| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,042,993 | 0 | C | T | 43.8% | 22.2 / 10.6 | 32 | F193F (TTC→TTT) | srkA | Cpx stress response Thr/Ser protein kinase; MazF antagonist protein |
| Reads supporting (aligned to +/- strand): ref base C (11/7); new base T (14/0); total (25/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.05e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGT‑CCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATG > NC_000913/4042851‑4043143 | ccATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCt < 2:176657/151‑1 (MQ=255) ggTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCCGTCTGCgg > 2:888228/1‑150 (MQ=255) tttGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGGAGATTTTCCCCTTCTggg > 1:857254/1‑145 (MQ=255) ttGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTa < 1:779075/150‑1 (MQ=255) aaCGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTAcc > 2:642857/1‑135 (MQ=255) aaCGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCg < 1:642816/143‑1 (MQ=255) atatCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTCCCGTTTTGCgggcccatgg > 1:299271/1‑139 (MQ=255) atatCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCCTTCTGCGGGCACATGgaga > 1:818598/1‑150 (MQ=255) ctcATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGCTCTGCGGCTCCATGGAGACTg > 1:411153/1‑149 (MQ=255) ttGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTTCGGCCACATGGGGACTGCCAc > 1:1083999/1‑149 (MQ=255) tGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGGTTTTCGGGCACATTGAGACCGccccccc > 1:547647/1‑146 (MQ=255) gAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTGCGGCTACATGGAGACTGCCACGcc > 2:579469/1‑150 (MQ=255) tGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGGAGAATTTACTGCTCTGCGGCGACCAGGAGACTGCCGCGCCGGGAATAttgt > 1:767040/1‑146 (MQ=255) aGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTGCGGCTACCTGGGGAATGGCCCGCCGGGGATATTttcttg > 1:731472/1‑144 (MQ=255) ggACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCCCCCTTCTGCGGCCACCTGGAGACCGCCCCCCCCGGGATATTCTCTGgcgc > 1:1018841/1‑148 (MQ=255) cacTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGGATTCACCGTTCTGGGGCTACATGGGGACTGCCACGCCGGGGAAATTCTCTTGCGGGGTGGG‑ccca > 2:720053/1‑148 (MQ=255) cTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGGTCTTCGGCTCCATGGAGAATGCCACCCCCGGGATATTCTCTGGCGCGATGGT‑CCCAtgt > 2:577714/1‑151 (MQ=255) tACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTTTGCGGGTACATGGGGACTGCCACCCCGGGGATATTTTCTTGCGCCATGGT‑CCAAtgttttt > 1:571071/1‑149 (MQ=255) gCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGT‑CCAATGTTTGTTGATCTGGATGATGCACGTa < 1:382667/151‑1 (MQ=255) gCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCCTTCTGCGGCTACATGGAGAATGCCACCCCGGGGAAATTCTTTTGCGCGATGGG‑CCAATGTTTGTTGGTCTGGGTGGTGCCCGt > 1:202412/1‑150 (MQ=255) cATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCCCCGTTCTGCGGCTTCCTGGAGAATGCCCCCCCGGGGATATTTTCTGGCGCGGTGGG‑CCAATGTTTTTTGGTCTGGATGATGCACGt > 1:630395/1‑149 (MQ=255) aaaGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGT‑CCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAg < 2:144963/151‑1 (MQ=255) gACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTTCGGCCACATGGAGGCCGCCCCGCCGGGGATATTTTCTGGCGCGGTGGT‑CCCATGGTTGTTGGTCTGGGTGATGCACGTAATGGTCCAGCCGt > 1:243819/1‑150 (MQ=255) gATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGGTCTGCGGCTACATGGGGACTGCCACGCCGGGGATATTCTCTGGCGCGATGGG‑TCCATGTTTGTTTGTCTGGATGATGCACGTAATGGTCCAGCCGGTCAgg > 2:943362/1‑151 (MQ=255) gAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTAACGCTCTTTGGCTCCATGGAGAAAGGCAACCCGGGGATAATGTTTCGCGTGGAGGTCCCAATGTTT‑TTGCTCTGGGTGATGCAGGTAATGGGCCAGCCGTTCAGGGtt > 1:691891/1‑151 (MQ=255) cgccgTTACAGCACACTGGCGGGAAGAATTTACCGTTCTGCGGCTACATTGGGACTGCCCCGCCGGGGATATTCTCTGGCGCGATGGT‑CCCATGTTTTTTGATCTGGATGATGCACGTAATGGTCCAGCCGGTCAGGATTTTTGGGTGtt > 2:449550/1‑150 (MQ=255) ccgTTACAGCACACTGGCGGGAAGATTTTACCCTTCTGCGGCTACATGGAGACTGCCACGCCGGGGATATTTTCTGGCGCGGTGGT‑CCAATGTTTGTTGATCTGGGTGATGCACGGAATGGTCCCGCCGTTCCGGGTTTGTGGGTGtt > 1:587237/1‑148 (MQ=255) tACAGCACACTGGCGGGAAGATTTTATCGTTTTTCGGCTACATGGGGACTACCACGCCGGGGATATTTTCTGGCGCGGTGGT‑TCCATGTTTGTTGTTTTGGGTGATGCACGTAATGGTCCAGCCGTTCTGGGTTTTTGGGTGtt > 1:1004486/1‑144 (MQ=255) gCACACTGGCGGGAAGATTTTACCGTTTTTCGGCGACATGGAGACCGCCACCCCGGGAATATTTTCTGGCGCGATGGG‑CCAATGTTTGTTGATCTGGATGAAGCACGTAATGGTCCAGCCGTTCTGGAATTGTGGATGTTTCTCAATAGCg > 1:139738/1‑151 (MQ=255) gggAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGT‑CCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCg > 2:1034283/1‑150 (MQ=255) aaGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGT‑CCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGggca < 2:994845/151‑1 (MQ=255) ttCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGT‑CCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCgg < 1:684826/150‑1 (MQ=255) cACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGT‑CCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATg < 1:865564/151‑1 (MQ=255) cACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGT‑CCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATg > 1:4070/1‑151 (MQ=255) | CCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGT‑CCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATG > NC_000913/4042851‑4043143 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |