTACCCGTTTCCGt                                                                         >  1:486779/1‑150 (MQ=255)
                                                                           aCCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTa                                                                        <  2:1049555/151‑1 (MQ=255)
                                                                            ccTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTTCGTTACCGAAGGGGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTAt                                                                       >  2:193436/1‑151 (MQ=255)
                                                                             cttgTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGCACTTTTCGTTACCGAAAGGGTAGACAAACCACTTAAATATTCCCACATGTTTGCCCCCGCCTCGCTGATGCTTCCCAACAAAGGTGATCTGtttgcgt                                                                         >  2:243761/4‑143 (MQ=255)
                                                                             cTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTTCGTTACCGAAGGGGAAGACAAACCAATGAAATATCCGCATATGTTTTCCCCCGCCTCGCCGATTCTGCTCAACAAAATTGACCTGTTGCCGTa                                                                        >  2:243744/1‑149 (MQ=255)
                                                                                 tATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTCCGTTACCGCAGGGGAAGAAAAAACACTGGAATATCCGCATATTTTTGCCGCCGCCCCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTAtc                                                                      >  1:714390/1‑147 (MQ=255)
                                                                                   tGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGGTTTCCGTTACCGAAGGGGAAGACAAAACACCGAAATATTCGCATATTGTTGCCGCCCCCTCTCCGGTGCTTCTCAACAAAGATGACCTGTTTcc                                                                           >  2:1009112/1‑140 (MQ=255)
                                                                                     cccGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTCCGTTACCGGAGGGGAAGACAAACCACTGAAATATCCGCCTATGTTTGCCGCCGCCTCGCTGATGGTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACttt                                                              >  1:598864/1‑151 (MQ=255)
                                                                                      ccGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTCCGTTTCCGAAGGTGAAGACAAAACACTGAAATATCCCCCTATGTTTGCCGCCGCCCCGCTGATGCTGCTCCACAAAATTTACCTTTTTCCGGATCTCCACttt                                                              >  1:426614/1‑150 (MQ=255)
                                                                                       cGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTCCGTTACCGAAGGGGAAGACAAAACACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACttt                                                              >  2:112579/1‑149 (MQ=255)
                                                                                              cTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTTCGTTACCGAAGGGGAAGACAAACCACTGAAATATTCGCATATTTTTTCCGCCGCCTCGCCGATGCTGCCCAACAAAATTGACCCGTTTTCGTATCTCAACTTTTACggt                                                        >  1:1036536/1‑146 (MQ=255)
                                                                                                tCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTCCGTTAACGCAGGGGAAGACAAAACAATGAAATATCCGCATATGTTTGGCGCCGCCTCGCTGATGCTGCTCAAAAAAGTTGACCCTGTGCCGTATTTCAACTTTGaaggttga                                                     >  2:115380/1‑142 (MQ=255)
                                                                                                 cGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTGACGTTGaaaa                                                   <  1:763305/150‑1 (MQ=255)
                                                                                                    tctcGGTGAAAAACACAAAGTGGCGGTGCTTTCCGTTACCGAAGGGGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCCCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTGTCGTTTAAAAggg                                                >  1:1055392/1‑148 (MQ=255)
                                                                                                     ctcGGTGAAAAACACAAAGTGGCGGTGCTTTCCGTTACCGAAGGGGAAGACAAACCACTGAAATATCCGCATATGTTTTCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCCGTTGCCGTATCTCAACTTTGACGTTGAAAAGTg                                                >  2:262372/1‑149 (MQ=255)
                                                                                                       cGGTGAAAAACACAAAGTGGCGGTGCTTTCCGTTACCTAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTGACGTTGAAAAg                                                  <  2:992183/145‑1 (MQ=255)
                                                                                                       cGGTGAAAAACACAAAGTGGCGGTGCTTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTGACGTTGAAAAGTGc                                               <  2:247205/148‑1 (MQ=255)
                                                                                                       cGGTGAAAAACACAAAGTGGCGGTGCTTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTGACGTTGAAAAGTGCAt                                             <  2:962156/150‑1 (MQ=255)
                                                                                                         gTGAAAAACACAAAGTGGCGGTGCTTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTGACGTTGAAAAGTGCATCGc                                          <  1:56932/151‑1 (MQ=255)
                                                                                                            aaaaaCACAAAGTGGCGGTGCTTTCCGTTTCCGGAGGGGAAGACAAACCACAGAAAAATCCGCATTTGTTTTGCGCCGCCCCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAAATTTGACGTTGAAAAGTGCATCGcc                                         >  1:7745/1‑149 (MQ=255)
                                                                                                            aaaaaCACAAAGTGGCGGTGCTTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTGACGTTGAAAAGTGCATCGCCTg                                       <  2:420854/151‑1 (MQ=255)
                                                                                                            aaaaaCACAAAGTGGCGGTGCTTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTGACGTTGAAAAGTGCATCGCCTg                                       <  2:17224/151‑1 (MQ=255)
                                                                                                             aaaaCACAAAGTGGCGGTGCTTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTGACGTTGAAAAGTGCATCGCCTgc                                      <  2:1066581/151‑1 (MQ=255)
                                                                                                                       gTGGCGGTGCTTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAg                            <  1:24874/151‑1 (MQ=255)
                                                                                                                       gTGGCGGTGCTTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAg                            <  1:417236/151‑1 (MQ=255)
                                                                                                                               gCTTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCa                    <  2:53634/151‑1 (MQ=255)
                                                                                                                                 tttCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATcc                     >  2:115880/1‑148 (MQ=255)
                                                                                                                                 tttCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATcc                     >  2:891156/1‑148 (MQ=255)
                                                                                                                                     cGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAAtt              >  2:84757/1‑151 (MQ=255)
                                                                                                                                          ccGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAat         >  1:865701/1‑151 (MQ=255)
                                                                                                                                          ccGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAat         <  2:487578/151‑1 (MQ=255)
                                                                                                                                           cGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAatc        >  2:254048/1‑151 (MQ=255)
                                                                                                                                           cGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAatc        <  1:283876/151‑1 (MQ=255)
                                                                                                                                             aaGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAatcat      >  2:315442/1‑151 (MQ=255)
                                                                                                                                              aGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAatcatc     >  2:965521/1‑151 (MQ=255)
                                                                                                                                                 tGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCtt  >  1:677129/1‑151 (MQ=255)
                                                                                                                                                 |                                                                                                                                                      
CACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTT  >  NC_000913/2851509‑2851804

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑