Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,622,250	0	A	G	52.6%	24.2 / 48.4	38	E161G (GAG→GGG)	yjjJ	putative protein kinase
Reads supporting (aligned to +/- strand):  ref base A (5/13);  new base G (20/0);  total (25/13)
Fisher's exact test for biased strand distribution p-value = 1.58e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.13e-06

CGCAGGGCTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTTGGTTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGC‑GGAAATTCCTCTGGATCAAAAACTCACCCATTACGAACAGCTGGCAAGTGATGCACTGGCAGGA‑GAAATTGTGGGTTCTTCTGCGGGCGGCGAGCAGCCAAAATTTACCT  >  NC_000913/4622103‑4622399                                                                                                                                                    |                                                                                                                                                        cGCAGGGCTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTTGGTTGGTCggggaggg                                                                                                                                                      <  1:313023/151‑1 (MQ=255)   cAGGGCTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTTGGTTGGTCggggagggga                                                                                                                                                    <  1:527659/151‑1 (MQ=255)       gCTTTTTAGGGCTCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGGCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGGTGGGTGGTGGGGGGGGGGAAt                                                                                                                                                  >  1:563317/1‑149 (MQ=255)        cTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTTGGTTGGTCGGGGGGGGGGAatat                                                                                                                                               >  2:603849/1‑151 (MQ=255)        cTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTTGGGTTGGGGGGGGGGGGGATTAt                                                                                                                                               >  1:327781/1‑151 (MQ=255)                                gAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTTGGTTGGGCGGGGGGGGGGATTATCAGCGATGGGTTACTGCacaaca                                                                                                                        >  1:351457/1‑150 (MQ=255)                                     tAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTTGGTTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTg                                                                                                                   <  2:63747/150‑1 (MQ=255)                                      aGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTTGGGTGGGCGGGGGGGGGGATTATTAGCGATGGGTTTCTGCACAACAccccg                                                                                                                   >  1:523091/1‑147 (MQ=255)                                          gcgcAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTTGGTTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGC‑GGaa                                                                                                             <  2:435466/150‑1 (MQ=255)                                          gcgcAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTTGGGTGGGCGGGGGGGGGGATTATAAGAGGTGGATTACTGCACAAAAccccggggga                                                                                                              >  1:435434/1‑143 (MQ=255)                                           cgcAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTTGGTTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGC‑GGAAAt                                                                                                           <  1:476349/151‑1 (MQ=255)                                              aaCTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTTTGTTGGTCGGGGGGGGGGAATATCAGGGATGGGTTACTTCACAACACCCTGC‑GGGAAATcc                                                                                                        >  2:25264/1‑151 (MQ=255)                                                  gAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTTGGTTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGC‑GGAAATTCctct                                                                                                     <  2:715010/150‑1 (MQ=255)                                                  gAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTTGGTTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGC‑GGAAATTCCTCTg                                                                                                    <  2:369163/151‑1 (MQ=255)                                                        gactgaTGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTTGGTTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGC‑GGAAATTCCTCTGGATCa                                                                                               <  2:529074/150‑1 (MQ=255)                                                        gactgaTGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTTGGTTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGC‑GGAAATTCCTCTGGATCa                                                                                               <  2:112947/150‑1 (MQ=255)                                                                atatACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTTTGTTGGTCGGGGGGGGGGAATATCAGCGATGGATTACTTCACAACACCCTGC‑GGAAATTCCTCTGGATCaaaaa                                                                                           >  2:667055/1‑146 (MQ=255)                                                                     cGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTTGGTTGGTCGGGGGGGGGGATTATCAGCGATGGATTACTGCACAACAACCCGC‑GGGAATTCCTCTGGGTCAAAAAATCAccc                                                                                    >  1:661357/1‑148 (MQ=255)                                                                      gTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTTGGGTGGTCGGGGGGGGGGATTATCAGCGATGGGTTAACTCACCACACCCTGC‑GGGAATTTCTCTGGATCAAAAACTCACCCCtt                                                                                 >  2:760376/1‑150 (MQ=255)                                                                       tctcTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTTGGGTTGTCGGGGGGGGGGATTATCAGCGATGGATTACTGCACAAAACCCCGC‑GGGAATTTCTCTTGATCAAAAAATCAccc                                                                                    >  1:18313/1‑146 (MQ=255)                                                                        ctctGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTTGGGTGGTCGGGGGGGGGGATTATCAGCGATGGATTACTGCACAACACCCTGC‑GGAAAATTCTCTGGATCAAAAAATCACCCAtt                                                                                 >  2:739172/1‑148 (MQ=255)                                                                           tGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTTGGTTGGTCGGGGGGGGGGATTATCAGCGATGGGTTACTGCACAACACCCTGC‑GGGAATTCCTCTGGATCAAAAAATCACCCCTTACGaa                                                                            >  1:619857/1‑150 (MQ=255)                                                                            ggCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTTGGTTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGC‑GGAAATTCCTCTGGATCAAAAACTCACCCATTACGaa                                                                            <  2:68990/149‑1 (MQ=255)                                                                            ggCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTTGGTTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGC‑GGAAATTCCTCTGGATCAAAAACTCACCCATTACGa                                                                             <  2:81282/148‑1 (MQ=255)                                                                              cAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTTGGTTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGC‑GGAAATTCCTCTGGATCAAAAACTCACCCATTACGAACAGc                                                                        <  2:269591/151‑1 (MQ=255)                                                                              cAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTTGGGTGGTCGGGGGGGGGGATTATCAGCGATGGATTACTGCACAACACCCCGC‑GGAAAATCCTCTGGGTCAAAAAATCACCCATTACGAACa                                                                          >  1:511800/1‑149 (MQ=255)                                                                                  aagaagATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTTGGGTGGTCGGGGGGGGGGATTATCAGCGATGGGTTACTGCACAACACCCTGC‑GGGAATTCCTCTGGATCAAAAACTCACCCATTACGAACAGCTgg                                                                     >  2:270684/1‑150 (MQ=255)                                                                                    gaagaTGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTTTGGTGGGCGGGGGGGGGGATTTTCAGCGGTGGATTAATTCACCAAACCCTGC‑GGGAAATTCTCTGGGTCAAAAAATCACCCCTTACGAACaaccgg                                                                     >  1:322356/1‑143 (MQ=255)                                                                                     aagaTGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTTTGTTGGGCGGGGGGGGGGATTATCAGCGATGGATTACTGCACAAAACCCCGC‑GGGAATTCCTCTGGGTCAAAAAATCACCCATTACGAAAAGCCGGCa                                                                   >  1:745035/1‑149 (MQ=255)                                                                                         tgtgCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGATGGTTGGGGGGGGGGGGGGATTATCAGCGGTGGATTTCTGCACAACACCCTGC‑GGAAATTCCTCTTGGTCAAAAACTCACCCATTTCGAAAAACTGGCAAGt                                                                >  2:772881/1‑148 (MQ=255)                                                                                               ctACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTTGGTTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGC‑GGAAATTCCTCTGGATCAAAAACTCACCCATTACGAACAGCTGGCAAGTGATGCAc                                                         <  2:139926/149‑1 (MQ=255)                                                                                                tACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTTTGTTGGTCGGGGGGGGGGATTTTCAGCGATGGATTTCTGCACAACACCCCGC‑GGGAATTTCTCTGGATCAAAAAATCACCCATTACGAACAGCTGGGCAGGGATGCACTgg                                                      >  1:301992/1‑151 (MQ=255)                                                                                                        gACCGTATTTAACGGTGAATATACTGGCGGTTGGTTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGC‑GGAAATTCCTCTGGATCAAAAACTCACCCATTACGAACAGCTGGCAAGTGATGCACTGGCAGga‑ga                                              <  1:150099/150‑1 (MQ=255)                                                                                                              aTTTAACGGTGAATATACTGGCGGTTGGTTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGC‑GGAAATTCCTCTGGATCAAAAACTCACCCATTACGAACAGCTGGCAAGTGATGCACTGGCAGGA‑GAAATtgt                                        >  2:663662/1‑150 (MQ=255)                                                                                                                 tAACGGTGAATATACTGGCGGTTGGGTGGTCGGGGAGGGGGATTATCAGCGATGGGTTACTGCACAACACCCTGC‑GGAAATTTCTCTGGATCAAAAACTCACCCCTTAAGAACAACTGGCAAGTGATGCACAGGCAGGA‑GAAATTGTggg                                     >  2:610462/1‑150 (MQ=255)                                                                                                                    cGGTGAATATACTGGCGGTTTGTTGGTGGGGGGGGGGGATTATCAGCGGTGGAGTTCTGCACAACACCCTGCGGGAAATCCTTCTGAAT‑AAAAACTCACCCCTTAAGAACAACTGGCAAGGGATGCACCGGCCGGAGGAAATTGGGGGtct                                  >  1:203293/1‑149 (MQ=255)                                                                                                                                tGGCGGTTGGTTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGC‑GGAAATTCCTCTGGATCAAAAACTCACCCATTACGAACAGCTGGCAAGTGATGCACTGGCAGGA‑GAAATTGTGGGTTCTTCTgcgggcgg                      >  1:320260/1‑150 (MQ=255)                                                                                                                                 ggCGGTTGGTTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGC‑GGAAATTCCTCTGGATCAAAAACTCACCCATTACGAACAGCTGGCAAGTGATGCACTGGCAGGA‑GAAATTGTGGGTTCTTCTGCGGgcggcg                    >  2:99021/1‑151 (MQ=255)                                                                                                                                           tggtCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGC‑GGAAATTCCTCTGGATCAAAAACCCACCCATTACGAATAGCTGGCAAGTGATGCACTGGCAGGA‑GAAATTGTGGGTTCTTCTGCGGGCGGCGAGCAGCCaa           >  1:752488/1‑150 (MQ=255)                                                                                                                                            ggtCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGC‑GGAAATTCCTCTGGATCAAAAACTCACCCATTACGAACAGCTGGCAAGTGATGCACTGGCAGGA‑GAAATTGTGGGTTCTTCTGCGGGCGGCGAGCAGCCaaa          <  1:177672/150‑1 (MQ=255)                                                                                                                                                   gaggggaATTATCAGCAATGGATTACTGCACAACACCCTGC‑GGAAATTCCTCTGGATCAAAAACTCACCCATTACGAACAGCTGGCAAGTGATGCACTGGCATGA‑GAAATTGTGGGTTCTTCTGCGGGCGGCGAGCAGCCAAAATGTACct  <  1:250997/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                        CGCAGGGCTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTTGGTTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGC‑GGAAATTCCTCTGGATCAAAAACTCACCCATTACGAACAGCTGGCAAGTGATGCACTGGCAGGA‑GAAATTGTGGGTTCTTCTGCGGGCGGCGAGCAGCCAAAATTTACCT  >  NC_000913/4622103‑4622399

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑