Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,705,661 | 0 | T | C | 43.3% | 86.7 / 12.5 | 60 | I44V (ATC→GTC) | dppB | dipeptide/heme ABC transporter permease |
Reads supporting (aligned to +/- strand): ref base T (28/6); new base C (1/25); total (29/31) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 3.19e-10 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GCACGAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTTCAGCCAGCAGCTGCGCGTGACGCTCTGGGGAGATCCCACGTTCGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCAAATGTGAGAAGGGTAATACCGATAAACGTGGGGATGACGAGTCCCAAACGTCGGAGAATAAACTGCAACATAACCCGGATTCTCTAT > NC_000913/3705511‑3705806 | gCACGAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTTCAGCCAGCAGCTGCGCGTGACGCTCTGGGGAGAt < 2:826348/151‑1 (MQ=255) cACGAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTTCAGCCAGCAGCTGCGCGTGACGCTCTGGGGAGATc < 2:181084/151‑1 (MQ=255) ttCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTTCAGCCAGCAGCTGCGCGTGACGCTCTGGGGAGATCCCACGTTcg > 2:343561/1‑151 (MQ=255) ttCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTTCAGCCAGCAGCTGCGCGTGACGCTCTGGGGAGATCCCACGTTcg > 2:235047/1‑151 (MQ=255) tCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTTCAGCCAGCAGCTGCGCGTGACGCTCTGGGGAGATCCCACGTTcg < 1:448424/150‑1 (MQ=255) cccAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTTCAGCCAGCAGCTGCGCGTGACGCTCTGGGGAGATCCCACGTTcgcc > 2:597382/1‑151 (MQ=255) tGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTTCAGCCAGCAGCTGCGCGTGACGCTCTGGGGAGATCCCACGTTCGCCCGCCATGatcat > 1:829766/1‑151 (MQ=255) cGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTTCAGCCAGCAGCTGCGCGTGACGCTCTGGGGAGATCCCACGTTCGCCCGCCATGatcatc > 1:116373/1‑150 (MQ=255) tttCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTTCAGCCAGCAGCTGCGCGTGACGCTCTGGGGAGATCCCACGTTCGCCCGCCATGATCATCACCgg > 1:357882/1‑150 (MQ=255) tCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTTCAGCCAGCAGCTGCGCGTGACGCTCTGGGGAGATCCCACGTTCGCCCGCCATGATCATCACCGGATc > 2:952154/1‑151 (MQ=255) aaTTGAAATGCCTAGACCGCCATGCAAACCGCCCAAAATGAAAGGGAGATCCTGCTCCCACATCGGTTTATCTAAGCCGAGTCCAGCCAGCAGCGGCGCGGGCCGCTCGGGGGAGACCCCACGTCCGCCCGCCATGATCATCACCGGATCg < 1:101740/150‑1 (MQ=255) ttaaaaTCCCTAGACCCCCATGAATACCCCCCAAAATGTAATGGAGATCCTGCGCCCACACCGTTTTATCAAAGCCGATTTCACCCAGCAGCGGCGCGTGCCGCTGGGGGGAGACCCCCCGTTCCCCCGCCATGATCATCACCGGATCGc < 2:757509/147‑1 (MQ=255) aaaaTCCCTGGACCGCAATGCAAACCCCCCAAAATTTAAGGAAGATACTGCCGCCACATCGGTTTATCTAACCCGATTCCAGCCAGCAGCGGCGCGTGCCGCGCTGGGAAGATCCCACGTGCGCCCGCCATGATCATCACCGGATCGCCCg < 2:326057/150‑1 (MQ=255) aaaTTCCTAGACCCCCTTGCTTAACGCCTAAATTGTAAGGGAAATCCTCCTGCCACATCGGTTTATATAAGCCGATTCCAGCCAGCCGCTGCGCGTGACGTTGGGGGGAGACCCCACGTTCGCCCGCCATGATCATCACCGGATCGCCCgg < 2:279456/151‑1 (MQ=255) aaTGCCTAGATCGCCATGCATAACCCCCAAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTTCAGCCAGCAGCTGCGCGTGCCGCTCGGGGGAGATCCCACGTTCGCCCGCCATGATCATCACCGGATCGCCCggg < 2:140844/151‑1 (MQ=255) aaTCCCTAGCCCCCCATGCATACCCCCCAAATTGTAAGGGAGATCCTGCTCCCACATCTTTTTATCTAACCCGTTTTCACCCAGCGGCTGCGCTTGCCGCTGTGGGGAGCCCCCCCTTTCGCCCGCCATGATCATCACCGGATCGCCCggg < 1:1011772/151‑1 (MQ=255) aTGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTTCAGCCAGCAGCTGCGCGTGACGCTCTGGGGAGATCCCACGTTCGCCCGCCATGATCATCACCGGATCGCCCggg > 2:925356/1‑150 (MQ=255) gCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTTCAGCCAGCAGCTGCGCGTGACGCTCTGGGGAGATCCCACGTTCGCCCGCCATGATCATCACCGGATCGCCCGGGATc > 2:612604/1‑151 (MQ=255) tAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTTCAGCCAGCAGCTGCGCGTGACGCTCTGGGGAGATCCCACGTTCGCCCGCCATGATCATCACCGGATCGCCCGGGATCAtg > 2:413730/1‑151 (MQ=255) cATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTTCAGCCAGCAGCTGCGCGTGACGCTCTGGGGAGACCCCACGTTCGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACaa > 2:972467/1‑150 (MQ=255) aTAACCCCCAAAATGTAATGGAGATCCTGCTGCCACACCGTTTTATCAAAGCCGAGTTCAGCCAGCAGCGGCGCGTGCCGCTCGGGGGAGACCCCACTTCCGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCa < 2:330186/150‑1 (MQ=255) tAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTTCAGCCAGCAGCTGCGCGTGACGCTCTGGGGAGATCCCACGTTCGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCaa > 2:482462/1‑150 (MQ=255) accGCCCAAAATATAAGGGGGATTCTGCCGCCACACCGTTTTATCTACCCGGATTAAGGCCAGCAGCTGCGCGGGCCGCTCGGGGGAGCCCCCCGTTTCGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCa < 1:310754/146‑1 (MQ=255) aaCGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTTCAGCCAGCAGCTGCGCGTGACGCTCTGGGGAGATCCCACGTTCGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCAAAt > 2:456870/1‑151 (MQ=255) aaCGCCCAAAATGTAAGGGAGTTACTGCTCCCACATCGTTTTATCAAGGCCGAGTTCACCCGGCAGCTGCGCGGGCCGCTGTGGGGATACCCCCCGTTCGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCa < 2:878630/148‑1 (MQ=255) cccccaAAATGTAAGGGAGATACTGCTGCCACATCGTTTTATCTAAGCCGAGTCCAGCCAGCAGCTGCGCGTGACGCTCTGGGGAGACCCCACGTTCGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCAAAtg < 2:220413/148‑1 (MQ=255) tGTAATGGAGATACTGCCCCCCCATCGGTTTATCAAGGCCGTGTTCCGCCGGGCGCGGCCCGGGCCGCTGGGGGGAGACCCCGCGTCCGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCAAATGTGAGAAggg < 1:885803/150‑1 (MQ=255) aagggAGATCCTCCTCCCCCATCGTTTTACCTAAGCCGAGTTCAGCCAGCAGCTGGGCGTGCCGCTGGGGGGAGACCCCCCGTTCCCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCAAATGTGAGAAGGGTa < 2:29415/146‑1 (MQ=255) ggAGATCCTGCTCCCACATGGTTTTATCTAAGCCGATTCCAGCCAGCGGCGGCGCGGGCCGCTGGGGGGAGACCCCGCGTCCGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCAAATGTGAGAAGGGTAATAcc < 1:413709/151‑1 (MQ=255) agaTACTGCTGCCACATCGGTTTATCTAAGCCGAGTTCAGCCAGCAGCTGCGCGTGACGCTCTGGGGAGATCCCACGTTCGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCAAATGTGAGAAGGGTAATACCg > 1:176451/1‑150 (MQ=255) aTACTGCCGCCACATCGGTTTATCTAAGCCGAGTTCAGCCAGCAGCTGCCCGTGACGCTCTGGCGAGATCCCACGTTCGCCCGCCATGATCATCACCGCCTCGCCCGGGCTCATGTCGACAAACGCAATTGCGAGAAGGGTAATACCGAt > 1:597868/1‑150 (MQ=255) ctgctcCCACATGGGTTTATAAAAGCCGATTACGGCCAGCAGCGGCGGGGGCCGCTGGGGGGAGACCCCGCGTCCGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCAAATGTGAGAAGGGTAATACCGATaa < 1:72837/149‑1 (MQ=255) gctccCACATCGTTTTATCAAAGCCGAGTCCAGCCAGCAGCGGCGCGTGACGTTGGGGGGAGACCCCCCGTTCCCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCAAATGTGAGAAGGGTAATACCGATaa < 2:253023/147‑1 (MQ=255) ctgcCACATCGGTTTATCTAAGCCGAGTTCAGCCAGCAGCTGCGCGTGACGCTCTGGGGAGATCCCACGTTCGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCAAATGTGAGAAGGGTAATACCGATAAACGTg > 2:350261/1‑151 (MQ=255) ccACATCGTTTTATCAAAGCCGATTTCAGCCAGCAGCGGCGCGTGACGCTCGGGGGAGACCCCACGTCCGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCAAATGTGAGAAGGGTAATACCGATAAACGTgggg < 2:105383/151‑1 (MQ=255) acaTCGGTTTATCTAAGCCGGTTCCACCCAGCAGCCGGGCGGGCGGCTGGGGGGGGACCCCACGTCCGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCAAATGTGAGAAGGGTAATACCGATAAACg < 1:840473/144‑1 (MQ=255) acaTCGGTTTATCTAAGCCGAGTTCAGCCAGCAGCTGCGCGTGACGCTCTGGGGAGATCCCACGTTCGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCAAATGTGAGAAGGGTAATACCGATAAACGTGGGGAt > 2:673549/1‑151 (MQ=255) gttttATCAAGGCCGAGTCCAGCCAGCAGCTGCGCGGGCCGCTGGGGGGAGACCCCCCGTCCGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCAAATGTGAGAAGGGTAATACCGATAAACGTGGGGATGACg < 1:977766/148‑1 (MQ=255) aTCTAAGCCGAGTTCAGCCAGCAGCTGCGCGTGACGCTCTGGGGAGATCCCACGTTCGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCAAATGTGAGAAGGGTAATACCGATAAACGTGGGGATGACGAGTccc > 2:919265/1‑151 (MQ=255) aaGCCGAGTTCAGCCAGCAGCTGCGCGTGACGCTCTGGGGAGATCCCACGTTCGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCAAATGTGAGAAGGGTAATACCGATAAACGTGGGGATGACGAGTCCCAAAc > 1:282811/1‑151 (MQ=255) aGCCGAGTTCAGCCAGCAGCTGCGCGTGACGCTCTGGGGAGATCCCACGTTCGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCAAATGTGAGAAGGGTAATACCGATAAACGTGGGGATGACGAGTCCCAAACg > 1:709177/1‑151 (MQ=255) aGTTCAGCCAGCAGCTGCGCGTGACGCTCTGGGGAGATCCCACGTTCGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCAAATGTGAGAAGGGTAATACCGATAAACGTGGGGATGACGAGTCCCAAACGTCgg > 1:941699/1‑150 (MQ=255) tCAGCCAGCAGCTGCGCGTGACGCTCTGGGGAGATCCCACGTTCGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCAAATGTGAGAAGGGTAATACCGATAAACGTGGGGATGACGAGTCCCAAACGTCGgaga > 2:541242/1‑150 (MQ=255) ccacccagcGGCGGCGCGTGCCGCGGGGGGGAGACCCCCCGTCCGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCAAATGTGAGAAGGGTAATACCGATAAACGTGGGGATGACGAGTCCCAAACGTCGgaga < 1:685542/146‑1 (MQ=255) agccagcAGCTGCGCGTGACGCTCTGGGGAGATCCCACGTTCGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGCGGACAAAGGCAAATGTGAGAAGGGTAATACCGATAAACGTGGGGATGACGAGTCCCAAACGTCGGAGAAt > 2:305008/1‑150 (MQ=255) gcggcgcGTGGCGCTGTGGGGAGATCCCAGGGCCGACCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCAAATGTGAGAAGGGTAATACCGATAAACGTGGGGATGACGAGTCCCAAACGTCGGAGAATAAACTGCa < 1:776067/147‑1 (MQ=255) gcgcGGGACGCTCGGGGGAGACCCCACGTTCGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCAAATGTGAGAAGGGTAATACCGATAAACGTGGGGATGACGAGTCCCAAACGTCGGAGAATAAACTGCaa < 1:22800/148‑1 (MQ=255) cgcgTGACGCTCTGGGGAGATCCCACGTTCGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCAAATGTGAGAAGGGTAATACCGATAAACGTGGGGATGACGAGTCCCAAACGTCGGAGAATAAACTGCAACAt > 1:29699/1‑150 (MQ=255) gtgggccgcgggggggAGACCCCGCGTCCGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCAAATGTGAGAAGGGTAATACCGATAAACGTGGGGATGACGAGTCCCAAACGTCGGAGAATAAACTGCAACa < 1:625747/136‑1 (MQ=255) gccgccggggggAGTTCCCCCGTTCCCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCAAATGTGAGAAGGGTAATACCGATAAACGTGGGGATGACGAGTCCCAAACGTCGGAGAATAAACTGCAACATAAccc < 2:572246/142‑1 (MQ=255) aCGCTCTGGGGAGATCCCACGTTCGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCAAATGTGAGAAGGGTAATACCGATAAACGTGGGGATGACGAGTCCCAAACGTCGGAGAATAAACTGCAACATAACCCgg > 2:302231/1‑151 (MQ=255) gcgcgggggAGCCCCCACGTCCGCCCGCCATGATCATCACCGAATCGCCCGGGATCATGTGGACAAAGGCAAATGTGAGAAGGGTAATACCGATAAACGTGGGGATGACGAGTCCCAAACGTCGGAGAATAAACTGCAACATAAccc < 1:886993/142‑1 (MQ=255) ctctGGGGAGATCCCACGTTCGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCAAATGTGAGAAGGGTAATACCGATAAACGTGGGGATGACGAGTCCCAAACGTCGGAGAATAAACTGCAACATAACCCGGAtt > 1:243051/1‑151 (MQ=255) ggggggAGACCCATCGTGCAACCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCAAATGTGAGAAGGGTAATACCGATAAACGTGGGGATGACGAGTCCCAAACGTCGGAGAATAAACTGCAACATAACCCGGATtct < 1:631792/149‑1 (MQ=255) gggggAGACCCCTCGTTCGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCAAATGTGAGAAGGGTAATACCGATAAACGTGGGGATGACGAGTCCCAAACGTCGGAGAATAAACTGCAACATAACCCGGa < 2:68843/145‑1 (MQ=255) gggggAGACCCCCCGTCCGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCAAATGTGAGAAGGGTAATACCGATAAACGTGGGGATGACGAGTCCCAAACGTCGGAGAATAAACTGCAACATAACCCGGATtctc < 1:371945/150‑1 (MQ=255) ggggAGATCCCACGTTCGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCAAATGTGAGAAGGGTAATACCGATAAACGTGGGGATGACGAGTCCCAAACGTCGGAGAATAAACTGCAACATAACCCGGATTctct > 2:242889/1‑151 (MQ=255) ggggAGACCCCCCTTTCGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCAAATGTGAGAAGGGTAATACCGATAAACGTGGGGATGACGAGTCCCAAACGTCGGAGAATAAACTGCAACATAACCCGGa < 1:931117/145‑1 (MQ=255) gggAGCCCCCCCGTCCGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCAAATGTGAGAAGGGTAATACCGATAAACGTGGGGATGACGAGTCCCAAACGTCGGAGAATAAACTGCAACATAACCCGGATTCTCta < 1:53580/151‑1 (MQ=255) agaTCCCACGTTCGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCAAATGTGAGAAGGGTAATACCGATAAACGTGGGGATGACGAGTCCCAAACGTCGGAGAATAAACTGCAACATAACCCGGATTCTCtat > 2:500700/1‑149 (MQ=255) agaTCCCACGTTCGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCAAATGTGAGAAGGGTAATACCGATAAACGTGGGGATGACGAGTCCCAAACGTCGGAGAATAAACTGCAACATAACCCGGATTCTCtat > 2:468222/1‑149 (MQ=255) | GCACGAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTTCAGCCAGCAGCTGCGCGTGACGCTCTGGGGAGATCCCACGTTCGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCAAATGTGAGAAGGGTAATACCGATAAACGTGGGGATGACGAGTCCCAAACGTCGGAGAATAAACTGCAACATAACCCGGATTCTCTAT > NC_000913/3705511‑3705806 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |